AU737449B2 - FEN-1 endonucleases, mixtures and cleavage methods - Google Patents

FEN-1 endonucleases, mixtures and cleavage methods Download PDF

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Publication number
AU737449B2
AU737449B2 AU55898/98A AU5589898A AU737449B2 AU 737449 B2 AU737449 B2 AU 737449B2 AU 55898/98 A AU55898/98 A AU 55898/98A AU 5589898 A AU5589898 A AU 5589898A AU 737449 B2 AU737449 B2 AU 737449B2
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AU
Australia
Prior art keywords
cleavage
fen
oligonucleotide
nucleic acid
endonuclease
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired
Application number
AU55898/98A
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English (en)
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AU5589898A (en
Inventor
Michael W. Kaiser
Victor I. Lyamichev
Natasha Lyamicheva
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Third Wave Technologies Inc
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Third Wave Technologies Inc
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Filing date
Publication date
Priority claimed from US08/757,653 external-priority patent/US5843669A/en
Priority claimed from US08/758,314 external-priority patent/US6090606A/en
Application filed by Third Wave Technologies Inc filed Critical Third Wave Technologies Inc
Publication of AU5589898A publication Critical patent/AU5589898A/en
Application granted granted Critical
Publication of AU737449B2 publication Critical patent/AU737449B2/en
Priority to AU93417/01A priority Critical patent/AU779443B2/en
Anticipated expiration legal-status Critical
Expired legal-status Critical Current

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
    • C12N9/22—Ribonucleases [RNase]; Deoxyribonucleases [DNase]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6816—Hybridisation assays characterised by the detection means
    • C12Q1/6823—Release of bound markers
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10S435/00—Chemistry: molecular biology and microbiology
    • Y10S435/975—Kit

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  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Genetics & Genomics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Molecular Biology (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Biochemistry (AREA)
  • Physics & Mathematics (AREA)
  • Analytical Chemistry (AREA)
  • Immunology (AREA)
  • Biophysics (AREA)
  • Medicinal Chemistry (AREA)
  • Biomedical Technology (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Saccharide Compounds (AREA)
AU55898/98A 1996-11-29 1997-11-26 FEN-1 endonucleases, mixtures and cleavage methods Expired AU737449B2 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
AU93417/01A AU779443B2 (en) 1996-11-29 2001-11-23 Fen-1 endonucleases, mixtures and cleavage methods

Applications Claiming Priority (5)

Application Number Priority Date Filing Date Title
US08/757653 1996-11-29
US08/757,653 US5843669A (en) 1996-01-24 1996-11-29 Cleavage of nucleic acid acid using thermostable methoanococcus jannaschii FEN-1 endonucleases
US08/758314 1996-12-02
US08/758,314 US6090606A (en) 1996-01-24 1996-12-02 Cleavage agents
PCT/US1997/021783 WO1998023774A1 (en) 1996-11-29 1997-11-26 Fen-1 endonucleases, mixtures and cleavage methods

Related Child Applications (1)

Application Number Title Priority Date Filing Date
AU93417/01A Division AU779443B2 (en) 1996-11-29 2001-11-23 Fen-1 endonucleases, mixtures and cleavage methods

Publications (2)

Publication Number Publication Date
AU5589898A AU5589898A (en) 1998-06-22
AU737449B2 true AU737449B2 (en) 2001-08-23

Family

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Family Applications (1)

Application Number Title Priority Date Filing Date
AU55898/98A Expired AU737449B2 (en) 1996-11-29 1997-11-26 FEN-1 endonucleases, mixtures and cleavage methods

Country Status (10)

Country Link
US (2) US6562611B1 (cg-RX-API-DMAC7.html)
EP (3) EP0966542B1 (cg-RX-API-DMAC7.html)
JP (4) JP4362150B2 (cg-RX-API-DMAC7.html)
AT (2) ATE530652T1 (cg-RX-API-DMAC7.html)
AU (1) AU737449B2 (cg-RX-API-DMAC7.html)
CA (1) CA2273204C (cg-RX-API-DMAC7.html)
DE (1) DE69739108D1 (cg-RX-API-DMAC7.html)
DK (1) DK0966542T3 (cg-RX-API-DMAC7.html)
ES (2) ES2375764T3 (cg-RX-API-DMAC7.html)
WO (1) WO1998023774A1 (cg-RX-API-DMAC7.html)

Families Citing this family (100)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6759226B1 (en) 2000-05-24 2004-07-06 Third Wave Technologies, Inc. Enzymes for the detection of specific nucleic acid sequences
US5846717A (en) * 1996-01-24 1998-12-08 Third Wave Technologies, Inc. Detection of nucleic acid sequences by invader-directed cleavage
US7150982B2 (en) 1991-09-09 2006-12-19 Third Wave Technologies, Inc. RNA detection assays
US5985557A (en) * 1996-01-24 1999-11-16 Third Wave Technologies, Inc. Invasive cleavage of nucleic acids
WO1998023774A1 (en) * 1996-11-29 1998-06-04 Third Wave Technologies, Inc. Fen-1 endonucleases, mixtures and cleavage methods
US6780982B2 (en) 1996-07-12 2004-08-24 Third Wave Technologies, Inc. Charge tags and the separation of nucleic acid molecules
US6210880B1 (en) 1997-09-19 2001-04-03 Third Wave Technologies, Inc. Polymorphism analysis by nucleic acid structure probing with structure-bridging oligonucleotides
US7101672B2 (en) 1998-05-05 2006-09-05 Third Wave Technologies, Inc. Target-dependent reactions using structure-bridging oligonucleotides
AU744369B2 (en) 1997-05-05 2002-02-21 Third Wave Technologies, Inc. Target-dependent reactions using structure-bridging oligonucleotides
US6194149B1 (en) 1998-03-03 2001-02-27 Third Wave Technologies, Inc. Target-dependent reactions using structure-bridging oligonucleotides
WO2000071559A1 (en) 1999-05-24 2000-11-30 Invitrogen Corporation Method for deblocking of labeled oligonucleotides
US7118860B2 (en) 1999-10-29 2006-10-10 Stratagene California Methods for detection of a target nucleic acid by capture
US7824859B2 (en) * 1999-10-29 2010-11-02 Cytyc Corporation Methods for detection of a target nucleic acid by forming a cleavage structure using an RNA polymerase
US7838225B2 (en) 1999-10-29 2010-11-23 Hologic, Inc. Methods for detection of a target nucleic acid by forming a cleavage structure using a reverse transcriptase
US7534568B2 (en) 1999-10-29 2009-05-19 Hologic Inc. Methods for detection of a target nucleic acid by forming a cleavage structure with a cleavage resistant probe
US6528254B1 (en) 1999-10-29 2003-03-04 Stratagene Methods for detection of a target nucleic acid sequence
WO2001061015A2 (en) * 2000-02-17 2001-08-23 Qiagen Gmbh Thermostable chimeric nucleic acid polymerases and uses thereof
EP1276904B1 (en) * 2000-03-22 2013-08-28 Life Technologies Corporation Methods of using semiconductor nanocrystals in bead-based nucleic acid assays
US6964848B2 (en) 2000-03-27 2005-11-15 Applera Corporation Invasion assay
EP1294863B1 (en) * 2000-05-24 2009-07-08 Third Wave Technologies, Inc. Detection of rna
US6511810B2 (en) 2000-07-03 2003-01-28 Applera Corporation Polynucleotide sequence assay
DE10049211A1 (de) * 2000-10-05 2002-04-18 Qiagen Gmbh Thermostabile Polymerase aus Thermococcus pacificus
US6350580B1 (en) 2000-10-11 2002-02-26 Stratagene Methods for detection of a target nucleic acid using a probe comprising secondary structure
CA2428798C (en) * 2000-11-15 2013-09-03 Third Wave Technologies, Inc. Fen-1 endonucleases from archaeoglobus veneficus
DE10131441A1 (de) * 2001-06-29 2003-01-30 Henkel Kgaa Eine neue Gruppe von alpha-Amylasen sowie ein Verfahren zur Identifizierung und Gewinnung neuer alpha-Amylasen
GB0205455D0 (en) 2002-03-07 2002-04-24 Molecular Sensing Plc Nucleic acid probes, their synthesis and use
US7312033B2 (en) 2002-11-14 2007-12-25 Third Wave Technologies, Inc. CFTR allele detection assays
JP4230457B2 (ja) 2002-12-18 2009-02-25 サード・ウェーブ・テクノロジーズ・インク 低分子核酸の検出方法
US8206904B2 (en) 2002-12-18 2012-06-26 Third Wave Technologies, Inc. Detection of nucleic acids
US7700750B2 (en) 2003-01-09 2010-04-20 Third Wave Technologies, Inc. Connexin allele detection assays
US20040161749A1 (en) 2003-01-24 2004-08-19 Hall Jeff G. 3' end tagged oligonucleotides
CN100482794C (zh) 2003-07-29 2009-04-29 大制药株式会社 用于判定药物性粒细胞过少症发病几率的寡核苷酸、检测用试剂盒及其用途
JP5138230B2 (ja) 2004-01-07 2013-02-06 サード・ウェーブ・テクノロジーズ・インク C型肝炎ウイルスの遺伝子型の決定方法
US7309589B2 (en) * 2004-08-20 2007-12-18 Vironix Llc Sensitive detection of bacteria by improved nested polymerase chain reaction targeting the 16S ribosomal RNA gene and identification of bacterial species by amplicon sequencing
EP2354228A3 (en) 2004-10-28 2011-11-02 Otsuka Pharmaceutical Company, Limited Identification marker responsive to interferon therapy for renal cell cancer
US7727721B2 (en) 2005-03-08 2010-06-01 California Institute Of Technology Hybridization chain reaction amplification for in situ imaging
WO2007044727A2 (en) 2005-10-07 2007-04-19 California Institute Of Technology Pkr activation via hybridization chain reaction
US7482127B2 (en) 2005-11-03 2009-01-27 Third Wave Technologies, Inc. Nucleic acid detection assays employing blocker oligonucleotides
JPWO2007111324A1 (ja) * 2006-03-29 2009-08-13 国立大学法人京都大学 遺伝子中の5−メチルシトシン検出方法および検出キット
CN103146825B (zh) 2006-06-01 2017-08-25 第三次浪潮技术公司 核酸的检测
US7759062B2 (en) 2006-06-09 2010-07-20 Third Wave Technologies, Inc. T-structure invasive cleavage assays, consistent nucleic acid dispensing, and low level target nucleic acid detection
EP2079754B1 (en) 2006-10-04 2017-03-15 Third Wave Technologies, Inc. Snap-back primers and detectable hairpin structures
WO2008144562A1 (en) 2007-05-16 2008-11-27 California Institute Of Technology A versatile nucleic acid hairpin motif for programming biomolecular self-assembly pathways
US8497364B2 (en) 2008-02-27 2013-07-30 California Institute Of Technology Triggered RNAi
BRPI0908748A2 (pt) 2008-03-15 2015-07-28 Hologic Inc Composições e métodos para análise de moléculas de ácido nucleico durante reações de amplificação
US20090246788A1 (en) 2008-04-01 2009-10-01 Roche Nimblegen, Inc. Methods and Assays for Capture of Nucleic Acids
WO2009155271A1 (en) * 2008-06-17 2009-12-23 Sigma-Aldrich Co. Real time polymerase chain reaction process using a universal detection system
US8658780B2 (en) 2010-05-18 2014-02-25 California Institute Of Technology Triggered covalent probes for imaging and silencing genetic expression
US8962241B2 (en) 2010-07-20 2015-02-24 California Institute Of Technology Triggered molecular geometry based bioimaging probes
US9834439B2 (en) 2010-07-20 2017-12-05 California Institute Of Technology Biomolecular self-assembly
US8877438B2 (en) 2010-07-20 2014-11-04 California Institute Of Technology Self-assembled polynucleotide structure
US8361720B2 (en) 2010-11-15 2013-01-29 Exact Sciences Corporation Real time cleavage assay
US8715937B2 (en) 2010-11-15 2014-05-06 Exact Sciences Corporation Mutation detection assay
US8916344B2 (en) 2010-11-15 2014-12-23 Exact Sciences Corporation Methylation assay
MX352460B (es) * 2011-01-11 2017-11-24 Seegene Inc Detección de secuencias de ácido nucleico objetivo mediante ensayo de escisión y extensión del pto.
US11078525B2 (en) 2011-03-29 2021-08-03 Seegene, Inc. Detection of target nucleic acid sequence by PTO cleavage and extension-dependent cleavage
WO2012150749A1 (en) 2011-05-04 2012-11-08 Seegene, Inc. Detection of target nucleic acid sequences by po cleavage and hybridization
WO2013058868A2 (en) 2011-10-18 2013-04-25 Exact Sciences Corporation Multiplexed kras mutation detection assay
KR20130101952A (ko) 2012-02-02 2013-09-16 주식회사 씨젠 Pto 절단과 연장-의존적 혼성화를 이용한 타겟 핵산서열의 검출
CA2864523C (en) 2012-03-05 2019-02-05 Seegene, Inc. Detection of nucleotide variation on target nucleic acid sequence by pto cleavage and extension assay
WO2014158628A1 (en) 2013-03-14 2014-10-02 Hologic, Inc. Compositions and methods for analysis of nucleic acid molecules
US9856472B2 (en) 2013-07-01 2018-01-02 California Institute Of Technology Small conditional RNAs
KR102160389B1 (ko) 2013-08-05 2020-09-28 트위스트 바이오사이언스 코포레이션 드 노보 합성된 유전자 라이브러리
CA2975852A1 (en) 2015-02-04 2016-08-11 Twist Bioscience Corporation Methods and devices for de novo oligonucleic acid assembly
WO2016172377A1 (en) 2015-04-21 2016-10-27 Twist Bioscience Corporation Devices and methods for oligonucleic acid library synthesis
AU2016257426B2 (en) 2015-05-01 2020-01-16 Gen-Probe Incorporated Multiplex invasive cleavage assays
KR20180050411A (ko) 2015-09-18 2018-05-14 트위스트 바이오사이언스 코포레이션 올리고핵산 변이체 라이브러리 및 그의 합성
WO2017053450A1 (en) 2015-09-22 2017-03-30 Twist Bioscience Corporation Flexible substrates for nucleic acid synthesis
EP3384077A4 (en) 2015-12-01 2019-05-08 Twist Bioscience Corporation FUNCTIONALIZED SURFACES AND MANUFACTURE THEREOF
ES2908919T3 (es) 2016-07-05 2022-05-04 California Inst Of Techn Reacción en cadena de hibridación de iniciador fraccionario
WO2018038772A1 (en) 2016-08-22 2018-03-01 Twist Bioscience Corporation De novo synthesized nucleic acid libraries
AU2017321492A1 (en) 2016-08-30 2019-03-14 California Institute Of Technology Immunohistochemistry via hybridization chain reaction
EP4664304A3 (en) 2016-09-21 2026-01-21 Atlas Data Storage, Inc. Nucleic acid based data storage
GB2573069A (en) 2016-12-16 2019-10-23 Twist Bioscience Corp Variant libraries of the immunological synapse and synthesis thereof
WO2018156792A1 (en) 2017-02-22 2018-08-30 Twist Bioscience Corporation Nucleic acid based data storage
CA3056388A1 (en) 2017-03-15 2018-09-20 Twist Bioscience Corporation Variant libraries of the immunological synapse and synthesis thereof
CN107012241B (zh) * 2017-05-09 2020-11-20 东莞微量精准检测研究院有限公司 一种U6、miR 92a及miR 21的三重RT-qPCR检测方法及试剂盒
IL271205B2 (en) 2017-06-12 2025-02-01 Twist Bioscience Corp Methods for assembling continuous nucleic acids
WO2018231864A1 (en) 2017-06-12 2018-12-20 Twist Bioscience Corporation Methods for seamless nucleic acid assembly
CN110770355A (zh) * 2017-07-31 2020-02-07 凸版印刷株式会社 核酸检测方法及检测用试剂
EP3665309B1 (en) 2017-08-11 2023-02-22 Gen-Probe Incorporated Compositions and methods for detecting staphylococcus aureus
CN111566125A (zh) 2017-09-11 2020-08-21 特韦斯特生物科学公司 Gpcr结合蛋白及其合成
WO2019066461A2 (en) 2017-09-29 2019-04-04 Seegene, Inc. DETECTION OF TARGET NUCLEIC ACID SEQUENCES BY CLEAVAGE ANALYSIS AND EXTENSION OF PTO
CN111565834B (zh) 2017-10-20 2022-08-26 特韦斯特生物科学公司 用于多核苷酸合成的加热的纳米孔
US11662281B2 (en) 2017-12-13 2023-05-30 Gen-Probe Incorporated Compositions and methods for biological sample processing
EP3724354A1 (en) 2017-12-15 2020-10-21 Gen-Probe Incorporated Compositions and methods for detecting toxigenic clostridium difficile
IL312616B2 (en) 2018-01-04 2025-08-01 Twist Bioscience Corp Dna-based digital information storage
EP3814497A4 (en) 2018-05-18 2022-03-02 Twist Bioscience Corporation POLYNUCLEOTIDES, REAGENTS AND METHODS FOR NUCLEIC ACID HYBRIDIZATION
EP3902816A4 (en) 2018-12-26 2022-09-14 Twist Bioscience Corporation HIGH-PRECISION DE NOVO POLYNUCLEOTIDE SYNTHESIS
EP3914732A1 (en) 2019-01-25 2021-12-01 Gen-Probe Incorporated Detection of drug-resistant mycoplasma genitalium
CA3131689A1 (en) 2019-02-26 2020-09-03 Twist Bioscience Corporation Variant nucleic acid libraries for glp1 receptor
KR20210144698A (ko) 2019-02-26 2021-11-30 트위스트 바이오사이언스 코포레이션 항체 최적화를 위한 변이 핵산 라이브러리
EP3987019A4 (en) 2019-06-21 2023-04-19 Twist Bioscience Corporation Barcode-based nucleic acid sequence assembly
EP4031683A4 (en) 2019-09-16 2024-01-17 Exact Sciences Corporation Structure and temperature-dependent flap endonuclease substrates
US12091777B2 (en) 2019-09-23 2024-09-17 Twist Bioscience Corporation Variant nucleic acid libraries for CRTH2
CN115023440B (zh) 2019-09-23 2025-09-12 特韦斯特生物科学公司 结合CD3ε的抗体
AU2020400030B2 (en) 2019-12-09 2026-05-21 Twist Bioscience Corporation Variant nucleic acid libraries for adenosine receptors
EP4284925A4 (en) 2021-01-26 2025-08-13 California Inst Of Techn ALLOSTERIC CONDITIONAL GUIDE RNAS FOR SELECTIVE REGULATION OF CRISPR/CAS CELLS
CA3232383C (en) 2021-09-30 2026-05-12 Gen-Probe Incorporated Temperature-selectable fret cassette signaling
WO2024054924A1 (en) 2022-09-08 2024-03-14 Gen-Probe Incorporated Method of detecting nucleic acid analytes using dual-specificity primers

Family Cites Families (41)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4518526A (en) 1982-12-22 1985-05-21 Genentech, Inc. Purification and activity assurance of precipitated heterologous proteins
US4511503A (en) 1982-12-22 1985-04-16 Genentech, Inc. Purification and activity assurance of precipitated heterologous proteins
US4511502A (en) 1982-12-22 1985-04-16 Genentech, Inc. Purification and activity assurance of precipitated heterologous proteins
US4512922A (en) 1982-12-22 1985-04-23 Genentech, Inc. Purification and activity assurance of precipitated heterologous proteins
US4683194A (en) 1984-05-29 1987-07-28 Cetus Corporation Method for detection of polymorphic restriction sites and nucleic acid sequences
US4775619A (en) 1984-10-16 1988-10-04 Chiron Corporation Polynucleotide determination with selectable cleavage sites
US5118605A (en) 1984-10-16 1992-06-02 Chiron Corporation Polynucleotide determination with selectable cleavage sites
US4683195A (en) 1986-01-30 1987-07-28 Cetus Corporation Process for amplifying, detecting, and/or-cloning nucleic acid sequences
US4683202A (en) 1985-03-28 1987-07-28 Cetus Corporation Process for amplifying nucleic acid sequences
US5011769A (en) 1985-12-05 1991-04-30 Meiogenics U.S. Limited Partnership Methods for detecting nucleic acid sequences
US4876187A (en) 1985-12-05 1989-10-24 Meiogenics, Inc. Nucleic acid compositions with scissile linkage useful for detecting nucleic acid sequences
US5403711A (en) 1987-11-30 1995-04-04 University Of Iowa Research Foundation Nucleic acid hybridization and amplification method for detection of specific sequences in which a complementary labeled nucleic acid probe is cleaved
EP0365627B1 (en) 1988-03-24 1993-12-22 University Of Iowa Research Foundation Catalytic hybridization systems for the detection of nucleic acid sequences based on their activity as cofactors in catalytic reactions in which a complementary labeled nucleic acid probe is cleaved
ATE138106T1 (de) 1988-07-20 1996-06-15 David Segev Verfahren zur amplifizierung und zum nachweis von nukleinsäuresequenzen
WO1990010691A1 (en) 1989-03-10 1990-09-20 Grace-Sierra Horticultural Products Company Tissue culturing
US5144019A (en) 1989-06-21 1992-09-01 City Of Hope Ribozyme cleavage of HIV-I RNA
CA1337639C (en) 1989-08-01 1995-11-28 Joseph Eugene Celebuski Dna probe assay using neutrally charged probe strands
US5108892A (en) 1989-08-03 1992-04-28 Promega Corporation Method of using a taq dna polymerase without 5'-3'-exonuclease activity
AU646430B2 (en) 1989-12-22 1994-02-24 F. Hoffmann-La Roche Ag Recombinant expression vectors and purification methods for thermus thermophilus DNA polymerase
US5427930A (en) 1990-01-26 1995-06-27 Abbott Laboratories Amplification of target nucleic acids using gap filling ligase chain reaction
US5494810A (en) 1990-05-03 1996-02-27 Cornell Research Foundation, Inc. Thermostable ligase-mediated DNA amplifications system for the detection of genetic disease
US5210015A (en) 1990-08-06 1993-05-11 Hoffman-La Roche Inc. Homogeneous assay system using the nuclease activity of a nucleic acid polymerase
JP2709311B2 (ja) 1990-09-28 1998-02-04 エフ.ホフマン−ラ ロシュ アクチェンゲゼルシャフト 熱安定性dnaポリメラーゼの5→3′のエキソヌクレアーゼ突然変異
US6083686A (en) 1990-10-26 2000-07-04 Johnson & Johnson Clinical Diagnostic Systems, Inc. Increased production of Thermus aquaticus DNA polymerase in E. coli
US5994069A (en) 1996-01-24 1999-11-30 Third Wave Technologies, Inc. Detection of nucleic acids by multiple sequential invasive cleavages
US5846717A (en) 1996-01-24 1998-12-08 Third Wave Technologies, Inc. Detection of nucleic acid sequences by invader-directed cleavage
US5541311A (en) * 1992-12-07 1996-07-30 Third Wave Technologies, Inc. Nucleic acid encoding synthesis-deficient thermostable DNA polymerase
US5422253A (en) 1992-12-07 1995-06-06 Wisconsin Alumni Research Foundation Method of site specific nucleic acid cleavage
US5888780A (en) 1992-12-07 1999-03-30 Third Wave Technologies, Inc. Rapid detection and identification of nucleic acid variants
AU8102694A (en) 1993-11-17 1995-06-06 Id Biomedical Corporation Cycling probe cleavage detection of nucleic acid sequences
CA2188660C (en) 1994-04-25 2005-01-18 Richard G. H. Cotton Detection of mutation by resolvase cleavage
EP0705905B1 (de) 1994-07-16 2001-10-10 Roche Diagnostics GmbH Verfahren zum sensitiven Nachweis von Nukleinsäuren
WO1996015267A1 (en) * 1994-11-09 1996-05-23 Third Wave Technologies, Inc. Rapid detection and identification of nucleic acid variants and pathogens
DE4441626A1 (de) 1994-11-23 1996-05-30 Boehringer Mannheim Gmbh Verfahren zum besonders sensitiven Nachweis von Nukleinsäuren
US5545729A (en) 1994-12-22 1996-08-13 Hybridon, Inc. Stabilized ribozyme analogs
DE69528670T2 (de) 1994-12-23 2004-03-11 Dade Behring Inc., Deerfield Nachweis von nukleinsäuren durch nuklease-katalysierte produktbildung
US5874283A (en) 1995-05-30 1999-02-23 John Joseph Harrington Mammalian flap-specific endonuclease
US5882867A (en) 1995-06-07 1999-03-16 Dade Behring Marburg Gmbh Detection of nucleic acids by formation of template-dependent product
US5985557A (en) 1996-01-24 1999-11-16 Third Wave Technologies, Inc. Invasive cleavage of nucleic acids
EP1634890B1 (en) * 1996-01-24 2008-11-19 Third Wave Technologies, Inc. Invasive cleavage of nucleic acids
WO1998023774A1 (en) * 1996-11-29 1998-06-04 Third Wave Technologies, Inc. Fen-1 endonucleases, mixtures and cleavage methods

Non-Patent Citations (3)

* Cited by examiner, † Cited by third party
Title
BULT ET AL (1996) SCIENCE 273, 1058-73 *
HARRINGTON ET AL (1994) GENES & DEV. 8, 1344-55 *
MURRAY ET AL (1994) MOL. CELL BIOL. 14, 4878-88 *

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DK0966542T3 (da) 2009-03-23
ATE414167T1 (de) 2008-11-15
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EP2055781A2 (en) 2009-05-06
WO1998023774A1 (en) 1998-06-04
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DE69739108D1 (de) 2008-12-24

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