AU731860B2 - Recombinant pox virus for immunization against tumor-associated antigens - Google Patents
Recombinant pox virus for immunization against tumor-associated antigens Download PDFInfo
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- AU731860B2 AU731860B2 AU37268/97A AU3726897A AU731860B2 AU 731860 B2 AU731860 B2 AU 731860B2 AU 37268/97 A AU37268/97 A AU 37268/97A AU 3726897 A AU3726897 A AU 3726897A AU 731860 B2 AU731860 B2 AU 731860B2
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- General Engineering & Computer Science (AREA)
- Biotechnology (AREA)
- Biomedical Technology (AREA)
- Microbiology (AREA)
- Public Health (AREA)
- Physics & Mathematics (AREA)
- Pharmacology & Pharmacy (AREA)
- Plant Pathology (AREA)
- Virology (AREA)
- Animal Behavior & Ethology (AREA)
- Veterinary Medicine (AREA)
- General Chemical & Material Sciences (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
- Cell Biology (AREA)
- Toxicology (AREA)
- Gastroenterology & Hepatology (AREA)
- Urology & Nephrology (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)
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Priority Applications (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| AU54194/01A AU767562B2 (en) | 1996-07-25 | 2001-07-04 | Recombinant pox virus for immunization against tumor-associated antigens |
| AU2003266471A AU2003266471A1 (en) | 1996-07-25 | 2003-12-04 | Recombinant pox virus for immunization against tumor-associated antigens |
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US68628096A | 1996-07-25 | 1996-07-25 | |
| US08/686280 | 1996-07-25 | ||
| PCT/US1997/012203 WO1998004727A1 (en) | 1996-07-25 | 1997-07-15 | Recombinant pox virus for immunization against tumor-associated antigens |
Related Child Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| AU54194/01A Division AU767562B2 (en) | 1996-07-25 | 2001-07-04 | Recombinant pox virus for immunization against tumor-associated antigens |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| AU3726897A AU3726897A (en) | 1998-02-20 |
| AU731860B2 true AU731860B2 (en) | 2001-04-05 |
Family
ID=24755674
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| AU37268/97A Ceased AU731860B2 (en) | 1996-07-25 | 1997-07-15 | Recombinant pox virus for immunization against tumor-associated antigens |
Country Status (6)
| Country | Link |
|---|---|
| US (1) | US7410644B2 (Direct) |
| EP (2) | EP0954593A1 (Direct) |
| JP (2) | JP4475683B2 (Direct) |
| AU (1) | AU731860B2 (Direct) |
| CA (1) | CA2261989C (Direct) |
| WO (1) | WO1998004727A1 (Direct) |
Families Citing this family (44)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPH09504000A (ja) * | 1993-08-11 | 1997-04-22 | ジェナー テクノロジーズ | 前立腺癌ワクチン |
| US20010036928A1 (en) * | 1996-04-22 | 2001-11-01 | Chamberlain Ronald S. | Heterologous boosting immunizations |
| US6969609B1 (en) | 1998-12-09 | 2005-11-29 | The United States Of America As Represented By The Department Of Health And Human Serivces | Recombinant vector expressing multiple costimulatory molecules and uses thereof |
| DE69924826T8 (de) * | 1998-11-18 | 2008-09-25 | Oxford Biomedica (Uk) Ltd. | Verwendung vom krebs-assoziierten antigen 5t4 zur immuntherapie |
| US7148035B1 (en) | 1998-11-18 | 2006-12-12 | Oxford Biomedica (Uk) Limited | Polypeptide |
| CA2354024C (en) * | 1998-12-09 | 2009-12-22 | Jeffrey Schlom | A recombinant vector expressing multiple costimulatory molecules and uses thereof |
| EP1865065A1 (en) * | 1998-12-09 | 2007-12-12 | The Government of the United States of America, as represented by the Secretary, Department of Health and Human Services | A recombinant vector expressing multiple costimulatory molecules and uses thereof |
| EP1496939B1 (en) * | 2002-04-09 | 2007-08-15 | Sanofi Pasteur Limited | Modified cea nucleic acid and expression vectors |
| JP2007502602A (ja) * | 2003-08-21 | 2007-02-15 | バイラックス・ディベロップメント・プロプライエタリー・リミテッド | 前立腺癌の治療用の前立腺特異的抗原をコードするポックスウィルスベクター |
| ATE506444T1 (de) * | 2003-10-08 | 2011-05-15 | Sanofi Pasteur Ltd | Modifizierter cea/b7-vektor |
| US8933041B2 (en) | 2003-11-12 | 2015-01-13 | The United States Of America, As Represented By The Secretary, Department Of Health And Human Services | System for treating and preventing breast cancer |
| US8901093B2 (en) | 2003-11-12 | 2014-12-02 | The United States Of America, As Represented By The Secretary Of The Department Of Health And Human Services | Custom vectors for treating and preventing pancreatic cancer |
| WO2010081766A1 (en) | 2009-01-13 | 2010-07-22 | Transgene Sa | Use of a saccharomyces cerevisiae mitochondrial nucleic acids fraction for immune stimulation |
| JP5379243B2 (ja) | 2009-01-20 | 2013-12-25 | トランジェーヌ、ソシエテ、アノニム | 患者を選択するためのバイオマーカーおよびそれに関連する方法 |
| JP5774578B2 (ja) | 2009-03-24 | 2015-09-09 | トランジェーヌ、ソシエテ、アノニムＴｒａｎｓｇｅｎｅ Ｓ．Ａ． | 患者をモニターするためのバイオマーカー |
| EP2419728B1 (en) | 2009-04-17 | 2014-01-08 | Transgene SA | Biomarker for monitoring patients |
| CA2767458A1 (en) | 2009-07-10 | 2011-01-13 | Bruce Acres | Biomarker for selecting patients and related methods |
| AU2011285534B2 (en) | 2010-08-06 | 2015-12-17 | The United States Of America, As Represented By The Secretary, Department Of Health And Human Services | Biomarkers for prostate cancer and methods for their detection |
| US9725494B2 (en) * | 2013-05-17 | 2017-08-08 | United States Of America, As Represented By The Secretary, Department Of Health And Human Services | Soluble CD27 (sCD27) and the use thereof |
| RU2714142C2 (ru) * | 2013-11-05 | 2020-02-12 | Бавариан Нордик А/С | Комбинированное лекарственное средство для лечения рака с использованием поксвируса, экспрессирующего опухолевый антиген, и антагониста и/или агониста ингибитора имунной контрольной точки |
| ES2851451T3 (es) | 2014-05-13 | 2021-09-07 | Bavarian Nordic As | Terapia de combinación para tratar cáncer con un poxvirus que expresa un antígeno de tumor y un anticuerpo monoclonal contra TIM-3 |
| CN106999577B (zh) | 2014-07-16 | 2021-12-21 | 特兰斯吉恩股份有限公司 | 溶瘤病毒和免疫检查点调节因子组合 |
| US20180028626A1 (en) | 2015-02-13 | 2018-02-01 | Transgene Sa | Immunotherapeutic vaccine and antibody combination therapy |
| CA2990549A1 (en) | 2015-07-31 | 2017-02-09 | Bavarian Nordic A/S | Promoters for enhancing expression in poxviruses |
| WO2017120204A2 (en) | 2016-01-05 | 2017-07-13 | The United States Of America, As Represented By The Secretary, Department Of Health And Human Services | Combination of histone deacetylase inhibitor and immunotherapy |
| US10822415B2 (en) | 2016-01-28 | 2020-11-03 | Inserm (Institut National De La Santéet De La Recherche Médicale) | Methods for enhancing the potency of the immune checkpoint inhibitors |
| WO2017136342A1 (en) | 2016-02-02 | 2017-08-10 | The United States Of America, As Represented By The Secretary, Department Of Health And Human Services | Fulvestrant for inducing immune-mediated cytotoxic lysis of cancer cells |
| US20190134190A1 (en) | 2016-05-04 | 2019-05-09 | Transgene Sa | Combination therapy with cpg tlr9 ligand |
| AU2017336269B2 (en) | 2016-09-28 | 2022-06-16 | Bavarian Nordic A/S | Compositions and methods for enhancing the stability of transgenes in poxviruses |
| WO2018069316A2 (en) | 2016-10-10 | 2018-04-19 | Transgene Sa | Immunotherapeutic product and mdsc modulator combination therapy |
| WO2018091680A1 (en) | 2016-11-18 | 2018-05-24 | Transgene Sa | Cowpox-based oncolytic vectors |
| US20190330655A1 (en) | 2016-12-28 | 2019-10-31 | Transgene Sa | Oncolytic viruses and therapeutic molecules |
| US11203611B2 (en) | 2017-04-14 | 2021-12-21 | Tollnine, Inc. | Immunomodulating polynucleotides, antibody conjugates thereof, and methods of their use |
| US11306292B2 (en) | 2017-05-15 | 2022-04-19 | Janssen Vaccines & Prevention B.V. | Stable virus-containing composition |
| US11052147B2 (en) | 2017-05-15 | 2021-07-06 | Janssen Vaccines & Prevention B.V. | Stable virus-containing composition |
| WO2019020543A1 (en) | 2017-07-28 | 2019-01-31 | Transgene Sa | ONCOLYTIC VIRUSES EXPRESSING AGENTS TARGETING METABOLIC IMMUNE MODULATORS |
| BR112020003669A2 (pt) | 2017-08-24 | 2020-09-01 | Bavarian Nordic A/S | terapia de combinação para tratamento do câncer com administração intravenosa de um mva recombinante e de um anticorpo |
| CA3093093A1 (en) | 2018-03-07 | 2019-09-12 | Transgene | Parapoxvirus vectors |
| WO2020011754A1 (en) | 2018-07-09 | 2020-01-16 | Transgene | Chimeric vaccinia viruses |
| WO2020049151A1 (en) | 2018-09-06 | 2020-03-12 | Bavarian Nordic A/S | Storage improved poxvirus compositions |
| US20220233607A1 (en) | 2019-05-29 | 2022-07-28 | Université De Tours | Toxoplasma platform for treating cancer |
| KR20220162130A (ko) | 2020-02-28 | 2022-12-07 | 탈락 테라퓨틱스, 인크. | 트랜스글루타미나제-매개된 접합 |
| IL296250A (en) | 2020-03-12 | 2022-11-01 | Bavarian Nordic As | Compositions improving poxvirus stability |
| CA3261778A1 (en) | 2022-08-18 | 2024-02-22 | Transgene | CHIMERIC POXVIRUSES |
Family Cites Families (17)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5174993A (en) | 1981-12-24 | 1992-12-29 | Health Research Inc. | Recombinant avipox virus and immunological use thereof |
| US4603112A (en) | 1981-12-24 | 1986-07-29 | Health Research, Incorporated | Modified vaccinia virus |
| US5833975A (en) * | 1989-03-08 | 1998-11-10 | Virogenetics Corporation | Canarypox virus expressing cytokine and/or tumor-associated antigen DNA sequence |
| DK354487A (da) * | 1986-07-11 | 1988-01-12 | Noboru Yanaihara | Oncogen-relaterede peptider |
| US5266313A (en) | 1987-02-03 | 1993-11-30 | The United States Of America As Represented By The Department Of Health And Human Services | Raccoon poxvirus as a gene expression and vaccine vector for genes of rabies virus and other organisms |
| DE10399031I1 (de) | 1987-08-28 | 2004-01-29 | Health Research Inc | Rekombinante Viren. |
| CA1340203C (en) | 1987-09-16 | 1998-12-15 | Noboru Yanagida | Recombinant apivoxvirus |
| US5093258A (en) | 1988-08-26 | 1992-03-03 | Therion Biologics Corporation | Recombinant fowlpox virus and recombination vector |
| WO1991019803A1 (en) | 1990-06-19 | 1991-12-26 | Applied Biotechnology, Incorporated | Self assembled, defective, nonself-propagating viral particles |
| AU674492B2 (en) * | 1991-05-06 | 1997-01-02 | United States of America, as represented by the Department of Health and Human Services, The | Recombinant virus expressing carcinoembryonic antigen and methods of use thereof |
| US5445953A (en) | 1991-08-26 | 1995-08-29 | Immuno Aktiengesellschaft | Direct molecular cloning of a modified poxvirus genome |
| US5382425A (en) | 1992-01-13 | 1995-01-17 | Syntro Corporation | Recombinant swinepox virus |
| US5374149A (en) | 1993-01-13 | 1994-12-20 | Computower Technologies, Corp. | Vertical storage conveyor with symmetrical motor drive system |
| EP0789774A2 (en) * | 1994-10-03 | 1997-08-20 | THE GOVERNMENT OF THE UNITED STATES OF AMERICA, as represented by THE SECRETARY, DEPARTMENT OF HEALTH AND HUMAN SERVICES | Enhanced immune response by introduction of cytokine gene and/or costimulatory molecule b7 gene in a recombinant virus expressing system |
| EP0784483B1 (en) * | 1994-10-03 | 2000-11-29 | THE GOVERNMENT OF THE UNITED STATES OF AMERICA, as represented by THE SECRETARY, DEPARTMENT OF HEALTH AND HUMAN SERVICES | Composition comprising a recombinant virus expressing an antigen and a recombinant virus expressing an immunostimulatory molecule |
| US6001349A (en) * | 1995-02-22 | 1999-12-14 | Therion Biologics Corporation | Generation of human cytotoxic T-cells specific for carcinoma self-associated antigens and uses thereof |
| US6165460A (en) * | 1995-07-10 | 2000-12-26 | Therion Biologics Corporation | Generation of immune responses to prostate-specific antigen (PSA) |
-
1997
- 1997-07-15 EP EP97934142A patent/EP0954593A1/en not_active Withdrawn
- 1997-07-15 EP EP09010415A patent/EP2112225A1/en not_active Ceased
- 1997-07-15 AU AU37268/97A patent/AU731860B2/en not_active Ceased
- 1997-07-15 JP JP50884498A patent/JP4475683B2/ja not_active Expired - Lifetime
- 1997-07-15 CA CA002261989A patent/CA2261989C/en not_active Expired - Fee Related
- 1997-07-15 WO PCT/US1997/012203 patent/WO1998004727A1/en not_active Ceased
-
2002
- 2002-07-17 US US10/197,127 patent/US7410644B2/en not_active Expired - Fee Related
-
2009
- 2009-07-22 JP JP2009171254A patent/JP2009278997A/ja active Pending
Non-Patent Citations (1)
| Title |
|---|
| J.W. HODGE ET AL INT. V. CANCER (1995) 63 PAGES 231-7 * |
Also Published As
| Publication number | Publication date |
|---|---|
| AU3726897A (en) | 1998-02-20 |
| US20030003079A1 (en) | 2003-01-02 |
| EP2112225A1 (en) | 2009-10-28 |
| CA2261989A1 (en) | 1998-02-05 |
| JP2009278997A (ja) | 2009-12-03 |
| WO1998004727A1 (en) | 1998-02-05 |
| CA2261989C (en) | 2008-09-30 |
| JP2000516459A (ja) | 2000-12-12 |
| EP0954593A1 (en) | 1999-11-10 |
| US7410644B2 (en) | 2008-08-12 |
| JP4475683B2 (ja) | 2010-06-09 |
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