AU724451B2

AU724451B2 - Method for producing beer

Info

Publication number: AU724451B2
Application number: AU68916/98A
Authority: AU
Inventors: Peter J. Degen; Gilbert Moser; Dieter Pelz; Horst Randhahn; Volker Ribitsch; Walter Serro; Gerald Zanker
Original assignee: Pall Corp
Current assignee: Pall Corp
Priority date: 1997-04-08
Filing date: 1998-04-07
Publication date: 2000-09-21
Anticipated expiration: 2018-04-07
Also published as: AU6891698A; JP3538203B2; BR9814243A; CN1259882A; JP2001519715A; EP0973603A1; KR20010006206A; CN1137765C; CA2286267A1; KR100538790B1; WO1998045029A1

Description

WO 98/45029 PCTIUS98/06969 1 METHOD FOR PRODUCING BEER TECHNICAL FIELD OF THE INVENTION The present invention relates to a method of producing beer, particularly of filtering beer through a filtration medium and cleaning the filtration medium with enzymes such that it can be reused in beer filtration.

BACKGROUND OF THE INVENTION In view of the extended marketing channels, germs bacteria) have to be removed from the beer in order to make it storable. Nowadays, germ removal is mainly carried out by pasteurization of the beer. To this end, the beer is, for example, bottled or canned, and heated to a temperature of between 62 and 69 OC to kill the germs.

This pasteurization does, however, involve considerable energy consumption. It has the further disadvantage that the energy introduced can trigger chemical reactions which impair the product and are difficult to control. These reactions can, for example, adversely affect the flavor of the product ("pasteurized taste"), and there is also the danger that undesired substances will form. Pasteurization is, therefore, a relatively expensive germ removing method involving high energy expenditure and, consequently, having harmful effects on the environment as well as reducing the quality of the product.

Another known germ removing method is coldfiltration. Cold-filtered beer is available as so-called "draft beer" in, for example, the United States, Japan and Korea. This beer is prohibited in Europe because it contains technical enzymes.

These technical enzymes are present in the beer to counteract a drawback inherent in the cold-filtration method: early clogging of the filter. This clogging is due to deposits of substances to be filtered out of the WO 98/45029 PCT/US98/06969 2 beer on the upstream side of the filter, a membrane filter. The deposits are difficult or even impossible to remove from the filter and reduce the service life of the filter. This increases the cost of producing the beer as membrane filters are expensive.

To prolong the service life of the filter, the manufacturers of membrane filters recommend cleaning the used membranes by treating them with proteases, glucanases, and xylanases, as well as with chemicals such as surfactants, acids/bases, and oxidizing agents, to make them reusable. This cleaning can be carried out at, for example, two stages, with the above-mentioned enzymes at a first stage, followed optionally by additional cleaning with the above-mentioned chemicals in a second stage.

The literature also discloses methods of cleaning membrane filters used in filtering beer, which cleaning methods involve a variety of techniques. For example, U.S. Patent 5,227,819 discloses a method for the cleaning of a polyamide microporous membrane used in coldfiltering beer by passing a dilute alkaline solution through the microporous membrane. International Patent Application WO 96/23579 discloses a somewhat different method of cleaning a membrane filter used in beer filtration. That method is characterized by treating the membrane filter with an enzyme-containing aqueous solution of P-glucanases, xylanases, and cellulases, cleaning the membrane filter with an acidic aqueous cleaning solution, and cleaning the membrane filter with a peroxide-containing alkaline cleaning solution.

Given, for example, a filter area of approximately 320 m 2 a cleaning procedure will, by way of example, make provision for enzymatic cleaning after every 5,000 hectoliters filtered and an additional chemical cleaning after every 20,000 hectoliters filtered. The typical service life of filters with the above-mentioned filter area of approximately 320 m 2 having undergone the WO 98/45029 PCT/US98/06969 3 manufacturer-recommended cleaning is approximately 100,000 hectoliters.

The previously known cleaning procedures do, however, have the disadvantage that they are unable to remove the deposits on the filter to a satisfactory extent, which causes the cleaning efficiency to diminish strongly as the membrane filter increases in age.

Yet another disadvantage is the sudden, random clogging of the filter membrane, unrelated to standard norms like total nitrogen content, or percent of original wort. A fully clogged membrane filter cannot be satisfactorily cleaned under procedures following the current state of technology, which greatly reduces the service life of the filter. It is difficult to determine when a filter will become so clogged that it cannot be satisfactorily cleaned, and, therefore, a filter may be cleaned prematurely or not in time, too early or too late.

In view of the foregoing problems, there exists a need for an improved method of producing beer, particularly wherein the beer can be filtered through a filtration medium that can be satisfactorily cleaned and reused. The present invention provides such a method.

These and other advantages of the present invention, as well as additional inventive features, will be apparent from the description of the invention provided herein.

BRIEF SUMMARY OF THE INVENTION The present invention provides a method for producing beer comprising filtering beer through a porous membrane until such time that the porous membrane is in need of cleaning, contacting the porous membrane with an enzyme selected from the group consisting of cellulases, amylases, and combinations thereof, particularly a cellulase having a crystalline:soluble cellulose activity ratio at 60 minutes of at least about 0.1, to clean the porous membrane, and then reusing the porous membrane to WO 98/45029 PCT/US98/06969 4 continue filtering beer. The present invention further provides a method for producing beer comprising filtering beer through a porous membrane that progressively clogs during filtration, monitoring the streaming or zeta potential of the porous membrane as a measure of the extent of clogging of the porous membrane, halting filtration of the beer through the porous membrane before the porous membrane becomes fully clogged as determined by the streaming or zeta potential of the porous membrane, cleaning the porous membrane, and then reusing the porous membrane to continue filtering beer.

BRIEF DESCRIPTION OF THE DRAWINGS Figure 1 is a graph of beer filtration amount (g) versus filtration time (sec) in connection with filtering beer through a previously unused, a new, porous membrane.

Figure 2 is a graph of beer filtration amount (g) versus filtration time (sec) in connection with filtering beer through a clogged porous membrane.

Figure 3 is a graph of beer filtration amount (g) versus filtration time (sec) in connection with filtering beer through a previously clogged porous membrane cleaned in accordance with a prior art technique.

Figure 4 is a graph of beer filtration amount (g) versus filtration time (sec) in connection with filtering beer through a previously clogged porous membrane cleaned in accordance with the present invention.

Figure 5 is a schematic diagram depicting a device for measuring the zeta potential of a filtration medium.

Figure 6 is a graph of filtration medium zeta potential (mY) versus electrolyte solution pH, wherein curve is for a new porous membrane, curve is for a porous membrane that has been partially clogged in connection with filtering beer, and curve is for a porous membrane that has been nearly fully clogged in connection with filtering beer.

WO 98/45029 PCT/US98/06969 Figure 7 is a schematic diagram depicting an apparatus for filtering beer using a bypass system and the measuring device of Fig. DESCRIPTION OF THE PREFERRED EMBODIMENTS The present invention provides a method for producing beer, preferably cold-filtered beer. The method comprises filtering beer through a porous membrane, a membrane filter, until such time that the porous membrane is in need of cleaning, contacting the porous membrane with an enzyme to clean the porous membrane, and then reusing the porous membrane to continue filtering beer.

It surprisingly has been discovered that porous membranes can be cleaned better and more gently with a cellulase and/or with an amylase than with proteases, xylanases, and/or glucanases. Cleaning in accordance with the present invention results in a considerable increase in the service life of porous membranes used in the filtering of beer and therefore greatly improves the commercial benefit attendant the use of porous membranes in the production of beer.

The enzyme is selected from the group consisting of cellulases, amylases, and combinations thereof. As indicated above, proteases, xylanases, and/or glucanases need not be used, and, preferably, are not used, with the cellulase and/or amylase to clean the porous membrane.

The cellulase desirably has a crystalline:soluble cellulose activity ratio (described more fully below) of at least about 0.1, more desirably at least about 0.3, preferably at least about 0.4, more preferably at least about 0.5, and most preferably at least about 1, particularly at least about 1.2. Suitable cellulases include cellulases derived from Aspergillus, particularly Aspergillus niger. Preferred cellulases include cellulases derived from Trichoderma, preferably Trichoderma reesei and Trichoderma longibrachiatum, and WO 98/45029 PCT/US98/06969 6 Thermomonospora, preferably from Thermomonospora fusca.

Other sources of cellulases are recited in U.S. Patent 4,912,056. Suitable amylases include a-amylase, pamylase, and combinations thereof. More preferably, no enzymes other than cellulases and amylases are utilized in the present inventive method, the porous membrane is not contacted with an enzyme other than a cellulase or an amylase. Most preferably, the enzyme utilized in the present inventive method is a cellulase, and optimally no enzyme other than a cellulase is utilized, the porous membrane is contacted with a cellulase and is not contacted with any other enzyme.

The porous membrane can be any membrane suitable for the filtration of beer. In the context of the present invention, the porous membrane typically will be a microporous membrane, a porous membrane with a pore rating of about 0.02-1 um. The porous membrane preferably will have a pore rating of about 0.1-1 [Im, most preferably about 0.45 pm. Such a porous membrane can be used to remove bacteria and other undesirable germs from the beer, preferably obviating the need to pasteurize the beer. The porous membrane also can be used to remove yeast and other undesirable substances from the beer. Suitable porous membranes include those prepared from inorganic materials such as ceramics and metals, as well as, preferably, organic polymers such as polyamides, polyethersulfones, polyolefins, polyvinylidenefluoride, and the like. The porous membrane preferably is a polyamide porous membrane, especially a nylon-6,6 porous membrane.

A preferred embodiment of the method according to the present invention is characterized in that the porous membrane is additionally brought into contact with an aqueous base, with the porous membrane being advantageously brought into contact with the aqueous base at a first stage and with the enzyme at a second stage.

The use of an aqueous solution of NaOH and/or KOH as the 7 aqueous base has proven expedient. It is preferable for the base to be present in a concentration of 0.1 to 1 N, more preferably 0.25 to 1 N, and most preferably 0.5 to 1 N. The treatment with the aqueous base is best carried out at a temperature of between 40 and 90 0C.

Further advantageous embodiments of the method according to the present invention are characterized in that the treatment with the cellulase is carried out at a temperature of between 40 and 50 IC and a pH of between 4.5 and 5.5, the treatment with the a-amylase is carried out at a temperature of between 60 and 75 OC and a pH of between 4.6 and 5.8, and the treatment with the P-amylase is carried out at a temperature of between 40 and 60 OC and a pH of between 4.6 and 5.8.

It can be expedient for the cleaning to be carried out until a point in time at which there is no more change in the streaming potential or the zeta potential of the porous membrane. It has been discovered that the 20 streaming potential occurring at the porous membrane 20 during operation or the zeta potential calculated from it (see below) is a good indication of the extent to which the substances clogging the porous membrane have been removed.

The present invention also aims at increasing the porous membrane's service life by ensuring that it is cleaned at a desirable time. Thus, the present invention provides for the production of beer comprising filtering beer through a porous membrane, which will clog progressively as filtration proceeds. Filtration is 30 halted at a given point when the porous membrane is only partially clogged, has not yet reached the condition of being totally clogged. The degree of clogging can be determined by any suitable means, desirably by monitoring the pressure drop across the porous membrane such as is generally described in U.S.

Patent 5,449,465. Alternatively, the present invention provides for an identification of the time for cleaning by determination of the streaming potential through the filter and/or zeta potential of the filter.

This aspect of the present invention is founded on the recognition that streaming potential or zeta potential extrapolated from the former's recorded data will change in a pH range (within which beer brewing or filtering occurs) according to the degree of clogging and thus represents a reliable, and almost quantitative, indicator of the state of clogging. Determination of the streaming potential and/or zeta potential of the porous membrane can hence give an accurate picture of a particular state of clogging.

Porous membranes are known to act in a two-fold way.

First, a porous membrane acts as a sieve, when particles larger then the filter's pores are mechanically filtered out of the medium. Secondarily, a porous membrane also **is known to act by electrostatic attraction. Particles of a diameter much smaller than the pore size of the p membrane are deposited thereon when the zeta potential of 20 the filter medium and that of the particles are of opposite polarity (see, Informational Brochure SD G of Pall Filtrationstechnik GmbH, Germany) Yet, not known prior to the present invention is the fact that zeta potential can be used to determine a porous membrane's degree of clogging.

A porous membrane's zeta potential will be affected by its chemical properties. One of ordinary skill in the art will have no difficulty being cognizant of the present invention 3 to select only filters whose zeta potential will change 30 at a great enough rate relative to the degree of clogging. With the filter on-line, and by way of continuous monitoring through data acquisition, the filtration process can be halted at an appropriate time, once clogging sets in.

The cleaning of a filter not yet fully clogged is much easier, while assuring longer service life, than the cleaning of a totally clogged filter. Thus, a preferred method of the present invention has filtration halted at a point when the filter's zeta potential has decreased to a maximum of 20% of the value it exhibited in its unused state, or when clogging does not exceed Another refinement of the process will use a porous membrane of polyamide, with filtration halted when the zeta potential exceeds -5 mV as measured at a pH of 4.2.

The beer preferably will undergr- pre-filtration before filtration proper, filtration through the porous membrane. Diatomateous (or infusorial) earth, also known as diatomite, is almost exclusively used for pre-filtration. A combination of diatomateous earth and deep-bed filtration also is feasible.

The present invention can be used in any suitable beer production system. Preferably, the present invention is used in connection with the cluster filter system as described in U.S. Patents 5,417,101 and 5,594,161.

The present invention also relates to a filtration unit for filtering beer, with a feeder line for the filtration-bound beer, a porous membrane, and a run-off line for the filtered beer. It is characterized by a module in the form of a meter cell, functioning as bypass, and featuring a porous membrane and means, e.g., electrodes, for monitoring the streaming potential and/or zeta potential of the meter cell's membrane filter through which beer flows.

The present invention also deals with a filtration unit for filtering beer, with the unit featuring a feeder line for filtration-bound beer, a porous membrane, and a run-off line for filtered beer. In divergence from the foregoing paragraph, the filtrationunit is characterized by means, electrodes, being attached to the porous membrane for monitoring or reading the streaming potential and/or zeta potential as the beer flows through the porous membrane. In this variation, the zeta potential is not measured via the meter cell assigned as AMENDED SHEET WO 98/45029 PCT/US98/06969 bypass to the membrane filter, but rather on the membrane filter itself.

Any suitable bypass configuration can be utilized in connection with the embodiments of the present invention.

Preferably, the present invention incorporates the apparatus and method described in U.S. Patent 5,449,465.

The discovery that the filter's zeta potential correlates to the general state of clogging can be implemented in beer filtration as follows: 1. Through constant observation of changes taking place in the streaming potential and/or zeta potential of the porous membrane during the filtration process, the membrane's degree of clogging can be pinpointed in order to prevent an unexpected or random occurrence, while timely measures for an exchange of filters can be taken.

2. Filtration can be halted before the porous membrane becomes totally clogged. This promotes easier cleaning of the filter. It has been shown that the clogging substances in a totally clogged filter can only be removed with the greatest of difficulty by conventional methods of cleansing, or cannot be removed from the filter at all, resulting in abbreviated service life.

Once filtration is halted prior to total clogging, the process of cleaning is much easier and more thorough, with the filter retaining an extended life. In the instance of a polyamide porous membrane, it has been discovered that the successful removal of all clogging substances from the porous membrane can be accomplished when filtration is halted at a point where the zeta potential has not lost more than about 80% of its original value, is not clogged in excess of 3. The cleaning method's success can be tested by determining the cleaned membrane's zeta potential. The act of cleaning will return the zeta potential to approximately its original value.

11 By this procedure, the cleaning process can be evaluated and/or optimized for it's efficiency: 4. The aging of a porous membrane for reasons of.

repeated use can be tracked, providing a handy estimate as to its remaining service life expectancy.

By measuring zeta potential, filter material and shunting materials diatomite, bentonite, perlite, polyvinyl pyrrolidone) can be tested for suitability in beer filtration by assessing the interaction between clogging substances of liquid systems and filter material and/or shunting means for filters.

6. The service life of a porous membrane can be estimated by way of measuring zeta potential, whereunder a specific membrane load (hl/mn) is recorded up to the point when clogging sets in.

The artisan is aware that most suitable for the process are porous membranes with a zeta potential exhibiting pronounced change in relation to the degree of 2 clogging. Verification of these parameters is easy enough by employing the aforementioned simple test method.

S S The following examples further illustrate the present invention but, of course, should not be construed as in 25 any way limiting its scope.

*SSS

:EXAMPLE 1 This example illustrates the effectiveness of the present inventive method to produce beer. In particular, 30 this example demonstrates that cellulases and amylases can be used to satisfactorily clean a porous membrane clogged in the course of beer filtration such that the porous membrane can be reused in continued beer filtration.

A porous membrane made of nylon-6,6 (NB type, commercially available from Pall Filtrationstechnik GmbH, Germany) was used as a filter. Such a filter is frequently used in the state of the art for the cold-filtration of beer.

12 The so-called membrane filter test according to Esser (Monatszeitschrift fur Brauerei (Monthly Magazine for Breweries), 25 th year, No. 6, pages 145-151, 1972) was used to determine the filtering performance of the filter. This test is reliable for checking measures for improving filterability.

To determine the filtering efficiency of a new, unused, porous membrane, a pressure filtration apparatus (SM 16526 type, 200 ml capacity; manufacturer: Sartorius GmbH, Goettingen, Germany) was used for a polyamide nylon-6,6 porous membrane having a 47 mm diameter and a 0.2 im pore size.

Beer cooled down to 0 oC was passed through the porous membrane under isobaric conditions (1 bar), and the amount of filtrate was weighed every 10 seconds. The test was stopped after 200 g of filtrate were obtained.

The result is shown as a graph in the diagram of Figure 1. Figure 1 shows that, under the conditions indicated above, the 200 g of filtrate were obtained with the 20 unused filter after approximately 210 seconds.

Under identical conditions, the filtering performance of a partially clogged, used, porous membrane was tested. The result is given in Figure 2 which shows that *..:even in 720 seconds only approximately 60 g of filtrate e 25 were obtained.

The clogged porous membrane was cleaned in accordance with a prior art method, wherein the membrane was first cleaned enzymatically and then chemically, as described below.

For enzymatic cleaning, the clogged membrane was treated for 1 hour with a 1% aqueous solution of a mixture of P-glucanases and xylanases (P3-Ultrasil manufacturer: Henkel) with a pH of 5(adjusted with a 0.05% aqueous solution of a mixture of surfactants and an acidic component (P3-Ultrasil 75; manufacturer: Henkel)) 'i at a temperature of 50 o C. This treatment was subsequently carried out one more time.

The membrane was then treated for 3 hours with a aqueous solution of a mixture of surfactants, glucanases, and proteases (P3-Ultrasil 62; manufacturer: Henkel) with a pH of 9-9.5 (set with a 0.15% aqueous solution of a mixture of surfactants and an alkaline component (P3-Ultrasil 91; manufacturer: Henkel)) at a temperature of 50 °C and subsequently rinsed with warm water (50 OC) For chemical cleaning, the membrane was thereafter treated for 30 minutes with a 1% aqueous solution of a mixture of surfactants and an acidic component (P3-Ultrasil manufacturer: Henkel) at 60 OC, and then rinsed with fresh water. The membrane was subsequently treated for minutes with an aqueous solution containing 1% of a mixture of surfactants and an alkaline component (P3- Ultrasil 91; manufacturer: Henkel) and 1% of a mixture of surfactants and an oxygen donor (P3-Ultrasil manufacturer: Henkel) at a temperature of 60 oC and then rinsed with fresh water. The membrane was then treated 20 once more for 30 minutes with a 0.5% aqueous solution of a mixture of surfactants and an acidic component (P3- Ultrasil 75; manufacturer: Henkel) and subsequently rinsed with fresh water until the rinse water reached the electrical conductivity of fresh water.

25 The filtering performance of this cleaned porous membrane was then tested again under the conditions indicated above. The result is shown in Figure 3.

Figure 3 shows that the filtering performance has improved somewhat as the 200 g of filtrate were obtained 30 after approximately 600 seconds.

A similarly clogged membrane whose filtering efficiency is shown in Figure 2 was cleaned in accordance with the method according to the present invention. The membrane was treated for 30 minutes with an aqueous solution of C and Cx-cellulases, the solution having a pH value of 4.7, at a temperature of 45 OC. The membrane was then treated Swith the same solution, but at a pH value of 5.0 and a WO 98/45029 PCTIUS98/06969 14 temperature of 50 and, finally, at a pH value of 4.7 and a temperature of 60 OC for 60 minutes.

The membrane was subsequently rinsed with warm water at 50 OC. The filtering performance of the membrane cleaned in accordance with the present invention was tested in accordance with the above procedure. The result is shown in Figure 4.

Figure 4 shows that 200 g of filtrate were obtained after approximately 220 seconds. This represents a significant improvement over the prior art (Figure 3) The method according to the present invention, therefore, allows considerably better cleaning of a used membrane filter than is possible with prior art cleaning methods.

Equally good results were obtained when, in accordance with the present invention, an amylase was used instead of a cellulase. The service life of a porous membrane thus can be increased with the cleaning method according to the present invention.

EXAMPLE 2 This example illustrates the use of the streaming or zeta potential of a porous membrane to assist in the cleaning of the porous membrane. In particular, the streaming or zeta potential is demonstrated to be useful in determining the extent of membrane cleaning as well as when a membrane is most satisfactorily cleaned.

The zeta potential of membrane filters was determined with the electrokinetic measuring system EKA of Anton Paar GmbH, Austria. This measurement is based on the streaming potential method. An electrolyte flows through the filters, and the potential (streaming potential) which is produced by shearing-off of counterions is detected with electrodes, and the zeta potential is calculated from this measured quantity (see below) Figure 5 shows diagrammatically the measuring cell with which the streaming potential or the zeta potential was determined. Reference numeral 1 designates the measuring cell in which the porous membrane 2 is clamped without warping in filter holders 3 and 4 made of polytetrafluoroethylene. The filter holders 3 and 4 are the end pieces of two pistons 5 and 6, respectively, which are mounted for displacement in the cylindrical part 7 of the measuring cell i.

The end pieces 3 and 4 of the pistons 5 and 6, respectively, have fine bores 10 and 11 for the fluid which is to be filtered and press the perforated electrodes 8 and 9 against the porous membrane 2. The electrodes 8 and 9 are connected to the two electric terminals 12 and 13 extending inside the pistons 5 and 6 so the streaming potential built up as fluid flows through the membrane 2 can be measured. Silver electrodes or silver chloride electrodes which exhibit a low polarization during passage of current are preferred for the electrodes. The pistons 6 and 7 are mounted in the seals 14 and 15, respectively, such that, on the one hand, they are displaceable, and, on the other hand, they do not allow any fluid to leak from the measuring cell.

The fluid to be filtered flows through the supply line 16 into the cylindrical part 7 of the measuring cell 1, through the fine bores 10 of the piston 6, through the electrode 8, with an electric potential being built up, and through the porous membrane 2. The filtered fluid flows through the electrode 9, with a potential likewise being built up, passes through the fine bores 11 of the piston and leaves the measuring cell through the discharge line 17.

To determine the zeta potential from the measured streaming potential, measurement (not illustrated) of the S differential pressure in the measuring cell between supply line 16 and discharge line 17, the conductivity and also the pH value is necessary. The zeta potential is calculated from these measured quantities as follows: AMENDED SHEET U LF.n zeta potential Ap S. o where U is the streaming potential, Ap the pressure difference, LF the conductivity, r1 the viscosity, and Es.

the dielectric constant.

The change in the zeta potentie] of the membrane filter as clogging progresses is shown in Figure 6. This figure is a diagram in which the zeta potential in millivolts is plotted as ordinate, and the pH value at which the zeta potential was determined as abscissa. The pH value of the electrolyte solution (0.001 N aqueous KCl solution) was set with 0.1 N HCl or with 0.1 N NaOH. The specified pressure difference was 350 mbar.

The diagram was obtained by first determining with the measuring cell described above the zeta potentials of a new, unused porous membrane made of polyamide (NB type, commercially available from Pall Filtrationstechnik GmbH, 6072 Dreieich 1, Germany) at various pH values.

The results relating to the unused porous membrane are plotted as curve It is evident that the unused porous membrane has a zeta potential of approximately -18 mV with an alkaline pH, and that the zeta potential increases with decreasing pH and finally reaches zero value at a pH of approximately 3.

Curve shows the dependence of the zeta potential on the pH value of the porous membrane under identical measuring conditions, as stated above, but after use thereof for filtering beer and, therefore, with partial clogging. As is apparent, the zetalpotential is raised somewhat by the partial clogging anid only reaches a value of approximately -15 mV at pH values of approximately 7.

Curve was plotted for the same porous membrane in the nearly fully clogged state. It is evident that the zeta potential now changes only slightly with the pH value, and even in the alkaline range does not fall below approximately -2 mV.

AMENDED SHEET WO 98/45029 PCTIUS98/06969 17 To test the cleaning according to the present invention, the zeta potential of the membrane to be cleaned is determined, and the cleaning was successful if the zeta potential of the cleaned membrane shifted as far as possible in the direction of the zeta potential of the unused membrane.

It will be clear to one skilled in the art that porous membranes whose zeta potential changes to a sufficiently great extent as a function of the degree of clogging are particularly well-suited for use in the method according to the present invention. This characteristic can be easily determined by one skilled in the art by simple testing.

A porous membrane of polyamide is especially suitable in the context of the process since the zeta potential at the pH of the filtration-bound beer (ca. pH 4.2) will undergo severe change with progressive clogging. As can be learned from Figure 6, the membrane at this particular pH value at the beginning of filtration shows a zeta potential of approximately -8 mV.

The totally clogged membrane has a zeta potential of approximately -2 mV.

Figure 7 shows a variation of the discussed filtration unit featuring a filtration chamber 18, with a meter cell 22 assigned to it as a bypass, as depicted in Figure 5. The filtration chamber 18 holds filter candles 19.

The filtration-bound beer is fed via line 20 into the filtration chamber 18, flowing through the filter candles (membrane filter) 19, and exits the filter chamber 18 through run-off line 21 in the form of filtered beer.

The meter cell is shown in Figure 7 without detail.

The actual flow through the meter cell 22 must be controlled to the extent that an amount of beer is filtered per cm 2 of the porous membrane's surface which is 18 equal to the amount of porous membrane surface per cm 2 in the filtration chamber 18.

The severe change in zeta 'otential of the filter membrane 2 (Figure 5) zeta potential inside meter cell 1 during filtration allows an assessment of the state of the filter candles 19 in filtration chamber 18.

EXAMPLE 3 This example illustrates the effectiveness of cellulase derived from Aspergillus niger in enzymatically degrading soluble and crystalline cellulose substrates.

Cellulase derived from Aspergillus niger was obtained from Fluka (item numbers 22178). The enzyme was evaluated with respect to two different celluloses: soluble carboxymethylcellulose (CMC, available from Aldrich as item number 41927-3) and crystalline cellulose (Avicel, available from FMC as item number PH-105).

The test methodology involved the preparation of an incubation solution of 18 ml CMC or Avicel 20 (ii) 5 ml sodium acetate buffer (50 mM, pH and (iii) 5 ml of a solution of the enzyme in sodium acetate buffer (50 mM, pH 4.8) at 30 OC. A test solution then was prepared by mixing 1.4 ml of the incubation solution with 0.1 ml glucose solution and 1.5 ml 25 dinitrosalicylic acid (DNS) reagent (available from Sigma as item number D-0550). The test solution was boiled for 15 minutes. The total mol glucose equivalents/mg enzyme as a function of time (min) was determined spectroscopically (575 nm), using two parallel samples, in accordance with the procedure described in Miller, Anal.

Chem., 31, 426-28 ('1959), using a straight line calibration with a glucose standard. Protein amounts were determined in accordance with the procedure described in Bradford, Anal. Biochem., 72, 248-64 (1976), using a bovine serum albumin (BSA) standard.

The enzymatic degradation of cellulose results in the S production of glucose, and, therefore, the measurement of WO 98/45029 PCT/US98/06969 19 pmol glucose equivalents/mg enzyme is a measure of the activity of the enzyme with respect to a particular type of cellulose, soluble (CMC) or crystalline (Avicel) cellulose.

.The results of this evaluation with respect to the cellulase derived from Aspergillus niger are set forth in Table 1. The test solution with the soluble (CMC) cellulose substrate contained 0.8 mg enzyme/28 ml incubation solution (ca. 17.6 [pg protein). The test solution with the crystalline (Avicel) cellulose substrate contained 0.35 mg enzyme/28 ml incubation solution (ca.

7.7 (ig protein).

Table 1: Cellulase derived from Aspergillus niger Time (min) Glucose Equivalents (imol/mg enzyme) Soluble Crystalline Crystalline: Cellulose Cellulose Soluble Substrate Substrate Cellulose Activity Ratio 0 0 0 27.0 0 0 28.5 1.7 0.06 30.5 34.0 1.7 0.05 34.8 4.0 0.11 37.5 3.5 0.09 37.8 3.7 0.10 105 38.3 120 39.5 10.3 0.26 Those enzymes that have a relatively greater activity toward crystalline cellulose substrates as compared to soluble cellulose substrates have been found to be particularly effective in cleaning porous membranes used in beer filtration. The ratio of the glucose equivalents produced with respect to the crystalline cellulose substrate and the glucose equivalents produced with WO 98/45029 PCT/US98/06969 respect to the soluble cellulose substrate thus is an indicator of the effectiveness of the enzyme in the context of the present invention and is described as the crystalline:soluble cellulose activity ratio. Desirably, the crystalline:soluble cellulose activity ratio has the previously described values at a range of times in the test protocol described in this example, at minutes, 60 minutes, and/or 90 minutes, especially at minutes.

As is apparent from the data set forth in Table 1, the cellulase from Aspergillus niger has a crystalline:soluble cellulose activity ratio at 60 minutes of 0.11, indicating that it is a moderately effective enzyme for purposes of cleaning porous membranes used in connection with the filtration of beer.

EXAMPLE 4 This example illustrates the effectiveness of cellulase derived from Trichoderma reesei in enzymatically degrading soluble and crystalline cellulose substrates.

Cellulase derived from Trichoderma reesei was obtained from Fluka (item numbers 22173). The enzyme was evaluated in the same manner as recited in Example 3.

The results of this evaluation with respect to the cellulase derived from Trichoderma reesei are set forth in Table 2. The test solution with the soluble (CMC) cellulose substrate contained 0.37 mg enzyme/28 ml incubation solution (ca. 128 pg protein). The test solution with the crystalline (Avicel) cellulose substrate contained 0.08 mg enzyme/28 ml incubation solution (ca.

25.6 pg protein).

WO 98/45029 PCT/US98/06969 Table 2: Cellulase derived from Trichoderma reesei Time (min) Glucose Equivalents (tmol/mg enzyme) Soluble Crystalline Crystalline: Cellulose Cellulose Soluble Substrate Substrate Cellulose Activity Ratio 0 0 0 62.6 0 84.7 21.5 0.25 96.3 30.0 0.31 99.5 40.0 0.40 152.0 57.5 0.38 139.0 75.0 0.54 178.4 85.0 0.48 184.2 95.0 0.52 105 172.6 100.0 0.58 120 193.7 115.0 0.59 As is apparent from the data set forth in Table 2, the cellulase from Trichoderma reesei has a crystalline:soluble cellulose activity ratio at 60 minutes of 0.54, indicating that it is a superior enzyme for purposes of cleaning porous membranes used in connection with the filtration of beer.

EXAMPLE This example illustrates the effectiveness of Pcellulase derived from Bacillus subtilis in enzymatically degrading soluble and crystalline cellulose substrates.

P-cellulase derived from Bacillus subtilis was obtained from Fluka (item numbers 49106). The enzyme was evaluated in the same manner as recited in Example 3.

The results of this evaluation with respect to the 3cellulase derived from Bacillus subtilis are set forth in Table 3. The test solution with the soluble (CMC) cellulose substrate contained 14.4 mg enzyme/28 ml incubation solution (ca. 8.3 Jg protein). The test WO 98/45029 PCT/US98/06969 22 solution with the crystalline (Avicel) cellulose substrate contained 15.6 mg enzyme/28 ml incubation solution (ca.

8.8 tg protein).

Table 3: -Cellulase derived from Bacillus subtilis Time (min) Glucose Equivalents (Vmol/mg enzyme) Soluble Crystalline Crystalline: Cellulose Cellulose Soluble Substrate Substrate Cellulose Activity Ratio 0 0 0 1.1 0.1 0.09 1.0 0.1 0.10 0.9 0.1 0.11 0.9 0.1 0.11 1.0 0.1 0.10 1.0 0.1 0.10 1.0 0.2 0.20 1.1 0.2 0.18 105 1.1 0.1 0.09 120 1.3 0.1 0.08 As is apparent from the data set forth in Table 3, the P-cellulase from Bacillus subtilis has a crystalline:soluble cellulose activity ratio at 60 minutes of 0.10, indicating that it is a moderately effective enzyme for purposes of cleaning porous membranes used in connection with the filtration of beer.

EXAMPLE 6 This example illustrates the effectiveness of exocellulase derived from Thermomonospora fusca in enzymatically degrading soluble and crystalline cellulose substrates.

Exocellulase E3 derived from Thermomonospora fusca was obtained from Cornell University. The enzyme was evaluated in the same manner as recited in Example 3 except that the incubation solution comprised 18 ml CMC or Avicel (ii) 9 ml sodium acetate buffer mM, pH and (iii) 1 ml of a solution of the enzyme in sodium acetate buffer (50 mM, pH shaken at 50 °C (ca. 960 uIm protein). The test solution was evaluated using a color test rather than the DNS test recited in Example 3.

The results of this evaluation with respect to the exocellulase derived from Thermomonospora fusca are set forth in Table 4.

Table 4: Exocellulase derived from Thermomonospora fusca Time (min) Glucose Equivalents (4mol/mg enzyme) Soluble Crystalline Crystalline:.

Cellulose Cellulose Soluble Substrate Substrate Cellulose SActivity Ratio 0 0 0 0.1 0 0.1 0.3 3.00 0.2 0.3 1.50 0.2 0.3 1.50 45 0.3 0.4 1.33 0.3 0.4 1.33 75 0.3 0.5 1.67 0.3 0.3 1.00 As is apparent from the data set forth in Table 4, the exocellulase frcm Thermomnonospora fusca has a crystalline:soluble cellulose activity ratio at 60 minutes of 1.33, indicating that it is a superior enzyme for purposes of cleaning porous membranes used in connection with the filtration of beer.

WO 98/45029 PCTIUS98/06969 24 EXAMPLE 7 This example illustrates the effectiveness of aamylase derived from Bacillus subtilis in enzymatically degrading soluble and crystalline cellulose substrates.

a-amylase derived from Bacillus subtilis was obtained from Fluka (item numbers 10069). The enzyme was evaluated in the same manner as recited in Example 3 except that the incubation solution comprised 18 ml CMC or Avicel (ii) 5 ml sodium acetate buffer (50 mM, pH and (iii) 5 ml of a solution of the enzyme in sodium acetate buffer (50 mM, pH shaken at 30 0 C (ca. 8.5 tm protein). The test solution was evaluated using a color test rather than the DNS test recited in Example 3.

The results of this evaluation with respect to the aamylase derived from Bacillus subtilis are set forth in Table Table 5: a-Amylase derived from Bacillus subtilis Time (min) Glucose Equivalents (pmol/mg enzyme) Soluble Crystalline Crystalline: Cellulose Cellulose Soluble Substrate Substrate Cellulose Activity Ratio 0 0 0 0.1 0 0 0.1 0 0 0.1 0 0 0.1 0 0 0.1 0 0 0.1 0 0 0.1 0 0 0 As is apparent from the data set forth in Table the a-amylase from Bacillus subtilis has a crystalline:soluble cellulose activity ratio at 60 minutes of about 0 pmol detection limit), indicating that it WO 98/45029 PCTIUS98/06969 is not as effective an enzyme for purposes of cleaning porous membranes used in connection with the filtration of beer as the previously described cellulases.

EXAMPLE 8 This example illustrates the effectiveness of various cellulases in enzymatically degrading soluble and crystalline cellulose substrates.

Cellulase preparations were obtained from the Erbsloh Company: Cx-cellulase (powder, item number VP 0945/2),

C

1 -cellulase from Trichoderma reesei (powder, item number VP 0965/2), C,-cellulase (liquid, item number Cleanzym SB1), C,-cellulase (liquid, item number VP 0976/4), cellulase (liquid, item number VP 0971/1), and cellulase (liquid, item number VP 0971/4). The enzymes were evaluated in a manner similar to that recited in Example 3 except that the incubation solutions comprised 23 ml CMC or Avicel in a sodium acetate buffer (50 mM, pH and (ii) 5 ml of a solution of the enzyme in sodium acetate buffer (50 mM, pH 0.5% stock solutions were prepared from the powdered enzyme preparations (5 mg/ml) and liquid enzyme preparations (5 pi/ml). The solutions were shaken at oC. The test solution was evaluated after making a dilution using a color test rather than the DNS test recited in Example 3.

The results of this evaluation with respect to the various cellulases are set forth in Table 6. The glucose equivalents data is in terms of average umol glucose equivalents/min (for the total time interval) and are not normalized per mg enzyme (as was the situation with the data recited in Tables The crystalline:soluble cellulose activity ratio, of course, is not altered by the units for the glucose equivalents inasmuch as the units divide out in calculating the ratio the ratio is unit-less).

Table 6: Cellulase Preparations Time (min) Glucose Equivalents imol/min) Preparation Preparation Preparation (c) Soluble Crystalline Crystalline: Soluble Crystalline Crystalline: Soluble crystalline Crystalline: Cellulose Cellulose Soluble Cellulose Cellulose Soluble Cellulose Cellulose Soluble Substrate Substrate Cellulose Substrate Substrate Cellulose Substrate Substrate Cellulose Activity Activity Activity Ratio Ratio Ratio 0 0 0 0 0 0 0 0.12 0 0 0.14 0.11 0.79 0.09 0.05 0.56 0.06 0.01 0.17 0.11 0.08 0.73 0.09 0.06 0.67 0.06 0.01 0.17 0.08 0.09 1.13 0.08 0.04 0.50 0.02 0.02 1.00 0.07 0.05 0.71 0.07 0.03 0.43 0.04 0.02 0.50 0.06 0.04 0.67 0.03 0.02 0.67 0.03 0.02 0.67 0.04 0.03 0.75 0.05 0.02 0.40 0.03 0.02 0.67 0.04 0.03 0.75 0.04 0.02 0.01 0.50 0.03 0.03 1.00 0.03 0.2 0.67 Time (min) Glucose Equivalents (jmol/min) _________Preparation Preparation Preparation (f) Soluble Crystalline Crystalline: Soluble Crystalline Crystalline: Soluble Crystalline Crystalline: Cellulose Cellulose Soluble Cellulose Cellulose Soluble Cellulose Cellulose Soluble Substrate Substrate Cellulose Substrate Substrate Cellulose Substrate Substrate Cellulose IActivity Activity Activity 1.Ratio Ratio Ratio 0 0.01 0.19 0.15 0.10 0.07 0.06 0.05 0.04 0 0.23 0 .14 0.12 0.09 0.07 0.06 0.06 0.06 23.0 0 .74 0.80 0.90 1.00 1.00 1.20 1.50 0 0.15 0.10 0 .06 0.05 0.04 0.03 0.03 0.03 0 0.13 0.09 0.05 0.03 0.03 0.02 0.02 0.02 0.87 0.90 0.83 0.60 0.75 0.67 0.67 0.67 0.07 0 .06 0 .04 0.03 0.02 0.02 0.02 0.01 0.04 0.03 0.02 0.01 0.01 0.01 0 .01 0.01 0.57 0.50 0.50 0.33 0 0 0.50 1 .00 WO 98/45029 PCTIS98/06969 27 As is apparent from the data set forth in Table 6, the various cellulases have crystalline:soluble cellulose activity ratios at 60 minutes ranging from 0.4-1.0, indicating that they are superior enzymes for the purpose of cleaning porous membranes used in connection with the filtration of beer.

EXAMPLE 9 This example further illustrates the effectiveness of the present inventive method to produce beer. In particular, this example demonstrates that cellulases alone without the use of other enzymes) are superior in the cleaning of porous membranes clogged in the course of beer filtration for the purpose of returning the porous membrane to use in continued beer filtration.

Beer of different characteristics was filtered through nylon-6,6 porous membranes (ca. 300 m 2 with a pore rating of 0.45 pm in a cluster filter arrangement (PALL-CFS, available from Pall Filtrationstechnik GmbH, Germany). At certain beer filtration intervals, the porous membranes were subjected to a cleaning process in accordance with the present invention.

The cleaning process involved circulation of a NaOH solution for 15 minutes, followed by a 60 minute soak. The porous membranes then were backflushed with water. An internal loop was established through the cluster filter arrangement with water at 38 Lactic acid was added to the water to adjust the pH to 4.2 0.3, and then 6 1 of an enzyme preparation containing a cellulase derived from Trichoderma longibrachiatum obtained from the Erbsloh Company (item number VP 0945/1) was added to the water via a dosing pump. The enzyme preparation in the water (at a concentration of about g enzyme/100 kg filter housing fluid volume) was circulated for about 15 minutes, followed by a 30 minute soak, another 15 minute circulation, and finally a 6 hour soak. The porous membranes then were backflushed with water.

The porous membranes were cleaned after about 90,000 hi total beer was filtered through the porous membranes, and then the porous membranes were returned to service, to continue filtering beer. The porous membranes similarly were cleaned and returned to service after about 100,000 hl, about 140,000 hl, and about 165,000 hi total beer was filtered through the porous membranes.

The porous membranes mechanically failed after about 190,000 hi total beer was filtered through the porous membranes.

The foregoing data demonstrates that beer can be satisfactorily produced using the present invention.

Specifically, the results of this example demonstrate that a porous membrane can be effectively cleaned and returned to service in accordance with the present invention, thereby prolonging the useful life of the "20 porous medium in a beer production process.

9.

All of the references cited herein, including patents, patent applications, and publications, are hereby 49e* incorporated in their entireties by reference.

25 While this invention has been described with an emphasis upon preferred embodiments, it will be obvious to i those of ordinary skill in the art that variations of the preferred embodiments may be used and that it is intended that the invention may be practiced otherwise than as specifically described herein. Accordingly, this invention includes all modifications encompassed within the spirit and scope of the invention as defined by the following claims.

Throughout the description and claims of this specification, the word "comprise" and variations of the word, such as "comprising" and "comprises" is not intended to exclude other additives, integers or process steps.

Claims

1. A method for producing beer comprising filtering beer through a porous membrane until such time that said porous membrane is in need of cleaning, contacting said porous membrane with an enzyme selected from the group consisting of cellulaz-s, amylases, and combinations thereof in the absence of a protease or a glucanase to clean said porous membrane, and then reusing said porous membrane to continue filtering beer.

2. The method of claim 1, wherein said porous membrane is not contacted with an enzyme other than said cellulase or said amylase.

3. The method of claim 1 or 2, wherein said porous membrane is contacted with said cellulase.

4. A method for producing beer comprising filtering beer through a porous membrane until such time that said porous membrane is in need of cleaning, contacting said porous membrane with a cellulase having a crystalline:soluble cellulose activity ratio at minutes of at least about 0.1 to clean said porous membrane, and then reusing said porous membrane to continue filtering beer. The method of claim 3 or 4, wherein said porous membrane is contacted with said cellulase and is not contacted with any other enzyme.

6. The method of any of claims 1-3 or 5, wherein said cellulase has a crystalline:scluble cellulose activity ratio at 60 minutes of at least about 0.1.

7. The method of claim 6, wherein said cellulase has a crystalline:soluble cellulose activity ratio at minutes of at least about 0.3.

8. The method of claim 7, wherein said cellulase has a crystalline:soluble cellulose activity ratio at RA4 minutes of at least about 0.4. 11 WI -o/ AMENDED SHEET WO 98/45029 PCTIUS98/06969

9. The method of claim 8, wherein said cellulase has a crystalline:soluble cellulose activity ratio at minutes of at least about The method of claim 9, wherein said cellulase has a crystalline:soluble cellulose activity ratio at minutes of at least about 1.

11. The method of claim 10, wherein said cellulase has a crystalline:soluble cellulose activity ratio at minutes of at least about 1.2.

12. The method of any of claims 1-11, wherein said cellulase is derived from Trichoderma.

13. The method of claim 12, wherein said Trichoderma is Trichoderma reesei or Trichoderma longibrachiatum.

14. The method of any of claims 1-11, wherein said cellulase is derived from Thermomonospora. The method of claim 14, wherein said Thermomonospora is Thermomonospora fusca.

16. The method of any of claims 1-3 and 6-15, wherein said porous membrane is contacted with said amylase.

17. The method of claim 16, wherein said amylase is selected from the group consisting of a-amylase, P- amylase, and the combination thereof.

18. The method of any of claims 1-17, wherein said porous membrane is additionally contacted with an aqueous base prior to being reused.

19. The method of claim 18, wherein said porous membrane is contacted with said aqueous base prior to being contacted with said enzyme. The method of claim 18 or 19, wherein said aqueous base is an aqueous solution of NaOH and/or KOH.

21. The method of any of claims 18-20, wherein said base is present in a concentration of 0.1-1 N in said aqueous base. WO 98/45029 PCT/US98/06969 31

22. The method of any of claims 18-21, wherein said porous membrane is contacted with said aqueous base at a temperature of 40-90 °C.

23. The method of any of claims 1-22, wherein said porous membrane is contacted with said cellulase at a temperature of 40-50 0C and a pH of 4.5-5.5.

24. The method of any of claims 1-3 and 6-23, wherein said porous membrane is contacted with a-amylase at a temperature of 60-75 0C and a pH of 4.6-5.8.

25. The method of any of claims 1-3 and 6-23, wherein said porous membrane is contacted with P-amylase at a temperature of 40-60 0C and a pH of 4.6-5.8.

26. The method of any of claims 1-25, wherein said porous membrane is cleaned until the zeta potential of said porous membrane ceases to change.

27. The method of any of claims 1-26, wherein said time that said porous membrane is in need of cleaning is determined by the pressure drop across said porous membrane.

28. The method of any of claims 1-26, wherein said time that said porous membrane is in need of cleaning is determined by the streaming or zeta potential of said porous membrane.

29. A method for producing beer comprising filtering beer through a porous membrane that progressively clogs during filtration, monitoring the streaming or zeta potential of said porous membrane as a measure of the extent of clogging of said porous membrane, halting filtration of the beer through said porous membrane before said porous membrane becomes fully clogged as determined by the streaming or zeta potential of said porous membrane, cleaning said porous membrane, and then reusing said porous membrane to continue filtering beer.

30. The method of claim 28 or 29, wherein said filtration is halted when the streaming or zeta potential of said porous membrane is reduced to 20% of its original value for the unused porous membrane.

31. The method of any of claims 1-30, wherein said porous membrane is a polyamide porous membrane.

32. The method of claim 31, wherein said filtration is halted when the zeta potential of said porous membrane exceeds -5 mV as measured at pH 4.2.

33. The method of any of claims 1-32, wherein said filtering beer is cold-filtering beer.

34. A filtration unit for filtering beer comprising a feeder line for the filtration-bound beer, a porous membrane, a run-off line for the filtered beer, and means for monitoring the streaming potential and/or zeta potential of said porous membrane through which beer flows. The filtration unit of claim 34, further comprising a bypass porous membrane through which beer :flows, wherein said monitoring means for monitoring the 20 streaming potential and/or zeta potential does so with respect to said bypass porous membrane.

36. A method, according to claim 1, 4 or 29, substantially as hereinbefore described with reference to any one of the examples or drawings.

37. A filtration unit, according to claim 34, substantially as hereinbefore described with reference to any one of the examples or drawings. S eDATED: 26 October, 1999 S PHILLIPS ORMONDE FITZPATRICK Attorneys for: PALL CORPORATION