AU720485B2 - Reporter cell line system for detecting cytomegalovirus and identifying modulators of viral gene expression - Google Patents

Reporter cell line system for detecting cytomegalovirus and identifying modulators of viral gene expression Download PDF

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Publication number
AU720485B2
AU720485B2 AU24439/97A AU2443997A AU720485B2 AU 720485 B2 AU720485 B2 AU 720485B2 AU 24439/97 A AU24439/97 A AU 24439/97A AU 2443997 A AU2443997 A AU 2443997A AU 720485 B2 AU720485 B2 AU 720485B2
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AU
Australia
Prior art keywords
reporter gene
promoter
sample
cytomegalovirus
cell
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
AU24439/97A
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English (en)
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AU2443997A (en
Inventor
Miguel S. Barbosa
Carla M. Suto
Jun Wu
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Signal Pharmaceuticals LLC
Original Assignee
Signal Pharmaceuticals LLC
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Signal Pharmaceuticals LLC filed Critical Signal Pharmaceuticals LLC
Publication of AU2443997A publication Critical patent/AU2443997A/en
Application granted granted Critical
Publication of AU720485B2 publication Critical patent/AU720485B2/en
Anticipated expiration legal-status Critical
Ceased legal-status Critical Current

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6897—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids involving reporter genes operably linked to promoters
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10S435/00—Chemistry: molecular biology and microbiology
    • Y10S435/81—Packaged device or kit

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  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • Biophysics (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Biochemistry (AREA)
  • Physics & Mathematics (AREA)
  • General Health & Medical Sciences (AREA)
  • Biomedical Technology (AREA)
  • Immunology (AREA)
  • Analytical Chemistry (AREA)
  • Plant Pathology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
AU24439/97A 1996-04-10 1997-04-08 Reporter cell line system for detecting cytomegalovirus and identifying modulators of viral gene expression Ceased AU720485B2 (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US08/630,182 US5945276A (en) 1996-04-10 1996-04-10 Reporter cell line system for detecting cytomegalovirus and identifying modulators of viral gene expression
US08/630182 1996-04-10
PCT/US1997/005713 WO1997038139A1 (en) 1996-04-10 1997-04-08 Reporter cell line system for detecting cytomegalovirus and identifying modulators of viral gene expression

Publications (2)

Publication Number Publication Date
AU2443997A AU2443997A (en) 1997-10-29
AU720485B2 true AU720485B2 (en) 2000-06-01

Family

ID=24526131

Family Applications (1)

Application Number Title Priority Date Filing Date
AU24439/97A Ceased AU720485B2 (en) 1996-04-10 1997-04-08 Reporter cell line system for detecting cytomegalovirus and identifying modulators of viral gene expression

Country Status (9)

Country Link
US (1) US5945276A (es)
EP (1) EP0954613B1 (es)
JP (1) JP2000508173A (es)
AT (1) ATE224459T1 (es)
AU (1) AU720485B2 (es)
CA (1) CA2250967A1 (es)
DE (1) DE69715672T2 (es)
ES (1) ES2186889T3 (es)
WO (1) WO1997038139A1 (es)

Families Citing this family (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
PL186473B1 (pl) 1996-01-26 2004-01-30 Virco Nv Sposób oceny in vitro chemioterapii HIV u pacjentów HIV dodatnich oraz sposób oznaczania fenotypowej wrażliwości lekowej poszczególnych szczepów HIV u pacjenta na inhibitory co najmniej dwóch enzymówkodowanych przez gen pol HIV
US5955275A (en) 1997-02-14 1999-09-21 Arcaris, Inc. Methods for identifying nucleic acid sequences encoding agents that affect cellular phenotypes
US6623922B1 (en) 1997-02-14 2003-09-23 Deltagen Proteomics Methods for identifying, characterizing, and evolving cell-type specific CIS regulatory elements
WO2001007061A1 (en) * 1999-07-27 2001-02-01 Smithkline Beecham Corporation Whole cell assay
DE10119428A1 (de) * 2001-04-20 2002-10-24 Enron Wind Gmbh Grundrahmen zur Anordnung der Welle des Rotors einer Windkraftanlage an deren Turm
JP5712513B2 (ja) * 2010-07-07 2015-05-07 富士レビオ株式会社 ヒトサイトメガロウイルス感染の検出方法
US10106817B2 (en) * 2013-02-14 2018-10-23 The J. David Gladstone Institutes Compositions and methods of use thereof for identifying anti-viral agents
CA3012409A1 (en) 2016-01-29 2017-08-03 Sirion Biotech Gmbh Aav-based conditional expression system

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0521427A1 (en) * 1991-07-05 1993-01-07 American Cyanamid Company Method for identifying non-essential genes of the human cytomegalovirus genome and for screening for inhibitors of human cytomegalovirus

Family Cites Families (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5168062A (en) * 1985-01-30 1992-12-01 University Of Iowa Research Foundation Transfer vectors and microorganisms containing human cytomegalovirus immediate-early promoter-regulatory DNA sequence
US5070012A (en) * 1988-03-30 1991-12-03 The Board Of Trustees Of The Leland Stanford Junior University Monitoring of cells and trans-activating transcription elements
US5776502A (en) * 1989-07-18 1998-07-07 Oncogene Science, Inc. Methods of transcriptionally modulating gene expression
WO1992012635A1 (en) * 1991-01-18 1992-08-06 Oncogene Science, Inc. Methods of transcriptionally modulating gene expression of viral genes and other genes
GB9114437D0 (en) * 1991-07-03 1991-08-21 Health Lab Service Board Recombinant adenoviruses and the use thereof for detecting the presence in eukaryotic cells of the expression products of specific genes
US5418132A (en) * 1992-06-18 1995-05-23 Washington University Genetically engineered cell line for detecting infectious herpes simplex virus and method therefore
US5591579A (en) * 1993-12-21 1997-01-07 Washington University Indicator cell line for detecting RNA viruses and method therefor

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0521427A1 (en) * 1991-07-05 1993-01-07 American Cyanamid Company Method for identifying non-essential genes of the human cytomegalovirus genome and for screening for inhibitors of human cytomegalovirus

Also Published As

Publication number Publication date
WO1997038139A1 (en) 1997-10-16
US5945276A (en) 1999-08-31
AU2443997A (en) 1997-10-29
DE69715672T2 (de) 2003-05-22
JP2000508173A (ja) 2000-07-04
EP0954613A1 (en) 1999-11-10
EP0954613B1 (en) 2002-09-18
ATE224459T1 (de) 2002-10-15
CA2250967A1 (en) 1997-10-16
DE69715672D1 (de) 2002-10-24
ES2186889T3 (es) 2003-05-16

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FGA Letters patent sealed or granted (standard patent)
MK14 Patent ceased section 143(a) (annual fees not paid) or expired