AU678785B2 - Purified form of streptogramins, its preparation and pharmaceutical compositions containing it - Google Patents

Description

I

AUSTRALIA

PATENTS ACT 1990 COMPLETE SPECIFICATION NAME OF APPLICANT(S): Rhone-Poulenc Rorer S.A.

ADDRESS FOR SERVICE: DAVIES COLLISON CAVE Patent Attorneys 1 Little Collins Street, Melbourne, 3000.

INVENTION TITLE: o

D

e e o r ro e Purified form of streptogramins, its preparation and pharmaceutical containing it The following statement is a full description of this invention, including the of performing it known to me/us:compositions best method 0 0 I II~ II I IU L- The present invention relates to a purified form of streptogramins comprising a group B component of streptogramins combined with a group A component.

Among known streptogramins, pristinamycin (RP 7293), an antibacterial of natural origin produced by Streptomyces pristinaespiralis, was isolated for the first time in 1955. Pristinamycin marketed under the name Pyostacine® consists mainly of pristinamycin IA and pristinamycin IIA.

Another antibacterial of the streptogramin class, namely virginiamycin, has been prepared from Streptomyces virginiae, ATCC 13161 [Antibiotics and 15 Chemotherapy, 5, 632 (1955]. Virginiamycin (Staphylomycine®) consists mainly of factor S and factor M 1 In United States Patent US 3,325,359, pharmaceutical compositions comprising antibiotic substances constituting antibiotic 899, namely factor S and factor M 1 have been described.

In French Patent Application FR 2,619,008, the use of group A and group B components for the treatment of acne has been described.

25 The antibacterials of natural origin of the O V streptogramin class consist of a mixture of 2 groups of components: group B components and group A components, each group having an antibacterial activity of its own.

1IILI----- C4 I~ ~q~ssP C~ 2 It has been demonstrated that the combination made up of the 2 groups of components produces a synergy of action which results in an enhanced bacteriostatic and bactericidal activity and in a broadening of the spectrum of activity.

In Streptogramine als Modelsysteme fur den Kationentransport durch Membranen, Dissertation zur Erlangung des Doktorgrades der Mathematisch- Naturwissenschaftlichen Facultat der Georg-August UniversitAt zu G6ttingen, G6ttingen 1979, in Antibiotics III, 521 (1975) and in Antibiotic- of the virginiamycin family, Inhibitors which contain synergistic components, C. Cocito, Microbiological Reviews, 145-98 (1979), groups A and B components of streptogramins have been described. J. Preud'Homme, P. Tarridec and A. Belloc, Bull. Soc. Chim. Fr., 2, 585 (1968) have also described natural pristinamycin as well as the different components of which it is made up.

All attempts to make purified combinations of streptogramins invariably involve the group A majority component [namely pristinamycin IIA (PIIA)] which is considered to be responsible for the activity and for the synergy of action. The conclusion from some studies 25 has pointed, moreover, to the importance of this component, which produces better synergy: see European Specification EP 506,561 (page However, these attempts have never been ~0 Ius I La 1 3 crowned with success, partly because of the difficulties of industrial preparation, but mainly because purified pristinamycin IIA is a crystallide product whose bioavailability has proved to be too low for it to be the active principle of a medicinal product.

From the standpoint of the industrial preparation of such products, the techniques available had not hitherto made it possible to obtain, on a preparative scale, a sufficiently purified form, and the production of batches of sufficiently constant and reproducible quality, to satisfy the legal requirements of some countries concerning registration.

For example, industrial batches of natural pristinamycin contain, after purification, impurities in an amount which can reach 20 The attempts at purification carried out hitherto have invariably ended in failure and very often in degradation of one of the groups of components, because these are labile products for which many operations lead to opening of the ring structure or to dehydration of the group A components.

SAs a result, for many years it was considered that an improvement in the degree of purity could not be achieved. In 1988, purification was still considered to be a problem: see J. of Liq. Chromatography, 11 (11), 2367 (1988). Also in 1988, N.K. SHARMA and M.J.O.

ANTEUNIS likewise declared that separation and purification of the components of virginiamycin were aa~ r a possible for analytical purposes, but could not be envisaged for the industrial production of the products because of the difficulties encountered: Bull. Soc.

Chim. Belg., 97 193 (1988), Because of this situation, the marketing of pristinamycin (Pyostacine®) has been limited to certain countries such as France and Belgium. The same applied to virginiamycin (Staphylomycine®), marketed only in a limited number of countries for human medicine, as well as to mikamycin whose marketing (limited to Japan) has now been stopped. Thus some populations have been deprived of a treatment potentially useful in severe infections caused by Gram-positive cocci (in particular infections caused by methicillin-resistant staphylococci), or in sexually transmitted diseases.

In the field of antibacterial, it is well known by practitioners that allergies or resistances may develop after administration of some classes of antibiotics [The New England Journal of Medicine, 324 601 (1991)]. In the hospital environment, especially, many resistant strains of Staphylococcus aureus are known. For this reason, it is extremely useful for the doctor to have at his disposal a wide range of chemically different classes of antibacterial 25 agent so as to be able to match the treatment to the o particular case of the patient to be treated. The consequence of the failure of a particular class of marketed antibacterial can be very serious, or even dramatic, because it can result in patients who do not tolerate other classes of antibiotic being deprived of treatment.

Thus, attempts made at purification were always directed towards removing the minority components of streptogramins, these being regarded as non-essential and, for the most part, as impurities.

Among the group A components of natural streptogramins, pristinamycin IIB (PIIB) is a minority component whose proportion by weight is less than 10 in natural pristinamycin, and most often of the order of 8 or even of the order of 6 in virginiamycin.

It has now been found, and this forms the subject of the present invention, that the combination 15 consisting of one or more group B components of general formula:

°YY-

CH3 0 Al 0 o' N/ Ir in which A, is a radical of general formula:

Y

1R' 0

N

O

0 or N(CH3) 2 0 NH

OH

0 (la') 0 .e Wherein R' is a hydrogen atom or a hydroxyl radical and Y is a hydrogen atom, a methylamino radical or a dimethylamino radical, R is an ethyl radical or, when R' represents a hydrogen atom, R can also represent a methyl radical, and R, and R 2 each represent a hydrogen atom, or alternatively A, is a radical of formula: O ICH 3 0 0 N H3 (Ib) CH3 0 CH3 R is an isobutyl radical, and 10 R. is a hydroxyl radical and R 2 is a methyl radical, and one or more group A "minority" components of general formula: o* e eee I 'ILI I Y dd

I

7 0

OH

°3C CH3 0 0 (II) 0 N R" 0 in which R" is a hydrogen atom or a methyl or ethyl radical, in the form of a cocrystallizate, a coprecipitate or a finely divided physical mixture is especially advantageous on account of its biological (i.e antibacterial) activity in vivo.

The combinations according to the invention manifest a biological action in vivo which is markedly greater than that of a corresponding natural product (for example natural virginiamycin or natural 10 pristinamycin) and than that of combinations involving the group A majority component. Furthermore, the new combinations are endowed with satisfactory bioavailability, and may be prepared on a large scale.

It is thus possible to gain access to a 15 purified and bioavailable form of a final product having a good level of activity and naturally containing less than 6 of impurities.

The product of general formula (II) in which R" is an ethyl radical, hereinafter referred to as pristinamycin IIF (PIIF), and the product of general I La ~J~p I formula (II) in which R" is a hydrogen atom, hereinafter referred to as pristinamycin IIG (PIIG), are new products which are streptogramin components of very low abundance, the proportion of which by weight is less than 0.5 in batches of natural product.

The combinations according to the invention are advantageously prepared with the components in proportions of 10:90 to 90:10 (by weight), or preferably in proportions of 20:80 to 80:20. They maybe in the form of a physical mixture of powders; or, according to an aspect of the present invention, in the form of a coprecipitate; or, according to a further aspect of the invention, in the form of a cocrystallizate, described in more detail below.

The present invention also provides purified forms consisting of the cocrystallized combination of at least one group B component of general formula (I) with at least one group A component of general formula o (II The cocrystallisation takes place in the constant stoichiometry of 1 mol of component(s) of general formula to 2 mol of group A component(s) of general formula (II) [this stoichiometry corresponding to the relative proportion of approximately 43-44:57-56 25 by weight in the case where the component A is a product of general formula for which A, is of structure

L

IIPIP~

S

5* The cocrystallized combination of the invention may be used as a purified and stable antimicrobial agent also possessing improved in vivo activity as well as good bioavailability, or as a means of purification of a minority component of streptogramins of the general formula (II).

Heretofore it has never been possible to purify a group A component of general formula (II) by crystallization. As a result, hitherto, only chromatographic methods were known for preparing a purified group A product of general formula and no other means of purification was known for isolating these products in large amounts.

It has now been found that the group A component of general formula (II) may be obtained in the pure form by proceeding via the cocrystallized combination defined above. A crude mixture containing at least 30 of a group A minority component corresponding to the general formula dissolved in an organic solvent such'as a ketone (acetone, methyl ethyl ketone, methyl isobutyl ketone, for example), an ester (ethyl acetate, isopropyl acetate, butyl acetate, isobutyl acetate, for example), a chlorinated solvent (methylene chloride, chloroform, 1,2-dichloroethane, for example), or a nitrile (acetonitrile for example), and to which a group B component defined by the general formula is added, gives a cocrystalliza compound in the proportions defined above. The amount of 7 -r0VT compound of general formula introduced is appropriately chosen so that the residual concentration of this product (after the cocrystallization) is less than its solubility in the medium. It is also understood that variations in the respective contents of the initial medium with respect to the product of general formula (II) and with respect to the product of general formula do not bring about a modification of the cocrystallizfe compound obtained. The cocrystallized combination thereby obtained, dissolved in a solvent such as, for example, methyl isobutyl ketone or dichloroethane and treat. 1 in an acid medium (sulphuric acid, hydrochloric acid, for example), makes it possible to obtain, after treatment of the organic phase with a solvent such as, for example, hexane, the group A minority component purified and free from group B component.

This cocrystallized combination affords, moreover, the advantages of greatly enhanced stability high purity, and, most p.rticularly, ready industrialization.

This method may also be adapted to the preparation of cocrystallizates with modified derivatives of the natural group B components of streptogramins. These cocrystallizates also fall within the scope of the present invention. Similarly, i the preparation of the purified forms of minority components of general'formula (II) from such sl slll-r~llp~rq cocrystallizates falls within the scope of the present invention.

A preferred embodiment of the invention is a combination of pristinamycin IB [as defined above when AI represents a radical of general formula (Ia) for which Y is a methylamino radical, R' is a hydrogen atom, and R is an ethyl radical] or of virginianycin S.

[as defined above when A. represents a radical of general formula (la) for which Y and R' are hydrogen atoms, and R is an ethyl radical] or of a mixture of virginiamycin Si and virginiamycin S 4 [as defined above when A 1 is defined as for virginiamycin Si and R is a methyl radical] with pristinamycin IIB las defined above by the general formula (II) in which R" is methyl] and containing less than 6 of impurities, and preferably less than 3 of impurities.

e" Another preferred embodiment of the invention S. consists of a combination of a group B component of streptogramins defined by the general formula and a group A component of general formula (II) containing a relative proportion of the groups B and A components in a constant mole ratio of the order of 1:2.

According to a feature of the invention, the new combinations of at least one group B component of streptogramins of general formula and at least one group A component of general formula (II) may be obtained, for example, by preparation of the S, L cocrystalliz7de compound as is defined above. When it is -I II desired to obtain a combination in different proportions, the cocrystallizce compound thus prepared may be combined with at least one of the components of general formula or with at least one group A component of general formula these components being purified beforehand, in an appropriate amount for obtaining the desired proportions, or else the group A component of general formula (II) (or mixture of such components) may be purified from the cocrystallizate and then mixed in the desired proportions with one or more group B components of general formula Alternatively, the combinations according to the invention may be prepared after isolation of the group B component(s) and of the group A component of general formula (II) from the corresponding natural streptogramin, by purification of each of these components, followed by mixing of the purified components in the desired proportions as defined above.

The combinations according to the invention may also be coprecipitated in the desired proportions from a solution of the components of general formulae *9* and (II) [or alternatively from a solution of the cocrystallizate and of one of the components of general formula or in methyl isobutyl ketone or in acetone or methylene chloride, the solution being poured, for example, into hexane or cyclohexane or into a T wate,.

INT o 4} I- I I-d 81 L: ~s The preparation and separation of the groups A and B components are performed by fermentation and isolation of the constituents from the fermentation medium according to, or by analogy with, the method described by J. Preud'homme et al., Bull. Soc. Chim.

Fr., vol. 2, 585 (1968), in Antibiot. Chemother., 632 (1955) or 2, 606 (1957), in Chromatog. Sym., Brussels, 181 (1962), in Antibiot. Ann., 728 784 (1954-55), in Patent US 3,299,047 or in Streptogramine als Modelsysteme fur den Kationentransport durch Membranen, Dissertation zur Erlangung des Doktorgrades der Mathematisch-Naturwissenschaftlichen Facultat der Georg-August Universitat zu G6ttingen, G6ttingen 1979, or as described below in the examples. In particular, in the case of pristinamycins, the separation of the groups A and B components may be performed by suspending crude streptogramin in an organic solvent a such as an acetate (ethyl acetate for example), followed by filtration or centrifugation of the crude group A component and by extraction of the group B component in an acidic aqueous medium followed by a reextraction in a methylene chloride medium. The separation of groups A and B components may also be performed by acid extraction of a solution of crude streptogramin in methyl isobutyl ketone, followed by *pe isolation by extraction of the group B component from the aqueous phase and isolation of the group A component by precipitation from the organic phase.

After separation, purification of the group B components of streptogramins may be carried out by crystallization in an alcohol such as ethanol, methanol or isopropanol, in an acetate (isopropyl acetate or butyl acetate, for example), in a ketone (methyl ethyl ketone for example) or in acetonitrile, or by chromatography. Purification of the group A components of general formula (II) may be performed by chromatography, eluting with an acetonitrile/water mixture.

Alternatively, the preparation of the groups A and B components of general formulae (II) and respectively, is performed as described in French Patent Application 2,689,518, by separate fermentation according to the following steps: first step (optional), mutagenesis on a nonselective microorganism producing streptogramins, and second step, selection of the selective microorganisms.

I* The non-selective microorganisms are generally actinomycetes and fungi. The starting microorganisms which can be used in the process are, in particular, microorganisms which are non-selective producers of a streptogramin chosen from the group comprising pristinamycin, virginiamycin, mikamycin, ostreogrycin, viridogrisein, vernamycin and etamycin.

As an example, some non-selective microorganisms which

I

9 9 may be employed are named below in the table.

MICROORGANISMS ANTIBIOTICS Micromonospora sp. vernamycin

STREPTOMYCES

S. alborectus virginiamycin S. cariseus (NRRL2426) viridogrisein S. lavendulae etamycin S. loidensis (ATCC11415) vernamycin S. mitakaensis (ATCC15297) tnikarnycin S. ostreogriseus (ATCC27455) ostreogrycin S. pristinaespiralis (ATCC25486) pristinamycin S. virginiae (ATCC13161) virginiamycin ACT INOMYCES A. dacrhestanicus etamycin More especially, the preparation is carried out with microorganisms chosen from Strentomvces alborectus,, Streptomyce.s mitakaensis, Streptomyces Pristinaespiralis, Streptomyces ostreocrriseus and Streptomyces virginiae.

The first step of the preparation consists in miodifying the non-selective microorganism so as to increase its overall capacity for antibiotic production and/or so that it synthesizes only one of the two components of streptogramins. This may be obtained by genetic modifications (mutation in structural genes for 16 enzymes involved in the pathway of biosynthesis, or in sequences permitting the expression of such structural genes, for example) or biochemical modifications (modification of a post-translational mechanism, impairment of a feedback-inhibition mechanism, and the like). Various mutagenesis tools are used: physical agents: X-rays, ultraviolet rays; or chemical agents: alkylating agents such as ethyl methanesulphonate (EMS), N-methyl-N'-nitro-Nnitrosoguanidine (Delic et al. Mutation Res. 9 (1970) 167-182) or 4-nitroquinoline 1-oxide (NQO); bialkylating agents; intercalating agents; or any system of mutational insertion into DNA, and especially transposons, integrative plasmids, phages or prophages; or alternatively protoplast fusion (Cohen, Nature 268 (1977) 171-174).

These tools (alone or in combination) may be applied to the non-selective microorganisms in the form of spores or of germinated or germinating spores, or to mycelium. The preparation may also make use of manipulations (at random or directly) that enable microorganisms capable of selectively producing one component of streptogramins from non-selective microorganisms to be obtained.

The second step of the preparation relates to the identification and isolatipn of the selective microorganisms. This step may be carried out, in IB I-dLu I L~~C.L I particular, by means of a test of sensitivity with respect to a microbe. Various microbes which are specifically sensitive to group A components or to those of group B of streptogramins exist: for example, Bacillus subtilis (ATCC6633), Bacillus circulans, Bacillus cereus (Watanabe, J. Antibio. Ser. A XIII 1) (1960) 62) or C. xerosis (Watanabe, loc. cit.), which are specifically sensitive to group B components; Streptococcus acalactiae B96 (Antimicrob. Agents Chemother. 10(5) (1976) 795), Micrococcus luteus (Prikrylova, loc. cit.) or Sarcina lutea (ATCC9341), which are specifically sensitive to group A components.

It is also possible to prepare artificially microbes which are specifically sensitive to one component of streptogramins by inserting a gene for resistance to one of the 2 components of streptogramins into a microbe which is sensitive to both of them. Some of these genes have been cloned (Le Goffic et al., J. Antibio. XXX(8), 665 (1977); Le Goffic ut al., Ann.

Microbiol. Inst. Pasteur 128B, 471 (1977); Solh et al., Path. Biol. 32(5), 362, (1984)); such genes are introduced into different microbes by standard techniques of molecular biology. The selection step may also be performed by an ELISA test using specific antibodies for the components A or B, or alternatively by analytical techniques such as chromatography (liquid chromatography, thin-layer chromatography, and the like). In the case of a test of sensitivity with L ISL C I respect to a microbe, it is, in addition, preferable to validate the selection by chromatographic assay.

Thus, according to the invention, it is now possible to obtain on an industrial scale a new purified form of streptogramin in which the level of impurities, the definition and the constancy of the composition complies with the legal requirements of the registration, and which furthermore possesses improved in vivo activity and bioavailability as well as lower toxicity. The new combination may thus remedy the lack in many countries of an acceptable treatment with an antibacterial of this class.

The new combination of a group B component of streptogramins of general formula and a group A component of streptogramins of general formula (II) displays especially advantageous in vivo activity "against Gram-positive microbes in particular. In vivo, in mice, it was shown to be active against Staphylococcus aureus IP 8203 at doses of 30 to 50 mg/kg administered orally.

As an example, the oral CD 5 S of several combinations of the components of general formulae (I) and I21) in the experimental Staphylococcus aureus IP 8203 infection of mice is given below.

In Table I below, the combinations studied are prepared by coprecipitation in hexane from a solution of the components of general formulae aid (II) in methyl isobutyl ketone or in acetone: p~T 0

B

D

II

e Product (I)/product (II) C, (mg/kg) p.o.

combination: PI (Example 1)/PIIB (Example 18) 10:90 44 20:80 32 30:70 70:30 80:20 90:10 Table I In Table II below, the combinations illustrated are cocrystallized products prepared as described in the examples.

Cocrystallized product CDs, (mg/kg) p.o.

product (II) combination PI/PIIB (Example 9) 38 PIA/PIIB (Example 11) 28 PIB/PIIB (Example 12) 32 PIC/PIIB (Example 13) 36 PID/PIIB (Example 14) Factor S/PIIB (Example 15) 32 Factor S,/PIIB (Example 16) Factor S/PIIF (Example 17) ee soe oo ee W Table II L 4 In Table III below, the combination described is prepared in the form of a physical mixture of the powders.

Product (I)/product (II) combination CDs (mg/kg) p.o.

PIA (Example 1)/PIIB (Example 18) 30:70 36 PIA (Example 1)/PIIB (Example 18) 50:50 Factor S (Example 5)/PIIB (Example 18) 30:70 44 o c o o o e o o

D

Tzble III Furthermore, the new combination does not display toxicity: no sign of toxicity manifests itself in mice at a dose of 150 mg/kg administered orally (2 administrations).

According to another feature of the invention, when the cocrystallized combination is used as a means of purification of the component of general formula the latter may be obtained by acid extraction of a solution of the cocrystalliz* compound in a ketone (methyl isobutyl ketone for example), followed by isolation by extraction of the group A component by precipitation from the organic phase.

The examples which follow illustrate the present invention. In the examples, the assays are given in by weight.

Ir -Q~ SEPARATION AND PURIFICATION OF GROUP B COMPONENTS: Example 1 kg of crude pristinamycin [pristinamycin IA (PIA): 20.7 pristinamycin IB (PIB): 3.9 pristinamycin IC (PIC): 0.6 pristinamycin ID (PID): 0.3 pristinamycin IIB (PIIB): 8 pristinamycin IIA (PIIA): 45 pristinamycin IIF (PTIF): 0.5 (not assayed), pristinamycin IIG (PIIG): 0.5 (not assayed)] are suspended in 210 litres of ethyl acetate and stirred for 15 hours at room temperature. The suspension is filtered and the ethyl acetate filtrate is collected and extracted with twice 20 litres of IN sulphuric acid and then 20 litres of distilled water.

The combined aqueous phases are washed with 6 times 15 litres of ethyl acetate, then adjusted to pH 7 by adding 30 litres of 10 sodium bicarbonate solution and extracted with 3 times 30 litres of methylene chloride. The methylene chloride phases are combined Sand washed with 10 litres of distilled water. The methylene chloride is then distilled off and replaced by 50 litres of ethanol. The mixture is then treated *555** S" under reflux with 0.8 kg of L3S charcoal for minutes. After filtration and washing with twice litres of ethanol, the mixture is cooled to 10 0 C in the course of 15 hours. After being maintained for one hour at 100C, the suspension is filtered and washed with 3 times 7 litres of ethanol. After drying of the solid at 40 0 C under redi..red pressure, 5.7 kg of I- -r -r -IC I I purified pristinamycin I (hereinafter referred to as PI) are obtained.

Assay: 96.8 (PIA: 81.1 PIB: 12 PIC: 2.6 PID: 1.1 Yield with respect to PIA: 74 1500 g of purified PI are taken up with 9 litres of 1,2-dichloroethane, and 1.5 equivalents of succinic anhydride and 0.015 equivalent of dimethylaminopyridine are then added. The solution is maintained for 1 week at 20 0 C and then introduced onto a column containing 10 kg of silica (20-45 pm) [column c height: 1 m; diameter: 20 cm). Elution is performed by

*SO*

percolation of a 1,2-dichloroethane/methanol mixture at a flow rate of 18 litres/hour for 6 hours; the percentage of methanol (water content 5 is increased from 0 to 4 during the chromatographic run, 47 2.4-litre fractions are recovered.

-e Fractions 5 to 15 are pooled, and tL,.

1,2-dichloroethane is evaporated off and replaced by 0** 5 litres of ethanol. After cryst'llization, 365 g of PIA assaying at 99.8 are obtained.

Example. 2 Fractions 36 to 39 from the chromatographic run described in Example 1 are pooled and the 1,2-dichloroethane is distilled off; 210 g of solid are thereby obtained. 40 g of this solid are taken up with 8 litres of water to which 8 cm 3 of O1N hydrochloric acid are added. After 3 hours at 90 0 C, the solution is neutralized to pH 6.5 with sodium hydrogen carbonate.

The solution is extracted with 3 times 1 litre of ethyl acetate and the extract is washed with twice 0.2 litre of water. After treatment with charcoal, thu ethyl acetate is evaporated off and replaced by 600 cm 3 of ethanol. After recrystallization, 20 g of PIB assaying at 97 are obtained.

Examplf 3 FrLctions 22 to 26 from the chromatographic run described in Example 1 are pooled and the 1,2-d-chloroethane is distilled off; 139 g of solid are thereby obtained. This solid is taken up with a minimum of 1,2-dichloroethane and introduced onto a silica column. Elution is performed by percolation of a 1,2-dichloroethane/methanol mixture at a flow rate of 18 litres/hour for 6 hours; the percentage of methanol (water content 5 is increased from 0 to 5 during the chromatographic run. 48 2.4-litre fractions are recovered. Fractions 38 to 43 are evaporated and the solid is taken up with 300 cm 3 of ethanol. After recrystallization, 22 g of PI containing 40 of PIC are obtained. Successive chromatographic runs on silica (20-45 Am) with percolation using a methylene chloride/methanol (98:2 by volume) eluent enable 5 g of a solid to be obtained, which solid, after vigorous agitation with methyl isobutyl ketone and recrystall!zation in ethanol, assays at 95 with respect to PIC.

I 1 31 IIIC li~l Example 4 1000 g of PI, obtained as described above in Example 1, are dissolved in the minimum of chloroform and purified in successive fractions on a column of silica (20-45 Am). After elution with chloroform containing 2 to 5 of methanol, a product is obtained and is concentrated to dryness. This product is then purified by 2 successive runs on a column of Diaion® resin percolated with an acetonitrile/water (60:40 by volume) mixture. The fractions are monitored by chromatography. The fractions containing PID are pooled and concentrated to dryness. Approximately 3 g of product assaying at 60 with respect to PID are thereby obtained. A further purification is performed by countercurrent chromatography using a methyl isobutyl ketone/acetone/formic acid (40:2:40 by volume) solvent mixture. Concentration of the fractions containing PID to dryness enables 1 g of solid assaying ,at 95 with respect to PID to be obtained.

Example 400 g of Staphylomycine® (in tablet form @*to: initial composition: virginiamycin S, 3.4 virginiamycin S 4 0.9 are introduced into 4 litres of water.

The tablets are disintegrated by stirring for minutes at 20 0 C. 1 litre of methylene chloride is added and stirring is continued for 1 hour. Next, after settling has taken place, the methylene chloride phase a ~I is separated and filtered and is then run in the course of 30 minutes into a volume of 5 litres of stirred hexane. After 1 hour of stirring, the suspension ir filtered and a solid is collected and washed with 3 times 250 cm 3 of hexane. After drying, 52 g of solid are recovered and suspended in 370 cm 3 of ethyl acetate. The suspension is agitated vigorously twice in successicn at 20 0 C and for a period of 18 hours. The filtrate corresponding to each agitation treatment is taken to dryness and then dissolved in 850 cm 3 of methanol under reflux. After a gradual fall in the temperature to -20 0 C in the course of 16 hours, a solid is collected by filtration and washed with a small amount of methanol. After drying of the solid at 35 0 C under 15 reduced pressure, 9 g of factor S (virginiamycin S) are obtained.

Assay: 96 (SI: 75.4 S4: 20.6 Yield with respect to factor Si (virginiamycin 50 Example 6 20 1 g of factor S, obtained as described above in Example 5 and dissolved in acetonitrile in the proportion of 125 mg/cm 3 is purified in 4 operations by chromatography on a Nucleosil 5C8® column (height cm, external diameter 2.54 cm), injecting a volume of 2 cm 3 and eluting with a water/acetonitrile (60:40 by volume) mixture at a flow rate of 7.5 cm 3 /minute. On each occasion, a volume of 120 cm 3 containing factor S.

is collected, equivalent to 480 cm 3 in all. The I I ~a 91P IPI Il--a chromatographic procedure is repeated 4 times in order to treat the whole of the 1 g of factor S. A volume of approximately 500 cm 3 containing factor S, is thereby collected. The acetonitrile is removed using a rotary evaporator. The aqueous phase is extracted with 3 times cm 3 of dichloromethane. The methylene chloride phases are combined, washed with 50 cm 3 of distilled water, dried over sodium sulphate and filtered. The dichloromethane is eliminated using a rotary evaporator under reduced pressure (5 mm of mercury). 0.67 g of factor S, assaying at 99.6 is thereby obtained.

ot PREPARATION OF CRUDE GROUP A COMPONENTS: Example 7 S: 500 g of crude pristinamycin [pristinamycin 99 IA (PIA): 20.7 pristinamycin IB (PIB): 3.9 pristinamycin IC (PIC): 0.6 pristina.iycin ID (PID): 0.3 pristinamycin IIB (PIIB): 8 pristinamycin IIA (PIIA): 45 are dissolved in 50 litres of methyl isobutyl. ketone. This solution is extracted 5 times with an aqueous phase composed of 2.5 litres of water and 2.5 2itres of IN sulphuric acid, and then washed with 3 times 10 litres of water. The methyl isobutyl ketone is then treated with 7.5 litres of an aqueous sodium hydrogen carbonate solution containing 35 g/litre, and then washed with 5 litres of water. On each occasion, the aqueous phase is mixed with the organic phase, settling is allowed to take place and the aqueous phase is separated.

r~ll The organic phase obtained is brought into contact with 750 g of alumina, filtered, concentrated to a volume of approximately 4 litres and taken up with volumes of hexane. The precipitate obtained is filtered off and dried. 300 g of product are obtained, which product is suspended in 1 litre of isopror-nol.

After stirring at 55 0 C for 45 minutes, the suspension is filtered at 4 0 C. The filtration mother liquors are concentrated to dryness, and the residue is taken up 10 with 500 cm 3 of methyl isobutyl ketone into which a e volumes of hexane are poured. The precipitate is a filtered off, washed with hexane and dried at 40 0

C

under reduced pressure. 69 g of crude PIIB, containing 36 of PIIB and 6 of PIIA and no longer containing PIA, are obtained.

Example 8 g of crude PIIB, obtained as above in Example 7, are purified in several operations by chromatography on a Nucleosil 5C8® column (column diameter 5 cm, height 30 cm) percolated with a 60:40 •water/acetonitrile eluent. 250 mg of pristinamycin IIF (PIIF) are thereby obtained.

PREPARATION OF A COCRYSTALLIZED PRODUCT: In the examples which follow, it has been demonstrated that the X-ray diffraction spectrum of the cocrystallized product is different from the spectrum of the group B component crystallized alone in the same solvent, where this component exists.

I

I

28 Example 9 Crude PIIB, obtained above in Example 7, is dissolved in 190 cm 3 of acetone. 33 g of purified PI (PIA: 81.1 PIB: 12 PIC: 2.6 PID: 1.1 are added. After 17 hours of stirring at 20 0 C, a suspension is obtained which is filtered at 4 0 C. The product is washed and dried. After recrystallization at a concentration of 100 g/l in acetone, 10 g of white crystals are obtained assaying at 55 with respect to PIIB+PIIF+PIIG and assaying at 43 with respect to

.PIA+PIB+PIC+PID.

Example 250 mg of purified PIIB, obtained as described below in Example 18, are dissolved in 17 cm 3 of ethyl acetate. 300 mg of purified PI (PIA: 81.1 PIB: 12 PIC: 2.6 PID: 1.1 are added. After hours of stirring at 20 0 C, filtration, washing and drying, 125 mg of white crystals are obtained.

Assay with respect to PIIB+PIIF+PIIG: 56 of which 20 PIIB 54 Assay with respect to PIA+PIB+PIC+PID: 43 Example 11 560 mg of pure PIIB, obtained as described below in Example 18, are dissolved in 5 cm 3 of acetone.

480 mg of PIA (assay 99.8 are added. After 20 hours of stirring at 20 0 C, the suspension is filtered. After washing with 1 cm 3 of acetone and drying for 30 hours at 0 C under reduced pressure 1 kPa), 590 mg of white I crystals are obtained.

Assay with respect to PIIB+PIIF+PIIG: 56 of which PIIB 54 Assay with respect to PIA: 43 The mother liquors from the above crystallization are taken up and a further 560 mg load of PIIB is added. The PIIB/PIA mass ratio is then in the region of 4. After 20 hours of stirring, filtration, washing and drying, 195 mg of crystals, of identical purity and composition to those of the crystals originating from the 1st crop, are obtained.

Example 12 Using the procedure described above in Example 11, but replacing PIA by 480 mg of PIB (assay 97 820 mg of white crystals are obtained assaying at 56 with respect to PIIB+PIIF+PIIG (of which PIIB 54 and assaying at 43 with respect to PIB.

Example 13 Using the procedure described above in Example 11, but employing 680 mg of PIIB and 580 mg of PIC (assay 95 in 4 cm 3 of acetone, 315 mg of white crystals are obtained assaying at 57 with respect to PIIB+PIIF+PIIG (of which PIIB 55 and assaying at 42 with respect to PI (of which 37 PIC).

Example 14 Using the procedure described above in Example 11, but replacing PIA by 480 mg of PID (assay 475 mg of white crystals are obtained assaying L_ sres LI_ I .h 31 cobalt anticathode. The 15.8 A line is assigned the reference value 100. The relative values are estimated by measuring the height of the line after deduction of the continuous background.

oe e e oe ***ee o oea inter- Product of Example planar spacing Ex.9 Ex.11 Ex.12 Ex.13 Ex.14 Ex.15 Ex.16 Ex.17 15.8 100 100 100 100 100 100 100 100 11.8 40 34 35 32 43 28 36 21 10.3 69 52 63 65 50 70 80 87 9.7 38 45 47 44 43 40 49 6.4 44 41 51 41 37 40 51 39 6.1 38 41 40 38 43 30 40 5.9 75 64 70 -63 67 74 89 71 5.8 38 39 4.7 49 50 44 54 5.2 92 75 79 71 70 70 91 81 67 59 60 60 57 54 69 52 4.7 50 43 53 49 47 50 40 42 Table IV The X-ray diffraction diagrams are similar irrespective of the cocrystallized product (the interplanar spacings of the main lines are not significantly different).

PURIFICATION OF A GROUP A COMPONENT: Example 18 9.3 g of the product obtained in Example 9 are dissolved in 490 cm 3 of methyl isobutyl ketone. This solution is extracted twice with 370 cm 3 of 0.5N aqueous sulphuric acid solution and then washed with twice 150 cm 3 of water. The organic phase is then concentrated to a volume of approximately 80 cm 3 and poured into volumes of hexane. The precipitate obtained is washed, filtered off and dried. To remove the methyl isobutyl ketone, the precipitate is then taken up at a concentration of 100 g/l in acetone, poured into volumes of hexane, washed and dried. 3.5 g of a product containing purified PIIB and no longer containing PI are obtained.

20 Assay PIIB+PIIF+PIIG: approximately 95 of which 92

PIIB.

The present invention also provides pharmaceutical compositions which can be used in human or veterinary medicine and which contain as active product the new purified streptogramin combination, comprising at least one group B component of streptogramins combined with the group A component of general formula in the pure form or in the L IIICI ~p 111 33 presence of one or more compatible and pharmaceutically acceptable diluents or adjuvants. These compositions may be used orally or topically. The can contain the combinations according to the invention in the form of a physical mixture of the powders, of a coprecipitate or of a cocrystallizate.

As compositions for oral administration, tablets, hard gelatin capsules, pills, powders, lyophilizates or granules may be used. In these compositions, the active product according to the invention may be mixed with one or more inert diluents or adjuvants such as sucrose, lactose or starch. These compositions can also comprise substances other than diluents, for example a lubricant such as magnesium stearate.

The compositions for topical administration can be, for example, creams, ointments or lotions.

In human or veterinary therapy, the compositions according to the invention are especially 20 useful in the treatment of infections of bacterial origin, in particular severe infections caused by Grampositive cocci: staphylococcal infections (in particular infections caused by methicillin-resistant staphylococci), streptococcal infections (in particular against penicillin- and macrolide-resistant pneumococci); they are also especially useful in the treatment of infections caused by Haemophilus, Moraxella catarrhalis, Neisseria gonorrhoeae, Chlamvdia 1 L- L I ~L II IbBE trachomatis, Mycoplasma hominis, Mycoplasma pneumoniae and Ureaplasma urealyticum.

The compositions according to the invention may be employed, in particular, in the treatment of upper and lower respiratory infections (for example treatment of pulmonary infections), in the treatment of skin infections, in the long-term treatment of bone or joint infections, in the treatment or prophylaxis of endocarditis in dental and urinary surgery, in the treatment of sexually transmitted diseases and also in the treatment of the opportunistic bacterial and parasitic infections occurring in AIDS and as prophylaxis of the risk of Staphylococcus in immunosuppressed patients.

Generally speaking, the doctor will determine the dosage he considers most suitable in accordance with the age, weight, degree of infection and other factors distincti.v to the subject who is to be treated.-Generally, the doses are between 0.4 and 3.5 g 20 of active product taken in 2 or 3 doses daily via the oral route for an adult.

The examples which follow, illustrate compositions according to the invention: Example A Opaque hard gelatin capsules containing a 250 mg dose of the cocrystallized PIB/PIIB combination are prepared according to the usual techniques.

Example B Opaque hard gelatin capsules containing a 250 mg dose of the cocrystallized factor S/PIIB combination are prepared according to the usual techniques.

Example C Tablets containing a 384 mg dose of active product and having the following composition are.

prepared according to the usual techniques: PIB/PIIB (45 %/55 384 mg Hydroxypropylmethylcellulose............ 25 mg Magnesium 35 mg Colloidal 14 mg o qs 700 mg Example D Tablets containing a 384 mg dose of active 15 product and having the following composition are prepared according to the usual techniques: Factor S/IIB (45 %/55 384 mg Hydroxypropylmethylcellulose............ 25 mg S- Magnesium stearate 35 mg 20 Colloidal 14 mg qs 700 mg I c

Claims (15)

1. A purified form of streptogramins, consisting of a combination of one or more group B components of streptogramins, of general formula: N R 2 *0*a e* to oo *g in which A, is a radical of general formula: a a a a e e or/ N(CH 3 2 0 NH OH (la') (la) wherein R' is a hydrogen atom or a hydroxyl radical and Y is a hydrogen atom, a methylamino radical or a dimethylamino radical, R is an ethyl radical or, when R' represents a hydrogen atom, R can also represent a methyl radical, and R, and R 2 each represent a hydrogen atom, or M AI is a radical of formula: 0 0 3 CH3 CH.'NCH, CH 3 0 CH3 R is an isobutyl radical, and R, is a hydroxyl radical and R 2 is a methyl radical, and one or more group A components of streptogramins, of general formula: *H 0 NOH H 3 C CH3 0 0 0 0 *H 3 C 1 0l 0 R" 0 in which R" is a hydrogen atom or a methyl or ethyl radical,-in the form of a cocrystallizate, a coprecipitate or a finely divided physical mixture.

2. A purified form of streptogramins according to claim 1, containing less than 6 of impurities.

3. A purified form of streptogramins according to claim 1 or 2, in which the relative proportions of the said group B components of 15 streptogramins and the said group A mina iy components of streptogramins are from 10:90 to 90:10 by weight. i~ In -Y C I

4. A purified form of streptogramins according to claim 3, in the form of a coprecipitate or a finely divided physical mixt:ure, in which the relative proportions of the said group B components of streptogramins and the said group A minr*,components of streptogramins are from 20:80 to 80:20 by we.Lght. A purified form of streptogramins according to claim 1 or 2, in the form of a c,.cystallizc&e compound of one or more of the said group B compone~nts of otreptogr-amins and one or more of the said group A m&N components of streptogramins in a mole ratio of the order of 1:2. A purified form of straptogramins according to claim Z. substantially as described in any IS one ,f Examples 9 to 17,

7. A process for preparing a purified form of streptcgramins according to any one of claims 1 to 6, which comprisei cocrystallizing the said groupj A component of streptogramins with tie said grou oomponent of streptogramins, and then, if necessary, combining t%.he cocrsallizc~e compound obtained with the appropriate group A or B coomponent(s) to obtain a combination in the desired propor~tio~ns.

8. A process according to claim 7 S Aewro iV M2 Ck 6t M substantially.j rin~r

9. A process for purifying a group A v- ycompont:nt of ritreptogramins as defined in Claim 1, compris'es preparing a cocrystalliId-C~ P:\OI3R\PD\1648859.CLM 0/3/97 39 of this component with one or more group B components of streptogramins, and then removing the group B component(s) by treatment in an acid medium. A process according to claim 9 substantially as described in Example 18.

11. A purified group A component of streptogramins as defined in claim 1, when obtained from a cocrystallizate according to claim

12. A group A component of streptogramins of formula: 0 H N In which R" is a hydrogen atom or an ethyl radical.

13. A cocrystallizate consisting of one or more group B components of streptogramis, and one or more group A components S of streptogramins as defined in claim 1.

14. A group A component of streptogramins according to S. claim 12 substantially as described in Example 8. A cocrystallizate according to Claim 13 substantially 25 as described in any one of Examples 9 to 17.

16. A cocrystallizate according to claim 5 when used in the preparation of a purified form of streptogramins according to any one of claims 1 to 4.

17. A cocrystallizate according to claim 5 when used in the purification of a group A component of streptogramins as 1 Fk; 4N-r o P:\OPER\IDB\1648859.CLM 20/3/97 40 defined in claim 1.

18. One or more group A compounds of streptogramins as defined in claim 1, when used in the preparation of cocrystallizates with one or more group B components of streptogramins.

19. Pharmaceutical composition comprising a purified form of streptogramins according to any one of claims 1 to 5, and a compatible and pharmaceutically acceptable diluent or adjuvant. DATED this NINETEENTH day of MARCH 1997 Rhone-Poulenc Rorer S.A. By DAVIES COLLISON CAVE Patent Attorneys for the applicant 6e **oo oe o m• I•0 I- L/2 ABSTRACT PDRIFIEfl FORM OF STREPTOGRAMINS, ITS PREPARATION AND PHARMACEUTICAL COMPOSITIONS CONTAINING IT Purified form of streptogramins, consisting of a combination of one or more group B components 'of streptogramins, of general formula: S *5S* 5.55 55*0.. S S S. S *5 S in which A, is a radical of general formula: 0 0 KH (CH 3 2 0 (Ia') 0 for which R' is H or OH and Y is H, a methylamino radical or a dimethylamino radical, R is an ethyl radical or, when RI is H, R can also 2/2 represent -CH 3 and and R 2 are H, or a) ternatively A, is a radical of formula: 0 0 NH 3 CHCE 3 c" 3 0 CH 3 R is an isobutyl radical, and R, is OIH and R 2 is -CH 3 and one or more group A mYinority components of streptogramins, of general formula: 00 0. 0 OH NII OH H 3 0 CH 3 0 00 *0C*C 0 N I R* 0 in which R' t is H or a methyl or ethyl radical, in the- form of a cocrystallizate, a coprecipitate or a physical mixture have useful antibacterial activity.