AU6073299A - Oligonucleotide primers that destabilize non-specific duplex formation and uses thereof - Google Patents

Oligonucleotide primers that destabilize non-specific duplex formation and uses thereof

Info

Publication number
AU6073299A
AU6073299A AU60732/99A AU6073299A AU6073299A AU 6073299 A AU6073299 A AU 6073299A AU 60732/99 A AU60732/99 A AU 60732/99A AU 6073299 A AU6073299 A AU 6073299A AU 6073299 A AU6073299 A AU 6073299A
Authority
AU
Australia
Prior art keywords
oligonucleotide primers
duplex formation
specific duplex
destabilize
destabilize non
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Abandoned
Application number
AU60732/99A
Inventor
Manjula Das
Jerry Pelletier
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
McGill University
Original Assignee
McGill University
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by McGill University filed Critical McGill University
Publication of AU6073299A publication Critical patent/AU6073299A/en
Abandoned legal-status Critical Current

Links

Classifications

    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844Nucleic acid amplification reactions
    • C12Q1/6853Nucleic acid amplification reactions using modified primers or templates
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813Hybridisation assays
    • C12Q1/6832Enhancement of hybridisation reaction
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6869Methods for sequencing

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • Biotechnology (AREA)
  • Molecular Biology (AREA)
  • Biophysics (AREA)
  • Analytical Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Immunology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
AU60732/99A 1998-10-07 1999-10-06 Oligonucleotide primers that destabilize non-specific duplex formation and uses thereof Abandoned AU6073299A (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
CA 2246623 CA2246623A1 (en) 1998-10-07 1998-10-07 Oligonucleotide primers that destabilize non-specific duplex formation and reduce mispriming during cdna library construction and uses thereof
CA2246623 1998-10-07
PCT/CA1999/000933 WO2000020630A1 (en) 1998-10-07 1999-10-06 Oligonucleotide primers that destabilize non-specific duplex formation and uses thereof

Publications (1)

Publication Number Publication Date
AU6073299A true AU6073299A (en) 2000-04-26

Family

ID=4162802

Family Applications (1)

Application Number Title Priority Date Filing Date
AU60732/99A Abandoned AU6073299A (en) 1998-10-07 1999-10-06 Oligonucleotide primers that destabilize non-specific duplex formation and uses thereof

Country Status (5)

Country Link
EP (1) EP1117826A1 (en)
JP (1) JP2002532063A (en)
AU (1) AU6073299A (en)
CA (1) CA2246623A1 (en)
WO (1) WO2000020630A1 (en)

Families Citing this family (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2002042490A1 (en) * 2000-11-24 2002-05-30 Asper OÜ Method of optimising the sequences of synthetic nucleic acids
US9261460B2 (en) * 2002-03-12 2016-02-16 Enzo Life Sciences, Inc. Real-time nucleic acid detection processes and compositions
US20030175749A1 (en) 2001-12-08 2003-09-18 Jong-Yoon Chun Annealing control primer and its uses
ES2257895B1 (en) * 2003-05-30 2008-03-16 Consejo Sup. De Invest. Cientificas OPTIMIZATION PROCEDURE FOR OLIGONUCLEOTIDES AND AMPLIFICATION CONDITIONS BY PCR FOR RACE (5 'AND 3'-RAPID AMPLIFICATION OF CDNA ENDS).
US20070212695A1 (en) * 2005-01-12 2007-09-13 Applera Corporation Compositions, methods, and kits for selective amplification of nucleic acids
JP2012000044A (en) * 2010-06-16 2012-01-05 Hitachi Ltd Method for large-scale parallel nucleic acid analysis
GB2497510A (en) * 2011-11-10 2013-06-19 Harry Cuppens Methods for determining mononucleotide sequence repeats

Family Cites Families (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5438131A (en) * 1992-09-16 1995-08-01 Bergstrom; Donald E. 3-nitropyrrole nucleoside
ES2289753T3 (en) * 1995-11-16 2008-02-01 Dako Denmark A/S IN SITU HYBRIDIZATION TO DETECT SEQUENCES OF SPECIFIC NUCLEIC ACIDS IN EUCARIOTIC SAMPLES.

Also Published As

Publication number Publication date
WO2000020630A1 (en) 2000-04-13
CA2246623A1 (en) 2000-04-07
JP2002532063A (en) 2002-10-02
EP1117826A1 (en) 2001-07-25

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Legal Events

Date Code Title Description
MK6 Application lapsed section 142(2)(f)/reg. 8.3(3) - pct applic. not entering national phase