AU5560699A - Optically characterizing polymers - Google Patents
Optically characterizing polymers Download PDFInfo
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- AU5560699A AU5560699A AU55606/99A AU5560699A AU5560699A AU 5560699 A AU5560699 A AU 5560699A AU 55606/99 A AU55606/99 A AU 55606/99A AU 5560699 A AU5560699 A AU 5560699A AU 5560699 A AU5560699 A AU 5560699A
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- polymer
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- 229920000642 polymer Polymers 0.000 title claims description 137
- 230000005855 radiation Effects 0.000 claims description 155
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- VYPSYNLAJGMNEJ-UHFFFAOYSA-N silicon dioxide Inorganic materials O=[Si]=O VYPSYNLAJGMNEJ-UHFFFAOYSA-N 0.000 description 21
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- XPPKVPWEQAFLFU-UHFFFAOYSA-J diphosphate(4-) Chemical compound [O-]P([O-])(=O)OP([O-])([O-])=O XPPKVPWEQAFLFU-UHFFFAOYSA-J 0.000 description 6
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- UQEAIHBTYFGYIE-UHFFFAOYSA-N hexamethyldisiloxane Chemical compound C[Si](C)(C)O[Si](C)(C)C UQEAIHBTYFGYIE-UHFFFAOYSA-N 0.000 description 4
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- JLCPHMBAVCMARE-UHFFFAOYSA-N [3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-[[3-[[3-[[3-[[3-[[3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-hydroxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methyl [5-(6-aminopurin-9-yl)-2-(hydroxymethyl)oxolan-3-yl] hydrogen phosphate Polymers Cc1cn(C2CC(OP(O)(=O)OCC3OC(CC3OP(O)(=O)OCC3OC(CC3O)n3cnc4c3nc(N)[nH]c4=O)n3cnc4c3nc(N)[nH]c4=O)C(COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3CO)n3cnc4c(N)ncnc34)n3ccc(N)nc3=O)n3cnc4c(N)ncnc34)n3ccc(N)nc3=O)n3ccc(N)nc3=O)n3ccc(N)nc3=O)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cc(C)c(=O)[nH]c3=O)n3cc(C)c(=O)[nH]c3=O)n3ccc(N)nc3=O)n3cc(C)c(=O)[nH]c3=O)n3cnc4c3nc(N)[nH]c4=O)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)O2)c(=O)[nH]c1=O JLCPHMBAVCMARE-UHFFFAOYSA-N 0.000 description 3
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- WGTODYJZXSJIAG-UHFFFAOYSA-N tetramethylrhodamine chloride Chemical compound [Cl-].C=12C=CC(N(C)C)=CC2=[O+]C2=CC(N(C)C)=CC=C2C=1C1=CC=CC=C1C(O)=O WGTODYJZXSJIAG-UHFFFAOYSA-N 0.000 description 1
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Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
- G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
- G01N21/64—Fluorescence; Phosphorescence
- G01N21/645—Specially adapted constructive features of fluorimeters
- G01N21/648—Specially adapted constructive features of fluorimeters using evanescent coupling or surface plasmon coupling for the excitation of fluorescence
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
- G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
- G01N21/64—Fluorescence; Phosphorescence
- G01N21/6428—Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes"
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/44—Resins; Plastics; Rubber; Leather
- G01N33/442—Resins; Plastics
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B82—NANOTECHNOLOGY
- B82Y—SPECIFIC USES OR APPLICATIONS OF NANOSTRUCTURES; MEASUREMENT OR ANALYSIS OF NANOSTRUCTURES; MANUFACTURE OR TREATMENT OF NANOSTRUCTURES
- B82Y20/00—Nanooptics, e.g. quantum optics or photonic crystals
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
- G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
- G01N21/64—Fluorescence; Phosphorescence
- G01N21/6428—Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes"
- G01N2021/6439—Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes" with indicators, stains, dyes, tags, labels, marks
- G01N2021/6441—Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes" with indicators, stains, dyes, tags, labels, marks with two or more labels
Landscapes
- Health & Medical Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Immunology (AREA)
- Physics & Mathematics (AREA)
- Life Sciences & Earth Sciences (AREA)
- General Physics & Mathematics (AREA)
- Pathology (AREA)
- Analytical Chemistry (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
- Optics & Photonics (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Engineering & Computer Science (AREA)
- Food Science & Technology (AREA)
- Medicinal Chemistry (AREA)
- Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)
- Investigating Or Analyzing Materials By The Use Of Electric Means (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Investigating Or Analyzing Non-Biological Materials By The Use Of Chemical Means (AREA)
- Investigating Or Analysing Biological Materials (AREA)
Applications Claiming Priority (5)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US9654498P | 1998-08-13 | 1998-08-13 | |
| US60096544 | 1998-08-13 | ||
| US12041499P | 1999-02-14 | 1999-02-14 | |
| US60120414 | 1999-02-14 | ||
| PCT/US1999/018438 WO2000009757A1 (fr) | 1998-08-13 | 1999-08-13 | Polymeres caracterises optiquement |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| AU5560699A true AU5560699A (en) | 2000-03-06 |
Family
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Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| AU55606/99A Abandoned AU5560699A (en) | 1998-08-13 | 1999-08-13 | Optically characterizing polymers |
Country Status (7)
| Country | Link |
|---|---|
| EP (1) | EP1105535A1 (fr) |
| JP (1) | JP2002522780A (fr) |
| CN (1) | CN1323355A (fr) |
| AU (1) | AU5560699A (fr) |
| CA (1) | CA2340228A1 (fr) |
| IL (1) | IL141310A0 (fr) |
| WO (1) | WO2000009757A1 (fr) |
Families Citing this family (71)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20120160687A1 (en) | 1995-03-17 | 2012-06-28 | President And Fellows Of Harvard College | Characterization of individual polymer molecules based on monomer-interface interactions |
| US6362002B1 (en) | 1995-03-17 | 2002-03-26 | President And Fellows Of Harvard College | Characterization of individual polymer molecules based on monomer-interface interactions |
| US8142708B2 (en) | 1995-04-03 | 2012-03-27 | Wisconsin Alumni Research Foundation | Micro fluidic system for single molecule imaging |
| US7775368B2 (en) | 1995-04-03 | 2010-08-17 | Wisconsin Alumni Research Foundation | Micro-channel long molecule manipulation system |
| IL156002A0 (en) | 1997-02-12 | 2003-12-23 | Eugene Y Chan | Methods and products for analyzing polymers |
| US6263286B1 (en) | 1998-08-13 | 2001-07-17 | U.S. Genomics, Inc. | Methods of analyzing polymers using a spatial network of fluorophores and fluorescence resonance energy transfer |
| US7056661B2 (en) | 1999-05-19 | 2006-06-06 | Cornell Research Foundation, Inc. | Method for sequencing nucleic acid molecules |
| EP1192453B1 (fr) | 1999-06-22 | 2012-02-15 | President and Fellows of Harvard College | Evaluation a l'echelle atomique et moleculaire de biopolymeres |
| CN1379857A (zh) * | 1999-08-13 | 2002-11-13 | 美国吉诺米克斯有限公司 | 用于伸展聚合物的方法和装置 |
| US6762059B2 (en) | 1999-08-13 | 2004-07-13 | U.S. Genomics, Inc. | Methods and apparatuses for characterization of single polymers |
| US6696022B1 (en) | 1999-08-13 | 2004-02-24 | U.S. Genomics, Inc. | Methods and apparatuses for stretching polymers |
| US6927065B2 (en) | 1999-08-13 | 2005-08-09 | U.S. Genomics, Inc. | Methods and apparatus for characterization of single polymers |
| AU7105300A (en) | 1999-09-03 | 2001-04-10 | Lifebeam Technologies, Inc. | Optical system for rapid polymer analysis |
| GB9923644D0 (en) | 1999-10-06 | 1999-12-08 | Medical Biosystems Ltd | DNA sequencing |
| US6908736B1 (en) | 1999-10-06 | 2005-06-21 | Medical Biosystems, Ltd. | DNA sequencing method |
| WO2001084197A1 (fr) | 2000-04-28 | 2001-11-08 | Edgelight Biosciences, Inc. | Dispositif a microreseau destine a detecter la fluorescence d'ondes evanescentes |
| JP2002048765A (ja) * | 2000-08-03 | 2002-02-15 | Masabumi Youda | 水平面型ゲル電気泳動装置用試料導入装置、水平面型ゲル電気泳動システム、その試料導入方法およびその試料採取方法 |
| WO2002044425A2 (fr) | 2000-12-01 | 2002-06-06 | Visigen Biotechnologies, Inc. | Synthese d'acides nucleiques d'enzymes, et compositions et methodes modifiant la fidelite d'incorporation de monomeres |
| EP2465943A3 (fr) | 2001-03-16 | 2012-10-03 | Kalim Mir | Affichage de polymère linéaire |
| US9678038B2 (en) | 2001-07-25 | 2017-06-13 | The Trustees Of Princeton University | Nanochannel arrays and their preparation and use for high throughput macromolecular analysis |
| CA2454570C (fr) | 2001-07-25 | 2016-12-20 | The Trustees Of Princeton University | Reseaux de nanocanaux, leurs preparation et utilisation dans l'analyse macromoleculaire a rendement eleve |
| US8423294B2 (en) | 2001-09-18 | 2013-04-16 | Pathogenetix, Inc. | High resolution linear analysis of polymers |
| JP2005289809A (ja) | 2001-10-24 | 2005-10-20 | Vlaams Interuniversitair Inst Voor Biotechnologie Vzw (Vib Vzw) | 突然変異重鎖抗体 |
| WO2003106693A2 (fr) | 2002-01-01 | 2003-12-24 | Princeton University | Structures de gradient interfaçant des elements microfluidiques et des elements nanofluidiques, leurs procedes de fabrication et d'utilisation |
| US7371520B2 (en) | 2002-05-28 | 2008-05-13 | U.S. Genomics, Inc. | Methods and apparati using single polymer analysis |
| US7282330B2 (en) | 2002-05-28 | 2007-10-16 | U.S. Genomics, Inc. | Methods and apparati using single polymer analysis |
| US20040053399A1 (en) * | 2002-07-17 | 2004-03-18 | Rudolf Gilmanshin | Methods and compositions for analyzing polymers using chimeric tags |
| WO2004035212A1 (fr) * | 2002-10-18 | 2004-04-29 | Wisconsin Alumni Research Foundation | Systeme de manipulation de molecules a chaine longue a micro-canaux |
| WO2004046709A1 (fr) * | 2002-11-20 | 2004-06-03 | Richard Fritz Sauter | Procede d'analyse de molecules pour le sequençage de molecules et spectrometre associe |
| US7141801B2 (en) * | 2002-12-26 | 2006-11-28 | Applied Precision, Llc | System and method of illuminating living cells for imaging |
| US20040214211A1 (en) * | 2003-01-23 | 2004-10-28 | U.S. Genomics, Inc. | Methods for analyzing polymer populations |
| EP1620203A2 (fr) * | 2003-04-10 | 2006-02-01 | U.S. Genomics, Inc. | Structures microfluidiques avancees |
| WO2005017025A2 (fr) | 2003-08-15 | 2005-02-24 | The President And Fellows Of Harvard College | Etude de molecules de polymere et conformations avec un nanopore |
| WO2005085849A2 (fr) * | 2003-10-24 | 2005-09-15 | U S. Genomics, Inc | Identification de risque biologique par dosage immunologique par fluorescence et par detection de molecule simple |
| US7977048B2 (en) | 2004-01-13 | 2011-07-12 | Pathogenetix, Inc. | Detection and quantification of analytes in solution using polymers |
| US7595160B2 (en) | 2004-01-13 | 2009-09-29 | U.S. Genomics, Inc. | Analyte detection using barcoded polymers |
| US7238485B2 (en) | 2004-03-23 | 2007-07-03 | President And Fellows Of Harvard College | Methods and apparatus for characterizing polynucleotides |
| US7351538B2 (en) | 2004-08-23 | 2008-04-01 | U.S. Genomics | Systems and methods for detecting and analyzing polymers |
| US7315019B2 (en) | 2004-09-17 | 2008-01-01 | Pacific Biosciences Of California, Inc. | Arrays of optical confinements and uses thereof |
| US7170050B2 (en) | 2004-09-17 | 2007-01-30 | Pacific Biosciences Of California, Inc. | Apparatus and methods for optical analysis of molecules |
| WO2006098772A2 (fr) | 2004-10-13 | 2006-09-21 | U.S. Genomics, Inc. | Systemes et procedes d'optimisation de mesures |
| US7888011B2 (en) | 2004-10-18 | 2011-02-15 | U.S. Genomics, Inc. | Methods for isolation of nucleic acids from prokaryotic spores |
| JP2006313151A (ja) * | 2005-04-07 | 2006-11-16 | Sysmex Corp | 血液分析装置、試料分析装置及びフローサイトメータ |
| CN103254309B (zh) | 2005-05-18 | 2017-09-26 | 埃博灵克斯股份有限公司 | 针对肿瘤坏死因子α的改进的纳米体TM |
| DE102005023617A1 (de) | 2005-05-21 | 2006-11-23 | Aspre Ag | Verfahren zum Mischen von Farben in einem Display |
| US9410889B2 (en) * | 2005-06-10 | 2016-08-09 | Applied Biosystem, Llc | Method and system for multiplex genetic analysis |
| SE529254C2 (sv) * | 2005-06-17 | 2007-06-12 | Aamic Ab | Optiskt testsystem |
| CN101203743B (zh) * | 2005-06-23 | 2011-04-06 | 皇家飞利浦电子股份有限公司 | 使用亚波长孔径或狭缝的发光传感器 |
| JP2009543023A (ja) * | 2005-07-18 | 2009-12-03 | コーニンクレッカ フィリップス エレクトロニクス エヌ ヴィ | マルチレイヤ基板構造を用いた発光センサ |
| EP1966593B1 (fr) * | 2005-12-20 | 2010-08-25 | Koninklijke Philips Electronics N.V. | Biocapteur avec ouvertures ayant une dimension inférieure à la limite de diffraction et composé d'une grille et d'un polariseur |
| WO2007072415A2 (fr) * | 2005-12-22 | 2007-06-28 | Koninklijke Philips Electronics N.V. | Capteur de luminescence fonctionnant en mode reflexion |
| WO2008078263A1 (fr) | 2006-12-21 | 2008-07-03 | Koninklijke Philips Electronics N.V. | Biocapteur d'ouverture à tranchées |
| EP1936340A1 (fr) * | 2006-12-21 | 2008-06-25 | Koninklijke Philips Electronics N.V. | Ouverture inferieure aux longueurs d`ondes |
| CN101765462B (zh) | 2007-03-28 | 2013-06-05 | 博纳基因技术有限公司 | 使用纳米通道阵列的大分子分析方法 |
| US8278047B2 (en) | 2007-10-01 | 2012-10-02 | Nabsys, Inc. | Biopolymer sequencing by hybridization of probes to form ternary complexes and variable range alignment |
| CA2991818C (fr) | 2008-03-28 | 2022-10-11 | Pacific Biosciences Of California, Inc. | Compositions et procedes pour le sequencage d'acide nucleique |
| US9650668B2 (en) | 2008-09-03 | 2017-05-16 | Nabsys 2.0 Llc | Use of longitudinally displaced nanoscale electrodes for voltage sensing of biomolecules and other analytes in fluidic channels |
| US8262879B2 (en) | 2008-09-03 | 2012-09-11 | Nabsys, Inc. | Devices and methods for determining the length of biopolymers and distances between probes bound thereto |
| US8882980B2 (en) | 2008-09-03 | 2014-11-11 | Nabsys, Inc. | Use of longitudinally displaced nanoscale electrodes for voltage sensing of biomolecules and other analytes in fluidic channels |
| US8828211B2 (en) | 2010-06-08 | 2014-09-09 | President And Fellows Of Harvard College | Nanopore device with graphene supported artificial lipid membrane |
| US8715933B2 (en) | 2010-09-27 | 2014-05-06 | Nabsys, Inc. | Assay methods using nicking endonucleases |
| US8859201B2 (en) | 2010-11-16 | 2014-10-14 | Nabsys, Inc. | Methods for sequencing a biomolecule by detecting relative positions of hybridized probes |
| US11274341B2 (en) | 2011-02-11 | 2022-03-15 | NABsys, 2.0 LLC | Assay methods using DNA binding proteins |
| EP2574923A1 (fr) | 2011-09-28 | 2013-04-03 | Koninklijke Philips Electronics N.V. | Appareil pour le traitement de molécules uniques |
| US9028776B2 (en) | 2012-04-18 | 2015-05-12 | Toxic Report Llc | Device for stretching a polymer in a fluid sample |
| WO2013158860A1 (fr) * | 2012-04-18 | 2013-10-24 | Pathogenetix, Inc. | Dispositifs et méthodes de préparation et d'analyse d'acides nucléiques |
| US8685708B2 (en) | 2012-04-18 | 2014-04-01 | Pathogenetix, Inc. | Device for preparing a sample |
| CN102923635B (zh) * | 2012-10-26 | 2015-06-03 | 中国科学院苏州纳米技术与纳米仿生研究所 | 纳米流体二极管及其制造方法 |
| US9914966B1 (en) | 2012-12-20 | 2018-03-13 | Nabsys 2.0 Llc | Apparatus and methods for analysis of biomolecules using high frequency alternating current excitation |
| US10294516B2 (en) | 2013-01-18 | 2019-05-21 | Nabsys 2.0 Llc | Enhanced probe binding |
| LU100392B1 (en) | 2017-07-27 | 2019-02-12 | Wisconsin Alumni Res Foundation Warf | A method to determine the throughput speed of a pore |
Family Cites Families (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US6150089A (en) * | 1988-09-15 | 2000-11-21 | New York University | Method and characterizing polymer molecules or the like |
| US5538898A (en) * | 1995-03-16 | 1996-07-23 | International Business Machines Corporation | Method suitable for identifying a code sequence of a biomolecule |
| US5624845A (en) * | 1995-03-16 | 1997-04-29 | International Business Machines Corporation | Assembly and a method suitable for identifying a code |
| WO1996030508A1 (fr) * | 1995-03-24 | 1996-10-03 | Ely Michael Rabani | Assemblage d'objets moleculaires et supramoleculaires complexes, dispositifs ainsi obtenus et leurs utilisations |
| AU5532196A (en) * | 1995-04-03 | 1996-10-23 | New York University | Methods for measuring physical characteristics of nucleic ac ids by microscopic imaging |
| US5699157A (en) * | 1996-07-16 | 1997-12-16 | Caliper Technologies Corp. | Fourier detection of species migrating in a microchannel |
| IL156002A0 (en) * | 1997-02-12 | 2003-12-23 | Eugene Y Chan | Methods and products for analyzing polymers |
-
1999
- 1999-08-13 WO PCT/US1999/018438 patent/WO2000009757A1/fr active Application Filing
- 1999-08-13 CN CN99811963A patent/CN1323355A/zh active Pending
- 1999-08-13 AU AU55606/99A patent/AU5560699A/en not_active Abandoned
- 1999-08-13 CA CA002340228A patent/CA2340228A1/fr not_active Abandoned
- 1999-08-13 JP JP2000565191A patent/JP2002522780A/ja active Pending
- 1999-08-13 EP EP99942167A patent/EP1105535A1/fr not_active Withdrawn
- 1999-08-13 IL IL14131099A patent/IL141310A0/xx unknown
Also Published As
| Publication number | Publication date |
|---|---|
| WO2000009757A9 (fr) | 2000-10-12 |
| WO2000009757A1 (fr) | 2000-02-24 |
| IL141310A0 (en) | 2002-03-10 |
| JP2002522780A (ja) | 2002-07-23 |
| CA2340228A1 (fr) | 2000-02-24 |
| EP1105535A1 (fr) | 2001-06-13 |
| CN1323355A (zh) | 2001-11-21 |
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