AU2038283A - The use of eucaryotic promoter sequences in the production ofproteinaceous materials - Google Patents
The use of eucaryotic promoter sequences in the production ofproteinaceous materialsInfo
- Publication number
- AU2038283A AU2038283A AU20382/83A AU2038283A AU2038283A AU 2038283 A AU2038283 A AU 2038283A AU 20382/83 A AU20382/83 A AU 20382/83A AU 2038283 A AU2038283 A AU 2038283A AU 2038283 A AU2038283 A AU 2038283A
- Authority
- AU
- Australia
- Prior art keywords
- dna
- gene
- cells
- accordance
- cell
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Abandoned
Links
- 239000000463 material Substances 0.000 title claims description 55
- 238000004519 manufacturing process Methods 0.000 title claims description 7
- 210000004027 cell Anatomy 0.000 claims description 513
- 108020004414 DNA Proteins 0.000 claims description 397
- 108090000623 proteins and genes Proteins 0.000 claims description 288
- 238000000034 method Methods 0.000 claims description 117
- 108060003196 globin Proteins 0.000 claims description 89
- 108010000521 Human Growth Hormone Proteins 0.000 claims description 84
- 230000008569 process Effects 0.000 claims description 81
- 108020004999 messenger RNA Proteins 0.000 claims description 67
- 102000002265 Human Growth Hormone Human genes 0.000 claims description 61
- 239000000854 Human Growth Hormone Substances 0.000 claims description 61
- 102000018146 globin Human genes 0.000 claims description 57
- 239000013612 plasmid Substances 0.000 claims description 52
- 230000001939 inductive effect Effects 0.000 claims description 44
- 102000004190 Enzymes Human genes 0.000 claims description 43
- 108090000790 Enzymes Proteins 0.000 claims description 43
- FBOZXECLQNJBKD-ZDUSSCGKSA-N L-methotrexate Chemical compound C=1N=C2N=C(N)N=C(N)C2=NC=1CN(C)C1=CC=C(C(=O)N[C@@H](CCC(O)=O)C(O)=O)C=C1 FBOZXECLQNJBKD-ZDUSSCGKSA-N 0.000 claims description 38
- 229960000485 methotrexate Drugs 0.000 claims description 38
- 210000002950 fibroblast Anatomy 0.000 claims description 32
- 102000004169 proteins and genes Human genes 0.000 claims description 31
- NOESYZHRGYRDHS-UHFFFAOYSA-N insulin Chemical compound N1C(=O)C(NC(=O)C(CCC(N)=O)NC(=O)C(CCC(O)=O)NC(=O)C(C(C)C)NC(=O)C(NC(=O)CN)C(C)CC)CSSCC(C(NC(CO)C(=O)NC(CC(C)C)C(=O)NC(CC=2C=CC(O)=CC=2)C(=O)NC(CCC(N)=O)C(=O)NC(CC(C)C)C(=O)NC(CCC(O)=O)C(=O)NC(CC(N)=O)C(=O)NC(CC=2C=CC(O)=CC=2)C(=O)NC(CSSCC(NC(=O)C(C(C)C)NC(=O)C(CC(C)C)NC(=O)C(CC=2C=CC(O)=CC=2)NC(=O)C(CC(C)C)NC(=O)C(C)NC(=O)C(CCC(O)=O)NC(=O)C(C(C)C)NC(=O)C(CC(C)C)NC(=O)C(CC=2NC=NC=2)NC(=O)C(CO)NC(=O)CNC2=O)C(=O)NCC(=O)NC(CCC(O)=O)C(=O)NC(CCCNC(N)=N)C(=O)NCC(=O)NC(CC=3C=CC=CC=3)C(=O)NC(CC=3C=CC=CC=3)C(=O)NC(CC=3C=CC(O)=CC=3)C(=O)NC(C(C)O)C(=O)N3C(CCC3)C(=O)NC(CCCCN)C(=O)NC(C)C(O)=O)C(=O)NC(CC(N)=O)C(O)=O)=O)NC(=O)C(C(C)CC)NC(=O)C(CO)NC(=O)C(C(C)O)NC(=O)C1CSSCC2NC(=O)C(CC(C)C)NC(=O)C(NC(=O)C(CCC(N)=O)NC(=O)C(CC(N)=O)NC(=O)C(NC(=O)C(N)CC=1C=CC=CC=1)C(C)C)CC1=CN=CN1 NOESYZHRGYRDHS-UHFFFAOYSA-N 0.000 claims description 26
- 230000003612 virological effect Effects 0.000 claims description 25
- 102000014150 Interferons Human genes 0.000 claims description 24
- 108010050904 Interferons Proteins 0.000 claims description 24
- 108020004440 Thymidine kinase Proteins 0.000 claims description 21
- 230000001105 regulatory effect Effects 0.000 claims description 19
- 241000700584 Simplexvirus Species 0.000 claims description 16
- 239000003795 chemical substances by application Substances 0.000 claims description 16
- 229940079322 interferon Drugs 0.000 claims description 16
- IAZDPXIOMUYVGZ-UHFFFAOYSA-N Dimethylsulphoxide Chemical compound CS(C)=O IAZDPXIOMUYVGZ-UHFFFAOYSA-N 0.000 claims description 15
- 230000015572 biosynthetic process Effects 0.000 claims description 15
- 230000002950 deficient Effects 0.000 claims description 14
- 210000004962 mammalian cell Anatomy 0.000 claims description 14
- 108090001061 Insulin Proteins 0.000 claims description 13
- 102000004877 Insulin Human genes 0.000 claims description 13
- 102000006601 Thymidine Kinase Human genes 0.000 claims description 13
- 229940125396 insulin Drugs 0.000 claims description 13
- 102000018997 Growth Hormone Human genes 0.000 claims description 12
- 108010051696 Growth Hormone Proteins 0.000 claims description 12
- 230000001580 bacterial effect Effects 0.000 claims description 12
- 238000010367 cloning Methods 0.000 claims description 12
- 239000000122 growth hormone Substances 0.000 claims description 12
- 230000004083 survival effect Effects 0.000 claims description 12
- 206010059866 Drug resistance Diseases 0.000 claims description 11
- 229940079593 drug Drugs 0.000 claims description 11
- 239000003814 drug Substances 0.000 claims description 11
- 239000012620 biological material Substances 0.000 claims description 10
- 102000053602 DNA Human genes 0.000 claims description 9
- 239000013611 chromosomal DNA Substances 0.000 claims description 9
- 238000003786 synthesis reaction Methods 0.000 claims description 9
- 108010022394 Threonine synthase Proteins 0.000 claims description 8
- AUYYCJSJGJYCDS-LBPRGKRZSA-N Thyrolar Chemical class IC1=CC(C[C@H](N)C(O)=O)=CC(I)=C1OC1=CC=C(O)C(I)=C1 AUYYCJSJGJYCDS-LBPRGKRZSA-N 0.000 claims description 8
- 239000001506 calcium phosphate Substances 0.000 claims description 8
- 229910000389 calcium phosphate Inorganic materials 0.000 claims description 8
- 235000011010 calcium phosphates Nutrition 0.000 claims description 8
- 210000003924 normoblast Anatomy 0.000 claims description 8
- QORWJWZARLRLPR-UHFFFAOYSA-H tricalcium bis(phosphate) Chemical compound [Ca+2].[Ca+2].[Ca+2].[O-]P([O-])([O-])=O.[O-]P([O-])([O-])=O QORWJWZARLRLPR-UHFFFAOYSA-H 0.000 claims description 8
- 229940036555 thyroid hormone Drugs 0.000 claims description 7
- 239000005495 thyroid hormone Substances 0.000 claims description 7
- 102000015081 Blood Coagulation Factors Human genes 0.000 claims description 6
- 108010039209 Blood Coagulation Factors Proteins 0.000 claims description 6
- 239000003114 blood coagulation factor Substances 0.000 claims description 6
- 239000000427 antigen Substances 0.000 claims description 5
- 108091007433 antigens Proteins 0.000 claims description 5
- 102000036639 antigens Human genes 0.000 claims description 5
- 239000003246 corticosteroid Substances 0.000 claims description 5
- 102000004419 dihydrofolate reductase Human genes 0.000 claims description 5
- 108010024223 Adenine phosphoribosyltransferase Proteins 0.000 claims description 3
- 102100029457 Adenine phosphoribosyltransferase Human genes 0.000 claims description 3
- 241001465754 Metazoa Species 0.000 claims description 3
- 239000005557 antagonist Substances 0.000 claims description 3
- 230000000295 complement effect Effects 0.000 claims description 3
- 208000007056 sickle cell anemia Diseases 0.000 claims description 3
- 150000001875 compounds Chemical class 0.000 claims description 2
- 239000002245 particle Substances 0.000 claims description 2
- 238000011084 recovery Methods 0.000 claims description 2
- 230000001225 therapeutic effect Effects 0.000 claims description 2
- 239000008194 pharmaceutical composition Substances 0.000 claims 4
- 238000012258 culturing Methods 0.000 claims 3
- 108010006025 bovine growth hormone Proteins 0.000 claims 2
- 210000003743 erythrocyte Anatomy 0.000 claims 1
- 230000009395 genetic defect Effects 0.000 claims 1
- 150000003462 sulfoxides Chemical class 0.000 claims 1
- 239000012634 fragment Substances 0.000 description 110
- 241000699666 Mus <mouse, genus> Species 0.000 description 105
- 241000283973 Oryctolagus cuniculus Species 0.000 description 104
- 230000009466 transformation Effects 0.000 description 92
- 108091032973 (ribonucleotides)n+m Proteins 0.000 description 88
- 238000002474 experimental method Methods 0.000 description 68
- 238000009396 hybridization Methods 0.000 description 59
- 208000001608 teratocarcinoma Diseases 0.000 description 56
- 108091005904 Hemoglobin subunit beta Proteins 0.000 description 52
- 230000014509 gene expression Effects 0.000 description 50
- 101150003725 TK gene Proteins 0.000 description 44
- 101150074155 DHFR gene Proteins 0.000 description 37
- 230000006698 induction Effects 0.000 description 37
- 238000012546 transfer Methods 0.000 description 31
- 101150094949 APRT gene Proteins 0.000 description 29
- 238000003776 cleavage reaction Methods 0.000 description 29
- 230000010354 integration Effects 0.000 description 29
- 230000007017 scission Effects 0.000 description 29
- 230000003321 amplification Effects 0.000 description 27
- 238000003199 nucleic acid amplification method Methods 0.000 description 27
- 238000000137 annealing Methods 0.000 description 26
- 230000000694 effects Effects 0.000 description 26
- 108010054576 Deoxyribonuclease EcoRI Proteins 0.000 description 25
- 102100021519 Hemoglobin subunit beta Human genes 0.000 description 25
- 206010028980 Neoplasm Diseases 0.000 description 24
- 239000002609 medium Substances 0.000 description 24
- 238000004458 analytical method Methods 0.000 description 23
- 108091028043 Nucleic acid sequence Proteins 0.000 description 21
- 239000002773 nucleotide Substances 0.000 description 21
- 125000003729 nucleotide group Chemical group 0.000 description 21
- 239000000523 sample Substances 0.000 description 21
- 239000002299 complementary DNA Substances 0.000 description 19
- 241000894007 species Species 0.000 description 19
- 241000699800 Cricetinae Species 0.000 description 18
- 108091092356 cellular DNA Proteins 0.000 description 18
- 230000002068 genetic effect Effects 0.000 description 18
- 239000003862 glucocorticoid Substances 0.000 description 18
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 description 17
- 230000001413 cellular effect Effects 0.000 description 16
- 230000004927 fusion Effects 0.000 description 16
- 229940088597 hormone Drugs 0.000 description 16
- 239000005556 hormone Substances 0.000 description 16
- 238000013518 transcription Methods 0.000 description 16
- 230000035897 transcription Effects 0.000 description 16
- 238000007792 addition Methods 0.000 description 15
- 238000013459 approach Methods 0.000 description 14
- 230000001086 cytosolic effect Effects 0.000 description 14
- 239000003550 marker Substances 0.000 description 14
- 108091008146 restriction endonucleases Proteins 0.000 description 14
- 108091092195 Intron Proteins 0.000 description 13
- 210000004748 cultured cell Anatomy 0.000 description 13
- 230000001965 increasing effect Effects 0.000 description 13
- 239000013598 vector Substances 0.000 description 13
- 241001529936 Murinae Species 0.000 description 12
- IQFYYKKMVGJFEH-XLPZGREQSA-N Thymidine Chemical compound O=C1NC(=O)C(C)=CN1[C@@H]1O[C@H](CO)[C@@H](O)C1 IQFYYKKMVGJFEH-XLPZGREQSA-N 0.000 description 12
- 239000000499 gel Substances 0.000 description 12
- 230000001817 pituitary effect Effects 0.000 description 12
- 238000006243 chemical reaction Methods 0.000 description 11
- 210000000805 cytoplasm Anatomy 0.000 description 11
- 210000002966 serum Anatomy 0.000 description 11
- KCXVZYZYPLLWCC-UHFFFAOYSA-N EDTA Chemical compound OC(=O)CN(CC(O)=O)CCN(CC(O)=O)CC(O)=O KCXVZYZYPLLWCC-UHFFFAOYSA-N 0.000 description 10
- 241000287828 Gallus gallus Species 0.000 description 10
- 101000899111 Homo sapiens Hemoglobin subunit beta Proteins 0.000 description 10
- 238000000636 Northern blotting Methods 0.000 description 10
- 235000013330 chicken meat Nutrition 0.000 description 10
- 230000003054 hormonal effect Effects 0.000 description 10
- FDGQSTZJBFJUBT-UHFFFAOYSA-N hypoxanthine Chemical compound O=C1NC=NC2=C1NC=N2 FDGQSTZJBFJUBT-UHFFFAOYSA-N 0.000 description 10
- 238000001727 in vivo Methods 0.000 description 10
- 239000000047 product Substances 0.000 description 10
- 239000011780 sodium chloride Substances 0.000 description 10
- 239000000020 Nitrocellulose Substances 0.000 description 9
- 238000003556 assay Methods 0.000 description 9
- 230000002759 chromosomal effect Effects 0.000 description 9
- 230000004069 differentiation Effects 0.000 description 9
- 230000006870 function Effects 0.000 description 9
- 239000001963 growth medium Substances 0.000 description 9
- 230000001404 mediated effect Effects 0.000 description 9
- 229920001220 nitrocellulos Polymers 0.000 description 9
- 238000002360 preparation method Methods 0.000 description 9
- 238000012545 processing Methods 0.000 description 9
- 239000006152 selective media Substances 0.000 description 9
- 210000000349 chromosome Anatomy 0.000 description 8
- 210000004940 nucleus Anatomy 0.000 description 8
- 238000000844 transformation Methods 0.000 description 8
- 108091092328 cellular RNA Proteins 0.000 description 7
- 238000005755 formation reaction Methods 0.000 description 7
- 238000002955 isolation Methods 0.000 description 7
- 239000002244 precipitate Substances 0.000 description 7
- 239000002243 precursor Substances 0.000 description 7
- 239000000243 solution Substances 0.000 description 7
- 238000012360 testing method Methods 0.000 description 7
- 210000001519 tissue Anatomy 0.000 description 7
- DWRXFEITVBNRMK-UHFFFAOYSA-N Beta-D-1-Arabinofuranosylthymine Natural products O=C1NC(=O)C(C)=CN1C1C(O)C(O)C(CO)O1 DWRXFEITVBNRMK-UHFFFAOYSA-N 0.000 description 6
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 description 6
- ZHNUHDYFZUAESO-UHFFFAOYSA-N Formamide Chemical compound NC=O ZHNUHDYFZUAESO-UHFFFAOYSA-N 0.000 description 6
- 108091034057 RNA (poly(A)) Proteins 0.000 description 6
- 239000007983 Tris buffer Substances 0.000 description 6
- 239000011543 agarose gel Substances 0.000 description 6
- 150000001413 amino acids Chemical group 0.000 description 6
- IQFYYKKMVGJFEH-UHFFFAOYSA-N beta-L-thymidine Natural products O=C1NC(=O)C(C)=CN1C1OC(CO)C(O)C1 IQFYYKKMVGJFEH-UHFFFAOYSA-N 0.000 description 6
- 244000309466 calf Species 0.000 description 6
- 108091092330 cytoplasmic RNA Proteins 0.000 description 6
- 238000011161 development Methods 0.000 description 6
- 230000018109 developmental process Effects 0.000 description 6
- 239000000284 extract Substances 0.000 description 6
- 238000013383 initial experiment Methods 0.000 description 6
- 230000002285 radioactive effect Effects 0.000 description 6
- 230000002829 reductive effect Effects 0.000 description 6
- 210000000130 stem cell Anatomy 0.000 description 6
- 229940104230 thymidine Drugs 0.000 description 6
- 230000001131 transforming effect Effects 0.000 description 6
- LENZDBCJOHFCAS-UHFFFAOYSA-N tris Chemical compound OCC(N)(CO)CO LENZDBCJOHFCAS-UHFFFAOYSA-N 0.000 description 6
- 241000894006 Bacteria Species 0.000 description 5
- 241000588724 Escherichia coli Species 0.000 description 5
- UGQMRVRMYYASKQ-UHFFFAOYSA-N Hypoxanthine nucleoside Natural products OC1C(O)C(CO)OC1N1C(NC=NC2=O)=C2N=C1 UGQMRVRMYYASKQ-UHFFFAOYSA-N 0.000 description 5
- 239000004098 Tetracycline Substances 0.000 description 5
- 108700005077 Viral Genes Proteins 0.000 description 5
- 230000009471 action Effects 0.000 description 5
- 238000000246 agarose gel electrophoresis Methods 0.000 description 5
- 229960000723 ampicillin Drugs 0.000 description 5
- AVKUERGKIZMTKX-NJBDSQKTSA-N ampicillin Chemical compound C1([C@@H](N)C(=O)N[C@H]2[C@H]3SC([C@@H](N3C2=O)C(O)=O)(C)C)=CC=CC=C1 AVKUERGKIZMTKX-NJBDSQKTSA-N 0.000 description 5
- 238000004113 cell culture Methods 0.000 description 5
- 229920002678 cellulose Polymers 0.000 description 5
- 239000001913 cellulose Substances 0.000 description 5
- 230000002255 enzymatic effect Effects 0.000 description 5
- 230000000977 initiatory effect Effects 0.000 description 5
- 239000000543 intermediate Substances 0.000 description 5
- 238000006386 neutralization reaction Methods 0.000 description 5
- 230000003362 replicative effect Effects 0.000 description 5
- 235000000346 sugar Nutrition 0.000 description 5
- 239000006228 supernatant Substances 0.000 description 5
- 229960002180 tetracycline Drugs 0.000 description 5
- 229930101283 tetracycline Natural products 0.000 description 5
- 235000019364 tetracycline Nutrition 0.000 description 5
- 150000003522 tetracyclines Chemical class 0.000 description 5
- 241001515965 unidentified phage Species 0.000 description 5
- 102000040650 (ribonucleotides)n+m Human genes 0.000 description 4
- TVZGACDUOSZQKY-LBPRGKRZSA-N 4-aminofolic acid Chemical compound C1=NC2=NC(N)=NC(N)=C2N=C1CNC1=CC=C(C(=O)N[C@@H](CCC(O)=O)C(O)=O)C=C1 TVZGACDUOSZQKY-LBPRGKRZSA-N 0.000 description 4
- UXVMQQNJUSDDNG-UHFFFAOYSA-L Calcium chloride Chemical compound [Cl-].[Cl-].[Ca+2] UXVMQQNJUSDDNG-UHFFFAOYSA-L 0.000 description 4
- KRKNYBCHXYNGOX-UHFFFAOYSA-K Citrate Chemical compound [O-]C(=O)CC(O)(CC([O-])=O)C([O-])=O KRKNYBCHXYNGOX-UHFFFAOYSA-K 0.000 description 4
- 102000003886 Glycoproteins Human genes 0.000 description 4
- 108090000288 Glycoproteins Proteins 0.000 description 4
- 102000003960 Ligases Human genes 0.000 description 4
- 108090000364 Ligases Proteins 0.000 description 4
- TWRXJAOTZQYOKJ-UHFFFAOYSA-L Magnesium chloride Chemical compound [Mg+2].[Cl-].[Cl-] TWRXJAOTZQYOKJ-UHFFFAOYSA-L 0.000 description 4
- 101100278853 Mus musculus Dhfr gene Proteins 0.000 description 4
- 241000699670 Mus sp. Species 0.000 description 4
- 108091093105 Nuclear DNA Proteins 0.000 description 4
- 101710163270 Nuclease Proteins 0.000 description 4
- 108020004511 Recombinant DNA Proteins 0.000 description 4
- 230000001594 aberrant effect Effects 0.000 description 4
- 108010018360 alpha 2u globulin Proteins 0.000 description 4
- 229960003896 aminopterin Drugs 0.000 description 4
- 230000008901 benefit Effects 0.000 description 4
- 239000000872 buffer Substances 0.000 description 4
- 239000001110 calcium chloride Substances 0.000 description 4
- 229910001628 calcium chloride Inorganic materials 0.000 description 4
- 238000005119 centrifugation Methods 0.000 description 4
- 238000012512 characterization method Methods 0.000 description 4
- 230000001276 controlling effect Effects 0.000 description 4
- 229960003957 dexamethasone Drugs 0.000 description 4
- UREBDLICKHMUKA-CXSFZGCWSA-N dexamethasone Chemical compound C1CC2=CC(=O)C=C[C@]2(C)[C@]2(F)[C@@H]1[C@@H]1C[C@@H](C)[C@@](C(=O)CO)(O)[C@@]1(C)C[C@@H]2O UREBDLICKHMUKA-CXSFZGCWSA-N 0.000 description 4
- 230000029087 digestion Effects 0.000 description 4
- 238000001962 electrophoresis Methods 0.000 description 4
- 210000000267 erythroid cell Anatomy 0.000 description 4
- 238000000338 in vitro Methods 0.000 description 4
- 230000003993 interaction Effects 0.000 description 4
- 210000004185 liver Anatomy 0.000 description 4
- 210000001161 mammalian embryo Anatomy 0.000 description 4
- 239000000203 mixture Substances 0.000 description 4
- 230000007935 neutral effect Effects 0.000 description 4
- 230000000717 retained effect Effects 0.000 description 4
- 230000002194 synthesizing effect Effects 0.000 description 4
- 230000014616 translation Effects 0.000 description 4
- 239000013603 viral vector Substances 0.000 description 4
- WOVKYSAHUYNSMH-RRKCRQDMSA-N 5-bromodeoxyuridine Chemical compound C1[C@H](O)[C@@H](CO)O[C@H]1N1C(=O)NC(=O)C(Br)=C1 WOVKYSAHUYNSMH-RRKCRQDMSA-N 0.000 description 3
- VKKXEIQIGGPMHT-UHFFFAOYSA-N 7h-purine-2,8-diamine Chemical compound NC1=NC=C2NC(N)=NC2=N1 VKKXEIQIGGPMHT-UHFFFAOYSA-N 0.000 description 3
- 229920000936 Agarose Polymers 0.000 description 3
- 101100263837 Bovine ephemeral fever virus (strain BB7721) beta gene Proteins 0.000 description 3
- 101100148606 Caenorhabditis elegans pst-1 gene Proteins 0.000 description 3
- 108020004635 Complementary DNA Proteins 0.000 description 3
- 239000003298 DNA probe Substances 0.000 description 3
- 108090000204 Dipeptidase 1 Proteins 0.000 description 3
- 101100316840 Enterobacteria phage P4 Beta gene Proteins 0.000 description 3
- WSFSSNUMVMOOMR-UHFFFAOYSA-N Formaldehyde Chemical compound O=C WSFSSNUMVMOOMR-UHFFFAOYSA-N 0.000 description 3
- 102000003676 Glucocorticoid Receptors Human genes 0.000 description 3
- 108090000079 Glucocorticoid Receptors Proteins 0.000 description 3
- XQFRJNBWHJMXHO-RRKCRQDMSA-N IDUR Chemical compound C1[C@H](O)[C@@H](CO)O[C@H]1N1C(=O)NC(=O)C(I)=C1 XQFRJNBWHJMXHO-RRKCRQDMSA-N 0.000 description 3
- 108020003217 Nuclear RNA Proteins 0.000 description 3
- 102000043141 Nuclear RNA Human genes 0.000 description 3
- DBMJMQXJHONAFJ-UHFFFAOYSA-M Sodium laurylsulphate Chemical compound [Na+].CCCCCCCCCCCCOS([O-])(=O)=O DBMJMQXJHONAFJ-UHFFFAOYSA-M 0.000 description 3
- 238000002105 Southern blotting Methods 0.000 description 3
- 210000004102 animal cell Anatomy 0.000 description 3
- 210000004978 chinese hamster ovary cell Anatomy 0.000 description 3
- 238000012217 deletion Methods 0.000 description 3
- 230000037430 deletion Effects 0.000 description 3
- 238000005516 engineering process Methods 0.000 description 3
- 238000002825 functional assay Methods 0.000 description 3
- 238000003780 insertion Methods 0.000 description 3
- 230000037431 insertion Effects 0.000 description 3
- 238000012423 maintenance Methods 0.000 description 3
- 230000014759 maintenance of location Effects 0.000 description 3
- 230000035772 mutation Effects 0.000 description 3
- 230000008520 organization Effects 0.000 description 3
- 229920003023 plastic Polymers 0.000 description 3
- 230000029279 positive regulation of transcription, DNA-dependent Effects 0.000 description 3
- 108010066381 preproinsulin Proteins 0.000 description 3
- 102000005962 receptors Human genes 0.000 description 3
- 108020003175 receptors Proteins 0.000 description 3
- 238000009877 rendering Methods 0.000 description 3
- 230000004044 response Effects 0.000 description 3
- 229910000162 sodium phosphate Inorganic materials 0.000 description 3
- 210000001082 somatic cell Anatomy 0.000 description 3
- 239000000126 substance Substances 0.000 description 3
- 229940037128 systemic glucocorticoids Drugs 0.000 description 3
- 238000013519 translation Methods 0.000 description 3
- DGVVWUTYPXICAM-UHFFFAOYSA-N β‐Mercaptoethanol Chemical compound OCCS DGVVWUTYPXICAM-UHFFFAOYSA-N 0.000 description 3
- JKMHFZQWWAIEOD-UHFFFAOYSA-N 2-[4-(2-hydroxyethyl)piperazin-1-yl]ethanesulfonic acid Chemical compound OCC[NH+]1CCN(CCS([O-])(=O)=O)CC1 JKMHFZQWWAIEOD-UHFFFAOYSA-N 0.000 description 2
- 108020005029 5' Flanking Region Proteins 0.000 description 2
- GFFGJBXGBJISGV-UHFFFAOYSA-N Adenine Chemical compound NC1=NC=NC2=C1N=CN2 GFFGJBXGBJISGV-UHFFFAOYSA-N 0.000 description 2
- 229930024421 Adenine Natural products 0.000 description 2
- 241000972773 Aulopiformes Species 0.000 description 2
- 108091003079 Bovine Serum Albumin Proteins 0.000 description 2
- 241000282693 Cercopithecidae Species 0.000 description 2
- HEDRZPFGACZZDS-UHFFFAOYSA-N Chloroform Chemical compound ClC(Cl)Cl HEDRZPFGACZZDS-UHFFFAOYSA-N 0.000 description 2
- 108020004638 Circular DNA Proteins 0.000 description 2
- 241000699802 Cricetulus griseus Species 0.000 description 2
- 108020003215 DNA Probes Proteins 0.000 description 2
- 230000004544 DNA amplification Effects 0.000 description 2
- 102100024746 Dihydrofolate reductase Human genes 0.000 description 2
- 239000006144 Dulbecco’s modified Eagle's medium Substances 0.000 description 2
- 241000196324 Embryophyta Species 0.000 description 2
- 208000031637 Erythroblastic Acute Leukemia Diseases 0.000 description 2
- 208000036566 Erythroleukaemia Diseases 0.000 description 2
- 241000206602 Eukaryota Species 0.000 description 2
- 108700024394 Exon Proteins 0.000 description 2
- 108091005902 Hemoglobin subunit alpha Proteins 0.000 description 2
- 102100027685 Hemoglobin subunit alpha Human genes 0.000 description 2
- 208000009889 Herpes Simplex Diseases 0.000 description 2
- 108010033040 Histones Proteins 0.000 description 2
- 241000700588 Human alphaherpesvirus 1 Species 0.000 description 2
- 108010091358 Hypoxanthine Phosphoribosyltransferase Proteins 0.000 description 2
- 102000018251 Hypoxanthine Phosphoribosyltransferase Human genes 0.000 description 2
- 108091026898 Leader sequence (mRNA) Proteins 0.000 description 2
- 101001075372 Mus musculus Gamma-glutamyl hydrolase Proteins 0.000 description 2
- 101100021974 Mus musculus Ltk gene Proteins 0.000 description 2
- 108090000854 Oxidoreductases Proteins 0.000 description 2
- 108010092799 RNA-directed DNA polymerase Proteins 0.000 description 2
- 108020005091 Replication Origin Proteins 0.000 description 2
- 239000002253 acid Substances 0.000 description 2
- 150000007513 acids Chemical class 0.000 description 2
- 230000004913 activation Effects 0.000 description 2
- 208000021841 acute erythroid leukemia Diseases 0.000 description 2
- 229960000643 adenine Drugs 0.000 description 2
- 230000003466 anti-cipated effect Effects 0.000 description 2
- 102000006635 beta-lactamase Human genes 0.000 description 2
- 238000010256 biochemical assay Methods 0.000 description 2
- 230000033228 biological regulation Effects 0.000 description 2
- 210000002459 blastocyst Anatomy 0.000 description 2
- AIYUHDOJVYHVIT-UHFFFAOYSA-M caesium chloride Chemical compound [Cl-].[Cs+] AIYUHDOJVYHVIT-UHFFFAOYSA-M 0.000 description 2
- FLJPGEWQYJVDPF-UHFFFAOYSA-L caesium sulfate Chemical compound [Cs+].[Cs+].[O-]S([O-])(=O)=O FLJPGEWQYJVDPF-UHFFFAOYSA-L 0.000 description 2
- 230000008859 change Effects 0.000 description 2
- 238000004587 chromatography analysis Methods 0.000 description 2
- 239000013599 cloning vector Substances 0.000 description 2
- 238000007796 conventional method Methods 0.000 description 2
- 230000007423 decrease Effects 0.000 description 2
- 238000001514 detection method Methods 0.000 description 2
- 238000010586 diagram Methods 0.000 description 2
- 108020001096 dihydrofolate reductase Proteins 0.000 description 2
- 230000013020 embryo development Effects 0.000 description 2
- 239000002158 endotoxin Substances 0.000 description 2
- 238000000605 extraction Methods 0.000 description 2
- 238000005194 fractionation Methods 0.000 description 2
- 238000001502 gel electrophoresis Methods 0.000 description 2
- 108091008039 hormone receptors Proteins 0.000 description 2
- 238000011534 incubation Methods 0.000 description 2
- 238000011081 inoculation Methods 0.000 description 2
- 230000004807 localization Effects 0.000 description 2
- 229910001629 magnesium chloride Inorganic materials 0.000 description 2
- 238000005259 measurement Methods 0.000 description 2
- 230000007246 mechanism Effects 0.000 description 2
- 230000021121 meiosis Effects 0.000 description 2
- 230000002503 metabolic effect Effects 0.000 description 2
- 238000002156 mixing Methods 0.000 description 2
- 230000004048 modification Effects 0.000 description 2
- 238000012986 modification Methods 0.000 description 2
- 230000001613 neoplastic effect Effects 0.000 description 2
- 230000036961 partial effect Effects 0.000 description 2
- 239000008188 pellet Substances 0.000 description 2
- 239000002953 phosphate buffered saline Substances 0.000 description 2
- 229920002401 polyacrylamide Polymers 0.000 description 2
- 238000000746 purification Methods 0.000 description 2
- 239000011541 reaction mixture Substances 0.000 description 2
- 230000008707 rearrangement Effects 0.000 description 2
- 230000006798 recombination Effects 0.000 description 2
- 238000005215 recombination Methods 0.000 description 2
- 238000011160 research Methods 0.000 description 2
- 235000019515 salmon Nutrition 0.000 description 2
- 238000012216 screening Methods 0.000 description 2
- 230000035945 sensitivity Effects 0.000 description 2
- 239000001488 sodium phosphate Substances 0.000 description 2
- 230000002269 spontaneous effect Effects 0.000 description 2
- 239000003270 steroid hormone Substances 0.000 description 2
- 102000005969 steroid hormone receptors Human genes 0.000 description 2
- 238000007920 subcutaneous administration Methods 0.000 description 2
- 239000000725 suspension Substances 0.000 description 2
- 230000001052 transient effect Effects 0.000 description 2
- 230000005945 translocation Effects 0.000 description 2
- RYFMWSXOAZQYPI-UHFFFAOYSA-K trisodium phosphate Chemical compound [Na+].[Na+].[Na+].[O-]P([O-])([O-])=O RYFMWSXOAZQYPI-UHFFFAOYSA-K 0.000 description 2
- DIGQNXIGRZPYDK-WKSCXVIASA-N (2R)-6-amino-2-[[2-[[(2S)-2-[[2-[[(2R)-2-[[(2S)-2-[[(2R,3S)-2-[[2-[[(2S)-2-[[2-[[(2S)-2-[[(2S)-2-[[(2R)-2-[[(2S,3S)-2-[[(2R)-2-[[(2S)-2-[[(2S)-2-[[(2S)-2-[[2-[[(2S)-2-[[(2R)-2-[[2-[[2-[[2-[(2-amino-1-hydroxyethylidene)amino]-3-carboxy-1-hydroxypropylidene]amino]-1-hydroxy-3-sulfanylpropylidene]amino]-1-hydroxyethylidene]amino]-1-hydroxy-3-sulfanylpropylidene]amino]-1,3-dihydroxypropylidene]amino]-1-hydroxyethylidene]amino]-1-hydroxypropylidene]amino]-1,3-dihydroxypropylidene]amino]-1,3-dihydroxypropylidene]amino]-1-hydroxy-3-sulfanylpropylidene]amino]-1,3-dihydroxybutylidene]amino]-1-hydroxy-3-sulfanylpropylidene]amino]-1-hydroxypropylidene]amino]-1,3-dihydroxypropylidene]amino]-1-hydroxyethylidene]amino]-1,5-dihydroxy-5-iminopentylidene]amino]-1-hydroxy-3-sulfanylpropylidene]amino]-1,3-dihydroxybutylidene]amino]-1-hydroxy-3-sulfanylpropylidene]amino]-1,3-dihydroxypropylidene]amino]-1-hydroxyethylidene]amino]-1-hydroxy-3-sulfanylpropylidene]amino]-1-hydroxyethylidene]amino]hexanoic acid Chemical compound C[C@@H]([C@@H](C(=N[C@@H](CS)C(=N[C@@H](C)C(=N[C@@H](CO)C(=NCC(=N[C@@H](CCC(=N)O)C(=NC(CS)C(=N[C@H]([C@H](C)O)C(=N[C@H](CS)C(=N[C@H](CO)C(=NCC(=N[C@H](CS)C(=NCC(=N[C@H](CCCCN)C(=O)O)O)O)O)O)O)O)O)O)O)O)O)O)O)N=C([C@H](CS)N=C([C@H](CO)N=C([C@H](CO)N=C([C@H](C)N=C(CN=C([C@H](CO)N=C([C@H](CS)N=C(CN=C(C(CS)N=C(C(CC(=O)O)N=C(CN)O)O)O)O)O)O)O)O)O)O)O)O DIGQNXIGRZPYDK-WKSCXVIASA-N 0.000 description 1
- AGNGYMCLFWQVGX-AGFFZDDWSA-N (e)-1-[(2s)-2-amino-2-carboxyethoxy]-2-diazonioethenolate Chemical compound OC(=O)[C@@H](N)CO\C([O-])=C\[N+]#N AGNGYMCLFWQVGX-AGFFZDDWSA-N 0.000 description 1
- IHPYMWDTONKSCO-UHFFFAOYSA-N 2,2'-piperazine-1,4-diylbisethanesulfonic acid Chemical compound OS(=O)(=O)CCN1CCN(CCS(O)(=O)=O)CC1 IHPYMWDTONKSCO-UHFFFAOYSA-N 0.000 description 1
- QKNYBSVHEMOAJP-UHFFFAOYSA-N 2-amino-2-(hydroxymethyl)propane-1,3-diol;hydron;chloride Chemical compound Cl.OCC(N)(CO)CO QKNYBSVHEMOAJP-UHFFFAOYSA-N 0.000 description 1
- GFFGJBXGBJISGV-FUPOQFPWSA-N 2-tritio-7h-purin-6-amine Chemical compound [3H]C1=NC(N)=C2NC=NC2=N1 GFFGJBXGBJISGV-FUPOQFPWSA-N 0.000 description 1
- 101150017816 40 gene Proteins 0.000 description 1
- 102100022464 5'-nucleotidase Human genes 0.000 description 1
- 241000271566 Aves Species 0.000 description 1
- 241000713838 Avian myeloblastosis virus Species 0.000 description 1
- 101150015280 Cel gene Proteins 0.000 description 1
- 108091026890 Coding region Proteins 0.000 description 1
- 229920000742 Cotton Polymers 0.000 description 1
- 230000026774 DNA mediated transformation Effects 0.000 description 1
- 108700003861 Dominant Genes Proteins 0.000 description 1
- 241000255581 Drosophila <fruit fly, genus> Species 0.000 description 1
- 102100031780 Endonuclease Human genes 0.000 description 1
- 108010067770 Endopeptidase K Proteins 0.000 description 1
- 241000701959 Escherichia virus Lambda Species 0.000 description 1
- 241000702315 Escherichia virus phiX174 Species 0.000 description 1
- 241000233866 Fungi Species 0.000 description 1
- 206010064571 Gene mutation Diseases 0.000 description 1
- 241000175212 Herpesvirales Species 0.000 description 1
- 102000006947 Histones Human genes 0.000 description 1
- 241000701109 Human adenovirus 2 Species 0.000 description 1
- 206010062767 Hypophysitis Diseases 0.000 description 1
- 206010061218 Inflammation Diseases 0.000 description 1
- FYYHWMGAXLPEAU-UHFFFAOYSA-N Magnesium Chemical compound [Mg] FYYHWMGAXLPEAU-UHFFFAOYSA-N 0.000 description 1
- 241000124008 Mammalia Species 0.000 description 1
- 108091027974 Mature messenger RNA Proteins 0.000 description 1
- 102000003792 Metallothionein Human genes 0.000 description 1
- 108090000157 Metallothionein Proteins 0.000 description 1
- 241000357437 Mola Species 0.000 description 1
- JJWSNOOGIUMOEE-UHFFFAOYSA-N Monomethylmercury Chemical compound [Hg]C JJWSNOOGIUMOEE-UHFFFAOYSA-N 0.000 description 1
- 241000713333 Mouse mammary tumor virus Species 0.000 description 1
- 108700005084 Multigene Family Proteins 0.000 description 1
- 101100005882 Mus musculus Cel gene Proteins 0.000 description 1
- 101000836210 Mus musculus Somatotropin Proteins 0.000 description 1
- VZUNGTLZRAYYDE-UHFFFAOYSA-N N-methyl-N'-nitro-N-nitrosoguanidine Chemical compound O=NN(C)C(=N)N[N+]([O-])=O VZUNGTLZRAYYDE-UHFFFAOYSA-N 0.000 description 1
- 101710149086 Nuclease S1 Proteins 0.000 description 1
- 102000004316 Oxidoreductases Human genes 0.000 description 1
- 229910019142 PO4 Inorganic materials 0.000 description 1
- ISWSIDIOOBJBQZ-UHFFFAOYSA-N Phenol Chemical compound OC1=CC=CC=C1 ISWSIDIOOBJBQZ-UHFFFAOYSA-N 0.000 description 1
- 239000000381 Placental Lactogen Substances 0.000 description 1
- 102000004576 Placental Lactogen Human genes 0.000 description 1
- 108010003044 Placental Lactogen Proteins 0.000 description 1
- 102100024819 Prolactin Human genes 0.000 description 1
- 108010057464 Prolactin Proteins 0.000 description 1
- 230000021839 RNA stabilization Effects 0.000 description 1
- 101001075370 Rattus norvegicus Gamma-glutamyl hydrolase Proteins 0.000 description 1
- 101000868151 Rattus norvegicus Somatotropin Proteins 0.000 description 1
- 240000004808 Saccharomyces cerevisiae Species 0.000 description 1
- 238000012300 Sequence Analysis Methods 0.000 description 1
- 108010085012 Steroid Receptors Proteins 0.000 description 1
- 108700005078 Synthetic Genes Proteins 0.000 description 1
- RZCIEJXAILMSQK-JXOAFFINSA-N TTP Chemical compound O=C1NC(=O)C(C)=CN1[C@H]1[C@H](O)[C@H](O)[C@@H](COP(O)(=O)OP(O)(=O)OP(O)(O)=O)O1 RZCIEJXAILMSQK-JXOAFFINSA-N 0.000 description 1
- 206010043276 Teratoma Diseases 0.000 description 1
- 108700009124 Transcription Initiation Site Proteins 0.000 description 1
- XSQUKJJJFZCRTK-UHFFFAOYSA-N Urea Chemical compound NC(N)=O XSQUKJJJFZCRTK-UHFFFAOYSA-N 0.000 description 1
- 241000251539 Vertebrata <Metazoa> Species 0.000 description 1
- 108020005202 Viral DNA Proteins 0.000 description 1
- 241000700605 Viruses Species 0.000 description 1
- 239000011149 active material Substances 0.000 description 1
- 230000002730 additional effect Effects 0.000 description 1
- 210000000577 adipose tissue Anatomy 0.000 description 1
- 239000003470 adrenal cortex hormone Substances 0.000 description 1
- 238000013019 agitation Methods 0.000 description 1
- 230000004075 alteration Effects 0.000 description 1
- -1 antibodies Proteins 0.000 description 1
- 230000000890 antigenic effect Effects 0.000 description 1
- 239000008346 aqueous phase Substances 0.000 description 1
- 238000003491 array Methods 0.000 description 1
- 230000003416 augmentation Effects 0.000 description 1
- 229950011321 azaserine Drugs 0.000 description 1
- 239000003150 biochemical marker Substances 0.000 description 1
- 229920001222 biopolymer Polymers 0.000 description 1
- 210000000601 blood cell Anatomy 0.000 description 1
- 238000007664 blowing Methods 0.000 description 1
- 210000000988 bone and bone Anatomy 0.000 description 1
- 210000001185 bone marrow Anatomy 0.000 description 1
- 229940098773 bovine serum albumin Drugs 0.000 description 1
- 239000007975 buffered saline Substances 0.000 description 1
- 238000010804 cDNA synthesis Methods 0.000 description 1
- 238000004364 calculation method Methods 0.000 description 1
- 239000004202 carbamide Substances 0.000 description 1
- 239000000969 carrier Substances 0.000 description 1
- 230000024245 cell differentiation Effects 0.000 description 1
- 230000032823 cell division Effects 0.000 description 1
- 239000013592 cell lysate Substances 0.000 description 1
- 210000003855 cell nucleus Anatomy 0.000 description 1
- 239000006285 cell suspension Substances 0.000 description 1
- 230000006790 cellular biosynthetic process Effects 0.000 description 1
- 230000030570 cellular localization Effects 0.000 description 1
- 230000007248 cellular mechanism Effects 0.000 description 1
- 230000019522 cellular metabolic process Effects 0.000 description 1
- 239000003610 charcoal Substances 0.000 description 1
- 239000007795 chemical reaction product Substances 0.000 description 1
- 239000013626 chemical specie Substances 0.000 description 1
- 229960005091 chloramphenicol Drugs 0.000 description 1
- WIIZWVCIJKGZOK-RKDXNWHRSA-N chloramphenicol Chemical compound ClC(Cl)C(=O)N[C@H](CO)[C@H](O)C1=CC=C([N+]([O-])=O)C=C1 WIIZWVCIJKGZOK-RKDXNWHRSA-N 0.000 description 1
- 238000000975 co-precipitation Methods 0.000 description 1
- 238000010276 construction Methods 0.000 description 1
- 239000000356 contaminant Substances 0.000 description 1
- 238000011109 contamination Methods 0.000 description 1
- 229960001334 corticosteroids Drugs 0.000 description 1
- RKHQGWMMUURILY-UHRZLXHJSA-N cortivazol Chemical compound C([C@H]1[C@@H]2C[C@H]([C@]([C@@]2(C)C[C@H](O)[C@@H]1[C@@]1(C)C2)(O)C(=O)COC(C)=O)C)=C(C)C1=CC1=C2C=NN1C1=CC=CC=C1 RKHQGWMMUURILY-UHRZLXHJSA-N 0.000 description 1
- GYOZYWVXFNDGLU-XLPZGREQSA-N dTMP Chemical compound O=C1NC(=O)C(C)=CN1[C@@H]1O[C@H](COP(O)(O)=O)[C@@H](O)C1 GYOZYWVXFNDGLU-XLPZGREQSA-N 0.000 description 1
- 230000006378 damage Effects 0.000 description 1
- 230000034994 death Effects 0.000 description 1
- 230000007547 defect Effects 0.000 description 1
- 238000013461 design Methods 0.000 description 1
- 239000000032 diagnostic agent Substances 0.000 description 1
- OZRNSSUDZOLUSN-LBPRGKRZSA-N dihydrofolic acid Chemical compound N=1C=2C(=O)NC(N)=NC=2NCC=1CNC1=CC=C(C(=O)N[C@@H](CCC(O)=O)C(O)=O)C=C1 OZRNSSUDZOLUSN-LBPRGKRZSA-N 0.000 description 1
- 230000003292 diminished effect Effects 0.000 description 1
- LOKCTEFSRHRXRJ-UHFFFAOYSA-I dipotassium trisodium dihydrogen phosphate hydrogen phosphate dichloride Chemical compound P(=O)(O)(O)[O-].[K+].P(=O)(O)([O-])[O-].[Na+].[Na+].[Cl-].[K+].[Cl-].[Na+] LOKCTEFSRHRXRJ-UHFFFAOYSA-I 0.000 description 1
- 201000010099 disease Diseases 0.000 description 1
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 1
- 210000002257 embryonic structure Anatomy 0.000 description 1
- 210000000981 epithelium Anatomy 0.000 description 1
- 239000003797 essential amino acid Substances 0.000 description 1
- 235000020776 essential amino acid Nutrition 0.000 description 1
- ZMMJGEGLRURXTF-UHFFFAOYSA-N ethidium bromide Chemical compound [Br-].C12=CC(N)=CC=C2C2=CC=C(N)C=C2[N+](CC)=C1C1=CC=CC=C1 ZMMJGEGLRURXTF-UHFFFAOYSA-N 0.000 description 1
- 229960005542 ethidium bromide Drugs 0.000 description 1
- DNJIEGIFACGWOD-UHFFFAOYSA-N ethyl mercaptane Natural products CCS DNJIEGIFACGWOD-UHFFFAOYSA-N 0.000 description 1
- 210000004265 eukaryotic small ribosome subunit Anatomy 0.000 description 1
- 238000013401 experimental design Methods 0.000 description 1
- 239000011536 extraction buffer Substances 0.000 description 1
- 239000012894 fetal calf serum Substances 0.000 description 1
- 238000001914 filtration Methods 0.000 description 1
- 229940014144 folate Drugs 0.000 description 1
- OVBPIULPVIDEAO-LBPRGKRZSA-N folic acid Chemical compound C=1N=C2NC(N)=NC(=O)C2=NC=1CNC1=CC=C(C(=O)N[C@@H](CCC(O)=O)C(O)=O)C=C1 OVBPIULPVIDEAO-LBPRGKRZSA-N 0.000 description 1
- 235000019152 folic acid Nutrition 0.000 description 1
- 239000011724 folic acid Substances 0.000 description 1
- 101150055782 gH gene Proteins 0.000 description 1
- 238000010353 genetic engineering Methods 0.000 description 1
- 210000004602 germ cell Anatomy 0.000 description 1
- 230000012010 growth Effects 0.000 description 1
- 229960000789 guanidine hydrochloride Drugs 0.000 description 1
- PJJJBBJSCAKJQF-UHFFFAOYSA-N guanidinium chloride Chemical compound [Cl-].NC(N)=[NH2+] PJJJBBJSCAKJQF-UHFFFAOYSA-N 0.000 description 1
- ZJYYHGLJYGJLLN-UHFFFAOYSA-N guanidinium thiocyanate Chemical compound SC#N.NC(N)=N ZJYYHGLJYGJLLN-UHFFFAOYSA-N 0.000 description 1
- 125000005842 heteroatom Chemical group 0.000 description 1
- 210000005260 human cell Anatomy 0.000 description 1
- 230000001900 immune effect Effects 0.000 description 1
- 238000010324 immunological assay Methods 0.000 description 1
- 230000004054 inflammatory process Effects 0.000 description 1
- 239000003112 inhibitor Substances 0.000 description 1
- 230000005764 inhibitory process Effects 0.000 description 1
- 239000003999 initiator Substances 0.000 description 1
- 238000002347 injection Methods 0.000 description 1
- 239000007924 injection Substances 0.000 description 1
- 230000002452 interceptive effect Effects 0.000 description 1
- 238000001155 isoelectric focusing Methods 0.000 description 1
- 238000001738 isopycnic centrifugation Methods 0.000 description 1
- 231100000518 lethal Toxicity 0.000 description 1
- 230000001665 lethal effect Effects 0.000 description 1
- 210000000265 leukocyte Anatomy 0.000 description 1
- 238000005567 liquid scintillation counting Methods 0.000 description 1
- 210000005229 liver cell Anatomy 0.000 description 1
- 238000011068 loading method Methods 0.000 description 1
- 239000006166 lysate Substances 0.000 description 1
- 239000011777 magnesium Substances 0.000 description 1
- 229910052749 magnesium Inorganic materials 0.000 description 1
- 230000003211 malignant effect Effects 0.000 description 1
- 230000007102 metabolic function Effects 0.000 description 1
- KRZWEBVPFGCYMY-UHFFFAOYSA-M methylmercury(1+);hydroxide Chemical compound [OH-].[Hg+]C KRZWEBVPFGCYMY-UHFFFAOYSA-M 0.000 description 1
- 230000002438 mitochondrial effect Effects 0.000 description 1
- 230000009456 molecular mechanism Effects 0.000 description 1
- 238000004264 monolayer culture Methods 0.000 description 1
- 230000000877 morphologic effect Effects 0.000 description 1
- 210000003205 muscle Anatomy 0.000 description 1
- 239000003471 mutagenic agent Substances 0.000 description 1
- 231100000707 mutagenic chemical Toxicity 0.000 description 1
- 230000003505 mutagenic effect Effects 0.000 description 1
- 239000013642 negative control Substances 0.000 description 1
- 210000000441 neoplastic stem cell Anatomy 0.000 description 1
- 230000001537 neural effect Effects 0.000 description 1
- 210000000633 nuclear envelope Anatomy 0.000 description 1
- 102000039446 nucleic acids Human genes 0.000 description 1
- 108020004707 nucleic acids Proteins 0.000 description 1
- 150000007523 nucleic acids Chemical class 0.000 description 1
- 108010028584 nucleotidase Proteins 0.000 description 1
- 235000015097 nutrients Nutrition 0.000 description 1
- 210000001672 ovary Anatomy 0.000 description 1
- 230000002688 persistence Effects 0.000 description 1
- 150000002989 phenols Chemical class 0.000 description 1
- 208000016021 phenotype Diseases 0.000 description 1
- NBIIXXVUZAFLBC-UHFFFAOYSA-K phosphate Chemical compound [O-]P([O-])([O-])=O NBIIXXVUZAFLBC-UHFFFAOYSA-K 0.000 description 1
- 239000010452 phosphate Substances 0.000 description 1
- 210000003635 pituitary gland Anatomy 0.000 description 1
- 230000008488 polyadenylation Effects 0.000 description 1
- 229920001184 polypeptide Polymers 0.000 description 1
- 102000004196 processed proteins & peptides Human genes 0.000 description 1
- 108090000765 processed proteins & peptides Proteins 0.000 description 1
- 229940097325 prolactin Drugs 0.000 description 1
- 230000000644 propagated effect Effects 0.000 description 1
- 108010043671 prostatic acid phosphatase Proteins 0.000 description 1
- 238000001243 protein synthesis Methods 0.000 description 1
- 238000004080 punching Methods 0.000 description 1
- 230000010076 replication Effects 0.000 description 1
- 108020004418 ribosomal RNA Proteins 0.000 description 1
- 210000003705 ribosome Anatomy 0.000 description 1
- 229920002477 rna polymer Polymers 0.000 description 1
- 238000007790 scraping Methods 0.000 description 1
- 230000028327 secretion Effects 0.000 description 1
- 230000000405 serological effect Effects 0.000 description 1
- 210000003765 sex chromosome Anatomy 0.000 description 1
- 230000035939 shock Effects 0.000 description 1
- 239000001509 sodium citrate Substances 0.000 description 1
- NLJMYIDDQXHKNR-UHFFFAOYSA-K sodium citrate Chemical compound O.O.[Na+].[Na+].[Na+].[O-]C(=O)CC(O)(CC([O-])=O)C([O-])=O NLJMYIDDQXHKNR-UHFFFAOYSA-K 0.000 description 1
- AJPJDKMHJJGVTQ-UHFFFAOYSA-M sodium dihydrogen phosphate Chemical compound [Na+].OP(O)([O-])=O AJPJDKMHJJGVTQ-UHFFFAOYSA-M 0.000 description 1
- FQENQNTWSFEDLI-UHFFFAOYSA-J sodium diphosphate Chemical compound [Na+].[Na+].[Na+].[Na+].[O-]P([O-])(=O)OP([O-])([O-])=O FQENQNTWSFEDLI-UHFFFAOYSA-J 0.000 description 1
- 229940048086 sodium pyrophosphate Drugs 0.000 description 1
- 239000007787 solid Substances 0.000 description 1
- 230000000392 somatic effect Effects 0.000 description 1
- 210000002325 somatostatin-secreting cell Anatomy 0.000 description 1
- 238000000527 sonication Methods 0.000 description 1
- 230000006641 stabilisation Effects 0.000 description 1
- 238000011105 stabilization Methods 0.000 description 1
- 238000010186 staining Methods 0.000 description 1
- 108020003113 steroid hormone receptors Proteins 0.000 description 1
- 150000003431 steroids Chemical class 0.000 description 1
- 150000008163 sugars Chemical class 0.000 description 1
- 239000012134 supernatant fraction Substances 0.000 description 1
- 208000024891 symptom Diseases 0.000 description 1
- 210000001550 testis Anatomy 0.000 description 1
- 235000019818 tetrasodium diphosphate Nutrition 0.000 description 1
- 239000001577 tetrasodium phosphonato phosphate Substances 0.000 description 1
- 238000002560 therapeutic procedure Methods 0.000 description 1
- 239000003104 tissue culture media Substances 0.000 description 1
- 230000005026 transcription initiation Effects 0.000 description 1
- 239000001226 triphosphate Substances 0.000 description 1
- 235000011178 triphosphate Nutrition 0.000 description 1
- 125000002264 triphosphate group Chemical class [H]OP(=O)(O[H])OP(=O)(O[H])OP(=O)(O[H])O* 0.000 description 1
- 210000005239 tubule Anatomy 0.000 description 1
- 101150101459 tx gene Proteins 0.000 description 1
- 241001529453 unidentified herpesvirus Species 0.000 description 1
- 238000011144 upstream manufacturing Methods 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
Landscapes
- Genetics & Genomics (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Wood Science & Technology (AREA)
- Organic Chemistry (AREA)
- Biotechnology (AREA)
- General Engineering & Computer Science (AREA)
- Biomedical Technology (AREA)
- Zoology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Molecular Biology (AREA)
- Microbiology (AREA)
- Plant Pathology (AREA)
- Physics & Mathematics (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- Biophysics (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Peptides Or Proteins (AREA)
Applications Claiming Priority (3)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US40678582A | 1982-08-10 | 1982-08-10 | |
US406785 | 1982-08-10 | ||
PCT/US1983/001240 WO1984000775A1 (en) | 1982-08-10 | 1983-08-10 | The use of eucaryotic promoter sequences in the production of proteinaceous materials |
Publications (1)
Publication Number | Publication Date |
---|---|
AU2038283A true AU2038283A (en) | 1984-03-07 |
Family
ID=23609447
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
AU20382/83A Abandoned AU2038283A (en) | 1982-08-10 | 1983-08-10 | The use of eucaryotic promoter sequences in the production ofproteinaceous materials |
Country Status (4)
Country | Link |
---|---|
EP (1) | EP0116631A4 (de) |
JP (1) | JPS59501533A (de) |
AU (1) | AU2038283A (de) |
WO (1) | WO1984000775A1 (de) |
Families Citing this family (6)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US4840896A (en) * | 1983-11-02 | 1989-06-20 | Integrated Genetics, Inc. | Heteropolymeric protein |
FR2601964A1 (fr) * | 1986-07-23 | 1988-01-29 | Pasteur Institut | Procede de production in vitro d'un polypeptide determine dans des cellules de mammiferes ou des hybrides de celles-ci, et comportant integree dans leur genome la sequence codante pour le polypeptide determine. |
US5614395A (en) * | 1988-03-08 | 1997-03-25 | Ciba-Geigy Corporation | Chemically regulatable and anti-pathogenic DNA sequences and uses thereof |
US6599510B1 (en) * | 1993-11-23 | 2003-07-29 | The Regents Of The University Of California | Abundant extracellular products and methods for their production and use |
US6475725B1 (en) | 1997-06-20 | 2002-11-05 | Baxter Aktiengesellschaft | Recombinant cell clones having increased stability and methods of making and using the same |
WO2022029300A1 (de) | 2020-08-06 | 2022-02-10 | Sms Group Gmbh | VAKUUM-INDUKTIONS-GIEßEINRICHTUNG ZUM GIEßEN VON METALL UND METALLLEGIERUNGEN UNTER VAKUUM UND/ODER SCHUTZGASATMOSPHÄRE SOWIE VERFAHREN ZUM WECHSELN EINER STOPFENSTANGE UND/ODER EINES VERSCHLUSSKÖRPERS EINER STOPFENGIEßVORRICHTUNG AN EINER VAKUUM-INDUKTIONS-GIEßEINRICHTUNG |
Family Cites Families (5)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US4085203A (en) * | 1972-04-26 | 1978-04-18 | Burroughs Wellcome Co. | Process for preparing vaccine |
GB2016015B (en) * | 1978-01-22 | 1982-05-06 | Hayashibara Co | Method of preparing interferon and preparations containing interferon |
US4342832A (en) * | 1979-07-05 | 1982-08-03 | Genentech, Inc. | Method of constructing a replicable cloning vehicle having quasi-synthetic genes |
WO1981002425A1 (en) * | 1980-02-25 | 1981-09-03 | R Axel | The use of eucaryotic promoter sequences in the production of proteinaceous materials |
US4396601A (en) * | 1980-03-26 | 1983-08-02 | The Regents Of The University Of Calif. | Gene transfer in intact mammals |
-
1983
- 1983-08-10 AU AU20382/83A patent/AU2038283A/en not_active Abandoned
- 1983-08-10 WO PCT/US1983/001240 patent/WO1984000775A1/en not_active Application Discontinuation
- 1983-08-10 JP JP83502984A patent/JPS59501533A/ja active Pending
- 1983-08-10 EP EP19830902926 patent/EP0116631A4/de not_active Withdrawn
Also Published As
Publication number | Publication date |
---|---|
WO1984000775A1 (en) | 1984-03-01 |
EP0116631A1 (de) | 1984-08-29 |
EP0116631A4 (de) | 1986-04-15 |
JPS59501533A (ja) | 1984-08-30 |
Similar Documents
Publication | Publication Date | Title |
---|---|---|
AU558061B2 (en) | Processes for inserting dna into eucaryotic cells and for producing proteinaceous materials | |
US4634665A (en) | Processes for inserting DNA into eucaryotic cells and for producing proteinaceous materials | |
US5179017A (en) | Processes for inserting DNA into eucaryotic cells and for producing proteinaceous materials | |
US4675285A (en) | Method for identification and isolation of DNA encoding a desired protein | |
Crouse et al. | Expression and amplification of engineered mouse dihydrofolate reductase minigenes | |
WO1981002425A1 (en) | The use of eucaryotic promoter sequences in the production of proteinaceous materials | |
Mayo et al. | Glucocorticoid regulation of the mouse metallothionein I gene is selectively lost following amplification of the gene. | |
JPH07106156B2 (ja) | ファクタ−▲viii▼および関連生産物の製造 | |
JPH09103296A (ja) | 安定化配列を有するベクターおよび真核宿主細胞 | |
US6455275B1 (en) | DNA construct for producing proteinaceous materials in eucaryotic cells | |
US4761375A (en) | Human interleukin-2 cDNA sequence | |
AU671801B2 (en) | Gene manipulation and expression using genomic elements | |
AU2038283A (en) | The use of eucaryotic promoter sequences in the production ofproteinaceous materials | |
EP0103632A1 (de) | Verfahren zum einbringen von klonierten amplifizierbaren genen in eukaryotische zellen und zur herstellung von proteinprodukten. | |
US5238820A (en) | Multiply-amplifiable vectors for high level expression of exogenuos DNA | |
Miesfeld et al. | The absence of a human-specific ribosomal DNA transcription factor leads to nucleolar dominance in mouse> human hybrid cells | |
Perucho et al. | Linkage and expression of foreign DNA in cultured animal cells | |
Looney et al. | The dihydrofolate reductase amplicons in different methotrexate-resistant Chinese hamster cell lines share at least a 273-kilobase core sequence, but the amplicons in some cell lines are much larger and are remarkably uniform in structure | |
JPS6143121A (ja) | 組換え体因子8―r | |
WO1987002707A1 (en) | Multiply-amplifiable vectors for high level expression of exogenous dna | |
EP0546333A1 (de) | DNS Klone mit 'Enhancer' Aktivität, rekombinanter Vektor der es enthält und Verfahren zur Herstellung von Genprodukten unter Verwendung desselben | |
US5151511A (en) | DNA encoding avian growth hormones | |
CA1218949A (en) | Use of eucaryotic promoter sequences in the production of proteinaceous materials | |
AU583018B2 (en) | Gibbon interleukin | |
EP0106170A2 (de) | Vogelwachstumshormone |