AU2022413687A1 - Methods for enrichment of circular rna under denaturing conditions - Google Patents

Methods for enrichment of circular rna under denaturing conditions Download PDF

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Publication number
AU2022413687A1
AU2022413687A1 AU2022413687A AU2022413687A AU2022413687A1 AU 2022413687 A1 AU2022413687 A1 AU 2022413687A1 AU 2022413687 A AU2022413687 A AU 2022413687A AU 2022413687 A AU2022413687 A AU 2022413687A AU 2022413687 A1 AU2022413687 A1 AU 2022413687A1
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circrna
acid
nucleotides
linrna
population
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Anthony Joseph CURA
Alexandra Sophie DE BOER
Joshua Nathan FARB
Dineshkumar MANVAR
Tushar Kanti MISRA
Jennifer A. Nelson
Nicholas McCartney PLUGIS
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Flagship Pioneering Innovations VI Inc
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    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/10Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1003Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
    • C12N15/1006Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by means of a solid support carrier, e.g. particles, polymers
    • C12N15/101Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by means of a solid support carrier, e.g. particles, polymers by chromatography, e.g. electrophoresis, ion-exchange, reverse phase
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/11DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/11DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/113Non-coding nucleic acids modulating the expression of genes, e.g. antisense oligonucleotides; Antisense DNA or RNA; Triplex- forming oligonucleotides; Catalytic nucleic acids, e.g. ribozymes; Nucleic acids used in co-suppression or gene silencing
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2310/00Structure or type of the nucleic acid
    • C12N2310/50Physical structure
    • C12N2310/53Physical structure partially self-complementary or closed
    • C12N2310/532Closed or circular

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  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Molecular Biology (AREA)
  • Analytical Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Plant Pathology (AREA)
  • Biophysics (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Saccharide Compounds (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
AU2022413687A 2021-12-17 2022-12-16 Methods for enrichment of circular rna under denaturing conditions Pending AU2022413687A1 (en)

Applications Claiming Priority (3)

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US202163291185P 2021-12-17 2021-12-17
US63/291,185 2021-12-17
PCT/US2022/081826 WO2023115013A1 (en) 2021-12-17 2022-12-16 Methods for enrichment of circular rna under denaturing conditions

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AU2022413687A1 true AU2022413687A1 (en) 2024-06-27

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US (1) US20240401024A1 (https=)
EP (1) EP4448758A1 (https=)
JP (1) JP2024546855A (https=)
KR (1) KR20240118174A (https=)
CN (1) CN118510896A (https=)
AR (1) AR128002A1 (https=)
AU (1) AU2022413687A1 (https=)
CA (1) CA3240691A1 (https=)
MX (1) MX2024007307A (https=)
TW (1) TW202340460A (https=)
WO (1) WO2023115013A1 (https=)

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US20230203192A1 (en) * 2020-05-20 2023-06-29 Flagship Pioneering, Inc. Compositions and methods for producing human polyclonal antibodies

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KR20240118174A (ko) 2024-08-02
EP4448758A1 (en) 2024-10-23
WO2023115013A1 (en) 2023-06-22
AR128002A1 (es) 2024-03-20
CN118510896A (zh) 2024-08-16
MX2024007307A (es) 2024-08-06
US20240401024A1 (en) 2024-12-05
CA3240691A1 (en) 2023-06-22
TW202340460A (zh) 2023-10-16
JP2024546855A (ja) 2024-12-26

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