AU2022405520A1 - Omni-103 crispr nuclease-rna complexes - Google Patents

Omni-103 crispr nuclease-rna complexes Download PDF

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Publication number
AU2022405520A1
AU2022405520A1 AU2022405520A AU2022405520A AU2022405520A1 AU 2022405520 A1 AU2022405520 A1 AU 2022405520A1 AU 2022405520 A AU2022405520 A AU 2022405520A AU 2022405520 A AU2022405520 A AU 2022405520A AU 2022405520 A1 AU2022405520 A1 AU 2022405520A1
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Australia
Prior art keywords
sequence
composition
tracrrna
seq
nucleotides
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Pending
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AU2022405520A
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English (en)
Inventor
Nir HECHT
Lior IZHAR
Nadav MARBACH BAR
Liat ROCKAH
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Emendobio Inc
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Emendobio Inc
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Publication of AU2022405520A1 publication Critical patent/AU2022405520A1/en
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    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/113—Non-coding nucleic acids modulating the expression of genes, e.g. antisense oligonucleotides; Antisense DNA or RNA; Triplex- forming oligonucleotides; Catalytic nucleic acids, e.g. ribozymes; Nucleic acids used in co-suppression or gene silencing
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    • C12N15/09—Recombinant DNA-technology
    • C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
    • C12N15/90—Stable introduction of foreign DNA into chromosome
    • C12N15/902—Stable introduction of foreign DNA into chromosome using homologous recombination
    • C12N15/907—Stable introduction of foreign DNA into chromosome using homologous recombination in mammalian cells
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    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/102—Mutagenizing nucleic acids
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    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/113—Non-coding nucleic acids modulating the expression of genes, e.g. antisense oligonucleotides; Antisense DNA or RNA; Triplex- forming oligonucleotides; Catalytic nucleic acids, e.g. ribozymes; Nucleic acids used in co-suppression or gene silencing
    • C12N15/1138—Non-coding nucleic acids modulating the expression of genes, e.g. antisense oligonucleotides; Antisense DNA or RNA; Triplex- forming oligonucleotides; Catalytic nucleic acids, e.g. ribozymes; Nucleic acids used in co-suppression or gene silencing against receptors or cell surface proteins
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
    • C12N9/22—Ribonucleases [RNase]; Deoxyribonucleases [DNase]
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    • C12N2310/00—Structure or type of the nucleic acid
    • C12N2310/10—Type of nucleic acid
    • C12N2310/20—Type of nucleic acid involving clustered regularly interspaced short palindromic repeats [CRISPR]
    • C—CHEMISTRY; METALLURGY
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    • C12N2310/00—Structure or type of the nucleic acid
    • C12N2310/30—Chemical structure
    • C12N2310/31—Chemical structure of the backbone
    • C12N2310/315—Phosphorothioates
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    • C12N2310/00—Structure or type of the nucleic acid
    • C12N2310/30—Chemical structure
    • C12N2310/32—Chemical structure of the sugar
    • C12N2310/321—2'-O-R Modification
    • C—CHEMISTRY; METALLURGY
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    • C12N2310/00—Structure or type of the nucleic acid
    • C12N2310/50—Physical structure
    • C12N2310/53—Physical structure partially self-complementary or closed
    • C12N2310/531—Stem-loop; Hairpin

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  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
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  • Biotechnology (AREA)
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  • General Health & Medical Sciences (AREA)
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  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
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  • Crystallography & Structural Chemistry (AREA)
  • Cell Biology (AREA)
  • Mycology (AREA)
  • Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Enzymes And Modification Thereof (AREA)
AU2022405520A 2021-12-07 2022-12-06 Omni-103 crispr nuclease-rna complexes Pending AU2022405520A1 (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US202163286855P 2021-12-07 2021-12-07
US63/286,855 2021-12-07
PCT/US2022/081019 WO2023107946A2 (en) 2021-12-07 2022-12-06 Omni-103 crispr nuclease-rna complexes

Publications (1)

Publication Number Publication Date
AU2022405520A1 true AU2022405520A1 (en) 2024-07-04

Family

ID=86731282

Family Applications (1)

Application Number Title Priority Date Filing Date
AU2022405520A Pending AU2022405520A1 (en) 2021-12-07 2022-12-06 Omni-103 crispr nuclease-rna complexes

Country Status (9)

Country Link
US (1) US20250034596A1 (https=)
EP (1) EP4444876A2 (https=)
JP (1) JP2024545144A (https=)
KR (1) KR20240135610A (https=)
CN (2) CN116887854A (https=)
AU (1) AU2022405520A1 (https=)
CA (1) CA3240329A1 (https=)
IL (1) IL313397A (https=)
WO (1) WO2023107946A2 (https=)

Families Citing this family (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2024506608A (ja) 2021-02-08 2024-02-14 エメンドバイオ・インコーポレイテッド Ｏｍｎｉ－１０３ ｃｒｉｓｐｒヌクレアーゼ
US12091688B2 (en) 2021-02-08 2024-09-17 Emendobio Inc. OMNI-103 CRISPR nuclease
CN120837655A (zh) * 2025-09-24 2025-10-28 中国人民解放军军事科学院军事医学研究院 磷酸化抑制工具在制备预防和/或治疗三阴性乳腺癌的药物中的用途

Family Cites Families (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2020069029A1 (en) * 2018-09-26 2020-04-02 Emendobio Inc. Novel crispr nucleases
IL298577A (en) * 2020-05-27 2023-01-01 Emendobio Inc Biallelic silencing of sarm1

Also Published As

Publication number Publication date
KR20240135610A (ko) 2024-09-11
WO2023107946A3 (en) 2023-08-03
IL313397A (en) 2024-08-01
CN118660967A (zh) 2024-09-17
EP4444876A2 (en) 2024-10-16
US20250034596A1 (en) 2025-01-30
CN116887854A (zh) 2023-10-13
WO2023107946A2 (en) 2023-06-15
CA3240329A1 (en) 2023-06-15
JP2024545144A (ja) 2024-12-05

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