AU2022283895A1 - Gene editing in primary immune cells using cell penetrating crispr-cas system - Google Patents

Gene editing in primary immune cells using cell penetrating crispr-cas system Download PDF

Info

Publication number
AU2022283895A1
AU2022283895A1 AU2022283895A AU2022283895A AU2022283895A1 AU 2022283895 A1 AU2022283895 A1 AU 2022283895A1 AU 2022283895 A AU2022283895 A AU 2022283895A AU 2022283895 A AU2022283895 A AU 2022283895A AU 2022283895 A1 AU2022283895 A1 AU 2022283895A1
Authority
AU
Australia
Prior art keywords
cells
cell
cas9
cas
cpp
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
AU2022283895A
Other languages
English (en)
Other versions
AU2022283895A9 (en
Inventor
Amy Elizabeth Baxter
Shelley L. Berger
Zeyu Chen
Rahul M. KOHLI
Jared B. PARKER
Junwei Shi
E. John Wherry
Zhen Zhang
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
University of Pennsylvania Penn
Original Assignee
University of Pennsylvania Penn
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by University of Pennsylvania Penn filed Critical University of Pennsylvania Penn
Publication of AU2022283895A1 publication Critical patent/AU2022283895A1/en
Publication of AU2022283895A9 publication Critical patent/AU2022283895A9/en
Pending legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
    • C12N9/22—Ribonucleases [RNase]; Deoxyribonucleases [DNase]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/102—Mutagenizing nucleic acids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/111—General methods applicable to biologically active non-coding nucleic acids
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/01—Fusion polypeptide containing a localisation/targetting motif
    • C07K2319/06—Fusion polypeptide containing a localisation/targetting motif containing a lysosomal/endosomal localisation signal
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/01—Fusion polypeptide containing a localisation/targetting motif
    • C07K2319/09—Fusion polypeptide containing a localisation/targetting motif containing a nuclear localisation signal
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/01—Fusion polypeptide containing a localisation/targetting motif
    • C07K2319/10—Fusion polypeptide containing a localisation/targetting motif containing a tag for extracellular membrane crossing, e.g. TAT or VP22
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2310/00—Structure or type of the nucleic acid
    • C12N2310/10—Type of nucleic acid
    • C12N2310/20—Type of nucleic acid involving clustered regularly interspaced short palindromic repeats [CRISPR]

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Chemical & Material Sciences (AREA)
  • Biomedical Technology (AREA)
  • Organic Chemistry (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Plant Pathology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Medicinal Chemistry (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Peptides Or Proteins (AREA)
  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)
AU2022283895A 2021-06-02 2022-06-02 Gene editing in primary immune cells using cell penetrating crispr-cas system Pending AU2022283895A1 (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US202163196144P 2021-06-02 2021-06-02
US63/196,144 2021-06-02
PCT/US2022/031983 WO2022256546A2 (en) 2021-06-02 2022-06-02 Gene editing in primary immune cells using cell penetrating crispr-cas system

Publications (2)

Publication Number Publication Date
AU2022283895A1 true AU2022283895A1 (en) 2023-12-14
AU2022283895A9 AU2022283895A9 (en) 2024-01-25

Family

ID=84324570

Family Applications (1)

Application Number Title Priority Date Filing Date
AU2022283895A Pending AU2022283895A1 (en) 2021-06-02 2022-06-02 Gene editing in primary immune cells using cell penetrating crispr-cas system

Country Status (7)

Country Link
US (1) US20240263159A1 (https=)
EP (1) EP4347811A4 (https=)
JP (1) JP2024520644A (https=)
CN (1) CN117795065A (https=)
AU (1) AU2022283895A1 (https=)
CA (1) CA3221125A1 (https=)
WO (1) WO2022256546A2 (https=)

Families Citing this family (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN117285599B (zh) * 2023-10-11 2024-04-09 东北农业大学 一种抗胞内细菌的细胞穿透型抗菌肽5vt及其制备方法和应用
WO2025208177A1 (en) * 2024-04-03 2025-10-09 Macquarie University Therapeutic peptides

Family Cites Families (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US10258697B2 (en) * 2014-10-29 2019-04-16 Massachusetts Eye And Ear Infirmary Efficient delivery of therapeutic molecules in vitro and in vivo
WO2017175072A1 (en) * 2016-04-08 2017-10-12 Feldan Bio Inc. Peptide shuttle based gene disruption
US12454685B2 (en) * 2019-07-08 2025-10-28 The Regents Of The University Of California Variant type V CRISPR/Cas effector polypeptides and methods of use thereof

Also Published As

Publication number Publication date
CA3221125A1 (en) 2022-12-08
JP2024520644A (ja) 2024-05-24
WO2022256546A3 (en) 2023-01-12
CN117795065A (zh) 2024-03-29
EP4347811A4 (en) 2025-06-11
WO2022256546A2 (en) 2022-12-08
EP4347811A2 (en) 2024-04-10
AU2022283895A9 (en) 2024-01-25
US20240263159A1 (en) 2024-08-08

Similar Documents

Publication Publication Date Title
JP7762072B2 (ja) 抗原特異的人工免疫制御性Ｔ（ａｉｒＴ）細胞
JP7397791B2 (ja) 初代細胞の遺伝子編集
US20230066806A1 (en) Pooled knock-in screening and heterologous polypeptides co-expressed under the control of endogenous loci
JP2021534783A (ja) キメラ抗原受容体発現細胞を作製する方法
US20210207174A1 (en) Genetic engineering of endogenous proteins
AU2020215725A1 (en) Gene-regulating compositions and methods for improved immunotherapy
US20240263159A1 (en) Gene editing in primary immune cells using cell penetrating crispr-cas system
WO2022143783A1 (en) Methods of identifying t-cell modulating genes
Cappabianca et al. Non-viral expression of chimeric antigen receptors with multiplex gene editing in primary T cells
JP2023526278A (ja) 組み換え受容体を発現しているドナーバッチ細胞を産生するための方法
US20210214722A1 (en) Methods and materials for cloning functional t cell receptors from single t cells
HK40111554A (zh) 使用细胞穿透crispr-cas系统在原代免疫细胞中进行基因编辑
Teppert Combination of CAR and TCR technologies to increase efficacy of adoptive cell therapy
HK40062498A (en) Methods for cloning functional t cell receptors from single t cells
US20230250415A1 (en) Method for introducing antigen-specific receptor gene into t cell genome using circular dna
WO2025117773A2 (en) Compositions and methods for treating neoplasia
WO2023147069A2 (en) Base editing and crispr/cas9 gene editing strategies to correct cd3 severe combined immunodeficiency in hematopoietic stem cells
Sailer Generation of universal recipient cells for the testing and characterization of transgenic TCRs
HK40072586A (en) Methods of identifying t-cell modulating genes
CN119968466A (zh) 修饰的免疫细胞及其用途
CN117165529A (zh) 一种经过基因修饰的t细胞及其制备方法和应用
HK40016014A (en) Methods and materials for cloning functional t cell receptors from single t cells
HK40016014B (en) Methods and materials for cloning functional t cell receptors from single t cells

Legal Events

Date Code Title Description
SREP Specification republished