JP2024520644A - 細胞膜透過性ｃｒｉｓｐｒ－ｃａｓシステムを用いた初代免疫細胞における遺伝子編集 - Google Patents

細胞膜透過性ｃｒｉｓｐｒ－ｃａｓシステムを用いた初代免疫細胞における遺伝子編集 Download PDF

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JP2024520644A
JP2024520644A JP2023574376A JP2023574376A JP2024520644A JP 2024520644 A JP2024520644 A JP 2024520644A JP 2023574376 A JP2023574376 A JP 2023574376A JP 2023574376 A JP2023574376 A JP 2023574376A JP 2024520644 A JP2024520644 A JP 2024520644A
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cells
cell
cas9
cas
cpp
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シェリー エル． バーガー
イー． ジョン ウェリー
ジュンウェイ シー
ゼユ チェン
ジェン ジャン
ラフル エム． コーリ
ジャレッド ビー． パーカー
エイミー エリザベス バクスター
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ザ トラスティーズ オブ ザ ユニバーシティ オブ ペンシルバニア
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    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
    • C12N9/22—Ribonucleases [RNase]; Deoxyribonucleases [DNase]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
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    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/111—General methods applicable to biologically active non-coding nucleic acids
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/01—Fusion polypeptide containing a localisation/targetting motif
    • C07K2319/06—Fusion polypeptide containing a localisation/targetting motif containing a lysosomal/endosomal localisation signal
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/01—Fusion polypeptide containing a localisation/targetting motif
    • C07K2319/09—Fusion polypeptide containing a localisation/targetting motif containing a nuclear localisation signal
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/01—Fusion polypeptide containing a localisation/targetting motif
    • C07K2319/10—Fusion polypeptide containing a localisation/targetting motif containing a tag for extracellular membrane crossing, e.g. TAT or VP22
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2310/00—Structure or type of the nucleic acid
    • C12N2310/10—Type of nucleic acid
    • C12N2310/20—Type of nucleic acid involving clustered regularly interspaced short palindromic repeats [CRISPR]

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  • Crystallography & Structural Chemistry (AREA)
  • Medicinal Chemistry (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Peptides Or Proteins (AREA)
  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)
JP2023574376A 2021-06-02 2022-06-02 細胞膜透過性ｃｒｉｓｐｒ－ｃａｓシステムを用いた初代免疫細胞における遺伝子編集 Pending JP2024520644A (ja)

Applications Claiming Priority (3)

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US202163196144P 2021-06-02 2021-06-02
US63/196,144 2021-06-02
PCT/US2022/031983 WO2022256546A2 (en) 2021-06-02 2022-06-02 Gene editing in primary immune cells using cell penetrating crispr-cas system

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JP2024520644A true JP2024520644A (ja) 2024-05-24
JP2024520644A5 JP2024520644A5 (https=) 2025-06-10

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US (1) US20240263159A1 (https=)
EP (1) EP4347811A4 (https=)
JP (1) JP2024520644A (https=)
CN (1) CN117795065A (https=)
AU (1) AU2022283895A1 (https=)
CA (1) CA3221125A1 (https=)
WO (1) WO2022256546A2 (https=)

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CN117285599B (zh) * 2023-10-11 2024-04-09 东北农业大学 一种抗胞内细菌的细胞穿透型抗菌肽5vt及其制备方法和应用
WO2025208177A1 (en) * 2024-04-03 2025-10-09 Macquarie University Therapeutic peptides

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US10258697B2 (en) * 2014-10-29 2019-04-16 Massachusetts Eye And Ear Infirmary Efficient delivery of therapeutic molecules in vitro and in vivo
WO2017175072A1 (en) * 2016-04-08 2017-10-12 Feldan Bio Inc. Peptide shuttle based gene disruption
US12454685B2 (en) * 2019-07-08 2025-10-28 The Regents Of The University Of California Variant type V CRISPR/Cas effector polypeptides and methods of use thereof

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CA3221125A1 (en) 2022-12-08
WO2022256546A3 (en) 2023-01-12
CN117795065A (zh) 2024-03-29
EP4347811A4 (en) 2025-06-11
WO2022256546A2 (en) 2022-12-08
AU2022283895A1 (en) 2023-12-14
EP4347811A2 (en) 2024-04-10
AU2022283895A9 (en) 2024-01-25
US20240263159A1 (en) 2024-08-08

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