AU2022264261B2 - Cell culture system and method for adjusting viable cell concentration - Google Patents

Cell culture system and method for adjusting viable cell concentration Download PDF

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Publication number
AU2022264261B2
AU2022264261B2 AU2022264261A AU2022264261A AU2022264261B2 AU 2022264261 B2 AU2022264261 B2 AU 2022264261B2 AU 2022264261 A AU2022264261 A AU 2022264261A AU 2022264261 A AU2022264261 A AU 2022264261A AU 2022264261 B2 AU2022264261 B2 AU 2022264261B2
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Australia
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cell
living
cell suspension
target
image
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AU2022264261A
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AU2022264261A1 (en
Inventor
Shota KAMEI
Takashi Morimoto
Tomoyuki Shimoda
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Fujifilm Corp
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Fujifilm Corp
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
    • C12M41/30—Means for regulation, monitoring, measurement or control, e.g. flow regulation of concentration
    • C12M41/36—Means for regulation, monitoring, measurement or control, e.g. flow regulation of concentration of biomass, e.g. colony counters or by turbidity measurements
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M23/00—Constructional details, e.g. recesses, hinges
    • C12M23/56—Floating elements
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M25/00—Means for supporting, enclosing or fixing the microorganisms, e.g. immunocoatings
    • C12M25/06—Plates; Walls; Drawers; Multilayer plates
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M27/00—Means for mixing, agitating or circulating fluids in the vessel
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M27/00—Means for mixing, agitating or circulating fluids in the vessel
    • C12M27/02—Stirrer or mobile mixing elements
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M31/00—Means for providing, directing, scattering or concentrating light
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
    • C12M41/48—Automatic or computerized control
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
    • G01N15/10—Investigating individual particles
    • G01N15/14—Optical investigation techniques, e.g. flow cytometry
    • G01N15/1429—Signal processing
    • G01N15/1433—Signal processing using image recognition

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  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Health & Medical Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Microbiology (AREA)
  • Genetics & Genomics (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Sustainable Development (AREA)
  • Analytical Chemistry (AREA)
  • Computer Hardware Design (AREA)
  • Clinical Laboratory Science (AREA)
  • Immunology (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
AU2022264261A 2021-04-28 2022-04-13 Cell culture system and method for adjusting viable cell concentration Active AU2022264261B2 (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
JP2021-076027 2021-04-28
JP2021076027 2021-04-28
PCT/JP2022/017731 WO2022230673A1 (ja) 2021-04-28 2022-04-13 細胞培養装置及び生細胞濃度調整方法

Publications (2)

Publication Number Publication Date
AU2022264261A1 AU2022264261A1 (en) 2023-11-09
AU2022264261B2 true AU2022264261B2 (en) 2024-10-24

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Family Applications (1)

Application Number Title Priority Date Filing Date
AU2022264261A Active AU2022264261B2 (en) 2021-04-28 2022-04-13 Cell culture system and method for adjusting viable cell concentration

Country Status (8)

Country Link
US (1) US20240052287A1 (https=)
EP (1) EP4317408A4 (https=)
JP (1) JPWO2022230673A1 (https=)
KR (1) KR20230158117A (https=)
CN (1) CN117337325A (https=)
AU (1) AU2022264261B2 (https=)
TW (1) TW202332759A (https=)
WO (1) WO2022230673A1 (https=)

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2015210212A (ja) * 2014-04-28 2015-11-24 横河電機株式会社 細胞生死判定システム、細胞生死判定方法
WO2018143102A1 (ja) * 2017-01-31 2018-08-09 富士フイルム株式会社 細胞培養装置、撮像ユニット及び培養監視方法

Family Cites Families (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP4744187B2 (ja) * 2005-05-10 2011-08-10 オリンパス株式会社 細胞観察装置
US10858621B2 (en) * 2014-07-22 2020-12-08 Hitachi High-Tech Corporation Cell dispersion measurement mechanism, and cell subculture system utilizing same
WO2016013394A1 (ja) 2014-07-22 2016-01-28 株式会社日立ハイテクノロジーズ 細胞数濃度調整装置およびそれを用いた自動継代培養システム
CN107075437B (zh) * 2014-10-22 2019-12-03 株式会社日立高新技术 细胞测量机构和具有该细胞测量机构的细胞培养装置以及细胞测量方法
JP6609967B2 (ja) * 2015-03-31 2019-11-27 株式会社ニコン 判定装置、判定システム、判定プログラム、及び細胞の製造方法
US10386289B2 (en) * 2016-12-23 2019-08-20 miDiagnostics NV Method and system for determining features of objects in a suspension
CN110914666A (zh) * 2017-05-19 2020-03-24 兴盛生物科技股份有限公司 用于计数细胞的系统和方法
JP7327086B2 (ja) 2019-11-05 2023-08-16 株式会社豊田自動織機 内燃機関の制御装置
AU2022263866B2 (en) * 2021-04-28 2025-08-07 Fujifilm Corporation Cell life-and-death determination method, cell life-and-death determination device, and cell life-and-death determination system

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2015210212A (ja) * 2014-04-28 2015-11-24 横河電機株式会社 細胞生死判定システム、細胞生死判定方法
WO2018143102A1 (ja) * 2017-01-31 2018-08-09 富士フイルム株式会社 細胞培養装置、撮像ユニット及び培養監視方法

Also Published As

Publication number Publication date
AU2022264261A1 (en) 2023-11-09
CN117337325A (zh) 2024-01-02
US20240052287A1 (en) 2024-02-15
EP4317408A1 (en) 2024-02-07
JPWO2022230673A1 (https=) 2022-11-03
WO2022230673A1 (ja) 2022-11-03
TW202332759A (zh) 2023-08-16
KR20230158117A (ko) 2023-11-17
EP4317408A4 (en) 2025-03-26

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