AU2021104987A4 - Banana tissue culture seedling cultivation substrate and preparation method and application thereof - Google Patents
Banana tissue culture seedling cultivation substrate and preparation method and application thereof Download PDFInfo
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- 241000234295 Musa Species 0.000 title claims abstract description 180
- 235000018290 Musa x paradisiaca Nutrition 0.000 title claims abstract description 177
- 239000000758 substrate Substances 0.000 title claims abstract description 61
- 238000002360 preparation method Methods 0.000 title claims abstract description 14
- 239000002689 soil Substances 0.000 claims abstract description 78
- 235000015097 nutrients Nutrition 0.000 claims abstract description 38
- 238000002156 mixing Methods 0.000 claims abstract description 10
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 9
- 239000002245 particle Substances 0.000 claims abstract description 5
- 235000013162 Cocos nucifera Nutrition 0.000 claims description 18
- 244000060011 Cocos nucifera Species 0.000 claims description 18
- 239000003415 peat Substances 0.000 claims description 18
- 238000000034 method Methods 0.000 claims description 12
- 241000196324 Embryophyta Species 0.000 abstract description 15
- 230000012010 growth Effects 0.000 abstract description 15
- 230000000813 microbial effect Effects 0.000 abstract description 14
- 230000002503 metabolic effect Effects 0.000 abstract description 8
- 238000003763 carbonization Methods 0.000 abstract description 5
- 230000000694 effects Effects 0.000 abstract description 5
- 238000007605 air drying Methods 0.000 abstract description 3
- 230000001580 bacterial effect Effects 0.000 abstract description 3
- 235000021015 bananas Nutrition 0.000 abstract description 3
- 230000035558 fertility Effects 0.000 abstract description 3
- 238000011282 treatment Methods 0.000 description 27
- 239000000203 mixture Substances 0.000 description 13
- 229910052799 carbon Inorganic materials 0.000 description 12
- 241000894006 Bacteria Species 0.000 description 9
- OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical compound [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 description 8
- 239000002028 Biomass Substances 0.000 description 7
- 240000000905 Nymphoides indica Species 0.000 description 6
- 235000017590 Nymphoides indica Nutrition 0.000 description 6
- 239000000463 material Substances 0.000 description 6
- 229910052757 nitrogen Inorganic materials 0.000 description 6
- 229910052698 phosphorus Inorganic materials 0.000 description 5
- 229930002875 chlorophyll Natural products 0.000 description 4
- 235000019804 chlorophyll Nutrition 0.000 description 4
- ATNHDLDRLWWWCB-AENOIHSZSA-M chlorophyll a Chemical compound C1([C@@H](C(=O)OC)C(=O)C2=C3C)=C2N2C3=CC(C(CC)=C3C)=[N+]4C3=CC3=C(C=C)C(C)=C5N3[Mg-2]42[N+]2=C1[C@@H](CCC(=O)OC\C=C(/C)CCC[C@H](C)CCC[C@H](C)CCCC(C)C)[C@H](C)C2=C5 ATNHDLDRLWWWCB-AENOIHSZSA-M 0.000 description 4
- 230000006378 damage Effects 0.000 description 4
- 239000003337 fertilizer Substances 0.000 description 4
- 244000005700 microbiome Species 0.000 description 4
- 239000000126 substance Substances 0.000 description 4
- ZCYVEMRRCGMTRW-UHFFFAOYSA-N 7553-56-2 Chemical compound [I] ZCYVEMRRCGMTRW-UHFFFAOYSA-N 0.000 description 3
- 241000186361 Actinobacteria <class> Species 0.000 description 3
- 241000233866 Fungi Species 0.000 description 3
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 description 3
- 239000011630 iodine Substances 0.000 description 3
- 229910052740 iodine Inorganic materials 0.000 description 3
- 229910052760 oxygen Inorganic materials 0.000 description 3
- 239000001301 oxygen Substances 0.000 description 3
- 230000008635 plant growth Effects 0.000 description 3
- 239000011148 porous material Substances 0.000 description 3
- 238000001179 sorption measurement Methods 0.000 description 3
- 229910052717 sulfur Inorganic materials 0.000 description 3
- 238000010998 test method Methods 0.000 description 3
- 238000013494 PH determination Methods 0.000 description 2
- 244000052616 bacterial pathogen Species 0.000 description 2
- 239000003610 charcoal Substances 0.000 description 2
- 238000002485 combustion reaction Methods 0.000 description 2
- 238000005056 compaction Methods 0.000 description 2
- 238000001816 cooling Methods 0.000 description 2
- 230000003247 decreasing effect Effects 0.000 description 2
- 238000003306 harvesting Methods 0.000 description 2
- 229910052751 metal Inorganic materials 0.000 description 2
- 239000002184 metal Substances 0.000 description 2
- 239000005416 organic matter Substances 0.000 description 2
- 238000004806 packaging method and process Methods 0.000 description 2
- 230000029553 photosynthesis Effects 0.000 description 2
- 238000010672 photosynthesis Methods 0.000 description 2
- 229910052700 potassium Inorganic materials 0.000 description 2
- 239000000725 suspension Substances 0.000 description 2
- 239000002023 wood Substances 0.000 description 2
- UHPMCKVQTMMPCG-UHFFFAOYSA-N 5,8-dihydroxy-2-methoxy-6-methyl-7-(2-oxopropyl)naphthalene-1,4-dione Chemical compound CC1=C(CC(C)=O)C(O)=C2C(=O)C(OC)=CC(=O)C2=C1O UHPMCKVQTMMPCG-UHFFFAOYSA-N 0.000 description 1
- UXVMQQNJUSDDNG-UHFFFAOYSA-L Calcium chloride Chemical compound [Cl-].[Cl-].[Ca+2] UXVMQQNJUSDDNG-UHFFFAOYSA-L 0.000 description 1
- 229910017518 Cu Zn Inorganic materials 0.000 description 1
- 102000004190 Enzymes Human genes 0.000 description 1
- 108090000790 Enzymes Proteins 0.000 description 1
- 241000223218 Fusarium Species 0.000 description 1
- ZOKXTWBITQBERF-UHFFFAOYSA-N Molybdenum Chemical compound [Mo] ZOKXTWBITQBERF-UHFFFAOYSA-N 0.000 description 1
- 241000088844 Nothocestrum Species 0.000 description 1
- 238000010521 absorption reaction Methods 0.000 description 1
- 238000009825 accumulation Methods 0.000 description 1
- 239000003463 adsorbent Substances 0.000 description 1
- 238000004458 analytical method Methods 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 229910052791 calcium Inorganic materials 0.000 description 1
- 239000001110 calcium chloride Substances 0.000 description 1
- 229910001628 calcium chloride Inorganic materials 0.000 description 1
- 235000011148 calcium chloride Nutrition 0.000 description 1
- 238000004737 colorimetric analysis Methods 0.000 description 1
- 239000002131 composite material Substances 0.000 description 1
- 229910052802 copper Inorganic materials 0.000 description 1
- 238000012364 cultivation method Methods 0.000 description 1
- 238000012258 culturing Methods 0.000 description 1
- 238000011161 development Methods 0.000 description 1
- 235000013399 edible fruits Nutrition 0.000 description 1
- 230000007613 environmental effect Effects 0.000 description 1
- XTLNYNMNUCLWEZ-UHFFFAOYSA-N ethanol;propan-2-one Chemical compound CCO.CC(C)=O XTLNYNMNUCLWEZ-UHFFFAOYSA-N 0.000 description 1
- 230000004907 flux Effects 0.000 description 1
- 239000010903 husk Substances 0.000 description 1
- 229910052742 iron Inorganic materials 0.000 description 1
- 239000007788 liquid Substances 0.000 description 1
- 229910052749 magnesium Inorganic materials 0.000 description 1
- 230000014759 maintenance of location Effects 0.000 description 1
- 229910052748 manganese Inorganic materials 0.000 description 1
- 238000005259 measurement Methods 0.000 description 1
- 229910052750 molybdenum Inorganic materials 0.000 description 1
- 239000011733 molybdenum Substances 0.000 description 1
- 238000000197 pyrolysis Methods 0.000 description 1
- 238000004064 recycling Methods 0.000 description 1
- 239000007787 solid Substances 0.000 description 1
- 239000006228 supernatant Substances 0.000 description 1
- 229910052725 zinc Inorganic materials 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G24/00—Growth substrates; Culture media; Apparatus or methods therefor
- A01G24/20—Growth substrates; Culture media; Apparatus or methods therefor based on or containing natural organic material
- A01G24/22—Growth substrates; Culture media; Apparatus or methods therefor based on or containing natural organic material containing plant material
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G22/00—Cultivation of specific crops or plants not otherwise provided for
- A01G22/05—Fruit crops, e.g. strawberries, tomatoes or cucumbers
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G24/00—Growth substrates; Culture media; Apparatus or methods therefor
- A01G24/20—Growth substrates; Culture media; Apparatus or methods therefor based on or containing natural organic material
- A01G24/22—Growth substrates; Culture media; Apparatus or methods therefor based on or containing natural organic material containing plant material
- A01G24/25—Dry fruit hulls or husks, e.g. chaff or coir
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G24/00—Growth substrates; Culture media; Apparatus or methods therefor
- A01G24/20—Growth substrates; Culture media; Apparatus or methods therefor based on or containing natural organic material
- A01G24/28—Growth substrates; Culture media; Apparatus or methods therefor based on or containing natural organic material containing peat, moss or sphagnum
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H4/00—Plant reproduction by tissue culture techniques ; Tissue culture techniques therefor
- A01H4/002—Culture media for tissue culture
-
- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05F—ORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C, e.g. FERTILISERS FROM WASTE OR REFUSE
- C05F11/00—Other organic fertilisers
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Environmental Sciences (AREA)
- Engineering & Computer Science (AREA)
- Biotechnology (AREA)
- Developmental Biology & Embryology (AREA)
- Botany (AREA)
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Cell Biology (AREA)
- Cultivation Of Plants (AREA)
Abstract
The invention provides a banana tissue culture seedling cultivation substrate and
preparation method and application thereof. The banana pseudostem biochar of the
present invention is prepared by cutting the banana pseudostems and naturally air
drying them until the water content drops below 15%, crushing them into particles
with a diameter of 2-10 mm, and processing them in a carbonization fumace.The
banana tissue culture seedling cultivation substrate is prepared by uniformly mixing
1%-3% (mass fraction) of banana pseudostem biochar and balance nutrient soil. The
application of banana pseudostem biochar can obviously optimize the metabolic
activity of banana seedling soil microbial community. A large number of bacterial
metabolic activities and biochar bring in a large number of nutrient elements, which
improve the soil fertility and significantly promote the growth of banana tissue culture
seedlings. Banana pseudostem biochar has shown a good application effect in banana
cultivation, and has a broad prospect in the cultivation and application of bananas and
other plants.
DRAWINGS
4
0
CK C1C2 C3
Treament
Figure 5
Figure 6
3
Description
4
CK C1C2 C3 Treament
Figure 5
Figure 6
Banana tissue culture seedling cultivation substrate and
preparation method and application thereof
The invention belongs to the field of preparation and application of biomass
fertilizer in agriculture, and particularly relates to a banana tissue culture seedling
cultivation substrate and a preparation method and application thereof.
Banana, as a main economic fruit, is widely planted in tropical and subtropical
areas, but its traditional cultivation method relies on a large amount of chemical
fertilizer, which often leads to the problems of destruction of soil structure, soil
compaction, decreased organic matter content and low vitality of microbial
communities. On the other hand, after harvest, a large number of banana pseudostems
piled up in the fields are easy to cause pollution, and the accumulation of banana
pseudostems with germs is easy to cause the spread of germs such as fusarium wilt.
Biochar refers to the pyrolysis of biomass into solid substances rich in carbon
elements under oxygen-free conditions. At present, the effect of biochar on soil
microbial community around banana seedling roots has not been reported.
The purpose of the invention is to solve the problems of banana soil environment
destruction and banana pseudostem pollution, and provide a banana tissue culture
seedling cultivation substrate which can reduce banana pseudostem pollution,
efficiently improve soil environment, optimize the metabolic activity of banana
seedling soil microbial community, promote the growth of banana seedlings, and is
environment-friendly and easy to operate, and the preparation method and application
thereof.
The first object of the present invention is to provide a banana pseudostem
biochar and a preparation method thereof.
The banana pseudostem biochar is characterized in that it is prepared by the
following method: the banana pseudostem is chopped and then naturally air-dried to
reduce the water content of the banana pseudostem to below 15%, and the water
content of the banana pseudostem used for ignition is reduced to below 8%; the air
dried banana pseudostem is crushed into particles with a diameter of 2-10 mm, and
then carbonized and processed into banana pseudostem biochar
The second object of the present invention is to provide a banana tissue culture
seedling cultivation substrate and a preparation method thereof.
The banana tissue culture seedling cultivation substrate is characterized in that
according to the mass fraction of 100%, it contains 1%-3% of banana pseudostem
biochar and the balance is nutrient soil.
The nutrient soil is preferably composed of peat soil and coconut coir which are
uniformly mixed according to the volume ratio of 1: 2-5.
The method for preparing the banana tissue culture seedling cultivation substrate
is characterized in that banana pseudostem biochar is added into nutrient soil to
account for 1%-3% of the total mass, and then the banana tissue culture seedling
cultivation substrate is obtained after uniform mixing.
The nutrient soil is preferably composed of peat soil and coconut coir which are
uniformly mixed according to the volume ratio of 1: 2-5.
The third object of the present invention is to provide the application of banana
pseudostem biochar in preparing banana tissue culture seedling cultivation substrate.
According to the invention, banana pseudostem biochar is prepared and applied
to the cultivation substrate of banana tissue culture seedlings. Experimental results
show that banana pseudostem biochar can promote the growth of bacteria, fungi,
actinomycetes, nitrogen-fixing bacteria and ammoniated bacteria in the cultivation
substrate, and obviously optimize the metabolic activity of banana seedling soil
microbial community. A large number of bacterial metabolic activities and banana
pseudostem biochar bring in a large number of nutrient elements, which improve the
soil fertility and significantly promote the growth of banana tissue culture seedlings
Banana pseudostem biochar has shown a good application effect in banana cultivation,
and has a broad prospect in the cultivation and application of bananas and other plants.
According to the invention, banana pseudostem are used for preparing biochar,
so that the problem of recycling a large number of pseudostem after banana
harvesting is solved, and the pollution caused by stacking a large number of banana
pseudostem in fields is avoided, and the problems of the problems of destruction of
soil structure, soil compaction, decreased organic matter content and low vitality of
microbial communities and the like caused by relying on a large amount of chemical
fertilizers in the traditional cultivation mode are solved, so that it can realize the
sustainable agricultural development with high efficiency, environmental protection,
no harm and simple and feasible operation.
Fig. 1 shows the growth of bacteria in cultivation substrate under different
treatment.
Fig. 2 shows the growth of fungi in cultivation substrate under different
treatments.
Fig. 3 shows the growth of actinomycetes in cultivation substrate under different
treatment.
Fig. 4 shows the growth of nitrogen-fixing bacteria in cultivation substrate under
different treatment.
Fig. 5 shows the growth of amonifying bacteria in cultivation substrate under
different treatment.
Fig. 6 is a scanning electron microscope photograph of microstructure of banana
pseudostem biochar.
Fig. 7 is AWCD value of soil microbial community under different treatments.
Fig. 8 shows the carbon source utilization of soil microbial community under
different treatments.
The following examples further illustrate the present invention, but do not limit it.
Example 1: preparation of banana pseudostem biochar
preparing banana pseudostem biochar
Chopping banana pseudostems, air drying naturally to reduce the water content
to 15%, and reducing the water content of banana pseudostems for ignition to 8%; the
air-dried banana pseudostems were crushed into particles with a diameter of 2-10mm
by a crusher. The banana pseudostem for ignition was put under the combustion
leaflet in the combined carbonization furnace, and it was ignited with open flame;
Then, the air-dried banana pseudostems were added into the furnace body, and the
material layer was 60 cm higher than the bumer.Feeding once every 1 hour, with a
thickness of 10-30 cm. The oxygen supply in the furnace body was controlled by the
thickness of the material layer artificially. After the banana pseudostem material was
loaded for the last time, the top cover is buckled on the top of the furnace body, and
when a large amount of steam overflows, the top cover was closed to seal the
carbonization furnace. Cooling for 12 hours, and preparing banana pseudostem
biochar; removing the top cover, removing the finished banana pseudostem biochar,
and packaging and storing to obtain the banana pseudostem biochar.
1.2 Determination of properties of banana pseudostem biochar
1.2.1 Determination of pH of banana pseudostem biochar
The method for determining pH of banana pseudostem biochar refers to
National Standard of the People's Republic of China: Test method of wood activated
carbon-determinationofpH value (GB/T 12496.7-1999). The results showed that the
pH value of banana pseudostem biochar was 10.30.
1.2.2 Determination of pH of nutrient soil and banana pseudostem biochar mixed
nutrient soil
Determination method: nutrient soil (CK) and banana pseudostem biochar mixed
nutrient soil (made up of banana pseudostem biochar and nutrient soil, in which
banana pseudostem biochar accounts for 1%, 2% and 3% of the total mass of banana
pseudostem biochar mixed nutrient soil respectively) were added into 0. 01mol-1
CaCl2 according to the ratio of soil to liquid of 1:5 to form nutrient soil suspension.
The nutrient soil suspension was oscillated on the shaker for lh, then centrifuged at
2000r • min- Ifor 10 min, and the pH of the supernatant was determined by composite
electrode.
The nutrient soil is composed of peat soil and coconut coir which are uniformly
mixed according to the volume ratio of 1: 2.
The determination results are shown in table 1:
Table 1 pH of nutrient soil and banana pseudostem biochar mixed nutrient soil
11=ng ramoof b nutrt sil (KIINZ%
FH(M7.13 7.70 7.90
1%, 2% and 3% respectively represent the proportion of banana pseudostem
biochar to the total mass of banana pseudostem biochar mixed nutrient soil.
1.2.3 Determination of fixed carbon, ash and volatile content in banana
pseudostem biochar
The determination method refers to National Standard of the People's Republic
of China: Charcoal and Charcoal Test Methods (GB/T 17664- 1999). The results
showed that the banana pseudostem biochar contained 43.38% of fixed carbon,
37.22% of ash and 19.4% of volatile content.
1.2.4 Determination of iodine adsorption value of banana pseudostem biochar
The determination method refers to the National Standard of the People's
Republic of China: Test Methods of Wood Activated Carbon •Determination of
Iodine Adsorption Value (GB/T12496.8-1999). The results showed that the iodine
adsorption value of banana pseudostem biochar was 159.54 mg/g.
1.2.5 Determination of the contents of C, N, S and P in banana pseudostem
biochar
Determination method: C, N and S elements were determined by CNS element
analyzer. The content of P was determined by molybdenum blue colorimetry. The
determination results are shown in table 2.
Table 2 Contents of C, N, S and P in banana pseudostem biochar
Element (7 N S P
content ( % ) 44.6 1.65 0.59 0.59
1.2.6 Determination of metal element content in banana pseudostem biochar
Determination method: the contents of k, Ca, Mg, Fe, Cu, Zn and Mn in banana
pseudostem biochar were determined by element atomic absorption spectrometer. The
determination results are shown in table 3.
Table 3 Metal element content of banana pseudostem biochar
Element K CA Mg F Cu Zn Mn
Content 1 % ) 3186 053 106 0 68 0103 0.05 0,108
1.2.7 Specific surface area and pore structure parameters of banana pseudostem
biochar
Determination method: The specific surface area, micropores and chemical
adsorbents were used to determine the content. The determination results are shown in
table 4.
Table 4 Specific surface area and pore structure parameters of banana
pseudostem biochar
BET specifIC T-Hot T-Plotexralegal mi Total P T-Hot Aveage po diameter smfm area Fahmhole volume Fahm hole
3 (n~ 8 Are ('1Vueiia 1n'lgJ (rrn 1 7q) Voh~ l) (un aiea
6.7 td 9 6 , o.00 OI172 l0.11522
Example 2: Preparation of banana tissue culture seedling cultivation substrate
2.1 Mix peat soil and coconut coir evenly according to the volume ratio of 1: 2 to
prepare nutrient soil, add banana pseudostem biochar to make it account for 1% of the
total mass of banana tissue culture seedling cultivation substrate, and mix evenly to
obtain 1% banana tissue culture seedling cultivation substrate.
2.2 Mix peat soil and coconut coir evenly according to the volume ratio of 1: 2 to
prepare nutrient soil, add banana pseudostem biochar to make it account for 2% of the
total mass of banana tissue culture seedling cultivation substrate, and mix evenly to
obtain 2% banana tissue culture seedling cultivation substrate.
2.3 Mix peat soil and coconut coir evenly according to the volume ratio of 1: 2 to
prepare nutrient soil, add banana pseudostem biochar to make it account for 3% of the
total mass of banana tissue culture seedling cultivation substrate, and mix evenly to
obtain 3% banana tissue culture seedling cultivation substrate.
Example 3:
3.1 Determination of banana plant biomass in each treatment group
Under normal cultivation and management conditions, take banana roots, stems
and leaves (there are 4 banana plants in each group) from the control group (CK,
whose cultivation substrate is nutrient soil prepared by uniformly mixing peat soil and
coconut coir according to the volume ratio of 1: 2) and the treatment group (1%, 2%,
3%, whose cultivation substrate is 1%, 2% and 3% of banana tissue culture seedling
cultivation substrate of Example 2) that were 3 months old, deactivate enzymes at
105 C, dry them to constant weight at 80C .The results showed that the biomass
content of banana plants in the treatment group was significantly higher than that in
the control group. The results are shown in Table 5.
Table 5 banana biomass under different treatments
Leaf weight Stem weight Rootweight Total weight CK 12,4 717 83 2917 Y % 15.96 i1.19 9)A5 3. 2% 20,57 13.4 10-8 44-5
CK represents nutrient soil prepared by mixing peat soil and coconut coir
uniformly according to the volume ratio of 1: 2, and 1%, 2% and 3% respectively
represent 1%, 2% and 3% of banana tissue culture seedling cultivation substrate in
Example 2.
3.2 Determination of chlorophyll content of bananas grown under different
treatment conditions
Under normal cultivation and management conditionstake the banana plant
leaves from the control group (CK, cultivation substrate is nutrient soil prepared by
mixing peat soil and coconut coir uniformly according to the volume ratio of 1: 2) and
the treatment group (1%, 2%, 3%, whose cultivation substrate is 1%, 2% and 3% of
banana tissue culture seedling cultivation substrate of Example 2) respectively that
were 3 months old,chlorophyll content in banana leaves with different treatments was
determined by acetone-ethanol mixture method.
Results is as shown in Table 6, the chlorophyll content in leaves of control group
was significantly higher than that of banana tissue culture seedling cultivation
substrate treatment group . Therefore, in the same growth period, the photosynthesis
rate of banana plants in the control group is higher, while the biomass of banana
plants in the treatment group is higher and grows better when the photosynthesis rate
is lower, which indicates that banana seedlings in the treatment group absorb more
nutrients beneficial to plant growth from the cultivation substrate.
Table 6 Chlorophyll content of banana under different treatments
'K 11 2%&'
CoDntEntmi L4i A 1,44 k405
CK represents nutrient soil prepared by mixing peat soil and coconut coir
uniformly according to the volume ratio of 1: 2, and 1%, 2% and 3% respectively
represent 1%, 2% and 3% of banana tissue culture seedling cultivation substrate in
Example 2.
Example 4: Effect of banana tissue culture seedling cultivation substrate on soil
microorganism quantity of banana seedling
Four experimental groups were designed: nutrient soil (CK) prepared by mixing
peat soil and coconut coir uniformly according to the volume ratio of 1: 2. As a
cultivation substrate,1% of banana tissue cultivation substrate (Cl, 1% in the table),
2% of banana tissue cultivation substrate (C2, 2% in the table) and 3% of banana
tissue cultivation substrate (C3, 3% in the table), there are 4 treatments in total, each
treatment repeats 5 pots, each pot has 1 banana seedling, and there are 20 pots in total.
After planting banana seedlings for 3 months, Five evenly distributed points were
selected from the circumference 6 ~ 7 cm away from the main stem of the plant, and
8g of soil samples were collected from each point about 3 ~ 4 cm away from the
surface. Five samples of the same treatment were uniformly mixed for soil
microorganism determination.
The experimental results showed that the addition of banana pseudostem biochar
with different contents promoted the growth of bacteria, fungi, actinomycetes,
nitrogen-fixing bacteria and ammonifying bacteria in soil to varying degrees after
three months of banana seedling cultivation in four experimental groups. The results
are shown in Figures 1, 2, 3, 4 and 5 respectively. A large number of bacterial
metabolic activities and banana pseudostem biochar brought in a large number of
nutrient elements, and also improved the soil fertility. By scanning the microscopic
structure of banana pseudostem biochar with electron microscope, we found that
banana pseudostem biochar has rich pore structure, as shown in Figure 6. Therefore,
after being added to the soil, the fertilizer retention capacity and oxygen flux of the
soil were obviously optimized, and then the growth of microorganisms was promoted.
Continuing to study the microbial community function with Biolog microplate, it was
found that the AWCD of soil microbial community added with banana pseudostem
biochar was higher than CK in each period, and the AWCD value of microbial
community increased with the increase of biochar amount in the same period, as
shown in Figure 7. Due to AWCD can reflect the diversity of the total capacity and
function of different microbial communities for carbon source utilization, the practical
results showed that the addition of banana pseudostem biochar promoted the
metabolic capacity of banana seedling soil microbial community, promoted the
community abundance and evenness index, and changed the utilization capacity of
different carbon sources. The analysis results are shown in Figure 8.
Example 5: Effect of banana tissue culture seedling cultivation substrate on
banana plant growth
It can be seen from Example 4 that banana pseudostem biochar can increase the
number of microorganisms in the soil. After the banana seedlings grow for three
months, we measured the plant height, stem circumference (upper, middle and lower),
number of green leaves, maximum leaf length and width of 5 banana seedlings in
each treatment, as shown in Table 7. The contents of C, N, K and P in leaves of each
treatment group were determined, as shown in Table 8.
Table 7 Growth of banana seedlings in different treatment groups
Different Measurement index Plant 1 Plant 2 Plant3 Plant 4 Plant 5 Average treatment
CK ( No Plant height biochar) kI4 46A 4 l] Stem circumference (upper) Stem circumference (middle) 4. 34 a4g Jgg Stem circumference (lower) 7743 5.jI 43 512A Green leaf number Maximum leaf length
Maximum leaf width
1% of Plant height banana 541 .49 441 44 175V tissue Stem circumference culture (upper) seedling 4h cultivation Stem circumference substrate (middle) k t11 Stem circumference a (lower) - In 614A Greenleafnumber Maximum leaf 141 length** Maximum leaf N21 width
2% of Plant height banana tissue Stem culture circumference (upper) seedling 5 4 4 149 3. 1"t€.I16 E4 cultivation Stem substrate circumference (middle) Stem circumference (lower )
Green leaf number
Maximum leaf length 4 e115 2x 14511 Maximum leaf width 3% of Plant height banana I4 7 47 I tissue Stem culture circumference (upper) 4 771. seedling cultivation Stem substrate circumference (middle) 4 -4 Stem circumference (lower) 6k Ll e1A Green leaf number M I '
Maximum leaf length '4 le. Maximum leaf width - I17? ' I AI L I1Y l
1%, 2% and 3% of banana tissue culture seedling cultivation substrate refer to
1%, 2% and 3% of banana tissue culture seedling cultivation substrate in Example
2,respectively.
Table 8 Comparison of C, N, K, P Contents in Leaves of Plants in Different
Treatment Groups
Diffrt treannent C N K P CK 36 L3M% 2.95% 0.19%
1% M8.8% IW 48% 911% 0.27%
2% 37.39% 1.29% 940% 0.31%
3% 388`% L37% 739% 0,5%
Note: The data are the weight percentage of elements and dry leaves. 1%, 2%
and 3% respectively refer to 1%, 2% and 3% of banana tissue culture seedling
cultivation substrate in Example 2. CK refers to the nutrient soil prepared by
uniformly mixing peat soil and coconut husk according to the volume ratio of 1: 2.
The experimental results showed that the growth of banana tissue culture
seedlings was significantly promoted after three months of banana tissue culture
seedling with banana pseudostem biochar. The plant biomass of banana seedlings
increased significantly and the contents of C, N, P, especially K in leaves were
increased with different amounts of banana pseudostem biochar.
Example 6: Preparation of banana pseudostem biochar and banana tissue culture
seedling cultivation substrate
6.1 Preparation of banana pseudostem biochar
Chopping banana pseudostems, air drying naturally to reduce the water content
to 11%, and reducing the water content of banana pseudostems for ignition to 6%; the
air-dried banana pseudostems were crushed into particles with a diameter of 2-10mm
by a crusher. The banana pseudostem for ignition was put under the combustion
leaflet in the combined carbonization furnace, and it was ignited with open flame;
then, the air-dried banana pseudostems were added into the furnace body, and the
material layer was 60 cm higher than the burner. Feeding once every 1 hour, with a
thickness of 10-30 cm. The oxygen supply in the furnace body was controlled
artificially by the thickness of the material layer. After the banana pseudostem
material was loaded for the last time, the top cover was buckled on the top of the
furnace body, and when a large amount of steam overflowed, the top cover was closed
to seal the carbonization furnace. After cooling for 12 hours,preparing banana
pseudostem biochar; removing the top cover, removing the finished banana
pseudostem biochar, and packaging and storing to obtain the banana pseudostem
biochar.
6.2 Preparation of banana tissue culture seedling cultivation substrate
I: evenly mix peat soil and coconut coir according to the volume ratio of 1: 5 to
prepare nutrient soil, add banana pseudostem biochar prepared in the above step 1 to
make it account for 1% of the total mass of banana tissue culture seedling cultivation
substrate, and evenly mix to obtain 1% of banana tissue culture seedling cultivation
substrate.
II, uniformly mix peat soil and coconut coir according to the volume ratio of 1: 5
to prepare nutrient soil, add the banana pseudostem biochar prepared in the step 1 to
make it account for 2% of the total mass of the banana tissue culture seedling
cultivation substrate, and uniformly mix to obtain 2% of the banana tissue culture
seedling cultivation substrate.
III, uniformly mix peat soil and coconut coir according to the volume ratio of 1:
to prepare nutrient soil, add the banana pseudostem biochar prepared in the step 1 to
make it account for 3% of the total mass of the banana tissue culture seedling
cultivation substrate, and uniformly mix to obtain 3% of the banana tissue culture
seedling cultivation substrate.
The banana tissue culture seedling cultivation substrate obtained by the above
methods I, II and III was used as the treatment group, and peat soil and coconut coir
were mixed evenly according to the volume ratio of 1: 5 to prepare nutrient soil as the
control group. After three months of culturing the banana tissue culture seedling, the
results showed that the banana tissue culture seedling cultivation substrate added with
banana pseudostem biochar promoted the metabolic capacity of soil microbial
community of banana seedling and significantly promoted the plant growth of banana
tissue culture seedling.
From the above examples, it can be seen that adding banana pseudostem biochar
to the cultivation substrate can obviously promote the growth of banana tissue culture
seedlings.
Claims (2)
1. A cultivation substrate for banana tissue culture seedlings is characterized by
containing 1%-3% of banana pseudostem biochar and the balance nutrient soil
according to the mass fraction of 100%; the banana pseudostem biochar is prepared
by the following method: the banana pseudostem is chopped and then naturally air
dried to reduce the water content of the banana pseudostem to below 15%, and the
water content of the banana pseudostem used for ignition is reduced to below 8%; the
air-dried banana pseudostem is crushed into particles with a diameter of 2-10 mm, and
then carbonized and processed into banana pseudostem biochar; the nutrient soil is
formed by uniformly mixing peat soil and coconut coir according to the volume ratio
of 1: 2-5.
2. The preparation method of banana tissue culture seedling cultivation substrate
according to claim is characterized in that banana pseudostem biochar is added into
nutrient soil to account for 1%-3% of the total mass, and then evenly mixed to obtain
the banana tissue culture seedling cultivation substrate; the nutrient soil is formed by
uniformly mixing peat soil and coconut coir according to the volume ratio of 1: 2-5.
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| CN114145177A (en) * | 2021-12-09 | 2022-03-08 | 西藏自治区农牧科学院蔬菜研究所 | Planting method of bananas |
| CN115486335A (en) * | 2022-11-03 | 2022-12-20 | 中国农业科学院都市农业研究所 | Rapid propagation and generation-adding method for tomatoes |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN114145177A (en) * | 2021-12-09 | 2022-03-08 | 西藏自治区农牧科学院蔬菜研究所 | Planting method of bananas |
| CN115486335A (en) * | 2022-11-03 | 2022-12-20 | 中国农业科学院都市农业研究所 | Rapid propagation and generation-adding method for tomatoes |
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