AU2021104615A4 - Device and method for germination of highland barley with Γ-aminobutyric acid enriched - Google Patents
Device and method for germination of highland barley with Γ-aminobutyric acid enriched Download PDFInfo
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G31/00—Soilless cultivation, e.g. hydroponics
- A01G31/02—Special apparatus therefor
- A01G31/06—Hydroponic culture on racks or in stacked containers
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01C—PLANTING; SOWING; FERTILISING
- A01C1/00—Apparatus, or methods of use thereof, for testing or treating seed, roots, or the like, prior to sowing or planting
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01C—PLANTING; SOWING; FERTILISING
- A01C1/00—Apparatus, or methods of use thereof, for testing or treating seed, roots, or the like, prior to sowing or planting
- A01C1/02—Germinating apparatus; Determining germination capacity of seeds or the like
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01C—PLANTING; SOWING; FERTILISING
- A01C1/00—Apparatus, or methods of use thereof, for testing or treating seed, roots, or the like, prior to sowing or planting
- A01C1/08—Immunising seed
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G31/00—Soilless cultivation, e.g. hydroponics
- A01G31/02—Special apparatus therefor
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L7/00—Cereal-derived products; Malt products; Preparation or treatment thereof
- A23L7/20—Malt products
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B08—CLEANING
- B08B—CLEANING IN GENERAL; PREVENTION OF FOULING IN GENERAL
- B08B3/00—Cleaning by methods involving the use or presence of liquid or steam
- B08B3/02—Cleaning by the force of jets or sprays
- B08B3/022—Cleaning travelling work
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
- Y02P60/00—Technologies relating to agriculture, livestock or agroalimentary industries
- Y02P60/20—Reduction of greenhouse gas [GHG] emissions in agriculture, e.g. CO2
- Y02P60/21—Dinitrogen oxide [N2O], e.g. using aquaponics, hydroponics or efficiency measures
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- Environmental Sciences (AREA)
- Health & Medical Sciences (AREA)
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- Nutrition Science (AREA)
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Food Science & Technology (AREA)
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- Physiology (AREA)
- Pretreatment Of Seeds And Plants (AREA)
Abstract
OF THE DISCLOSURE (Figure 1)
The present disclosure a device and a method for germination of highland barley
with y-aminobutyric acid enriched and belongs to the technical field of deep processing
of agricultural products. The present disclosure employs the combined treatment of
steam blanching sterilization, gradient heating germination, high temperature and low
oxygen enrichment to realize GABA enrichment. Steam blanching sterilization treatment
can reduce bacterial growth and ensure product safety; gradient heating germination
provides a material basis for GABA enrichment by promoting protein degradation into
free amino acids; high temperature and low oxygen enrichment can activate glutamate
decarboxylase through environmental stress, achieve GABA enrichment. The supporting
equipment system includes a feeding hopper, a grain cleaning machine, a steam sterilizer,
a conveying device, a germination bin, an enrichment bin, and drying equipment, which
provide equipment support for the pollution-free, additive-free, and industrialized
production of GABA highlandbarley. The GABA content of the product prepared by
adopting the technology of the present disclosure is 27.45-53.56 mg/100g.
1/4
59
FIG.1
2-1
2-2
-2-4
2FIG.2
FIG.2
Description
1/4
59
FIG.1
2-1
2-2
-2-4
2FIG.2
FIG.2
[0001] The present disclosure relates to the technical field of deep processing of
agricultural products, in particular to a device and a method for germination of highland
barley with y-aminobutyric acid enriched.
[0002] y-aminobutyric acid (GABA) is a non-protein amino acid widely found in
animals, plants and microorganisms. As an inhibitory neurotransmitter, GABA has a
variety of physiological effects, such as lowering blood pressure, lowering lipids,
lowering blood sugar, anti-cancer, diuresis, calming and so on. Therefore, foods rich in
GABA are increasingly popular among consumers.
[0003] The existing methods for germination and enrichment of GABA are based on
soaking germination, supplemented by different types of culture media and stress
treatments to promote the enrichment of GABA. However, there are still some problems:
1) The use of soaking to germinate will lead to waste of water resources and easily lead
to the growth of bacteria; 2) The use of chemical reagents containing sodium glutamate,
citric acid, calcium chloride and other chemical reagents does not conform to the modern
demands of non-additive, less-addition 3) The use of pulsed light, modified atmosphere
packaging and other technologies to assist germination, although it can promote GABA
enrichment, but the processing cost is too high and it is difficult to achieve industrial
application. Therefore, the existing technology cannot meet the requirements of large
scale and effective GABA enrichment in industrial production.
[0004] The purpose of the present disclosure is to provide a device and method for
germination of highland barley with y-aminobutyric acid enriched. The device and
method of the present disclosure can efficiently enrich y-aminobutyric acid on a large
scale.
[0005] In order to achieve the above-mentioned purpose of the present disclosure, the
present disclosure provides the following technical solutions:
[0006] The present disclosure provides a device for germination of highland barley
with y-aminobutyric acid enriched, which is connected with a hopper 1, a grain cleaning
machine 2, a steam sterilizer 3, a germination cabinet 5, and an enrichment bar along the
conveying direction of the highland barley grains, warehouse 7 and dryer 9;
[0007] The feed hopper 1, the grain cleaning machine 2, the steam sterilizer 3 and the
germination cabinet 5 are connected through a first conveying device 4; the germination
cabinet 5 and the enrichment bin 7 are connected through a second conveying device 6;
The enrichment bin 7 and the dryer 9 are communicated with each other through a third
conveying device 8.
[0008] Preferably, the grain cleaning machine 2 includes a water tank 2-1, an atomizing
spray head 2-2, a cleaning chamber 2-3, a compressed air inlet 2-4, a drain 2-5, a
conveying track 2-6 and the air compressor 2-7; the water inlet of the atomizing spray
head 2-2 is connected to the water tank 2-1, and the atomizing spray head 2-2 is arranged
at the top of the cleaning chamber 2-3; The cleaning chamber 2-3 is provided with a
conveying track 2-6 for conveying highland barley grains; the bottom of the cleaning bin
2-3 is provided with a compressed air inlet 2-4 and a drain 2-5; the air compressor 2-7 is
connected to the compressed air inlet 2-4 to deliver compressed air to the cleaning
chamber 2-3.
[0009] Preferably, the steam sterilizer 3 includes a steam generator 3-1, a steam inlet 3 2, a sterilization box 3-3, a conveying track 3-4, a grain layer thickness control ruler 3-5, and steam condensate recycle assembly 3-7;
[0010] The bottom of the sterilization box 3-3 is provided with a steam inlet 3-2; the steam generator 3-1 is connected to the steam inlet 3-2 through a pipe, and steam is introduced into the sterilization box 3-3; The bottom of the sterilization box 3-3 is also provided with a water outlet 3-8, the water outlet 3-8 is connected to the steam generator 3-1 through a pipe; the steam condensate recycle assembly 3-7 is connected to the steam generator 3-1 through a pipe ; the inside of the sterilization box 3-3 is provided with a conveying track 3-4 and a grain layer thickness control ruler 3-5; the grain layer thickness control ruler 3-5 is arranged at the entrance of the conveying track 3- 4 ; the upper wall of the sterilization box 3-3 is provided with an air-vent 3-6; the air-vent 3-6 is connected to the steam condensate recycle assembly 3-7 through a pipe.
[0011] Preferably, the germination cabinet 5 includes a plurality of vertically distributed germination units 5-7; the germination cabinet 5 is arranged in a germination chamber that includes an air conditioning system and a temperature and humidity sensor;
[0012] Each germination unit 5-7 is provided with several layers of liquid collection tray 5-2, an atomizing spray system 5-1 and an ultraviolet sterilization system 5-3; the liquid collection tray 5-2 is provided with a drainage system 5-5. The atomized spray system 5-1 and the ultraviolet sterilization system 5-3 are fixed on the top of the germination cabinet or the lower part of the liquid collection tray 5-2 of the germination unit 5-7 on the upper layer;
[0013] In each germination unit 5-7, the space between the liquid collection pan 5-2, the spray system and the ultraviolet sterilization system is provided with a germination tray track 5-4; for placing the germination tray 5-6.
[0014] Preferably, the enrichment bin 7 comprises a steam generator 7-1, a vacuum
peristaltic pump 7-2, a thermal insulation interlayer 7-3, a coil heating tube 7-4, an
enrichment box 7-5, and a pressure sensor 7-6, temperature sensor 7-7 and sealing cover
7-8;
[0015] The thermal insulation interlayer 7-3 is fixed to the outer side wall of the
enrichment box 7-5; the thermal insulation interlayer is provided with a tube-coil heating
tube 7-4; the tube-coil heating tube 7-4 and a steam generator 7 -1 is connected by a pipe;
the inner side wall of the enrichment box 7-5 is fixed with a pressure sensor 7-6 and a
temperature sensor 7-7; the upper part of the enrichment box 7-5 is provided with a
sealing cover 7-8; The outer side wall of the enrichment box 7-5 is provided with an
observation window 7-9; the bottom of the enrichment box 7-5 is provided with a
discharge port 7-10 and an air-vent 7-11; The air-vent 7-11 and the vacuum peristaltic
pump 7-2 are connected by a pipe.
[0016] The present disclosure also provides a method for germination of highland
barley with y-aminobutyric acid enriched based on the device of any one of claims 1 to
, comprising the following steps:
1) Fill the highland barley grains in the feed hopper (1);
2) Transfer the highland barley grains from the first conveying device (4) to the grain
cleaning machine (2), and start the grain cleaning machine to clean the highland barley
grains to obtain clean highland barley grains;
3) Transfer the clean highland barley grains to the steam sterilizer (3) by the first
conveying device (4), and start the steam sterilizer (3) to sterilize the clean highland
barley grains to obtain aseptic highland barley grains;
4) Transfer the aseptic highland barley seeds to the germination tray (5-6) in the
germination cabinet (5) through the first transfer device (4), carry out afirst germination
and a second germination in sequence to obtain the highland barley sprouts; A
temperature of the first germination is 13-18°C and a time is 0.1-30h; A temperature of
the second germination is 28-32C and a time is 0.1-30h;
) Transfer the highland barley sprouts to the enrichment bin (7) through the second
conveying device (6), and after being evacuated by a vacuum peristaltic pump, they are
enriched at45-55C for 0.1-30hto obtain enriched sprouts;
6) Convey the enriched sprouts to the dryer (9) through the third conveying device (8),
and dry at 55-65C for 10-12 hours to obtain highland barley sprouts enriched in y
aminobutyric acid.
[0017] Preferably, during the first germination instep 4), the thickness of the highland
barley grain layer is 0.5-1.0 cm, and spray humidification every 3 to 5 hours, an amount
of water added each time accounts for 8%-12% of the weight of aseptic highland barley
grains, a ventilation interval is 6-8h, and each ventilation time is 10-15min.
[0018] Preferably, during the second germination instep 4), a thickness of the highland
barley grain layer is 0.5-1.0 cm, and spray humidification every 5-7h, an amount of water
added each time accounts for 5% to 8% of the weight of the aseptic highland barley
grains, a ventilation interval is 3 to 4 hours, and each ventilation time is 10 to 15 minutes.
[0019] Preferably, instep 5), the filling amount of the highland barley sprouts accounts
for 3/4 to 4/5 of the volume of the enrichment bin.
[0020] The beneficial effects of the present disclosure: The present disclosure provides
a device for germination of highland barley with y-aminobutyric acid enriched, which is
sequentially connected with a feed hopper 1, a grain cleaning machine 2, a steam sterilizer 3, a germination cabinet 5, an enrichment bin 7, and the dryer 9; the feed hopper 1, the grain cleaning machine 2, the steam sterilizer 3, and the germination cabinet 5 are connected through the first conveying device 4; the germination cabinet 5 and the enrichment bin 7 is connected through a second conveying device (6); the enrichment bin 7 and the dryer 9 are connected through a third conveying device 8. Based on the above device, the present disclosure provides a method for enrichingy-aminobutyric acid for the germination of highland barley, which can realize the enrichment of highland barley GABA.
[0021] In the present disclosure, the steam sterilizer 3 is used to sterilize the cleaned highland barley grains, which can effectively reduce the influence of bacterial reproduction on GABA enrichment; the germination cabinet 5 is used for gradient heating germination and adopts a combination strategy of low temperature germination and high temperature germination. The low-temperature treatment in the germination stage reduces the growth of bacteria, and the high-temperature treatment in the germination stage is good for the conversion of substances inside the seeds promotes the degradation of proteins into free amino acids, and provides a material basis for the next GABA enrichment. The enrichment chamber 7 is used for enrichment, which provides a high temperature and low oxygen stress environment for germinating highland barley, promotes the activation of glutamate decarboxylase, catalyzes the decarboxylation reaction of free glutamate, generates GABA, and realizes GABA enrichment; Drying the enriched germination at 55 -65C reduces the Maillard reaction between GABA and reducing sugars and reduces the decrease in GABA content. The content of y-aminobutyric acid in the highland barley sprouts prepared by using the device and method of the present disclosure is as high as 53.56±1.51 mg/100g.
[0022] The present disclosure is a device and method for germination of highland barley with y-aminobutyric acid enriched, which is beneficial to the pollution-free, additive-free and industrialized production of GABA highland barley.
[0023] Figure 1 is a schematic diagram of the structure of the device for germination
of highland barley with y-aminobutyric acid enriched in the present disclosure; wherein,
the feeding hopper 1, the grain cleaning machine 2, the steam sterilizer 3, the first
conveying device 4, the germination cabinet 5, The second conveying device 6, the
enrichment bin 7, the third conveying device 8 and the dryer 9;
[0024] Figure 2 is a schematic diagram of the structure of the grain cleaning machine
of the present disclosure; where the water tank 2-1, the atomizing spray head 2-2, the
cleaning chamber 2-3, the compressed air inlet 2-4, the drain 2-5, and the transmission
track 2-6 and air compressor 2-7;
[0025] Fig. 3 is a schematic diagram of the structure of the steam sterilizer in the present
disclosure; wherein, the steam generator 3-1, the steam inlet 3-2, the sterilization box 3
3, the conveying track 3-4, and the grain layer thickness control ruler 3-5, air-vent 3-6,
steam condensate recycle assembly 3-7 and water outlet 3-8
[0026] Figure 4 is a schematic diagram of the structure of the germination cabinet in
the germination chamber of the present disclosure; wherein, the atomization spray
system 5-1, the body collection tray 5-2, the ultraviolet sterilization system 5-3, the
germination tray track 5-4, and the drainage system 5-5, germination tray5-6 and
germination unit 5-7;
[0027] Figure 5 is a schematic diagram of the structure of the enrichment bin in the
present disclosure; wherein the steam generator 7-1, the vacuum peristaltic pump 7-2,
the thermal insulation interlayer 7-3, the coil heating tube 7-4, and the enrichment box
7-5, pressure sensor 7-6, temperature sensor 7-7, sealing cover 7-8, observation window
7-9, discharge port 7-10 and air-vent 7-11;
[0028] Fig. 6 is a process flow diagram of the method for germination of highland barley with y-aminobutyric acid enriched of the present disclosure.
[0029] The present disclosure provides a device for germination of highland barley with y-aminobutyric acid enriched which is sequentially connected with a feed hopper 1, a grain cleaning machine 2, a steam sterilizer 3, a germination cabinet 5, and an enrichment bin 7 and a dryer 9; the feeding hopper 1, the grain cleaning machine 2, the steam sterilizer 3 and the germination cabinet 5 are connected through a first conveying device 4; the germination cabinet 5 and the enrichment bin 7 are connected through a second conveying device 6: The enrichment bin 7 and the dryer 9 are connected through a third conveying device 8.
[0030] A schematic diagram of the structure of the device refers to FIG.1.
[0031] In the present disclosure, the present disclosure has no special requirements on the feed hopper, and a conventional feed hopper can be used; the feed hopper is capable of adjusting the feeding speed.
[0032] In the present disclosure, the grain cleaning machine 2 includes a water tank 2 1, an atomizing spray head 2-2, a cleaning chamber 2-3, a compressed air inlet 2-4, a drain 2-5, a conveying track 2 -6 and a air compressor 2-7; the atomization spray head 2-2 is arranged in the upper part of the cleaning chamber 2-3; the water inlet of the atomization spray head 2-2 is connected to the water tank 2-1, the atomization spray head 2-2 is arranged at the top of the cleaning chamber 2-3; the inside of the cleaning chamber 2-3 is provided with a conveying track 2-6 for conveying highland barley grains; the bottom of the cleaning chamber 2-3, a compressed air inlet 2-4 and a drain 2-5 are respectively provided; the air compressor 2-7 is connected to the compressed air inlet 2 4 to deliver compressed air to the cleaning chamber 2-3. In the present disclosure, the schematic diagram of the structure of the grain cleaning machine 2 is shown in FIG. 2. In the present disclosure, the air compressor 2-7 delivers compressed air to the cleaning chamber 2-3, and during the cleaning process, it promotes the floating of grains and improves the cleanliness of cleaning.
[0033] In the present disclosure, the conveying track 2-6 is matched with the first conveying device 4 and the drainage port; the compressed air discharged from the air compressor 2-7 can cause the highland barley grains to float up, and in full contact to the sprayed clean water from the atomization spray head 2-2, to realize the pneumatic spray cleaning of the seeds. The grain cleaning time is adjustable by the operating speed of the conveyor. The sewage generated during the cleaning process is discharged from the water outlet 2-5 at the lower part of the seed cleaning machine 2.
[0034] In the present disclosure, the steam sterilizer 3 includes a steam generator 3-1, a steam inlet 3-2, a sterilization box 3-3, a conveying track 3-4, a grain layer thickness control ruler 3-5, an air-vent 3-6, a steam condensate recycle assembly 3-7, and a water outlet 3-8; the bottom of the sterilization box 3-3 is provided with a steam inlet 3-2; the steam generator 3-1 is connected to steam inlet 3-2through a pipe, steam is introduced into the sterilization box 3-3; the bottom of the sterilization box 3-3 is also provided with a water outlet 3-8, and the water outlet 3-8 communicates with the steam generator 3-1 through a pipe. The steam condensate recycle assembly 3-7 communicates with the steam generator 3-1 through a pipe; the sterilization box 3-3 is provided with a conveying track 3-4 and a grain layer thickness control ruler 3-5; The grain layer thickness control ruler 3-5 is set at the entrance of the conveying track 3-4; the upper wall of the sterilization box 3-3 is provided with an air-vent 3-6; the air-vent 3-6 is connected to steam condensate recycle assembly 3-7 through pipes. In the present disclosure, the structure diagram of the steam sterilizer 3 is shown in FIG. 3.
[0035] In the present disclosure, only the inlet and outlet of the conveying device are reserved in the steam sterilizer, and the rest are sealed to prevent the steam from leaking out; the water outlet 3-8 of the steam condensate recycle assembly 3-7 and the steam generator water inlet are connected to ensure that the heat energy in the condensed water and residual steam generated during the sterilization process is reused. During the specific implementation of the present disclosure, the sterilization time is adjusted by adjusting the operating speed of the conveying device according to the grain size and the thickness of the grain layer.
[0036] In the present disclosure, the germination cabinet 5 preferably includes a
number of vertically distributed germination units 5-7, and the number of germination
units 5-7 in each germination cabinet 5 is preferably 2; the germination cabinet 5 is
arranged in the germination chamber including an air conditioner system and a
temperature and humidity sensor, the air conditioning system and temperature and
humidity sensor can realize the temperature control of the germination chamber and the
ventilation and exhaust C0 2 ;
[0037] Each germination unit 5-7 is provided with several layers of liquid collection
tray 5-2, an atomizing spray system 5-1 and an ultraviolet sterilization system 5-3; the
liquid collection tray 5-2 is provided with a drainage system 5-5. The atomization spray
system 5-1 and the ultraviolet sterilization system 5-3 are fixed on the top of the
germination cabinet or the lower part of the liquid collection tray 5-2 of the germination
unit 5-7 on the upper layer. In the present disclosure, the structure diagram of the
germination cabinet 5 is shown in FIG. 4. In the present disclosure, the width of the
germination tray 5-6 is the same as the width of the first conveying device 4 to ensure
that the sterilized seeds can be smoothly spread on the germination tray 5-6; the bottom
of the germination tray 5-6 is preferably a perforated screen, which can ensure that the
excess water in the germination process is drained to the liquid collecting tray, preventing
the seeds from being soaked and affecting the germination; the bottom of the germination
unit 5-7 has a movable wheel matched with the germination tray track to ensure the
germination tray to move smoothly on the germination cabinet.
[0038] In the present disclosure, the enrichment bin 7 includes a steam generator 7-1,
a vacuum peristaltic pump 7-2, a thermal insulation interlayer 7-3, a tube-coil heating
tube 7-4, an enrichment box 7-5, and a pressure sensor 7-6, temperature sensor 7-7,
sealing cover 7-8, observation window 7-9, discharge port 7-10 and air-vent 7-11; the
thermal insulation interlayer 7-3 is fixed to the outer side wall of the enrichment box 7
; a tube-coil heating tube 7-4 is provided in the thermal insulation interlayer; the tube
coil heating tube 7-4 and the steam generator 7-1 are connected by a pipe; The inner wall
of the enrichment box 7-5 is fixed with a pressure sensor 7-6 and a temperature sensor
7-7; the upper part of the enrichment box 7-5 is provided with a sealing cover 7-8; the
outer side wall of the enrichment box 7-5 is provided with an observation window 7-9;
the bottom of the enrichment box 7-5 is provided with a discharge port 7-10 and an air
vent 7-11; the air-vent 7-11 and a vacuum peristaltic pump 7-2 are connected by a pipe.
In the present disclosure, the structure diagram of the germination cabinet 5 is shown in
FIG. 4.
[0039] In the present disclosure, the bottom of the enrichment bin 7 is provided with
an air-vent 7-11, which is connected with the vacuum peristaltic pump 7-2, and
cooperates with the sealing cover 7-8 on the upper part of the germination bin to realize
the construction of vacuum and low oxygen environment in the enrichment bin. The
enrichment bin has a thermal insulation interlayer 7-3, and a tube-coil heating tube 7-4
connected to the steam generator 7-1 is arranged in the thermal insulation interlayer 7-3
to ensure the establishment of a high temperature environment in the enrichment chamber.
A pressure sensor 7-6 and a temperature sensor 7-7 are arranged inside the enrichment
box 7-5 to ensure the pressure and temperature control inside the enrichment box 7-5. In
the present disclosure, the schematic diagram of the structure of the enrichment bin 7 is
shown in FIG. 5.
[0040] In the present disclosure, the dryer 9 is preferably a blast dryer or a fluidized
bed.
[0041] The present disclosure also provides a method for germinatingy-aminobutyric
acid-enriched highland barley based on the device described in the above solution, which
includes the following steps:
1) Fill the highland barley grains in the hopper 1;
2) Transfer the highland barley grains from the first conveying device 4 to the grain
cleaning machine 2, and start the grain cleaning machine to clean the highland barley
grains to obtain clean highland barley grains;
3) Transfer the clean highland barley grains to the steam sterilizer 3 by the first conveying
device 4, and start the steam sterilizer 3 to sterilize the clean highland barley grains to
obtain aseptic highland barley grains;
4) Transfer the aseptic highland barley grains to the germination tray 5-6 in the
germination cabinet 5 through the first conveying device 4, and carry out the first
germination and the second germination in sequence to obtain the highland barley
sprouts; the temperature of the first germination is 13~18'C, the time is 0.1~30h; the
temperature of the second germination is 28~32C, the time is 0.1~30h;
) Transfer the highland barley sprouts to the enrichment bin 7 through the second
conveying device 6, and after being evacuated by a vacuum peristaltic pump, they are
enriched at45-55C for 0.1-30 hours to obtain enriched germination;
6) Convey the enriched sprouts to the dryer 9 through the third conveying device 8 and
dry at 55-65 °C for 10-12 hours to obtain highland barley sprouts enriched in y
aminobutyric acid.
[0042] The process flow chart of the method for germination of highland barley with
y-aminobutyric acid enriched in the present disclosure is shown in FIG. 6.
[0043] The present disclosure first fills the highland barley grains in the feeding hopper; the highland barley grains are preferably selected highland barley grains with full grains, mold-free grains, no insects, and a germination rate greater than 90%; during the specific implementation of the present disclosure, in order to ensure consistent germination, the grain size is classified, and the grains of the same size are selected to germinate in the same batch; the present disclosure has no special restriction on the varieties of the highland barley, and preferably includes two-edge or six- edge.
[0044] In the present disclosure, the highland barley grains are transferred from the first conveying device 4 to the grain cleaning machine 2, and the grain cleaning machine is started to clean the highland barley grains to obtain clean highland barley grains; the cleaning method is preferably spraying the highland barley grains with clean water to wash away the floating dust on the surface of the grain; the spray cleaning time is preferably 30-60s, more preferably 40-50s; compressed air is introduced during the cleaning process to make the grains float to ensure sufficient cleaning.
[0045] After the clean highland barley grains are obtained, the present disclosure transfers the clean highland barley grains from the first conveying device 4 to the steam sterilizer 3, and starts the steam sterilizer 3 to sterilize the clean highland barley grains to obtain aseptic highland barley grains; the present disclosure uses hot steam to blanch and sterilize highland barley seeds, the thickness of the sterilized grain layer is preferably 0.5 to 1.0 cm, and the time is preferably 30 to 60 s, more preferably 40 to 50 s.
[0046] After obtaining the aseptic highland barley grains, the present disclosure transfers the aseptic highland barley grains to the germination tray 5-6 in the germination cabinet 5 through the first conveying device 4, and carry out the first germination and the second germination in sequence to obtain the highland barley sprouts; The temperature of the first germination is 13-18'C, preferably 15'C, and the time is 0.1-30h, preferably 5-20h; Before transfer the aseptic highland barley grains to the germination tray 5-6 of the germination cabinet 5 through the first transfer device 4 thegermination cabinet 5 is preferably sterilized; the temperature of the second germination is 28-32°C, preferably 30°C, and the time is 0.1-30h, preferably; The present disclosure uses gradient heating to germinate to provide a material basis for GABA enrichment by promoting protein degradation into free amino acids.
[0047] In the present disclosure, during the first germination, the thickness of the
highland barley grain layer is preferably 0.5 to 1.0 cm, more preferably 0.8 cm;
preferably spray humidification is performed every 3 to 5 hours, more preferably 4 hours;
The amount of water accounts for 8%-12% of the weight of the aseptic highland barley
grains, preferably 10%; the aeration interval is preferably 6-8h, more preferably 7h, and
each aeration time is preferably 10-15min, more preferably 12min.
[0048] In the present disclosure, during the second germination, the thickness of the
highland barley grain layer is preferably 0.5 to 1.0 cm, more preferably 0.8 cm; preferably,
spray humidification is performed every 5 to 7 hours, preferably 6 hours; the amount of
water added per time accounts for 5% to 8% of the weight of the aseptic highland barley
grains, preferably 6%; the aeration interval is preferably 3 to 4 hours, more preferably
3.5 hours, and each aeration time is preferably 10 to 15 minutes, more preferably 12
minutes.
[0049] After the highland barley sprouts are obtained, the present disclosure transfers
the highland barley sprouts to the enrichment bin 7 through the second conveying device
6. After being evacuated by a vacuum peristaltic pump, it is enriched at 45-55C for0.1
h to obtain enriched sprouts; the temperature of the enrichment germination is
preferably 50°C, and the time is preferably 1-20h, more preferably 5-10h; The filling
amount of the highland barley sprouts is preferably 3/4-4/5 of the volume of the
enrichment bin; during the specific implementation of the present disclosure, the volume
of the enrichment bin is subject to the volume of the enrichment tank; after being
evacuated by the vacuum peristaltic pump, the oxygen content in the enrichment tank is
%. The enrichment environment of the enrichment bin of the present disclosure is a
high temperature and low oxygen enrichment environment, and the glutamate
decarboxylase is activated through the stress of the high temperature and low oxygen
environment to realize GABA enrichment.
[0050] After the enrichment sprouts are obtained, the enrichment sprouts are
transmitted to the dryer 9 through the third conveying device 8, and dried at 55-65 °C
for 10-12h to obtain the y- aminobutyric acid enriched highland barley sprouts; The
drying temperature is preferably 60 °C and the drying time is preferably 11h.
[0051] The technical solutions of the present disclosure will be clearly and completely
described below with reference to the embodiments of the present disclosure. Obviously,
the described embodiments are only a part of the embodiments of the present disclosure,
rather than all the embodiments. Based on the embodiments of the present disclosure, all
other embodiments obtained by those of ordinary skill in the art without creative work
shall fall within the protection scope of the present disclosure.
[0052] In the embodiment of the present disclosure, the navy 2000 with a germination
rate greater than 90% is uniformly selected.
Example 1
[0053] (1) Grain screening: use color sorter and specific gravity machine to screen
highland barley grains to remove rotten grains, pest grains, immature grains and sand
and gravel;
[0054] (2) Grain cleaning: pass the selected highland barley grains through the feeding
hopper and evenly spread on the conveying device. Deliver to the grain cleaning machine
with the conveying device, and clean by pneumatic spray for 45s;
[0055] (3) Steam blanching sterilization: adjust the thickness of the highland barley delivered to the steam sterilizer to 1.0cm by adjusting the height of the screw rod of the grain layer thickness control ruler at the front of the steam sterilizer. Sterilize for 45s in a sterilizer filled with hot steam;
[0056] (4) Gradient heating germination: turn on the ultraviolet sterilization system of the germination cabinet in the germination chamber in advance to ensure the sterility of the germination environment, and adjust the temperature of the germination chamber to 16°C . The highland barley sterilized by steam blanching is evenly spread on the germination tray through the conveying device, with a thickness of1.0cm. After placing the germination tray in the germination cabinet, close the germination chamber, and begin to germinate with gradient heating.
[0057] Low temperature germination stage: germination temperature is 16°C, time is 16.0h, grain layer thickness is 1.0cm, spray humidification every 4.0h, water amount is % (w/w); ventilation interval is 7.0h, ventilation time is 15min;
[0058] Medium temperature germination stage: germination temperature is 30°C, time is 8.0h, grain layer thickness is 1.0cm, spray humidification every 6.0h, water amount is 6% (w/w); ventilation interval is 3.5h, ventilation time is 15min; The spray water is the water sterilized by ultraviolet in the water storage tank;
[0059] (5) High temperature and low oxygen enrichment: The germinated highland barley in the germination bin enters the enrichment bin through the conveying device. After the material filling volume reaches 3/4-4/5 of the volume of the enrichment bin, close the sealing cover and open the vacuum peristalsis pump to form a vacuum state in the enrichment bin. Turn on the steam system, heat the enrichment chamber to 50°C, and enrich for 6.Oh in a high temperature and low oxygen environment.
[0060] (6) Drying: Dry the GABA highland barley having completed the high temperature and low oxygen enrichment at 65C for 10 hours with a blast dryer to reduce the moisture of the GABA highland barley to below 12% of the safe moisture.
[0061] Determine the obtained product by liquid chromatography, and the GABA
content was 53.561.51mg/100g.
Example 2
[0062] (1) Grain screening: use color sorter and specific gravity machine to screen
highland barley grains to remove rotten grains, pest grains, immature grains and sand
and gravel;
[0063] (2) Grain cleaning: Pass the selected highland barley grains through the
feeding hopper and spread evenly on the conveying device. Deliver to the grain cleaning
machine with the conveying device, and clean by pneumatic spray for 45s to wash away
the floating dust on the surface of the grains;
[0064] (3) Steam blanching sterilization: adjust the thickness of the highland barley
delivered to the steam sterilizer to 1.0cm by adjusting the height of the screw rod of the
grain layer thickness control ruler at the front of the steam sterilizer. Sterilize in a
sterilizer full of hot steam for 45s to reduce the growth of bacteria;
[0065] (4) Low temperature germination: Turn on the UV sterilization system of the
germination cabinet in the germination chamber in advance to ensure the germination
environment is sterile, and adjust the germination chamber temperature to 16°C. The
highland barley sterilized by steam blanching is evenly spread on the germination tray
through the conveying device, with a thickness of1.0cm. After placing the germination
tray in the germination cabinet, close the germination chamber and start to germinate at
low temperature. Temperature is 16°C, time is 30.0h, grain layer thickness is 1.0cm,
spray humidification every 4.0h, water volume is 6% (w/w); ventilation interval is 7.0h, ventilation time is 15min;
[0066] (5) Drying: complete the low-temperature germination of the highland barley, and dry it with a blast dryer at 65C for 10 hours to reduce the GABA highland barley moisture to less than 12% of the safe moisture.
[0067] Determine the obtained product by liquid chromatography, and the GABA content was 28.740.29mg/100g.
Example 3
[0068] (1) Grain screening: use color sorter and specific gravity machine to screen highland barley grains to remove rotten grains, pest grains, immature grains and sand and gravel;
[0069] (2) Grain cleaning: pass the selected highland barley grains through the hopper, and evenly spread on the conveying device. Deliver to the grain cleaning machine with the conveying device, which is pneumatically sprayed for 45s to wash away the floating dust on the grain surface;
[0070] (3) Steam blanching sterilization: adjust the thickness of the highland barley delivered to the steam sterilizer to 1.0cm by adjusting the height of the screw rod of the grain layer thickness control ruler at the front of the steam sterilizer,. Sterilize in a sterilizer full of hot steam for 45s to reduce the growth of bacteria;
[0071] (4) Germination at medium temperature: Turn on the ultraviolet sterilization system of the germination cabinet in the germination chamber in advance to ensure the germination environment is sterile, and adjust the temperature of the germination chamber to 30°C. Spread the highland barley sterilized by steam blanching evenly on the germination tray through the conveying device, with a thickness of1.0cm. After placing the germination tray in the germination cabinet, close the germination chamber and start to germinate at low temperature. The temperature is 30'C, the time is 30.0h, the thickness of the grain layer is 1.0cm, and the humidification is sprayed every 4.0h, the amount of water is 6% (w/w); the ventilation interval is 7.0h, and the ventilation time is 15min;
[0072] (5) Drying: Dry the highland barley that has germinated and germinated at medium temperature for 10 hours with a blast dryer at 65C to reduce the moisture of the GABA highland barley to below 12% of the safe moisture.
[0073] Determine the obtained product by liquid chromatography, and the content of GABA was 32.571.33mg/100g.
Example 4
[0074] (1) Grain screening: use color sorter and specific gravity machine to screen highland barley grains to remove rotten grains, pest grains, immature grains and sand and gravel;
[0075] (2) Grain cleaning: Pass the selected highland barley grains through the hopper, and evenly spread on the conveying device. Deliver to the grain cleaning machine with the conveying device, t, which is pneumatically sprayed for 45s to wash away the floating dust on the grain surface;
[0076] (3) Steam blanching sterilization: adjust the thickness of the highland barley delivered to the steam sterilizer to 1.0cm by adjusting the height of the screw rod of the grain layer thickness control ruler at the front of the steam sterilizer. Sterilize in a sterilizer full of hot steam for 45s to reduce the growth of bacteria;
[0077] (4) High temperature and low oxygen enrichment: The high temperature sterilized by steam blanching enters the enrichment bin through the conveyor. After the material filling volume reaches 3/4-4/5 of the volume of the enrichment bin, close the sealing cover and open the vacuum peristaltic pump, to form a vacuum state in the enrichment bin. Turn on the steam system, heat the enrichment chamber to 50°C, and enrich for 30.Oh in a high temperature and low oxygen environment;
[0078] (5) Drying: complete the high temperature and low oxygen enrichment of
highland barley, and dry it with a blast dryer at 65C for 10 hours to reduce the GABA
highland barley moisture to below 12% of the safe moisture.
[0079] Determine the obtained product by liquid chromatography, and the GABA
content was 27.45±1.36 mg/100.
[0080] The above are merely the preferred embodiments of the present disclosure. It
should be pointed out that for those of ordinary skill in the art,1 improvements and
modifications can be made without departing from the principle of the present disclosure,
and these improvements and modifications are also It should be regarded as the
protection scope of the present disclosure.
Claims (5)
1. A device for germination of highland barley with y-aminobutyric acid enriched, which is sequentially connected with a feed hopper (1), a grain cleaning machine (2), a steam sterilizer (3), a germination cabinet (5), an enrichment bin (7) and a dryer (9);
The feed hopper (1), the grain cleaning machine (2), the steam sterilizer (3) and the germination cabinet (5) are connected by a first conveying device (4); the germination cabinet (5) and the enrichment bin (7) are connected through a second conveying device (6); the enrichment bin (7) and the dryer (9) are connected through a third conveying device (8).
2. The device according to claim 1, wherein the grain cleaning machine (2) comprises a water tank (2-1), an atomizing spray head (2-2), a cleaning chamber (2-3), a conveying track (2-6) and a air compressor (2-7);
The water inlet of the atomizing spray head (2-2) is connected to the water tank (2-1), and the atomizing spray head (2-2) is arranged on the top of the cleaning chamber (2-3);
A conveying track (2-6) is arranged inside the cleaning chamber (2-3) for conveying highland barley grains;
The bottom of the cleaning chamber (2-3) is respectively provided with a compressed air inlet (2-4) and a drain (2-5);
The air compressor (2-7) is connected to the compressed air inlet (2-4) to deliver compressed air to the cleaning chamber (2-3).
3. The device according to claim 1, wherein the steam sterilizer (3) comprises a steam generator (3-1), a sterilization box (3-3), a conveying track (3-4), a grain layer thickness control rule (3-5) and a steam condensate water recycle assembly (3-7);
The bottom of the sterilization box (3-3) is provided with a steam inlet (3-2); the steam generator (3-1) is connected to the steam inlet (3-2) through a pipe, and is connected to the sterilization box (3-2); Steam is introduced into the interior of the sterilization box (3-3); the bottom of the sterilization box (3-3) is also provided with a drain (3-8), and the drain (3-8) is connected to the steam generator (3-1) through a pipe; the steam condensate recycle assembly (3-7) communicates with the steam generator (3-1) through a pipeline;
The inside of the sterilization box (3-3) is provided with a conveying track (3-4) and a grain layer thickness control rule (3-5); the grain layer thickness control rule (3-5) is arranged on the conveying track (3-4) the entrance;
The upper wall of the sterilization box (3-3) is provided with an air-vent (3-6); the air
vent (3-6) is connected to the steam condensate recycle assembly (3-7) through a pipe;
wherein the germination cabinet comprises a plurality of vertically distributed
germination units (5-7); the germination cabinet 5 is arranged in a germination chamber
that includes an air conditioning system and a temperature and humidity sensor;
Each germination unit (5-7) is provided with several layers of liquid collection tray (5-2,
an atomizing spray system (5-1) and an ultraviolet sterilization system (5-3); the liquid
collection tray (5-2) is provided with a drainage system (5-5). The atomized spray system
(5-1) and the ultraviolet sterilization system (5-3) are fixed on the top of the germination
cabinet or the lower part of the liquid collection tray (5-2) of the germination unit (5-7)
on the upper layer;
In each germination unit (5-7), the space between the liquid collection pan (5-2), the
spray system and the ultraviolet sterilization system is provided with a germination tray
track (5-4); for placing the germination tray (5-6);
wherein, the enrichment bin (7) comprises a steam generator (7-1), a vacuum peristaltic
pump (7-2), a thermal insulation interlayer (7-3), a coil heating tube (7-4), an enrichment
box (7-5), and a pressure sensor (7-6), temperature sensor (7-7) and sealing cover (7
8);The thermal insulation interlayer (7-3) is fixed to the outer side wall of the enrichment
box (7-5); the thermal insulation interlayer is provided with a tube-coil heating tube (7
4); the tube-coil heating tube (7-4) and a steam generator (7-1) is connected by a pipe;
The inner side wall of the enrichment box (7-5) is fixed with a pressure sensor (7-6) and
a temperature sensor (7-7); the upper part of the enrichment box (7-5) is provided with a
sealing cover (7-8); The outer side wall of the enrichment box (7-5) is provided with an
observation window (7-9); the bottom of the enrichment box (7-5) is provided with a discharge port (7-10) and an air-vent 7-11);
The air-vent (7-11) and the vacuum peristaltic pump (7-2) are connected by a pipe.
4. A method for germination of highland barley with y-aminobutyric acid enriched based on the device of any one of claims 1 to 3, comprising the following steps:
1) Fill the highland barley grains in the feed hopper (1);
2) Transfer the highland barley grains from the first conveying device (4) to the grain cleaning machine (2), and start the grain cleaning machine to clean the highland barley grains to obtain clean highland barley grains;
3) Transfer the clean highland barley grains to the steam sterilizer (3) by the first conveying device (4), and start the steam sterilizer (3) to sterilize the clean highland barley grains to obtain aseptic highland barley grains;
4) Transfer the aseptic highland barley seeds to the germination tray (5-6) in the germination cabinet (5) through the first transfer device (4), carry out afirst germination and a second germination in sequence to obtain a highland barley sprouts; A temperature of the first germination is 13-18°C and a time is 0.1-30h; a temperature of the second
germination is 28-32C and a time is 0.1-30h;
) Transfer the highland barley sprouts to the enrichment bin (7) through the second conveying device (6), and after being evacuated by a vacuum peristaltic pump, they are enriched at45-55C for 0.1-30hto obtain enriched spouts;
6) Convey the enriched sprouts to the dryer (9) through the third conveying device (8), and dry at 55-65C for 10-12 hours to obtain highland barley sprouts enriched in y
aminobutyric acid.
5. The method according to claim 4, wherein, during the first germination in step 4), the thickness of the highland barley grain layer is 0.5-1.0 cm, and spray humidification every 3 to 5 hours, an amount of water added each time accounts for 8% 12% of the weight of aseptic highland barley grains, a ventilation interval is 6-8h, and each ventilation time is 10-15min; wherein, during the second germination in step 4), a thickness of the highland barley grain layer is 0.5-1.0 cm, and spray humidification every 5-7h, an amount of water added each time accounts for 5% to 8% of the weight of the aseptic highland barley grains, a ventilation interval is 3 to 4 hours, and each ventilation time is 10 to 15 minutes; wherein in step 5), a filling amount of the highland barley sprouts accounts for 3/4 to 4/5 of the volume of the enrichment bin.
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| JP2590423B2 (en) * | 1994-02-01 | 1997-03-12 | 農林水産省中国農業試験場長 | Food material enriched in γ-aminobutyric acid |
| JP3137615B2 (en) * | 1999-04-22 | 2001-02-26 | 株式会社 エムアンドエム | γ-Aminobutyric acid-enriched germinated brown rice and γ-aminobutyric acid-enriched germinated brown rice flour |
| CN1259854C (en) * | 2004-05-21 | 2006-06-21 | 浙江新安化工集团股份有限公司 | Preparation of rice containing gamma amino-butyric acid |
| CN101273769B (en) * | 2008-04-24 | 2011-08-17 | 江南大学 | Method for preparing germinated brown rice with high-content gamma-aminobutyric acid |
| CN102160627A (en) * | 2011-03-22 | 2011-08-24 | 西藏月王生物技术有限公司 | Highland barley with rich gamma-aminobutyric acid and preparation method thereof |
| CN102885303B (en) * | 2012-10-09 | 2014-04-02 | 西藏月王生物技术有限公司 | High-r-aminobutyric-acid-content highland-barley red yeast and preparation method thereof |
| CN106071854A (en) * | 2016-06-01 | 2016-11-09 | 中国科学院成都生物研究所 | The production method of the Semen avenae nudae Fructus Hordei Germinatus of Sync enrichment flavone and gamma aminobutyric acid |
| CN108576599A (en) * | 2018-05-08 | 2018-09-28 | 西华大学 | Production method rich in γ-aminobutyric acid cereal and its equipment |
| CN110089684B (en) * | 2019-04-10 | 2022-07-26 | 华南理工大学 | Production method of high-yield cured whole highland barley powder rich in gamma-aminobutyric acid |
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