AU2020103973A4

AU2020103973A4 - Novel specific long-acting bacterial attractant for lucilia sericata and preparation method thereof

Info

Publication number: AU2020103973A4
Application number: AU2020103973A
Authority: AU
Inventors: Zhendong HUANG; Qianqian Le; Qing Wan; Ruiling Zhang; Zhong Zhang
Original assignee: Shandong First Medical University and Shandong Academy of Medical Sciences
Current assignee: Shandong First Medical University and Shandong Academy of Medical Sciences
Priority date: 2020-12-09
Filing date: 2020-12-09
Publication date: 2021-02-18
Anticipated expiration: 2028-12-09

Abstract

Austracy The present invention discloses a novel specific long-acting bacterial attractant for Lucilia sericata, including the following components in parts by mass, based on 100 parts: 5-10 parts of intestinal bacterial matrix of Lucilia sericata, 1-5 parts of soybean meal, 1-5 parts of fish meal, 1.0-1.5 parts of agar powder, 0.5-1 part of potassium chloride, 1-3 parts of glycerin, 0.1-0.5 part of urea and the balance of water. The present invention also provides a preparation method of the novel specific long-acting bacterial attractant for Lucilia sericata. The preparation method is simple and low in cost of raw materials; and the prepared attractant is long-acting and insensitive to harsh climates such as rainfall and wind. The novel specific long-acting bacterial attractant for Lucilia sericata in the present invention not only can be used for the density monitoring of Lucilia sericata, but also can be used for the trap-killing of Lucilia sericata, and has good functional expansibility. 1 Drawings of Description .Bat Bait Bat Water Bacteria LB Fig. 1 SIKirebsimea ocyfta M LB E Wate Fig.2 1

Description

Austracy

The present invention discloses a novel specific long-acting bacterial attractant for Lucilia sericata, including the following components in parts by mass, based on 100 parts: 5-10 parts of intestinal bacterial matrix of Lucilia sericata, 1-5 parts of soybean meal, 1-5 parts of fish meal, 1.0-1.5 parts of agar powder, 0.5-1 part of potassium chloride, 1-3 parts of glycerin, 0.1-0.5 part of urea and the balance of water. The present invention also provides a preparation method of the novel specific long-acting bacterial attractant for Lucilia sericata. The preparation method is simple and low in cost of raw materials; and the prepared attractant is long-acting and insensitive to harsh climates such as rainfall and wind. The novel specific long-acting bacterial attractant for Lucilia sericata in the present invention not only can be used for the density monitoring of Lucilia sericata, but also can be used for the trap-killing of Lucilia sericata, and has good functional expansibility.

Drawings of Description

.Bat Bait Bat

Water Bacteria LB

Fig. 1

SIKirebsimea ocyfta M LB E Wate

Fig.2

Description

NOVEL SPECIFIC LONG-ACTING BACTERIAL ATTRACTANT FOR LUCILIA SERICATA AND PREPARATION METHOD THEREOF

Technical Field

The present invention relates to the technical field of control of flies, in particular to a novel specific long-acting bacterial attractant for Lucilia sericata and a preparation method thereof.

Background

Lucilia sericata (Diptera: Calliphoridae, Calliphorinae, Lucilia) is a worldwide medical vector insect. It can mechanically spread a variety of pathogenic microorganisms, thereby causing infectious diseases. Its larvae are necrophagous, and can oviposit in rancid wounds and boils of a human body or parasitize in human digestive tract, wounds, facial features and other parts, thereby causing human digestive tract myiasis, nasal myiasis, etc., and threatening human health. Lucilia sericata can also cause myiasis in livestock and poultry, thereby being harmful to the livestock industry. Meanwhile, Lucilia sericata is also a main pest of ham and salted fish products. It is necessary to dry the ham and salted fish in the open air during processing. In this process, the flies may easily oviposit in the ham and the salted fish to breed fly maggots. Therefore, the control of the flies has always been a problem that restricts the improvement of quality of the ham and the salted fish, wherein the most major fly is Lucilia sericata. From 2003 to 2004, some illegal traders illegally applied trichlorfon and dichlorvos to prevent flies and maggots when processing the ham and the salted fish; and scandals such as "poisonous ham" and "poisonous salted fish" were successively disclosed by the news media, and then were brought into public focus. At present, due to the lack of other effective control technologies, the control

Description

of Lucilia sericata mainly depends on chemical control. However, with the continuous promotion of chemical control, the abuse and unreasonable use of pesticides have caused that Lucilia sericatabecomes resistant to many insecticides, thereby resulting in a vicious circle of "growing in the number and the killing difficulty of Lucilia sericata with the increasing killing". Meanwhile, the unreasonable use of pesticides has brought many negative effects, such as food safety (excessive pesticide residues), natural enemy killing, and human and animal poisoning incidents. Therefore, it is an urgent technical problem for those skilled in the art to explore a novel attracting method of Lucilia sericata and provide a novel specific long-acting bacterial attractant for Lucilia sericata and a preparation method thereof.

Summary

In view of this, the present invention provides a novel specific long-acting bacterial attractant for Lucilia sericata and a preparation method thereof. The preparation method is simple and low in cost of raw materials; and the prepared attractant is long-acting and insensitive to harsh climates such as rainfall and wind. The novel specific long-acting bacterial attractant for Lucilia sericata in the present invention not only can be used for the density monitoring of Lucilia sericata, but also can be used for the trap-killing of Lucilia sericata, and has good functional expansibility. To achieve the above purposes, the following technical solution is adopted in the present invention: A novel specific long-acting bacterial attractant for Lucilia sericata includes the following components in parts by mass, based on 100 parts: 5-10 parts of intestinal bacterial matrix of Lucilia sericata, 1-5 parts of soybean meal, 1-5 parts of fish meal, 1.0-1.5 parts of agar powder, 0.5-1 part of potassium chloride, 1-3 parts of glycerin, 0.1-0.5 part of urea and the balance of water.

Description

The preferred technical solution has the beneficial effects that the novel specific long-acting bacterial attractant for Lucilia sericata disclosed by the present invention takes bacteria as an attractant, agar as a carrier, and sorbitol as a humectant, so that the attractant can keep the water content moderate for a long term and keep the physical properties of the attractant long-acting. Meanwhile, the attractant has nutrients and water conditions suitable for the growth of bacteria, so that the bacteria can continuously reproduce in the attractant to continuously attract the Lucilia sericata and thus keep the attracting effect long-acting. Compared with traditional attractants, the attractant of the present invention can keep valid for 1-2 months, and is insensitive to harsh climates such as rainfall and wind. Preferably, the intestinal bacterial matrix of Lucilia sericata is produced by culturing Klebsiella oxytoca (preservation unit: China General Microbiological Culture Collection Center (CGMCC); preservation address: Institute of Microbiology, Chinese Academy of Sciences (IMCAS), Beijing Institute of Microbiology, Chinese Academy of Sciences, No. 3, Courtyard 1, Beichen Road West, Chaoyang District, Beijing; preservation number: CGMCC No. 15976; preservation time: June 20, 2018; and classification and nomenclature: Klebsiella oxytoca) isolated from intestines of Lucilia sericata. Preferably, the preservation number of the Klebsiella oxytoca is CGMCC No. 15976. The preferred technical solution has the beneficial effect that the intestinal bacterial matrix of Lucilia sericataadopted in the present invention is produced by culturing Klebsiella oxytoca isolated from the intestines of Lucilia sericata, and has an effect of attracting the Lucilia sericata. The present invention also provides a preparation method of the novel specific long-acting bacterial attractant for Lucilia sericata, specifically including the following steps: (1) preparation of bacterial matrix: sequentially culturing and centrifuging bacteria isolated from intestines of Lucilia sericatato obtain the bacterial matrix;

Description

(2) weighing raw materials according to the novel specific long-acting bacterial attractant for Lucilia sericatafor later use; (3) preparation of bacterial growth medium: mixing the soybean meal, the fish meal, the agar powder, the potassium chloride, the glycerin, the urea and the water uniformly, and then autoclaving and cooling the mixture to prepare the bacterial growth medium; (4) preparation of Lucilia sericata attractant: adding the overnight cultured bacterial matrix to the bacterial growth medium and mixing uniformly the materials to obtain a gel; then pouring the gel into culture plates based on sterile operation in a clean bench; and preparing the Lucilia sericata attractant after the gel is cooled and solidified. The preferred technical solution has the beneficial effects that the preparation method disclosed by the present invention is simple; the bacteria isolated from the intestines of Lucilia sericata are cultured to obtain the bacterial matrix; and then the bacterial matrix is mixed with the bacterial growth medium to obtain the specific long-acting bacterial attractant. Preferably, the step (1) specifically includes: adding the bacteria isolated from the intestines of Lucilia sericata to an LB culture medium; then, shaking and culturing the bacteria at 37°C and 100 rpm to a logarithmic phase; taking out and adding the culture medium to a 10 ml centrifuge tube; centrifuging for 10 min; and collecting precipitates as the bacterial matrix. Preferably, the bacteria isolated from the intestines of Lucilia sericata include Klebsiella oxytoca. Preferably, the mixture is cooled to 55°C in the step (3). Preferably, the thickness of the gel after pouring the gel into the culture plates in the step (4) is 1-2 cm. It can be known from the above technical solutions that, compared with the prior art, the novel specific long-acting bacterial attractant for Lucilia sericata and the preparation method thereof provided by the present invention have the following beneficial effects:

Description

(1) A variety of factors are used to produce specific long-term attraction for the Lucilia sericata, wherein the bacteria are the major factors and supplemented with fish meal, which cooperate with each other to improve the attracting effect; (2) The agar is taken as the attractant carrier, and glycerin and urea are used as the humectant, to keep the water content of the attractant moderate for a long term and keep the physical properties of the attractant long-acting; (3) The attractant has nutrients and water conditions suitable for the growth of bacteria, so that the bacteria can continuously reproduce in the vector to continuously attract the Lucilia sericata and thus keep the attracting effect long-acting; (4) Compared with traditional attractants, the attractant of the present invention can keep valid for 1-2 months, and is insensitive to harsh climates such as rainfall and wind; (5) The novel specific long-acting bacterial attractant for Lucilia sericata of the present invention not only can be used for the density monitoring of Lucilia sericata, but also can be used for the trap-killing of Lucilia sericata, and has good functional expansibility; in addition, the specific attractant of the present invention is long-acting and low in cost, and is a specific long-acting attractant for Lucilia sericatawith broad application prospects.

Description of Drawings

To more clearly describe the technical solution in the embodiments of the present invention or in the prior art, the drawings required to be used in the description of the embodiments or the prior art will be simply presented below. Apparently, the drawings in the following description are merely the embodiments of the present invention, and for those ordinary skilled in the art, other drawings can also be obtained according to the provided drawings without contributing creative labor. Fig. 1 shows small fly trap cages provided by the present invention;

Description

Fig. 2 shows an attracting effect of Klebsiella oxytoca on adults of Lucilia sericataprovided by the present invention; Fig. 3 shows changes in the weight of an attractant placed in the field for different times provided by the present invention; Fig. 4 shows changes in the number of bacteria in the attractant placed in the field for different times provided by the present invention; and Fig. 5 shows ratios of flies (A) to Lucilia sericata (B) attracted by three attractants in different farms according to the present invention.

Detailed Description

The technical solution in the embodiments of the present invention will be clearly and fully described below in combination with the drawings in the embodiments of the present invention. Apparently, the described embodiments are merely part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments in the present invention, all other embodiments obtained by those ordinary skilled in the art without contributing creative labor will belong to the protection scope of the present invention. Embodiments of the present invention disclose a novel specific long-acting bacterial attractant for Lucilia sericata, including the following components in parts by mass, based on 100 parts: 5-10 parts of intestinal bacterial matrix of Lucilia sericata, 1-5 parts of soybean meal, 1-5 parts of fish meal, 1.0-1.5 parts of agar powder, 0.5-1 part of potassium chloride, 1-3 parts of glycerin, 0.1-0.5 part of urea and the balance of water. Embodiments of the present invention also disclose a preparation method of the novel specific long-acting bacterial attractant for Lucilia sericata, specifically including the following steps: (1) preparation of bacterial matrix: bacteria isolated from intestines of Lucilia sericatawere added to an LB culture medium, and then were shaken and cultured

Description

at 37°C and 100 rpm to a logarithmic phase; the culture medium was taken out and added to a 10 ml centrifuge tube to be centrifuged for 10 min; and precipitates were collected as the bacterial matrix; (2) raw materials were weighed according to the novel specific long-acting bacterial attractant for Lucilia sericatafor later use; (3) preparation of bacterial growth medium: the soybean meal, the fish meal, the agar powder, the potassium chloride, the glycerin, the urea and the water were mixed uniformly, and then autoclaved and cooled to 55°C to prepare the bacterial growth medium; (4) preparation of Lucilia sericata attractant: the overnight cultured bacterial matrix was added to the bacterial growth medium and mixed uniformly to obtain a gel; then the gel was poured into culture plates based on sterile operation in a clean bench so that the thickness of the gel is 1-2 cm; and the gel was prepared into the Lucilia sericataattractant after being cooled and solidified. To further optimize the technical solution, the bacteria isolated from the intestines of Lucilia sericata include Klebsiella oxytoca. Embodiment 1 Embodiment 1 of the present invention discloses a novel specific long-acting bacterial attractant for Lucilia sericata, including the following components based on 100 g: 5 g of intestinal bacterial matrix of Lucilia sericata,1 g of soybean meal, 1 g of fish meal, 1.0 g of agar powder, 0.5 g of potassium chloride, 1 g of glycerin, 0.1 g of urea and the balance of water. Embodiment 1 of the present invention also discloses a preparation method of the novel specific long-acting bacterial attractant for Lucilia sericata, specifically including the following steps: (1) preparation of bacterial matrix: Klebsiella oxytoca isolated from intestines of Lucilia sericatawas added to an LB culture medium, and then was shaken and cultured at 37°C and 100 rpm to a logarithmic phase; the culture medium was

Description

taken out and added to a 10 ml centrifuge tube to be centrifuged for 10 min; and precipitates were collected as the bacterial matrix; (2) raw materials were weighed according to the novel specific long-acting bacterial attractant for Lucilia sericatafor later use; (3) preparation of bacterial growth medium: the soybean meal, the fish meal, the agar powder, the potassium chloride, the glycerin, the urea and the water were mixed uniformly, and then autoclaved and cooled to 55°C to prepare the bacterial growth medium; (4) preparation of Lucilia sericata attractant: the overnight cultured bacterial matrix was added to the bacterial growth medium and mixed uniformly to obtain a gel; then the gel was poured into culture plates based on sterile operation in a clean bench so that the thickness of the gel is 1-2 cm; and the gel was prepared into the Lucilia sericataattractant after being cooled and solidified. Embodiment 2 Embodiment 2 of the present invention discloses a novel specific long-acting bacterial attractant for Lucilia sericata, including the following components based on 100 g: 10 g of intestinal bacterial matrix of Lucilia sericata, 5 g of soybean meal, 5 g of fish meal, 1.5 g of agar powder, 1 g of potassium chloride, 3 g of glycerin, 0.5 g of urea and the balance of water. Embodiment 2 of the present invention also discloses a preparation method of the novel specific long-acting bacterial attractant for Lucilia sericata, specifically including the following steps: (1) preparation of bacterial matrix: bacteria isolated from intestines of Lucilia sericatawere added to an LB culture medium, and then were shaken and cultured at 37°C and 100 rpm to a logarithmic phase; the culture medium was taken out and added to a 10 ml centrifuge tube to be centrifuged for 10 min; and precipitates were collected as the bacterial matrix;

Description

(2) raw materials were weighed according to the novel specific long-acting bacterial attractant for Lucilia sericatafor later use; (3) preparation of bacterial growth medium: the soybean meal, the fish meal, agar powder, potassium chloride, glycerin, urea and water were mixed uniformly, and then autoclaved and cooled to 55°C to prepare the bacterial growth medium; (4) preparation of Lucilia sericata attractant: the overnight cultured bacterial matrix was added to the bacterial growth medium and mixed uniformly to obtain a gel; then the gel was poured into culture plates based on sterile operation in a clean bench so that the thickness of the gel is 1-2 cm; and the gel was prepared into the Lucilia sericataattractant after being cooled and solidified. Embodiment 3 Embodiment 3 of the present invention discloses a novel specific long-acting bacterial attractant for Lucilia sericata, including the following components based on 100 g: 7 g of intestinal bacterial matrix of Lucilia sericata,3 g of soybean meal, 3 g of fish meal, 1.3 g of agar powder, 0.7 g of potassium chloride, 2 g of glycerin, 0.4 g of urea and the balance of water. The present embodiment of the present invention also discloses a preparation method of the novel specific long-acting bacterial attractant for Lucilia sericata, specifically including the following steps: (1) preparation of bacterial matrix: Klebsiella oxytoca isolated from intestines of Lucilia sericatawas added to an LB culture medium, and then was shaken and cultured at 37°C and 100 rpm to a logarithmic phase; the culture medium was taken out and added to a 10 ml centrifuge tube to be centrifuged for 10 min; and precipitates were collected as the bacterial matrix; (2) raw materials were weighed according to the novel specific long-acting bacterial attractant for Lucilia sericatafor later use; (3) preparation of bacterial growth medium: the soybean meal, the fish meal, the agar powder, the potassium chloride, the glycerin, the urea and the water were

Description

mixed uniformly, and then autoclaved and cooled to 55°C to prepare the bacterial growth medium; (4) preparation of Lucilia sericata attractant: the overnight cultured bacterial matrix was added to the bacterial growth medium and mixed uniformly to obtain a gel; then the gel was poured into culture plates based on sterile operation in a clean bench so that the thickness of the gel is 1-2 cm; and the gel was prepared into the Lucilia sericataattractant after being cooled and solidified. Embodiment 4 Embodiment 4 of the present invention discloses a novel specific long-acting bacterial attractant for Lucilia sericata, including the following components based on 100 g: 9 g of intestinal bacterial matrix of Lucilia sericata,4 g of soybean meal, 4 g of fish meal, 1.2 g of agar powder, 0.6 g of potassium chloride, 2.5 g of glycerin, 0.4 g of urea and the balance of water. Embodiment 4 of the present invention also discloses a preparation method of the novel specific long-acting bacterial attractant for Lucilia sericata, specifically including the following steps: (1) preparation of bacterial matrix: Klebsiella oxytoca isolated from intestines of Lucilia sericatawas added to an LB culture medium, and then was shaken and cultured at 37°C and 100 rpm to a logarithmic phase; the culture medium was taken out and added to a 10 ml centrifuge tube to be centrifuged for 10 min; and precipitates were collected as the bacterial matrix; (2) raw materials were weighed according to the novel specific long-acting bacterial attractant for Lucilia sericatafor later use; (3) preparation of bacterial growth medium: the soybean meal, the fish meal, the agar powder, the potassium chloride, the glycerin, the urea and the water were mixed uniformly, and then autoclaved and cooled to 55°C to prepare the bacterial growth medium; (4) preparation of Lucilia sericata attractant: the overnight cultured bacterial matrix was added to the bacterial growth medium and mixed uniformly to obtain a gel; then the gel was poured into culture plates based on sterile operation in a clean

Description

bench so that the thickness of the gel is 1-2 cm; and the gel was prepared into the Lucilia sericataattractant after being cooled and solidified. 1. Attracting effect of bacteria on adults of Lucilia sericatain laboratory Designed small fly trap cages as shown in Fig. 1 had a length, width and height of 10 cm. Meanwhile, a funnel-like attracting opening was designed at the top and connected with the fly trap cage; and a circular opening was designed at the top of the funnel for the Lucilia sericata to enter. The whole fly trap cages were covered with meshes; the top opening was open; but hard nylon wires were placed inside the openings to prevent the entering Lucilia sericatafrom flying out (Fig. 1). Different baits were placed in the fly trap cages. The baits in this experiment included the isolated bacteria, LB culture solution and water, which were respectively mixed with wheat bran so that the water content was 40-50%. The small fly trap cages containing three baits were placed in an insect cage with a length, width and height of 40 cm; and the insect cage was covered with the mesh. Lucilia sericatawere placed in the insect cage. The number of Lucilia sericata in each small fly trap cage was counted after 4 h. Three groups of experiments were repeated. Results as shown in Fig. 4 indicated that only the Klebsiella oxytoca isolated from the intestines of Lucilia sericata had a significant attracting effect on the adults of Lucilia sericata. Compared with LB and water, Klebsiella oxytoca could attract 77.22+3.51% of the adults of Lucilia sericata. 2. Changes in the number of bacteria in the attractant and the weight of vector placed in the field for different times 1 g of attractant was separately taken and ground in 50 ml of sterile water; then 0.5 ml of attractant was taken for coating test; and the number of bacterial colonies on a flat plate was counted after 12 h. 100g of attractant was separately placed in culture dishes; the attractants were placed in the field for different times to count the bacterial colonies; the attractants were respectively placed in a direct sunlight environment, a shade environment and a simulated rainfall environment (water was added to the external environment for soaking) for 3 d, 7 d, 10 d, 14 d

Description

and 21 d, and then were brought back to the laboratory for counting the bacterial colonies after weighing. The results showed that after 42 d, the weight of the attractant decreased by about 20 g and the weight loss rate was about 20% in the absence of precipitation; and the weight of the attractant decreased by about 10 g and the weight loss rate was about 10% in the presence of precipitation (Fig. 3). The above experiments show that the attractant of the present invention has good water holding capacity in the field, can keep the physical properties for a long term, and is beneficial to play a role of attraction. Meanwhile, the checked number of bacteria in the attractant shows that the number of bacteria in the attractant placed in the field can be increased to 6 times of the initial number, and particularly in the rainfall environment, the growth efficiency is higher, up to 8.78x108c fu/g, while the original number of bacteria in the attractant is only 1.25x108c fu/g, thereby ensuring that the attractant can maintain the attracting effect for a long term (Fig. 4). Field application example (1) of embodiment 1: Putrid fish viscera, sweet and sour liquid (25 g of brown sugar + 25 g of aged vinegar + 25 ml of water) and the bacterial attractant prepared in embodiment 1 of the present invention were used as baits respectively, for trapping Lucilia sericata in the field with a cage trapping method. Five monitoring sites, including a farmer's market, a waste transfer station, a farm, a green belt and a residential area, were respectively selected as trapping sites. Three fly trap cages with specifications specified by national standards were placed at each monitoring site; three baits were respectively placed in the fly trap cages; and a total of 15 fly trap cages were deployed. The fly trap cages were placed at 9:00-10: 00 in the morning and taken back at 15: 00-16: 00 in the afternoon. The adult flies were killed with ethyl acetate and then classified and counted. The results showed that the number of flies attracted by the bacterial attractant of the present invention was largest in flies attracted by different attractants in three farms, accounting for nearly 49% of the total number of attracted flies. The classification statistic for the attracted flies

Description

showed that the number of Lucilia sericata attracted by the bacterial attractant accounted for nearly 61% of the total number of the attracted Lucilia sericata, which further indicates that the attractant of the present invention has stronger attracting specificity to the adults of Lucilia sericata (Fig. 5). Each embodiment in the description is described in a progressive way. The difference of each embodiment from each other is the focus of explanation. The same and similar parts among all of the embodiments can be referred to each other. For the device disclosed by the embodiments, because the device corresponds to a method disclosed by the embodiments, the device is simply described. Refer to the description of the method part for the related part. The above description of the disclosed embodiments enables those skilled in the art to realize or use the present invention. Many modifications to these embodiments will be apparent to those skilled in the art. The general principle defined herein can be realized in other embodiments without departing from the spirit or scope of the present invention. Therefore, the present invention will not be limited to these embodiments shown herein, but will conform to the widest scope consistent with the principle and novel features disclosed herein.

Claims

1. A novel specific long-acting bacterial attractant for Lucilia sericata, comprising the following components in parts by mass, based on 100 parts: 5-10 parts of intestinal bacterial matrix of Lucilia sericata, 1-5 parts of soybean meal, 1-5 parts of fish meal, 1.0-1.5 parts of agar powder, 0.5-1 part of potassium chloride, 1-3 parts of glycerin, 0.1-0.5 part of urea and the balance of water.

2. The novel specific long-acting bacterial attractant for Lucilia sericata according to claim 1, wherein the intestinal bacterial matrix of Lucilia sericata is produced by culturing Klebsiella oxytoca isolated from intestines of Lucilia sericata.

3. A preparation method of the novel specific long-acting bacterial attractant for Lucilia sericata, specifically comprising the following steps: (1) preparation of bacterial matrix: sequentially culturing and centrifuging bacteria isolated from intestines of Lucilia sericatato obtain the bacterial matrix; (2) weighing raw materials according to the novel specific long-acting bacterial attractant for Lucilia sericataof claim 1 or 2 for later use; (3) preparation of bacterial growth medium: mixing the soybean meal, the fish meal, the agar powder, the potassium chloride, the glycerin, the urea and the water uniformly, and then autoclaving and cooling the mixture to prepare the bacterial growth medium; (4) preparation of Lucilia sericata attractant: adding the bacterial matrix cultured in step (1) to the bacterial growth medium and mixing uniformly the materials to obtain a gel; then pouring the gel into culture plates based on sterile operation in a clean bench; and preparing the Lucilia sericata attractant after the gel is cooled and solidified, wherein the thickness of the gel after pouring the gel into the culture plates is 1-2 cm.

4. The preparation method of the novel specific long-acting bacterial attractant for Lucilia sericata according to claim 3, wherein the step (1) specifically comprises: adding the bacteria isolated from the intestines of Lucilia sericata to an LB culture medium; then, shaking and culturing the bacteria at 37°C and 100 rpm to a

Claims

logarithmic phase; taking out and adding the culture medium to a 10 ml centrifuge tube; centrifuging for 10 min; and collecting precipitates as the bacterial matrix.

5. The preparation method of the novel specific long-acting bacterial attractant for Lucilia sericata according to claim 4, wherein the bacteria isolated from the intestines of Lucilia sericatacomprise Klebsiella oxytoca.