AU2020102307A4 - Compound plant stress resistance agent and preparation method and applications thereof - Google Patents
Compound plant stress resistance agent and preparation method and applications thereof Download PDFInfo
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N65/00—Biocides, pest repellants or attractants, or plant growth regulators containing material from algae, lichens, bryophyta, multi-cellular fungi or plants, or extracts thereof
- A01N65/03—Algae
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N33/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic nitrogen compounds
- A01N33/02—Amines; Quaternary ammonium compounds
- A01N33/12—Quaternary ammonium compounds
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N33/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic nitrogen compounds
- A01N33/16—Biocides, pest repellants or attractants, or plant growth regulators containing organic nitrogen compounds containing nitrogen-to-oxygen bonds
- A01N33/18—Nitro compounds
- A01N33/20—Nitro compounds containing oxygen or sulfur attached to the carbon skeleton containing the nitro group
- A01N33/22—Nitro compounds containing oxygen or sulfur attached to the carbon skeleton containing the nitro group having at least one oxygen or sulfur atom and at least one nitro group directly attached to the same aromatic ring system
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N43/00—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
- A01N43/02—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms
- A01N43/04—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom
- A01N43/06—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom five-membered rings
- A01N43/08—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom five-membered rings with oxygen as the ring hetero atom
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N43/00—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
- A01N43/02—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms
- A01N43/04—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom
- A01N43/14—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom six-membered rings
- A01N43/16—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom six-membered rings with oxygen as the ring hetero atom
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N47/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom not being member of a ring and having no bond to a carbon or hydrogen atom, e.g. derivatives of carbonic acid
- A01N47/08—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom not being member of a ring and having no bond to a carbon or hydrogen atom, e.g. derivatives of carbonic acid the carbon atom having one or more single bonds to nitrogen atoms
- A01N47/28—Ureas or thioureas containing the groups >N—CO—N< or >N—CS—N<
- A01N47/38—Ureas or thioureas containing the groups >N—CO—N< or >N—CS—N< containing the group >N—CO—N< where at least one nitrogen atom is part of a heterocyclic ring; Thio analogues thereof
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N55/00—Biocides, pest repellants or attractants, or plant growth regulators, containing organic compounds containing elements other than carbon, hydrogen, halogen, oxygen, nitrogen and sulfur
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N59/00—Biocides, pest repellants or attractants, or plant growth regulators containing elements or inorganic compounds
- A01N59/16—Heavy metals; Compounds thereof
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N59/00—Biocides, pest repellants or attractants, or plant growth regulators containing elements or inorganic compounds
- A01N59/16—Heavy metals; Compounds thereof
- A01N59/20—Copper
Abstract
Austracy
The present invention provides a compound plant stress resistance agent and a
preparation method and applications thereof and belongs to the technical field of
plant nutrition. The compound plant stress resistance agent includes the following
components in parts by weight: 0.4-0.8 part of organic germanium, 1-3 parts of
ascorbic acid, 0.5-3 parts of compound sodium nitrophenolate, 0.2-1 part of choline
chloride, 1-2 parts of allantoin, 5-10 parts of chitin, 3-6 parts of seaweed extract,
0.5-1 part of trace elements and 10-20 parts of potassium fulvic acid. The trace
elements include copper, manganese, zinc and iron according to a mass ratio of
(0.2-1.5):(2-4):(7-14):(15-25). Through synergistic effects of various components of
the compound plant stress resistance agent, self-protective mechanisms of plants
growing in an adverse environment are started and enhanced; tolerance of the plants
to the adverse environment is increased; and damage to the adverse environment is
decreased, thereby increasing stress resistance of the plants. The compound plant
stress resistance agent can obviously increase yield of multiple crops growing in the
adverse environment, particularly a salinized environment.
1
Description
Austracy
The present invention provides a compound plant stress resistance agent and a preparation method and applications thereof and belongs to the technical field of plant nutrition. The compound plant stress resistance agent includes the following components in parts by weight: 0.4-0.8 part of organic germanium, 1-3 parts of ascorbic acid, 0.5-3 parts of compound sodium nitrophenolate, 0.2-1 part of choline chloride, 1-2 parts of allantoin, 5-10 parts of chitin, 3-6 parts of seaweed extract, 0.5-1 part of trace elements and 10-20 parts of potassium fulvic acid. The trace elements include copper, manganese, zincand iron according to a mass ratio of (0.2-1.5):(2-4):(7-14):(15-25). Through synergistic effects of various components of the compound plant stress resistance agent, self-protective mechanisms of plants growing in an adverse environment are started and enhanced; tolerance of the plants to the adverse environment is increased; and damage to the adverse environment is decreased, thereby increasing stress resistance of the plants. The compound plant stress resistance agent can obviously increase yield of multiple crops growing in the adverse environment, particularly a salinized environment.
Description
Technical Field
The present invention belongs to the technical field of plant nutrition, and particularly relates to a compound plant stress resistance agent and a preparation method and applications thereof.
Background
Stress resistance mechanisms will be automatically started when plants grow in adverse environments, so as to avoid influences of external environments on normal plant growth. The adverse environments include drought resistance, saline-alkali tolerance, water-logging resistance, wind resistance, freezing resistance and resistance to insect pests. Under natural conditions, because of different geographical locations and weather conditions, human activities and other causes, various adverse environments go beyond a tolerable scope of normal growth and development of the plants. Thus, the plants are suffered from irreversible damage or even die. To increase stress resistance of the plants, common methods are as follows: a nutritional agent capable of increasing stress resistance of plants is applied; and stress resistance genes and resistance varieties are introduced for screening and breeding by a genetic engineering method. The genetic engineering method for introducing the stress resistance genes is a feasible method and is fast in stress resistance efficacy. However, preliminary screening of the stress resistance genes is a precondition of constructing transgene. During screening and breeding of the resistance varieties, the obtained resistance varieties of plants are often screened for a long time and have stable stress resistance, while due to long screening and breeding time, an immediate effect cannot be achieved. The method for applying
Description
the nutritional agent capable of increasing stress resistance of plants is fast in efficacy. The method may achieve the effect when the nutritional agent is applied in the same year generally, just like application of a potassium fertilizer. Potassium is the most influential element to plant health. Since the potassium participates in almost all of biophysical and biochemical processes in plant growth and development, the plants may generally have high stress resistance under stress conditions if the potassium nutrient element is abundantly supplied. The potassium plays an important role in aspects of enhancing cold resistance, drought resistance, saline-alkali tolerance, resistant to insect pests of the plants. As requirements for stress resistance of the plants are gradually improved, a stress resistance agent capable of achieving two or more stress resistance effects is expected to be added, and thus a compound stress resistance agent emerges. For example, a plant stress resistance agent disclosed by a patent with authorized announcement number of CN1O1142925B is composed of plant trace elements and multiple amino acids, and may achieve effects of increasing yield and resisting diseases. A stress-resistant vegetable yield-increasing agent disclosed by a patent with authorized announcement number of CN100355344B is composed of plant hormones and multiple plant nutrient components, and achieves effects of avoiding nutrition imbalance caused by single hormone action, increasing stress resistance of vegetables and facilitating fruit setting and fruit development of vegetables. A patent having publication number of CN1568707A discloses a stress-resistant poplar health agent. The stress-resistant poplar health agent is composed of mycorrhiza and plant hormones and can achieve effects of providing poplar productivity and increasing resistance of the poplar to diseases. The above compound stress resistance agents can increase the plant stress resistance in different degrees and further increase yield or growth vigor of the crops. However, the compound stress resistance agents mainly increase the stress resistance to insect pests, rather than stress resistance to salinization.
Description
Summary In view of this, a purpose of the present invention is to provide a compound plant stress resistance agent and a preparation method and applications thereof. The compound plant stress resistance agent can greatly increase plant activity and may enable plants growing in a salinization-resistant environment to have high resistance. To achieve the above purpose, technical solutions of the present invention are as follows: The compound plant stress resistance agent provided by the present invention includes the following components in parts by weight: 0.4-0.8 part of organic germanium, 1-3 parts of ascorbic acid, 0.5-3 parts of compound sodium nitrophenolate, 0.2-1 part of choline chloride, 1-2 parts of allantoin, 5-10 parts of chitin, 3-6 parts of seaweed extract, 0.5-1 part of trace elements and 10-20 parts of potassium fulvic acid. The trace elements include copper, manganese, zinc and iron; and a mass ratio of the copper to manganese to zinc to iron is (0.2-1.5):(2-4):(7-14):(15-25). Preferably, the compound plant stress resistance agent includes the following components in parts by weight: 0.5-0.7 part of organic germanium, 1.5-2.5 parts of ascorbic acid, 1-2.5 parts of compound sodium nitrophenolate, 0.4-0.8 part of choline chloride, 1.2-1.7 parts of allantoin, 6-8 parts of chitin, 4-5 parts of seaweed extract, 0.6-0.8 part of trace elements and 12-18 parts of potassium fulvic acid. The trace elements include copper, manganese, zinc and iron; and a mass ratio of the copper to manganese to zinc to iron is (0.5-1.2):(2.5-3.5):(8-12):(18-22). Preferably, the organic germanium is carboxylethyl germanium sesquioxide; a molecular formula of the carboxylethyl germanium sesquioxide is 6C HioGe 2O 7 ; and purity of the carboxylethyl germanium sesquioxide is higher than 99%. Preferably, the chitin is water-soluble chitin.
Description
Preferably, the copper is added in the form of amino acid chelated copper; the manganese is added in the form of manganese sulfate; the zinc is added in the form of zinc sulfate; and the iron is added in the form of amino acid chelated iron. Preferably, the ascorbic acid includes a stabilizer; and content of the stabilizer is 10-20% of the mass of the ascorbic acid. The present invention provides a preparation method of the compound plant stress resistance agent, including the following steps: (1) mixing manganese and zinc in trace elements with chitin; dissolving the obtained mixture and water according to a mass ratio of 1:(1-2); mixing the obtained mixed wet material with copper and iron in the trace elements and potassium fulvic acid in sequence; and drying the mixture so as to obtain a first mixture; (2) mixing organic germanium, compound sodium nitrophenolate, choline chloride, allantoin and seaweed extract so as to obtain a second mixture; (3) mixing the first mixture in the step (1), the second mixture in the step (2) and ascorbic acid, thereby obtaining the compound plant stress resistance agent. The present invention provides an application of the compound plant stress resistance agent or a compound plant stress resistance agent prepared by the preparation method in plant stress resistance. Preferably, the plant stress resistance includes salinization resistance. Preferably, a dilution ratio of the compound plant stress resistance agent is 1:(1000-1500); application modes of the compound plant stress resistance agent include root irrigation and foliage spray; a dosage of the compound plant stress resistance agent is 80-120 g per mu during root irrigation; and the dosage of the compound plant stress resistance agent is 40-60 g per mu during foliage spray. According to the compound plant stress resistance agent provided by the present invention, the organic germanium can obviously increase content and activity of protective enzymes, inhibit production of free radicals and lipid
Description
peroxidation of cytomembranes, and further prevent the cytomembranes from being damaged and preventing enzymes, proteins, nucleic acids and the like from being suffered from oxidative denaturation and maintaining activity; the ascorbic acid can effectively alleviate damage of reactive oxygen produced by plants in the adverse environments to plants, increase content of antioxidant enzymes in plant cells and enhance stress resistance of the plants under adversity stress; under the effects of the organic germanium and the ascorbic acid, the compound sodium nitrophenolate has effects of promoting protoplasmic streaming of the plant cells, increasing cell viability and increasing the stress resistance level of the plants; the chitin can increase antibacterial and antiviral abilities of the plants, regulate the immune system and increase adverse environment resistance of the plants; the seaweed extract can promote plant root development, promote water and nutrient absorption of the plants, increase activity of superoxide dismutase and other enzymes in the plants, enhance metabolic activities of the plants and increase plant stress resistance; the choline chloride can increase photosynthetic efficiency of the plants; the allantoin is beneficial for stimulating plant growth and increasing the influences of the plants for resisting adverse environments; and the trace elements are beneficial for supplementing trace elements essential for cellular response and increasing the cell viability and provide nutrient elements for plant growth. According to the compound plant stress resistance agent provided by the present invention, due to the synergistic effects of the trace elements, the chitin, the potassium fulvic acid, the organic germanium, the compound sodium nitrophenolate, the choline chloride, the allantoin and the seaweed extract, the self-protective mechanisms of the plants growing in the adverse environment are started and enhanced, the tolerance of the plants to the adverse environment is increased, and damage to the adverse environment is decreased, thereby increasing the plant activity. Experimental results prove that, compared with contrast cases, the compound stress resistance agent provided by the present invention
Description
significantly increases the yield of multiple crops growing in the salinized environment. Specifically, compared with that in the contrast cases, the yield of wheat, maize, cotton, peanut, sweet potato, pepper and cucumber is averagely increasedby 10.4%,7.1%,9.5%,11.9%,9.3%, 11.8% and 12.1% respectively; and compared with clear water, the yield of the wheat, maize, cotton, peanut, sweet potato, pepper and cucumber is increased by 22.6%, 25.3%, 28.3%, 25.2%, 14.9%, 35.2% and 32.8% respectively.
Detailed Description
The present invention provides a compound plant stress resistance agent, including the following components in parts by weight: 0.4-0.8 part of organic germanium, 1-3 parts of ascorbic acid, 0.5-3 parts of compound sodium nitrophenolate, 0.2-1 part of choline chloride, 1-2 parts of allantoin, 5-10 parts of chitin, 3-6 parts of seaweed extract, 0.5-1 part of trace elements and 10-20 parts of potassium fulvic acid. The trace elements include copper, manganese, zinc and iron; and a mass ratio of the copper to manganese to zinc to iron is (0.2-1.5):(2-4):(7-14):(15-25). The compound plant stress resistance agent provided by the present invention includes the organic germanium. The organic germanium is 0.4-0.8 part by weight, preferably 0.5-0.7 part, most preferably 0.6 part; the organic germanium is carboxylethyl germanium sesquioxide; a molecular formula of the carboxylethyl germanium sesquioxideis C6 HioGe 2 7 ; and purity of the carboxylethyl germanium
sesquioxide is higher than 99%. The source of the organic germanium is not specially limited in the present invention, and a source of the organic germanium well-known to those skilled in the art is available. In embodiments of the present invention, the organic germanium is purchased from Kramer Company. The organic germanium can obviously increase content and activity of protective enzymes, inhibit production of free radicals and lipid peroxidation of
Description
cytomembranes, and further prevent the cytomembranes from being damaged and preventing enzymes, proteins, nucleic acids and the like from being suffered from oxidative denaturation and maintaining activity. The compound plant stress resistance agent provided by the present invention includes the ascorbic acid. The ascorbic acid is 1-3 parts by weight, preferably 1.5-2.5 parts, most preferably 2 parts. The ascorbic acid preferably contains a stabilizer; and the stabilizer facilitates preventing oxidation of the ascorbic acid. The content of the stabilizer is preferably 10-20% of the mass of the ascorbic acid, preferably 15%. The stabilizer is preferably selected from one or more of potassium citrate, potassium sulfate and monopotassium phosphate. When one stabilizer is used, it is preferably sodium citrate; when two stabilizers are used, they are preferably potassium citrate and potassium sulfate, and a mass ratio of the potassium citrate to potassium sulfate is preferably 1:1; when three stabilizers are used, they are preferably potassium citrate, potassium sulfate and monopotassium phosphate; and a mass ratio of the potassium citrate to potassium sulfate to monopotassium phosphate is 2:2:1. The ascorbic acid can effectively alleviate damage of reactive oxygen produced by plants in an adverse environment to plants, increase content of antioxidant enzymes in plant cells and enhance stress resistance of the plants under adversity stress. Sources of the ascorbic acid and the stabilizer are not specially limited in the present invention, and source of the ascorbic acid and the stabilizer well-known to those skilled in the art are available. In embodiments of the present invention, the ascorbic acid is purchased from Zhengzhou Futai Chemical Co. Ltd. The compound plant stress resistance agent provided by the present invention includes the compound sodium nitrophenolate. The compound sodium nitrophenolate is 0.5-3 parts by weight, preferably 1-2.5 parts, most preferably 1.8 parts. The compound sodium nitrophenolate has effects of promoting protoplasmic streaming of the plant cells, increasing cell viability and increasing the stress
'7
Description
resistance level of the plants. The source of the compound sodium nitrophenolate is not specially limited in the present invention, and a source of the compound sodium nitrophenolate well-known to those skilled in the art is available. In embodiments of the present invention, the compound sodium nitrophenolate is purchased from Wuhan Yuancheng Gongchuang Science and Technology Co. Ltd. The compound plant stress resistance agent provided by the present invention includes the choline chloride. The choline chloride is 0.2-1 part by weight, preferably 0.4-0.8 part, most preferably 0.6 part. The choline chloride has an effect of increasing photosynthetic efficiency of the plants. The source of the choline chloride is not specially limited in the present invention, and a source of the choline chloride well-known to those skilled in the art is available. In embodiments of the present invention, the choline chloride is purchased from Jinan Jinhui Chemical Co., Ltd. The compound plant stress resistance agent provided by the present invention includes the allantoin. The allantoin is 1-2 parts by weight, preferably 1.2-1.7 parts, most preferably 1.5 parts. The allantoin is beneficial for stimulating plant growth and increasing the influences of the plants for resisting adverse environments. The source of the allantoin is not specially limited in the present invention, and a source of the allantoin well-known to those skilled in the art is available. In embodiments of the present invention, the allantoin is purchased from Wuhu Pusheng Pharmaceutical Co., Ltd. The compound plant stress resistance agent provided by the present invention includes the chitin. The chitin is 5-10 parts by weight, preferably 6-8 parts, most preferably 7 parts. The chitin is preferably water-soluble chitin. The chitin has effects of increasing antibacterial and antiviral abilities of the plants, regulating the immune system and increasing adverse environment resistance of the plants. The source of the chitin is not specially limited in the present invention, and a source of the chitin well-known to those skilled in the art is available. In embodiments of the
Description
present invention, the chitin is purchased from Zhengzhou Chaofan Chemical Co., Ltd. The compound plant stress resistance agent provided by the present invention includes the seaweed extract. The seaweed extract is 3-6 parts, preferably 4-5 parts, most preferably 4.5 parts. The seaweed extract can promote plant root development, promote water and nutrient absorption of the plants, increase activity of superoxide dismutase and other enzymes in the plants, enhance metabolic activities of the plants and increase plant stress resistance. The source of the seaweed extract is not specially limited in the present invention, and a source of the seaweed extract well-known to those skilled in the art is available. In embodiments of the present invention, the seaweed extract is purchased from Qingdao Haijingling Seaweed Group Co., Ltd. The compound plant stress resistance agent provided by the present invention includes the trace elements. The trace elements are 0.5-1 part by mass, preferably 0.6-0.8 part, most preferably 0.7 part. The trace elements include copper, manganese, zinc and iron; and a mass ratio of the copper to manganese to zinc to iron is (0.5-1.2):(2.5-3.5):(8-12):(18-22), most preferably 1:3:10:2. The copper is added in the form of amino acid chelated copper; the manganese is added in the form of manganese sulfate; the zinc is added in the form of zinc sulfate; and the iron is added in the form of amino acid chelated iron. The trace elements are beneficial for supplementing trace elements essential for cellular response and increasing the cell viability and provide nutrient elements for plant growth. The source of the trace elements is not specially limited in the present invention, and a source of the trace elements well-known to those skilled in the art is available. The compound plant stress resistance agent provided by the present invention includes the potassium fulvic acid. The potassium fulvic acid is 10-20 parts by mass, preferably 12-18 parts, most preferably 15 parts. The potassium fulvic acid provides potassium. The plants may generally have high stress resistance under
Description
stress conditions if the potassium nutrient element is abundantly supplied. The potassium plays an important role in aspects of enhancing cold resistance, drought resistance, saline-alkali tolerance, resistant to insect pests of the plants. The source of the potassium fulvic acid is not specially limited in the present invention, and a source of the potassium fulvic acid well-known to those skilled in the art is available. In embodiments of the present invention, the potassium fulvic acid is purchased from Shandong Jingyuan Biotechnology Co., Ltd. The present invention provides a preparation method of the compound plant stress resistance agent, including the following steps: (1) manganese and zinc in trace elements were mixed with chitin; the obtained mixture and water were dissolved according to a mass ratio of 1:(1-2); the obtained mixed wet material was mixed with copper and iron in the trace elements and potassium fulvic acid in sequence; and the mixture was dried so as to obtain a first mixture; (2) organic germanium, compound sodium nitrophenolate, choline chloride, allantoin and seaweed extract were mixed so as to obtain a second mixture; and (3) the first mixture in the step (1), the second mixture in the step (2) and ascorbic acid were mixed, thereby obtaining the compound plant stress resistance agent. The mixing method is not specially limited in the present invention, and a mixing solution well-known to those skilled in the art is available. The present invention provides an application of the compound plant stress resistance agent or the compound plant stress resistance agent prepared by the preparation method in plant stress resistance. In the present invention, the plant stress resistance includes salinization resistance. In the present invention, a dilution ratio of the compound plant stress resistance agent is 1:(1000-1500); application modes of the compound plant stress
1 )
Description
resistance agent include root irrigation and foliage spray; and the dilution ratio is preferably 1:1000 during root irrigation, and is preferably 1:1500 during foliage spray. A dosage of the compound plant stress resistance agent is 80-120 g per mu during root irrigation, most preferably 100 g per mu; and the dosage of the compound plant stress resistance agent is 40-60 g per mu during foliage spray, most preferably 50 g per mu. The application mode of root irrigation or foliage spray depends on a crop growth period. For example, the wheat is subjected to root irrigation at a seedling stage and subjected to foliage spray at a jointing stage and a grain-filling stage; the maize is subjected to root irrigation at a seedling stage and subjected to foliage spray at a jointing stage and a booting stage; the peanut is subjected to root irrigation at a seedling stage and subjected to foliage spray in a fruiting period; the cotton is subjected to root irrigation at a seedling stage and subjected to foliage spray at flowering and boll-setting stages; the sweet potato is subjected to root irrigation at a root reviving stage and subjected to foliage spray in a potato piece swelling period; the pepper is subjected to root irrigation at a seedling stage and subjected to foliage spray at blooming and fruit-bearing stages; and the cucumber is subjected to root irrigation at a seedling stage and subjected to foliage spray at an initial flowering stage. The compound plant stress resistance agent and the preparation method and applications thereof provided in the present invention are described below in detail in combination with embodiments. However, the following embodiments shall not be understood as a limitation to the protection scope of the present invention. Embodiment 1 A compound plant stress resistance agent in an adverse environment includes the following components in parts by weight: 0.5 kg of organic germanium, 1.5 kg of ascorbic acid, 1 kg of compound sodium nitrophenolate, 0.4 kg of choline chloride, 1.3 kg of allantoin, 6 kg of chitin, 4 kg of seaweed extract, 0.6 kg of trace
1 1
Description
elements and 15 kg of potassium fulvic acid. A mass ratio of the copper to manganese to zinc to iron in the trace elements is 0.5:3.5:8:22. Preparation steps of the compound stress resistance agent are as follows: (1) ascorbic acid and sodium citrate were fully mixed according to a ratio of 8:1 so as to obtain a materialQ (2) chitin, manganese sulfate and zinc sulfate were uniformly mixed; then the mixture was dissolved with water according to a ratio of 1:1; the solution was uniformly mixed with amino acid chelated copper, amino acid chelated iron and potassium fulvic acid to obtain a mixture; and the mixture was air-dried so as to obtain a material@ (3) organic germanium, compound sodium nitrophenolate, choline chloride, allantoin and seaweed extract were uniformly mixed according to a ratio so as to obtain a mixture material@ (4) the material § the material @and the material @were mixed, thereby obtaining the compound stress resistance agent. Embodiment 2 A compound plant stress resistance agent in an adverse environment includes the following components in parts by weight: 0.6 kg of organic germanium, 1 kg of ascorbic acid, 1.2 kg of compound sodium nitrophenolate, 0.5 kg of choline chloride, 1 kg of allantoin, 5 kg of chitin, 5 kg of seaweed extract, 0.5 kg of trace elements and 16 kg of potassium fulvic acid. A mass ratio of the copper to manganese to zinc to iron in the trace elements is 1.2:2.5:12:18. Preparation steps of the compound stress resistance agent are as follows: (1) a stabilizer potassium citrate and potassium sulfate were uniformly mixed according to a ratio of 1:1; and ascorbic acid was fully mixed with the mixture according to a ratio of 8:1 so as to obtain a materialQ (2) chitin, manganese sulfate and zinc sulfate were uniformly mixed; then the mixture was dissolved with water according to a ratio of 1:1; the solution was
1)
Description
uniformly mixed with amino acid chelated copper, amino acid chelated iron and potassium fulvic acid to obtain a mixture; and the mixture was air-dried so as to obtain a material@ (3) organic germanium, compound sodium nitrophenolate, choline chloride, allantoin and seaweed extract were uniformly mixed according to a ratio so as to obtain a mixture material@ (4) finally, the material the material (Z)ind the material were fully mixed, thereby obtaining the compound stress resistance agent. Embodiment 3 A compound plant stress resistance agent in an adverse environment includes the following components in parts by weight: 0.4 kg of organic germanium, 2 kg of ascorbic acid, 2 kg of compound sodium nitrophenolate, 0.8 kg of choline chloride, 2 kg of allantoin, 10 kg of chitin, 6 kg of seaweed extract, 0.8 kg of trace elements and 10 kg of potassium fulvic acid. A mass ratio of the copper to manganese to zinc to iron in the trace elements is 1:3:10:2. Preparation steps of the compound stress resistance agent are as follows: (1) a stabilizer potassium citrate, potassium sulfate and monopotassium phosphate were uniformly mixed according to a ratio of 2:2:1; and ascorbic acid was fully mixed with the mixture according to a ratio of 9:1 so as to obtain a material (2) chitin, manganese sulfate and zinc sulfate were uniformly mixed; then the mixture was dissolved with water according to a ratio of 1:1; the solution was uniformly mixed with amino acid chelated copper, amino acid chelated iron and potassium fulvic acid to obtain a mixture; and the mixture was air-dried so as to obtain a material@ (3) organic germanium, compound sodium nitrophenolate, choline chloride, allantoin and seaweed extract were uniformly mixed according to a ratio so as to obtain a mixture material@
Description
(4) finally, the material the material (Z)nd the material (5)were fully mixed, thereby obtaining the compound stress resistance agent. Reference example 1 A compound plant stress resistance agent in an adverse environment includes the following components in parts by weight: 0.6 kg of organic germanium, 1 kg of ascorbic acid, and 0.5 kg of compound sodium nitrophenolate. Reference example 2 A compound plant stress resistance agent in an adverse environment includes the following components in parts by weight: 0.8 kg of choline chloride, 1 kg of allantoin and 8 kg of chitin. Reference example 3 A compound plant stress resistance agent in an adverse environment includes the following components in parts by weight: 0.8 kg of organic germanium, 5 kg of seaweed extract, 0.5 kg of trace elements and 15 kg of potassium fulvic acid. Embodiments 4-6 and reference examples 4-6 A using method of the compound plant stress resistance agent prepared in embodiments 1-3 and reference examples 4-6 under adverse environment regulation includes the following steps: the compound stress resistance agent was respectively diluted according to a ratio of 1:1000; the plants were subjected to root irrigation (100 g per mu) at a seedling growth stage, and subjected to foliage spray at other stages when the compound stress resistance agent was diluted according to a ratio of 1:1500 (50 g per mu), e.g., foliage spray was performed at a jointing stage and a grain-filling stage of the wheat, at a jointing stage and a booting stage of the maize, in a fruiting period of the peanut, at flowering and boll-setting stages of the cotton, in a potato piece swelling period of the sweet potato, at blooming and fruit-bearing stages of the pepper, and at an initial flowering stage of the cucumber.
1A
Description
Experimental field selection: saline soil in Nonggao District, Dongying City, secondary saline soil in outdoor vegetable fields of Jinxiang County, Jining and greenhouse vegetable saline soil in Linzi District, Zibo were selected for conducting experiments on the compound plant stress resistance agent prepared in embodiments 1-3 and reference examples 1-3 of the present invention. Experimental objects are specifically as follows: wheat, maize, peanut, cotton and sweet potato in Dongying; pepper in Jinxiang; and cucumber in Linzi. Clear water serves as control. Specific data is as shown in Table 1 as follows: Table 1 Results of yield of different crops applied with the compound plant stress resistance agent in reference examples 1-3 and embodiments 1-3 Treatment Wheat Maize Cotton Peanut Sweet potato Pepper Cucumber
Embodiment 1 378 475 361 413 528 1983 5215
Embodiment 2 381 471 352 409 509 2017 5191
Embodiment 3 385 486 357 421 517 2125 5417
Reference example 1 346 446 331 363 481 1817 4739
Reference example 2 351 439 327 371 473 1869 4681
Reference example 3 339 452 319 377 468 1792 4695
Clear water control 311 381 278 331 451 1510 3972
Notes: the unit is kg per mu; the yield of the peanut is pod weight; and the yield of the sweet potato is weight of dried sweet potatoes. It can be seen from Table 1 that, the yield of the wheat, the maize, the cotton, the peanut, the sweet potato, the pepper and the cucumber in the embodiments is higher than that in the references averagely by 10.4%, 7.1%, 9.5%, 11.9%, 9.3%, 11.8% and 12.1%, and higher than that of the clear water control by 22.6%, 25.3%, 28.3%,25.2%,14.9%,35.2% and 32.8%. Through the above embodiments, the various components of the compound plant stress resistance agent provided by the present invention have synergistic effects while achieving a stress-resistant effect. A stress-resistant effect equivalent
Description
to that of the present invention cannot be achieved by three or several components in the reference examples 1-3. The above only describes preferred embodiments of the present invention. It should be indicated that, several improvements and modifications may be made by those ordinary skilled in the art without departing from the principle of the present invention. These improvements and modifications shall be considered as the protection scope of the present invention.
Claims (10)
1. A compound plant stress resistance agent, comprising the following components in parts by weight: 0.4-0.8 part of organic germanium, 1-3 parts of ascorbic acid, 0.5-3 parts of compound sodium nitrophenolate, 0.2-1 part of choline chloride, 1-2 parts of allantoin, 5-10 parts of chitin, 3-6 parts of seaweed extract, 0.5-1 part of trace elements and 10-20 parts of potassium fulvic acid, wherein the trace elements comprise copper, manganese, zinc and iron; and a mass ratio of the copper to manganese to zinc to iron is (0.2-1.5):(2-4):(7-14):(15-25).
2. The compound plant stress resistance agent according to claim 1, comprising the following components in parts by weight: 0.5-0.7 part of organic germanium, 1.5-2.5 parts of ascorbic acid, 1-2.5 parts of compound sodium nitrophenolate, 0.4-0.8 part of choline chloride, 1.2-1.7 parts of allantoin, 6-8 parts of chitin, 4-5 parts of seaweed extract, 0.6-0.8 part of trace elements and 12-18 parts of potassium fulvic acid, wherein the trace elements comprise copper, manganese, zinc and iron; and a mass ratio of the copper to manganese to zinc to iron is (0.5-1.2):(2.5-3.5):(8-12):(18-22).
3. The compound plant stress resistance agent according to claim 1 or 2, wherein the organic germanium is carboxylethyl germanium sesquioxide; a molecular formula of the carboxylethyl germanium sesquioxide is C 6 HioGe 2 O 7 ;
purity of the carboxylethyl germanium sesquioxide is higher than 99%.
4. The compound plant stress resistance agent according to claim 1 or 2, wherein the chitin is water-soluble chitin.
5. The compound plant stress resistance agent according to claim 1 or 2, wherein the copper is added in the form of amino acid chelated copper; the manganese is added in the form of manganese sulfate; the zinc is added in the form of zinc sulfate; and the iron is added in the form of amino acid chelated iron.
Claims
6. The compound plant stress resistance agent according to claim 1 or 2, wherein the ascorbic acid comprises a stabilizer; content of the stabilizer is 10-20% of the mass of the ascorbic acid.
7. A preparation method of the compound plant stress resistance agent of any one of claims 1-6, comprising the following steps: (1) mixing manganese and zinc in trace elements with chitin; dissolving the obtained mixture and water according to a mass ratio of 1:(1-2); mixing the obtained mixed wet material with copper and iron in the trace elements and potassium fulvic acid in sequence; and drying the mixture so as to obtain a first mixture; (2) mixing organic germanium, compound sodium nitrophenolate, choline chloride, allantoin and seaweed extract so as to obtain a second mixture; (3) mixing the first mixture in the step (1), the second mixture in the step (2) and ascorbic acid, thereby obtaining the compound plant stress resistance agent.
8. An application of the compound plant stress resistance agent of any one of claims 1-6 or the compound plant stress resistance agent prepared by the preparation method of claim 7 in plant stress resistance.
9. The application according to claim 8, wherein the plant stress resistance comprises salinization resistance.
10. The application according to claim 8 or 9, wherein a dilution ratio of the compound plant stress resistance agent is 1:(1000-1500); application modes of the compound plant stress resistance agent comprise root irrigation and foliage spray; a dosage of the compound plant stress resistance agent is 80-120 g per mu during root irrigation; the dosage of the compound plant stress resistance agent is 40-60 g per mu during foliage spray.
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CN114342939A (en) * | 2022-03-17 | 2022-04-15 | 山东华诺联邦农化有限公司 | Strawberry rooting agent and preparation method and application thereof |
CN114395401A (en) * | 2021-12-24 | 2022-04-26 | 神究富硒农业发展(山东)有限公司 | Cell repairing liquid, preparation method and application thereof |
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CN114395401A (en) * | 2021-12-24 | 2022-04-26 | 神究富硒农业发展(山东)有限公司 | Cell repairing liquid, preparation method and application thereof |
CN114395401B (en) * | 2021-12-24 | 2023-11-24 | 神究富硒农业发展(山东)有限公司 | Cell repair liquid, preparation method and application thereof |
CN114342939A (en) * | 2022-03-17 | 2022-04-15 | 山东华诺联邦农化有限公司 | Strawberry rooting agent and preparation method and application thereof |
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