AU2018231559A1 - Extracellular matrix-producing composition using MAST4 gene and preparation method therefor - Google Patents

Extracellular matrix-producing composition using MAST4 gene and preparation method therefor Download PDF

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Publication number
AU2018231559A1
AU2018231559A1 AU2018231559A AU2018231559A AU2018231559A1 AU 2018231559 A1 AU2018231559 A1 AU 2018231559A1 AU 2018231559 A AU2018231559 A AU 2018231559A AU 2018231559 A AU2018231559 A AU 2018231559A AU 2018231559 A1 AU2018231559 A1 AU 2018231559A1
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AU
Australia
Prior art keywords
composition
mast4
fragment
protein
eukaryotic cells
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Abandoned
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AU2018231559A
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English (en)
Inventor
Han Sung Jung
Seong Jin Kim
Satoru Takahashi
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Kim Yeung Won June
Kim Ypsse
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Kim Yeung Won June
Kim Ypsse
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Publication date
Application filed by Kim Yeung Won June, Kim Ypsse filed Critical Kim Yeung Won June
Priority claimed from PCT/KR2018/002763 external-priority patent/WO2018164507A2/ko
Publication of AU2018231559A1 publication Critical patent/AU2018231559A1/en
Priority to AU2023222944A priority Critical patent/AU2023222944B2/en
Abandoned legal-status Critical Current

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    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0652—Cells of skeletal and connective tissues; Mesenchyme
    • C12N5/0655—Chondrocytes; Cartilage
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies
    • C07K16/40—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies against enzymes
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K38/00—Medicinal preparations containing peptides
    • A61K38/16—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • A61K38/17—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • A61K38/18—Growth factors; Growth regulators
    • A61K38/1841—Transforming growth factor [TGF]
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    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/102—Mutagenizing nucleic acids
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    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/113—Non-coding nucleic acids modulating the expression of genes, e.g. antisense oligonucleotides; Antisense DNA or RNA; Triplex- forming oligonucleotides; Catalytic nucleic acids, e.g. ribozymes; Nucleic acids used in co-suppression or gene silencing
    • C12N15/1137—Non-coding nucleic acids modulating the expression of genes, e.g. antisense oligonucleotides; Antisense DNA or RNA; Triplex- forming oligonucleotides; Catalytic nucleic acids, e.g. ribozymes; Nucleic acids used in co-suppression or gene silencing against enzymes
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    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
    • C12N15/90—Stable introduction of foreign DNA into chromosome
    • C12N15/902—Stable introduction of foreign DNA into chromosome using homologous recombination
    • C12N15/907—Stable introduction of foreign DNA into chromosome using homologous recombination in mammalian cells
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    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0652—Cells of skeletal and connective tissues; Mesenchyme
    • C12N5/0656—Adult fibroblasts
    • C—CHEMISTRY; METALLURGY
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    • C12N9/10—Transferases (2.)
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    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
    • C12N9/22—Ribonucleases [RNase]; Deoxyribonucleases [DNase]
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    • C12Y207/00—Transferases transferring phosphorus-containing groups (2.7)
    • C12Y207/11—Protein-serine/threonine kinases (2.7.11)
    • C12Y207/11001—Non-specific serine/threonine protein kinase (2.7.11.1), i.e. casein kinase or checkpoint kinase
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K2217/00—Genetically modified animals
    • A01K2217/07—Animals genetically altered by homologous recombination
    • A01K2217/075—Animals genetically altered by homologous recombination inducing loss of function, i.e. knock out
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K2227/00—Animals characterised by species
    • A01K2227/10—Mammal
    • A01K2227/105—Murine
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K2267/00—Animals characterised by purpose
    • A01K2267/03—Animal model, e.g. for test or diseases
    • A01K2267/035—Animal model for multifactorial diseases
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    • C12N2310/00—Structure or type of the nucleic acid
    • C12N2310/10—Type of nucleic acid
    • C12N2310/14—Type of nucleic acid interfering nucleic acids [NA]
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    • C12N2310/00—Structure or type of the nucleic acid
    • C12N2310/10—Type of nucleic acid
    • C12N2310/20—Type of nucleic acid involving clustered regularly interspaced short palindromic repeats [CRISPR]
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    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/65—MicroRNA
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    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/70—Enzymes
    • C12N2501/72—Transferases [EC 2.]
    • C12N2501/727—Kinases (EC 2.7.)
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    • C12N2800/00—Nucleic acids vectors
    • C12N2800/80—Vectors containing sites for inducing double-stranded breaks, e.g. meganuclease restriction sites

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  • Genetics & Genomics (AREA)
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  • Bioinformatics & Cheminformatics (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
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  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
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  • Medicinal Chemistry (AREA)
  • Biophysics (AREA)
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  • Plant Pathology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Rheumatology (AREA)
  • Immunology (AREA)
  • Cell Biology (AREA)
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  • Veterinary Medicine (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Pharmacology & Pharmacy (AREA)
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  • Animal Behavior & Ethology (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Mycology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
  • Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
  • Peptides Or Proteins (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)
AU2018231559A 2017-03-08 2018-03-08 Extracellular matrix-producing composition using MAST4 gene and preparation method therefor Abandoned AU2018231559A1 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
AU2023222944A AU2023222944B2 (en) 2017-03-08 2023-08-31 Extracellular matrix-producing composition using MAST4 gene and preparation method therefor

Applications Claiming Priority (5)

Application Number Priority Date Filing Date Title
KR10-2017-0029607 2017-03-08
KR20170029607 2017-03-08
KR1020180027111A KR102588627B1 (ko) 2017-03-08 2018-03-07 Mast4 유전자를 이용한 세포외 기질 생산용 조성물 및 그 제조방법
KR10-2018-0027111 2018-03-07
PCT/KR2018/002763 WO2018164507A2 (ko) 2017-03-08 2018-03-08 Mast4 유전자를 이용한 세포외 기질 생산용 조성물 및 그 제조방법

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AU2023222944A Division AU2023222944B2 (en) 2017-03-08 2023-08-31 Extracellular matrix-producing composition using MAST4 gene and preparation method therefor

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AU2018231559A1 true AU2018231559A1 (en) 2019-10-31

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AU2023222944A Active AU2023222944B2 (en) 2017-03-08 2023-08-31 Extracellular matrix-producing composition using MAST4 gene and preparation method therefor

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US (2) US11180573B2 (https=)
EP (1) EP3594324A4 (https=)
JP (3) JP7154238B2 (https=)
KR (1) KR102588627B1 (https=)
CN (1) CN110612348B (https=)
AU (2) AU2018231559A1 (https=)
CA (1) CA3055729A1 (https=)

Families Citing this family (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2021146566A1 (en) * 2020-01-16 2021-07-22 The Johns Hopkins University Engineered fibroblasts as cell therapy to treat cancer via tumor stroma stabilization
US20240299579A1 (en) * 2020-07-23 2024-09-12 Orthobio Therapeutics, Inc. Gene editing to improve joint function
KR20240147995A (ko) * 2022-02-04 2024-10-10 김성진 뼈 및 연골을 제조하는 방법

Family Cites Families (15)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5055298A (en) * 1987-12-01 1991-10-08 Chemisches Laboratorium Dr. Kurt Richter Gmbh Cosmetic composition comprising an extracellular connective tissue matrix
US6528052B1 (en) * 2000-09-29 2003-03-04 The Board Of Trustees Of The Leland Stanford Junior University Method for in vivo ex vivo and in vitro repair and regeneration of cartilage and collagen and bone remodeling
US20060153797A1 (en) * 2004-06-11 2006-07-13 Bortolotto Susan K Tissue material and matrix
KR100614903B1 (ko) * 2004-08-06 2006-08-25 테고사이언스 (주) 케라티노사이트 및/또는 섬유아세포를 함유하는 화장용조성물
EP1894581A1 (en) * 2006-08-31 2008-03-05 CellCoTec B.V. Repair of cartilage tissue using a matrix gel containing chondrocytes
ES2441966T3 (es) * 2008-03-31 2014-02-07 Scarcell Therapeutics Procedimiento para el tratamiento cosmético del fotoenvejecimiento de la piel
US8728730B2 (en) 2009-09-03 2014-05-20 Genentech, Inc. Methods for treating, diagnosing, and monitoring rheumatoid arthritis
US10301620B2 (en) * 2010-12-22 2019-05-28 Murdoch Childrens Research Institute Method of treatment
EP2753692B1 (en) * 2011-09-07 2019-03-27 3-D Matrix Ltd. Microrna-based methods and assays for osteosarcoma
US9254311B2 (en) 2012-04-02 2016-02-09 Moderna Therapeutics, Inc. Modified polynucleotides for the production of proteins
KR102039311B1 (ko) 2012-09-07 2019-11-04 주식회사 마크로젠 Ａｘｌ을 포함하는 융합 단백질 및 이를 포함하는 암 진단용 조성물
EP3043825A4 (en) 2013-09-09 2017-05-03 Figene, LLC Gene therapy for the regeneration of chondrocytes or cartilage type cells
KR20160111366A (ko) * 2013-11-14 2016-09-26 더마체 랩스 엘엘씨 섬유아세포 혼합물 및 그 제조 방법 및 사용 방법
US10702527B2 (en) 2015-06-12 2020-07-07 Dana-Farber Cancer Institute, Inc. Combination therapy of transcription inhibitors and kinase inhibitors
JP2019501943A (ja) 2016-01-14 2019-01-24 スパイナルサイト， エルエルシー 軟骨細胞または軟骨型細胞を再生するための細胞混成物

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Publication number Publication date
JP2024116197A (ja) 2024-08-27
JP2022188178A (ja) 2022-12-20
AU2023222944B2 (en) 2026-03-05
US11180573B2 (en) 2021-11-23
KR102588627B1 (ko) 2023-10-16
US20200239596A1 (en) 2020-07-30
JP7154238B2 (ja) 2022-10-17
CN110612348B (zh) 2024-02-09
KR20180103015A (ko) 2018-09-18
CA3055729A1 (en) 2018-09-13
US20220220221A1 (en) 2022-07-14
CN110612348A (zh) 2019-12-24
JP2020513849A (ja) 2020-05-21
EP3594324A2 (en) 2020-01-15
AU2023222944A1 (en) 2023-11-23
JP7498239B2 (ja) 2024-06-11
EP3594324A4 (en) 2021-01-20

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