AU2016348439B2 - Combinatorial sets of nucleic acid barcodes for analysis of nucleic acids associated with single cells - Google Patents

Combinatorial sets of nucleic acid barcodes for analysis of nucleic acids associated with single cells Download PDF

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AU2016348439B2
AU2016348439B2 AU2016348439A AU2016348439A AU2016348439B2 AU 2016348439 B2 AU2016348439 B2 AU 2016348439B2 AU 2016348439 A AU2016348439 A AU 2016348439A AU 2016348439 A AU2016348439 A AU 2016348439A AU 2016348439 B2 AU2016348439 B2 AU 2016348439B2
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nucleic acid
barcode
barcodes
sequence
construct
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AU2016348439A1 (en
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Gary WITHEY
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Atreca Inc
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    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6806—Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
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    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C12N15/1065—Preparation or screening of tagged libraries, e.g. tagged microorganisms by STM-mutagenesis, tagged polynucleotides, gene tags
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    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
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    • C12Q2523/00—Reactions characterised by treatment of reaction samples
    • C12Q2523/30—Characterised by physical treatment
    • C12Q2523/319—Photocleavage, photolysis, photoactivation
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12Q2525/00—Reactions involving modified oligonucleotides, nucleic acids, or nucleotides
    • C12Q2525/10—Modifications characterised by
    • C12Q2525/173—Modifications characterised by incorporating a polynucleotide run, e.g. polyAs, polyTs
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12Q2525/00—Reactions involving modified oligonucleotides, nucleic acids, or nucleotides
    • C12Q2525/10—Modifications characterised by
    • C12Q2525/191—Modifications characterised by incorporating an adaptor
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    • C12Q2535/00—Reactions characterised by the assay type for determining the identity of a nucleotide base or a sequence of oligonucleotides
    • C12Q2535/122—Massive parallel sequencing
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    • C12Q2563/00—Nucleic acid detection characterized by the use of physical, structural and functional properties
    • C12Q2563/149—Particles, e.g. beads
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    • C12Q2563/00—Nucleic acid detection characterized by the use of physical, structural and functional properties
    • C12Q2563/159—Microreactors, e.g. emulsion PCR or sequencing, droplet PCR, microcapsules, i.e. non-liquid containers with a range of different permeability's for different reaction components
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    • C12Q2563/00—Nucleic acid detection characterized by the use of physical, structural and functional properties
    • C12Q2563/179—Nucleic acid detection characterized by the use of physical, structural and functional properties the label being a nucleic acid
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    • C12Q2563/00—Nucleic acid detection characterized by the use of physical, structural and functional properties
    • C12Q2563/185—Nucleic acid dedicated to use as a hidden marker/bar code, e.g. inclusion of nucleic acids to mark art objects or animals
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12Q2565/00—Nucleic acid analysis characterised by mode or means of detection
    • C12Q2565/50—Detection characterised by immobilisation to a surface
    • C12Q2565/514—Detection characterised by immobilisation to a surface characterised by the use of the arrayed oligonucleotides as identifier tags, e.g. universal addressable array, anti-tag or tag complement array

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  • Chemical & Material Sciences (AREA)
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  • Wood Science & Technology (AREA)
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  • Microbiology (AREA)
  • Biochemistry (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Analytical Chemistry (AREA)
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  • Crystallography & Structural Chemistry (AREA)
  • Plant Pathology (AREA)
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  • Chemical Kinetics & Catalysis (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Toxicology (AREA)
AU2016348439A 2015-11-04 2016-11-04 Combinatorial sets of nucleic acid barcodes for analysis of nucleic acids associated with single cells Active AU2016348439B2 (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US201562250900P 2015-11-04 2015-11-04
US62/250,900 2015-11-04
PCT/US2016/060576 WO2017079593A1 (en) 2015-11-04 2016-11-04 Combinatorial sets of nucleic acid barcodes for analysis of nucleic acids associated with single cells

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AU2016348439A1 AU2016348439A1 (en) 2018-05-10
AU2016348439B2 true AU2016348439B2 (en) 2023-03-09

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EP (1) EP3371309B1 (enExample)
JP (1) JP7064439B2 (enExample)
KR (1) KR20180097536A (enExample)
CN (1) CN108473984A (enExample)
AU (1) AU2016348439B2 (enExample)
CA (1) CA3004310A1 (enExample)
SG (1) SG11201803646YA (enExample)
WO (1) WO2017079593A1 (enExample)

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