AU2016280700B2 - Glucosyltransferase amino acid sequence motifs for producing linear poly alpha-1,3-glucan - Google Patents
Glucosyltransferase amino acid sequence motifs for producing linear poly alpha-1,3-glucan Download PDFInfo
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- AU2016280700B2 AU2016280700B2 AU2016280700A AU2016280700A AU2016280700B2 AU 2016280700 B2 AU2016280700 B2 AU 2016280700B2 AU 2016280700 A AU2016280700 A AU 2016280700A AU 2016280700 A AU2016280700 A AU 2016280700A AU 2016280700 B2 AU2016280700 B2 AU 2016280700B2
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- seq
- amino acid
- glucan
- gtf
- motif
- Prior art date
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/10—Transferases (2.)
- C12N9/1048—Glycosyltransferases (2.4)
- C12N9/1051—Hexosyltransferases (2.4.1)
-
- C—CHEMISTRY; METALLURGY
- C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
- C08B—POLYSACCHARIDES; DERIVATIVES THEREOF
- C08B37/00—Preparation of polysaccharides not provided for in groups C08B1/00 - C08B35/00; Derivatives thereof
- C08B37/0006—Homoglycans, i.e. polysaccharides having a main chain consisting of one single sugar, e.g. colominic acid
- C08B37/0009—Homoglycans, i.e. polysaccharides having a main chain consisting of one single sugar, e.g. colominic acid alpha-D-Glucans, e.g. polydextrose, alternan, glycogen; (alpha-1,4)(alpha-1,6)-D-Glucans; (alpha-1,3)(alpha-1,4)-D-Glucans, e.g. isolichenan or nigeran; (alpha-1,4)-D-Glucans; (alpha-1,3)-D-Glucans, e.g. pseudonigeran; Derivatives thereof
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
- C12N15/102—Mutagenizing nucleic acids
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P19/00—Preparation of compounds containing saccharide radicals
- C12P19/04—Polysaccharides, i.e. compounds containing more than five saccharide radicals attached to each other by glycosidic bonds
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P19/00—Preparation of compounds containing saccharide radicals
- C12P19/18—Preparation of compounds containing saccharide radicals produced by the action of a glycosyl transferase, e.g. alpha-, beta- or gamma-cyclodextrins
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/48—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving transferase
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Y—ENZYMES
- C12Y204/00—Glycosyltransferases (2.4)
- C12Y204/01—Hexosyltransferases (2.4.1)
- C12Y204/01027—DNA beta-glucosyltransferase (2.4.1.27)
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- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Health & Medical Sciences (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Genetics & Genomics (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Health & Medical Sciences (AREA)
- Biochemistry (AREA)
- General Engineering & Computer Science (AREA)
- Biotechnology (AREA)
- Microbiology (AREA)
- Molecular Biology (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Biomedical Technology (AREA)
- General Chemical & Material Sciences (AREA)
- Medicinal Chemistry (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Biophysics (AREA)
- Physics & Mathematics (AREA)
- Materials Engineering (AREA)
- Polymers & Plastics (AREA)
- Immunology (AREA)
- Analytical Chemistry (AREA)
- Plant Pathology (AREA)
- Crystallography & Structural Chemistry (AREA)
- Enzymes And Modification Thereof (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Applications Claiming Priority (5)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US201562180788P | 2015-06-17 | 2015-06-17 | |
| US201562180779P | 2015-06-17 | 2015-06-17 | |
| US62/180,779 | 2015-06-17 | ||
| US62/180,788 | 2015-06-17 | ||
| PCT/US2016/037673 WO2016205401A1 (en) | 2015-06-17 | 2016-06-15 | Glucosyltransferase amino acid sequence motifs for producing linear poly alpha-1,3-glucan |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| AU2016280700A1 AU2016280700A1 (en) | 2018-01-04 |
| AU2016280700B2 true AU2016280700B2 (en) | 2020-12-24 |
Family
ID=56194626
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| AU2016280700A Active AU2016280700B2 (en) | 2015-06-17 | 2016-06-15 | Glucosyltransferase amino acid sequence motifs for producing linear poly alpha-1,3-glucan |
Country Status (7)
| Country | Link |
|---|---|
| US (4) | US9988610B2 (enExample) |
| EP (2) | EP3310906B1 (enExample) |
| JP (1) | JP2018518179A (enExample) |
| CN (1) | CN107922958A (enExample) |
| AU (1) | AU2016280700B2 (enExample) |
| CA (1) | CA2989337A1 (enExample) |
| WO (2) | WO2016205391A1 (enExample) |
Families Citing this family (49)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US10895028B2 (en) * | 2015-12-14 | 2021-01-19 | Dupont Industrial Biosciences Usa, Llc | Nonwoven glucan webs |
| WO2018052942A1 (en) | 2016-09-14 | 2018-03-22 | E. I. Du Pont De Nemours And Company | Engineered glucosyltransferases |
| CN110291113B (zh) | 2017-02-16 | 2022-03-04 | 营养与生物科学美国4公司 | 交联的右旋糖酐和交联的右旋糖酐-聚α-1，3-葡聚糖接枝共聚物 |
| CN110651049A (zh) | 2017-05-23 | 2020-01-03 | 纳幕尔杜邦公司 | α-1,3-葡聚糖的酶促生产 |
| WO2019055373A1 (en) | 2017-09-13 | 2019-03-21 | E. I. Du Pont De Nemours And Company | Engineered glucosyltransferases |
| EP3668970A1 (en) | 2017-09-13 | 2020-06-24 | DuPont Industrial Biosciences USA, LLC | Engineered glucosyltransferases |
| EP3694891B1 (en) | 2017-10-13 | 2022-08-24 | Nutrition & Biosciences USA 4, Inc. | Flowable bulk granular polysaccharide |
| CN111566128B (zh) | 2017-11-10 | 2022-06-21 | 营养与生物科学美国4公司 | 独特形态的多糖 |
| WO2019118674A1 (en) | 2017-12-14 | 2019-06-20 | E. I. Du Pont De Nemours And Company | Alpha-1,3-glucan graft copolymers |
| AU2019230061B2 (en) | 2018-03-09 | 2025-05-15 | Nutrition & Biosciences USA 4, Inc. | Engineered glucosyltransferases |
| CN113574074B (zh) | 2018-10-25 | 2023-03-21 | 营养与生物科学美国第四公司 | α-1,3-葡聚糖接枝共聚物 |
| US20220033531A1 (en) | 2018-12-17 | 2022-02-03 | Dupont Industrial Biosciences Usa,Llc | Polysaccharide Derivatives and Compositions Comprising Same |
| US12618051B2 (en) | 2019-07-09 | 2026-05-05 | Nutrition & Biosciences USA 4, Inc. | Engineered alpha-1,3 branching enzymes |
| US12389916B2 (en) | 2019-08-16 | 2025-08-19 | Nutrition & Biosciences USA 4, Inc. | Flour- and meal-based food products comprising insoluble alpha-1,3-glucan |
| EP4055063A1 (en) | 2019-11-06 | 2022-09-14 | Nutrition & Biosciences USA 4, Inc. | Highly crystalline alpha-1,3-glucan |
| CN111019918B (zh) * | 2019-11-20 | 2023-05-09 | 广东广业清怡食品科技股份有限公司 | 一种糖基转移酶突变体及其应用 |
| CN115052905B (zh) | 2020-02-04 | 2024-06-11 | 营养与生物科学美国4公司 | 包含α-1,3糖苷键的不溶性α-葡聚糖的水性分散体 |
| EP4161972A1 (en) | 2020-06-04 | 2023-04-12 | Nutrition & Biosciences USA 4, Inc. | Dextran-alpha-glucan graft copolymers and derivatives thereof |
| CN116134055A (zh) | 2020-06-10 | 2023-05-16 | 营养与生物科学美国4公司 | 聚α-1,6-葡聚糖衍生物和包含其的组合物 |
| WO2022178075A1 (en) | 2021-02-19 | 2022-08-25 | Nutrition & Biosciences USA 4, Inc. | Oxidized polysaccharide derivatives |
| EP4334363A1 (en) | 2021-05-04 | 2024-03-13 | Nutrition & Biosciences USA 4, Inc. | Compositions comprising insoluble alpha-glucan |
| US20240239921A1 (en) | 2021-05-04 | 2024-07-18 | Nutrition & Biosciences USA 4, Inc. | Compositions comprising oxidized insoluble alpha-glucan |
| US20240307288A1 (en) | 2021-07-13 | 2024-09-19 | Nutrition & Biosciences USA 4, Inc. | Cationic glucan ester derivatives |
| AU2022354202A1 (en) | 2021-09-30 | 2024-03-14 | International N&H Denmark Aps | Method for reducing sugar in food stuff |
| EP4426362A1 (en) | 2021-11-05 | 2024-09-11 | Nutrition & Biosciences USA 4, Inc. | Glucan derivatives for microbial control |
| US20250032390A1 (en) | 2021-12-16 | 2025-01-30 | Nutrition & Biosciences USA 4, Inc. | Compositions comprising cationic alpha-glucan ethers in aqueous polar organic solvents |
| WO2023137258A1 (en) | 2022-01-12 | 2023-07-20 | Nutrition & Biosciences USA 4, Inc. | Coating compositions comprising rubber and insoluble alpha-glucan |
| CA3246295A1 (en) | 2022-03-21 | 2023-09-28 | Nutrition & Biosciences USA 4, Inc. | COMPOSITIONS CONTAINING AN INSOLUBLE ALPHA-GLUCANE |
| WO2024015769A1 (en) | 2022-07-11 | 2024-01-18 | Nutrition & Biosciences USA 4, Inc. | Amphiphilic glucan ester derivatives |
| CN120225168A (zh) | 2022-10-14 | 2025-06-27 | 营养与生物科学美国第四公司 | 包含水、阳离子α-1,6-葡聚糖醚和有机溶剂的组合物 |
| WO2024086560A1 (en) | 2022-10-17 | 2024-04-25 | International N&H Denmark Aps | Method for improving flavor in plant-based food stuff |
| US20250339361A1 (en) | 2022-11-02 | 2025-11-06 | Nutrition & Biosciences USA 4, Inc. | Compositions comprising xanthan gum and crystalline alpha-1,3-glucan |
| WO2024112740A1 (en) | 2022-11-23 | 2024-05-30 | Nutrition & Biosciences USA 4, Inc. | Hygienic treatment of surfaces with compositions comprising hydrophobically modified alpha-glucan derivative |
| US20260209394A1 (en) | 2022-12-16 | 2026-07-23 | Nutrition & Biosciences USA 4, Inc. | Esterification of alpha-glucan comprising alpha-1,6 glycosidic linkages |
| WO2024206631A1 (en) | 2023-03-29 | 2024-10-03 | International N&H Denmark Aps | Methods for modifying texture in foodstuff via preferably in situ produced alpha-glucan |
| WO2024228945A1 (en) | 2023-05-03 | 2024-11-07 | Nutrition & Biosciences USA 4, Inc. | Controlling bacterial cell adhesion with alpha-glucan comprising alpha-1,6 glycosidic linkages |
| WO2025006691A2 (en) | 2023-06-30 | 2025-01-02 | Nutrition & Biosciences USA 4, Inc. | Porous alpha-1,3-glucan compositions |
| CN122249471A (zh) | 2023-09-29 | 2026-06-19 | 美国丹尼斯科公司 | 交联的α-葡聚糖衍生物 |
| WO2025072417A1 (en) | 2023-09-29 | 2025-04-03 | Nutrition & Biosciences USA 4, Inc. | Polysaccharide derivatives |
| EP4784633A1 (en) | 2023-09-29 | 2026-08-05 | Nutrition & Biosciences USA 4, Inc. | Polysaccharide derivatives |
| CN122295376A (zh) | 2023-11-28 | 2026-06-26 | 营养与生物科学美国第四公司 | 包含α-1,6糖苷键的α-葡聚糖的酯化 |
| WO2025198996A1 (en) | 2024-03-19 | 2025-09-25 | Danisco Us Inc. | Method for improving flavor in foodstuff |
| WO2025199079A1 (en) | 2024-03-20 | 2025-09-25 | Nutrition & Biosciences USA 4, Inc. | Esterification of alpha-glucan comprising alpha-1,6 glycosidic linkages |
| WO2026015598A2 (en) | 2024-07-11 | 2026-01-15 | International N&H Denmark Aps | Method to provide improved texture and stable sucrose levels in foodstuff with glucosyltransferase |
| WO2026024663A1 (en) | 2024-07-24 | 2026-01-29 | Nutrition & Biosciences USA 4, Inc. | Compositions comprising alpha-glucan with alpha-1,3 glycosidic linkages |
| WO2026039397A1 (en) | 2024-08-13 | 2026-02-19 | Nutrition & Biosciences USA 4, Inc. | Alpha-glucan graft copolymer derivatives |
| WO2026076090A1 (en) | 2024-10-02 | 2026-04-09 | Danisco Usa Inc. | Beta-1,3-glucan compositions |
| WO2026102382A2 (en) | 2024-11-11 | 2026-05-15 | International N&H Denmark Aps | Method for reducing sugar in foodstuff through conversion of fructose with epimerase |
| WO2026112560A1 (en) | 2024-11-25 | 2026-05-28 | International N&H Denmark Aps | Use of glucosyltransferase in dough preparation |
Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20140087431A1 (en) * | 2012-09-25 | 2014-03-27 | E I Du Pont De Nemours And Company | Glucosyltransferase enzymes for production of glucan polymers |
Family Cites Families (16)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP1001736A1 (en) | 1996-10-11 | 2000-05-24 | Novo Nordisk A/S | STARCH BINDING DOMAINS (SBDs) FOR ORAL CARE PRODUCTS |
| US5952205A (en) | 1998-02-06 | 1999-09-14 | Neose Technologies, Inc. | Process for processing sucrose into glucose and fructose |
| KR20010101647A (ko) | 1999-01-25 | 2001-11-14 | 메리 이. 보울러 | 폴리사카라이드 섬유 |
| US6867026B2 (en) * | 2000-05-25 | 2005-03-15 | Nederlandse Organisatie Voor Toegepast Natuurwetenschappelijk Onderzoek Tno | Glucosyltransferases |
| US7524645B2 (en) * | 2004-12-14 | 2009-04-28 | Centre National De La Recherche Scientifique (Cnrs) | Fully active alternansucrases partially deleted in its carboxy-terminal and amino-terminal domains and mutants thereof |
| EP1679374A1 (en) | 2005-01-10 | 2006-07-12 | Bayer CropScience GmbH | Transformed plant expressing a mutansucrase and synthesizing a modified starch |
| KR101540230B1 (ko) * | 2007-04-26 | 2015-07-29 | 가부시기가이샤하야시바라 | 분기 α-글루칸, 이를 생성하는 α-글루코실 전이 효소, 및 그의 제조 방법 및 용도 |
| US7871802B2 (en) * | 2007-10-31 | 2011-01-18 | E.I. Du Pont De Nemours And Company | Process for enzymatically converting glycolonitrile to glycolic acid |
| US8642757B2 (en) | 2011-09-09 | 2014-02-04 | E I Du Pont De Nemours And Company | High titer production of highly linear poly (α 1,3 glucan) |
| US9080195B2 (en) | 2011-09-09 | 2015-07-14 | E I Du Pont De Nemours And Company | High titer production of poly (α 1,3 glucan) |
| WO2013096502A1 (en) | 2011-12-19 | 2013-06-27 | E. I. Du Pont De Nemours And Company | INCREASED POLY (α 1, 3 GLUCAN) YIELD USING BORIC ACID |
| US8962282B2 (en) | 2011-12-19 | 2015-02-24 | E I Du Pont De Nemours And Company | Increased poly (alpha 1,3 glucan) yield using tetraborate |
| EP2821482A1 (en) * | 2013-07-05 | 2015-01-07 | Roche Diagniostics GmbH | N-terminally truncated glycosyltransferases |
| WO2015123327A1 (en) | 2014-02-14 | 2015-08-20 | E. I. Du Pont De Nemours And Company | Glucosyltransferase enzymes for production of glucan polymers |
| EP3105256A1 (en) | 2014-02-14 | 2016-12-21 | E. I. du Pont de Nemours and Company | Poly-alpha-1,3-1,6-glucans for viscosity modification |
| AU2015236336B2 (en) * | 2014-03-25 | 2018-07-05 | Nutrition & Biosciences USA 4, Inc. | Production of glucan polymers from alternate sucrose sources |
-
2016
- 2016-06-15 WO PCT/US2016/037661 patent/WO2016205391A1/en not_active Ceased
- 2016-06-15 CN CN201680047380.1A patent/CN107922958A/zh active Pending
- 2016-06-15 US US15/182,778 patent/US9988610B2/en active Active
- 2016-06-15 EP EP16734785.5A patent/EP3310906B1/en active Active
- 2016-06-15 US US15/182,747 patent/US20170002335A1/en not_active Abandoned
- 2016-06-15 CA CA2989337A patent/CA2989337A1/en not_active Abandoned
- 2016-06-15 AU AU2016280700A patent/AU2016280700B2/en active Active
- 2016-06-15 EP EP16731767.6A patent/EP3310923A1/en active Pending
- 2016-06-15 WO PCT/US2016/037673 patent/WO2016205401A1/en not_active Ceased
- 2016-06-15 JP JP2017565150A patent/JP2018518179A/ja active Pending
-
2018
- 2018-04-18 US US15/955,923 patent/US10508268B2/en active Active
- 2018-11-14 US US16/190,231 patent/US10633638B2/en not_active Expired - Fee Related
Patent Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20140087431A1 (en) * | 2012-09-25 | 2014-03-27 | E I Du Pont De Nemours And Company | Glucosyltransferase enzymes for production of glucan polymers |
Non-Patent Citations (1)
| Title |
|---|
| GenBank Accession No. WP_013990740.1 27 May 2013 * |
Also Published As
| Publication number | Publication date |
|---|---|
| CA2989337A1 (en) | 2016-12-22 |
| US20170002336A1 (en) | 2017-01-05 |
| CN107922958A (zh) | 2018-04-17 |
| WO2016205401A1 (en) | 2016-12-22 |
| US9988610B2 (en) | 2018-06-05 |
| EP3310906A1 (en) | 2018-04-25 |
| AU2016280700A1 (en) | 2018-01-04 |
| EP3310906B1 (en) | 2020-05-06 |
| US10633638B2 (en) | 2020-04-28 |
| US10508268B2 (en) | 2019-12-17 |
| WO2016205391A1 (en) | 2016-12-22 |
| EP3310923A1 (en) | 2018-04-25 |
| US20170002335A1 (en) | 2017-01-05 |
| US20190112585A1 (en) | 2019-04-18 |
| JP2018518179A (ja) | 2018-07-12 |
| US20180305672A1 (en) | 2018-10-25 |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PC1 | Assignment before grant (sect. 113) |
Owner name: NUTRITION & BIOSCIENCES USA 4, INC. Free format text: FORMER APPLICANT(S): E I DU PONT DE NEMOURS AND COMPANY |
|
| FGA | Letters patent sealed or granted (standard patent) |