AU2016280700B2 - Glucosyltransferase amino acid sequence motifs for producing linear poly alpha-1,3-glucan - Google Patents

Glucosyltransferase amino acid sequence motifs for producing linear poly alpha-1,3-glucan Download PDF

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AU2016280700B2
AU2016280700B2 AU2016280700A AU2016280700A AU2016280700B2 AU 2016280700 B2 AU2016280700 B2 AU 2016280700B2 AU 2016280700 A AU2016280700 A AU 2016280700A AU 2016280700 A AU2016280700 A AU 2016280700A AU 2016280700 B2 AU2016280700 B2 AU 2016280700B2
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seq
amino acid
glucan
gtf
motif
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AU2016280700A1 (en
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Richard R. Bott
Yefim Brun
Mark S. Payne
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Nutrition and Biosciences USA 4 Inc
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/1048—Glycosyltransferases (2.4)
    • C12N9/1051—Hexosyltransferases (2.4.1)
    • C—CHEMISTRY; METALLURGY
    • C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
    • C08B—POLYSACCHARIDES; DERIVATIVES THEREOF
    • C08B37/00—Preparation of polysaccharides not provided for in groups C08B1/00 - C08B35/00; Derivatives thereof
    • C08B37/0006—Homoglycans, i.e. polysaccharides having a main chain consisting of one single sugar, e.g. colominic acid
    • C08B37/0009—Homoglycans, i.e. polysaccharides having a main chain consisting of one single sugar, e.g. colominic acid alpha-D-Glucans, e.g. polydextrose, alternan, glycogen; (alpha-1,4)(alpha-1,6)-D-Glucans; (alpha-1,3)(alpha-1,4)-D-Glucans, e.g. isolichenan or nigeran; (alpha-1,4)-D-Glucans; (alpha-1,3)-D-Glucans, e.g. pseudonigeran; Derivatives thereof
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/102—Mutagenizing nucleic acids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00—Preparation of compounds containing saccharide radicals
    • C12P19/04—Polysaccharides, i.e. compounds containing more than five saccharide radicals attached to each other by glycosidic bonds
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00—Preparation of compounds containing saccharide radicals
    • C12P19/18—Preparation of compounds containing saccharide radicals produced by the action of a glycosyl transferase, e.g. alpha-, beta- or gamma-cyclodextrins
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/48—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving transferase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y204/00—Glycosyltransferases (2.4)
    • C12Y204/01—Hexosyltransferases (2.4.1)
    • C12Y204/01027—DNA beta-glucosyltransferase (2.4.1.27)

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  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Health & Medical Sciences (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Genetics & Genomics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
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  • Biomedical Technology (AREA)
  • General Chemical & Material Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Materials Engineering (AREA)
  • Polymers & Plastics (AREA)
  • Analytical Chemistry (AREA)
  • Immunology (AREA)
  • Plant Pathology (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
AU2016280700A 2015-06-17 2016-06-15 Glucosyltransferase amino acid sequence motifs for producing linear poly alpha-1,3-glucan Active AU2016280700B2 (en)

Applications Claiming Priority (5)

Application Number Priority Date Filing Date Title
US201562180788P 2015-06-17 2015-06-17
US201562180779P 2015-06-17 2015-06-17
US62/180,788 2015-06-17
US62/180,779 2015-06-17
PCT/US2016/037673 WO2016205401A1 (en) 2015-06-17 2016-06-15 Glucosyltransferase amino acid sequence motifs for producing linear poly alpha-1,3-glucan

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AU2016280700A1 AU2016280700A1 (en) 2018-01-04
AU2016280700B2 true AU2016280700B2 (en) 2020-12-24

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US (4) US20170002335A1 (cg-RX-API-DMAC7.html)
EP (2) EP3310923A1 (cg-RX-API-DMAC7.html)
JP (1) JP2018518179A (cg-RX-API-DMAC7.html)
CN (1) CN107922958A (cg-RX-API-DMAC7.html)
AU (1) AU2016280700B2 (cg-RX-API-DMAC7.html)
CA (1) CA2989337A1 (cg-RX-API-DMAC7.html)
WO (2) WO2016205391A1 (cg-RX-API-DMAC7.html)

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AU2018272823B2 (en) * 2017-05-23 2024-05-09 Nutrition & Biosciences USA 4, Inc. Enzymatic production of alpha-1,3-glucan
AU2018334513B2 (en) * 2017-09-13 2025-02-20 Nutrition & Biosciences USA 4, Inc. Engineered glucosyltransferases
EP3668970A1 (en) 2017-09-13 2020-06-24 DuPont Industrial Biosciences USA, LLC Engineered glucosyltransferases
WO2019075301A1 (en) 2017-10-13 2019-04-18 E. I. Du Pont De Nemours And Company Flowable bulk granular polysaccharide
CN111566128B (zh) 2017-11-10 2022-06-21 营养与生物科学美国4公司 独特形态的多糖
JP2021507955A (ja) 2017-12-14 2021-02-25 デュポン・インダストリアル・バイオサイエンシーズ・ユーエスエイ・エルエルシー α−１，３−グルカングラフトコポリマー
JP2021514681A (ja) * 2018-03-09 2021-06-17 ニュートリション・アンド・バイオサイエンシーズ・ユーエスエー・フォー，インコーポレイテッド 遺伝子操作されたグルコシルトランスフェラーゼ
CN113574074B (zh) 2018-10-25 2023-03-21 营养与生物科学美国第四公司 α-1,3-葡聚糖接枝共聚物
WO2020131711A1 (en) 2018-12-17 2020-06-25 Dupont Industrial Biosciences Usa, Llc Polysaccharide derivatives and compositions comprising same
US20220267745A1 (en) * 2019-07-09 2022-08-25 Nutrition & Biosciences USA 4, Inc. Engineered alpha-1,3 branching enzymes
EP4013241A1 (en) 2019-08-16 2022-06-22 Nutrition & Biosciences USA 4, Inc. Flour- and meal-based food products comprising insoluble alpha-1,3-glucan
MX2022005441A (es) 2019-11-06 2022-10-10 Nutrition & Biosciences Usa 4 Inc Alfa-1,3-glucano altamente cristalino.
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EP4100446A1 (en) 2020-02-04 2022-12-14 Nutrition & Biosciences USA 4, Inc. Aqueous dispersions of insoluble alpha-glucan comprising alpha-1,3 glycosidic linkages
CN116134054A (zh) 2020-06-04 2023-05-16 营养与生物科学美国4公司 右旋糖酐-α-葡聚糖接枝共聚物及其衍生物
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CN117337308A (zh) 2021-05-04 2024-01-02 营养与生物科学美国4公司 包含氧化的不溶性α-葡聚糖的组合物
EP4334363A1 (en) 2021-05-04 2024-03-13 Nutrition & Biosciences USA 4, Inc. Compositions comprising insoluble alpha-glucan
EP4370560A1 (en) 2021-07-13 2024-05-22 Nutrition & Biosciences USA 4, Inc. Cationic glucan ester derivatives
WO2023055902A1 (en) 2021-09-30 2023-04-06 Dupont Nutrition Biosciences Aps Method for reducing sugar in food stuff
EP4426362A1 (en) 2021-11-05 2024-09-11 Nutrition & Biosciences USA 4, Inc. Glucan derivatives for microbial control
WO2023114942A1 (en) 2021-12-16 2023-06-22 Nutrition & Biosciences USA 4, Inc. Compositions comprising cationic alpha-glucan ethers in aqueous polar organic solvents
EP4463519A1 (en) 2022-01-12 2024-11-20 Nutrition & Biosciences USA 4, Inc. Coating compositions comprising rubber and insoluble alpha-glucan
EP4496834A1 (en) 2022-03-21 2025-01-29 Nutrition & Biosciences USA 4, Inc. Compositions comprising insoluble alpha-glucan
WO2024015769A1 (en) 2022-07-11 2024-01-18 Nutrition & Biosciences USA 4, Inc. Amphiphilic glucan ester derivatives
WO2024081773A1 (en) 2022-10-14 2024-04-18 Nutrition & Biosciences USA 4, Inc. Compositions comprising water, cationic alpha-1,6-glucan ether and organic solvent
EP4604740A1 (en) 2022-10-17 2025-08-27 International N&H Denmark ApS Method for improving flavor in plant-based food stuff
EP4611720A1 (en) 2022-11-02 2025-09-10 Nutrition & Biosciences USA 4, Inc. Compositions comprising xanthan gum and crystalline alpha-1,3-glucan
WO2024112740A1 (en) 2022-11-23 2024-05-30 Nutrition & Biosciences USA 4, Inc. Hygienic treatment of surfaces with compositions comprising hydrophobically modified alpha-glucan derivative
WO2024129953A1 (en) 2022-12-16 2024-06-20 Nutrition & Biosciences USA 4, Inc. Esterification of alpha-glucan comprising alpha-1,6 glycosidic linkages
AU2024242918A1 (en) 2023-03-29 2025-09-18 Danisco Us Inc. Methods for modifying texture in foodstuff via preferably in situ produced alpha-glucan
WO2024228945A1 (en) 2023-05-03 2024-11-07 Nutrition & Biosciences USA 4, Inc. Controlling bacterial cell adhesion with alpha-glucan comprising alpha-1,6 glycosidic linkages
WO2025006691A2 (en) 2023-06-30 2025-01-02 Nutrition & Biosciences USA 4, Inc. Porous alpha-1,3-glucan compositions
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Publication number Publication date
US20170002336A1 (en) 2017-01-05
US20190112585A1 (en) 2019-04-18
US20180305672A1 (en) 2018-10-25
US10633638B2 (en) 2020-04-28
CN107922958A (zh) 2018-04-17
US9988610B2 (en) 2018-06-05
AU2016280700A1 (en) 2018-01-04
US20170002335A1 (en) 2017-01-05
JP2018518179A (ja) 2018-07-12
CA2989337A1 (en) 2016-12-22
WO2016205391A1 (en) 2016-12-22
EP3310923A1 (en) 2018-04-25
EP3310906A1 (en) 2018-04-25
EP3310906B1 (en) 2020-05-06
WO2016205401A1 (en) 2016-12-22
US10508268B2 (en) 2019-12-17

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