AU2016271607C1 - Cell-controlled perfusion in continuous culture - Google Patents

Cell-controlled perfusion in continuous culture Download PDF

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Publication number
AU2016271607C1
AU2016271607C1 AU2016271607A AU2016271607A AU2016271607C1 AU 2016271607 C1 AU2016271607 C1 AU 2016271607C1 AU 2016271607 A AU2016271607 A AU 2016271607A AU 2016271607 A AU2016271607 A AU 2016271607A AU 2016271607 C1 AU2016271607 C1 AU 2016271607C1
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Australia
Prior art keywords
perfusion
culture
lactate
culture process
medium
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AU2016271607A1 (en
AU2016271607B2 (en
Inventor
Matthew Paul GAGNON
Gregory Walter Hiller
Ana Maria OVALLE
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Boehringer Ingelheim International GmbH
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Boehringer Ingelheim International GmbH
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Priority to AU2022259788A priority Critical patent/AU2022259788A1/en
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/0062—General methods for three-dimensional culture
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M29/00—Means for introduction, extraction or recirculation of materials, e.g. pumps
    • C12M29/10—Perfusion
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M27/00—Means for mixing, agitating or circulating fluids in the vessel
    • C12M27/02—Stirrer or mobile mixing elements
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
    • C12M41/26—Means for regulation, monitoring, measurement or control, e.g. flow regulation of pH
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
    • C12M41/30—Means for regulation, monitoring, measurement or control, e.g. flow regulation of concentration
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/0018—Culture media for cell or tissue culture
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2500/00—Specific components of cell culture medium
    • C12N2500/60—Buffer, e.g. pH regulation, osmotic pressure
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2510/00—Genetically modified cells
    • C12N2510/02—Cells for production
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2511/00—Cells for large scale production

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  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • Genetics & Genomics (AREA)
  • General Health & Medical Sciences (AREA)
  • General Engineering & Computer Science (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • Sustainable Development (AREA)
  • Cell Biology (AREA)
  • Analytical Chemistry (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Peptides Or Proteins (AREA)
AU2016271607A 2015-05-29 2016-05-27 Cell-controlled perfusion in continuous culture Active AU2016271607C1 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
AU2022259788A AU2022259788A1 (en) 2015-05-29 2022-10-27 Cell-controlled perfusion in continuous culture

Applications Claiming Priority (7)

Application Number Priority Date Filing Date Title
US201562168297P 2015-05-29 2015-05-29
US62/168,297 2015-05-29
US201562199388P 2015-07-31 2015-07-31
US62/199,388 2015-07-31
US201562246774P 2015-10-27 2015-10-27
US62/246,774 2015-10-27
PCT/US2016/034570 WO2016196261A1 (en) 2015-05-29 2016-05-27 Cell-controlled perfusion in continuous culture

Related Child Applications (1)

Application Number Title Priority Date Filing Date
AU2022259788A Division AU2022259788A1 (en) 2015-05-29 2022-10-27 Cell-controlled perfusion in continuous culture

Publications (3)

Publication Number Publication Date
AU2016271607A1 AU2016271607A1 (en) 2017-11-30
AU2016271607B2 AU2016271607B2 (en) 2022-07-28
AU2016271607C1 true AU2016271607C1 (en) 2023-06-29

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AU2022259788A Abandoned AU2022259788A1 (en) 2015-05-29 2022-10-27 Cell-controlled perfusion in continuous culture

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AU2022259788A Abandoned AU2022259788A1 (en) 2015-05-29 2022-10-27 Cell-controlled perfusion in continuous culture

Country Status (8)

Country Link
US (3) US11597903B2 (cg-RX-API-DMAC10.html)
EP (2) EP4148121A3 (cg-RX-API-DMAC10.html)
JP (2) JP7340324B2 (cg-RX-API-DMAC10.html)
KR (2) KR102658955B1 (cg-RX-API-DMAC10.html)
CN (1) CN107922919A (cg-RX-API-DMAC10.html)
AU (2) AU2016271607C1 (cg-RX-API-DMAC10.html)
CA (1) CA2985702A1 (cg-RX-API-DMAC10.html)
WO (1) WO2016196261A1 (cg-RX-API-DMAC10.html)

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US10704021B2 (en) 2012-03-15 2020-07-07 Flodesign Sonics, Inc. Acoustic perfusion devices
US10967298B2 (en) 2012-03-15 2021-04-06 Flodesign Sonics, Inc. Driver and control for variable impedence load
US9950282B2 (en) 2012-03-15 2018-04-24 Flodesign Sonics, Inc. Electronic configuration and control for acoustic standing wave generation
CN105939767B (zh) 2014-01-08 2018-04-06 弗洛设计声能学公司 具有双声电泳腔的声电泳装置
US11708572B2 (en) 2015-04-29 2023-07-25 Flodesign Sonics, Inc. Acoustic cell separation techniques and processes
US11021699B2 (en) 2015-04-29 2021-06-01 FioDesign Sonics, Inc. Separation using angled acoustic waves
US11377651B2 (en) 2016-10-19 2022-07-05 Flodesign Sonics, Inc. Cell therapy processes utilizing acoustophoresis
US11474085B2 (en) 2015-07-28 2022-10-18 Flodesign Sonics, Inc. Expanded bed affinity selection
US11459540B2 (en) 2015-07-28 2022-10-04 Flodesign Sonics, Inc. Expanded bed affinity selection
SG11201806310TA (en) 2016-01-26 2018-08-30 Boehringer Ingelheim Int Linked perfusion to continuous-flow stirred-tank reactor cell culture system
US11214789B2 (en) 2016-05-03 2022-01-04 Flodesign Sonics, Inc. Concentration and washing of particles with acoustics
US11085035B2 (en) 2016-05-03 2021-08-10 Flodesign Sonics, Inc. Therapeutic cell washing, concentration, and separation utilizing acoustophoresis
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WO2019071076A1 (en) 2017-10-06 2019-04-11 Lonza Ltd. AUTOMATED CONTROL OF CELL CULTURE BY SPECTROSCOPY RAMAN
HUE058924T2 (hu) * 2017-10-16 2022-09-28 Regeneron Pharma Perfúziós eljárások
CN114900773A (zh) 2017-12-14 2022-08-12 弗洛设计声能学公司 声泳系统及其操作方法、控制声换能器及声学系统的方法
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WO2020086408A1 (en) 2018-10-26 2020-04-30 The United States Of America, As Represented By The Secretary, Department Of Health And Human Services A high-yield perfusion-based transient gene expression bioprocess
CN110117527A (zh) * 2019-05-15 2019-08-13 刘宝全 一种干细胞代谢废物的强化排出方法
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Also Published As

Publication number Publication date
KR20180010202A (ko) 2018-01-30
KR20240055117A (ko) 2024-04-26
US11597903B2 (en) 2023-03-07
EP3303558A1 (en) 2018-04-11
JP2022050540A (ja) 2022-03-30
CA2985702A1 (en) 2016-12-08
JP7340324B2 (ja) 2023-09-07
US20180171279A1 (en) 2018-06-21
JP2018516079A (ja) 2018-06-21
EP3303558B1 (en) 2022-05-25
AU2022259788A1 (en) 2022-12-01
WO2016196261A1 (en) 2016-12-08
AU2016271607A1 (en) 2017-11-30
JP7579289B2 (ja) 2024-11-07
EP4148121A2 (en) 2023-03-15
US12173267B2 (en) 2024-12-24
EP4148121A3 (en) 2023-06-07
AU2016271607B2 (en) 2022-07-28
US20230242857A1 (en) 2023-08-03
KR102658955B1 (ko) 2024-04-22
CN107922919A (zh) 2018-04-17
US20250059486A1 (en) 2025-02-20

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Free format text: THE NATURE OF THE AMENDMENT IS AS SHOWN IN THE STATEMENT(S) FILED 15 FEB 2023

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