AU2016102398A4 - Method for enriching target nucleic acid sequence from nucleic acid sample - Google Patents

Method for enriching target nucleic acid sequence from nucleic acid sample Download PDF

Info

Publication number
AU2016102398A4
AU2016102398A4 AU2016102398A AU2016102398A AU2016102398A4 AU 2016102398 A4 AU2016102398 A4 AU 2016102398A4 AU 2016102398 A AU2016102398 A AU 2016102398A AU 2016102398 A AU2016102398 A AU 2016102398A AU 2016102398 A4 AU2016102398 A4 AU 2016102398A4
Authority
AU
Australia
Prior art keywords
nucleic acid
bait
sequences
sequence
target
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Active
Application number
AU2016102398A
Other languages
English (en)
Inventor
Wanshi CAI
Xingyi HANG
Wubin QU
Ruichao WANG
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Igenetech Biotech (beijing) Co Ltd
Original Assignee
Igenetech Biotech Beijing Co Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Igenetech Biotech Beijing Co Ltd filed Critical Igenetech Biotech Beijing Co Ltd
Application granted granted Critical
Publication of AU2016102398A4 publication Critical patent/AU2016102398A4/en
Active legal-status Critical Current
Anticipated expiration legal-status Critical

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6806—Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Zoology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Analytical Chemistry (AREA)
  • Microbiology (AREA)
  • Physics & Mathematics (AREA)
  • Molecular Biology (AREA)
  • Immunology (AREA)
  • Biotechnology (AREA)
  • Biophysics (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Chemical Kinetics & Catalysis (AREA)
AU2016102398A 2016-04-22 2016-11-21 Method for enriching target nucleic acid sequence from nucleic acid sample Active AU2016102398A4 (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
CN201610250133.3A CN105925671B (zh) 2016-04-22 2016-04-22 一种从核酸样品富集目标序列核酸的方法
CN201610250133.3 2016-04-22

Publications (1)

Publication Number Publication Date
AU2016102398A4 true AU2016102398A4 (en) 2019-05-02

Family

ID=56839769

Family Applications (2)

Application Number Title Priority Date Filing Date
AU2016102398A Active AU2016102398A4 (en) 2016-04-22 2016-11-21 Method for enriching target nucleic acid sequence from nucleic acid sample
AU2016403554A Pending AU2016403554A1 (en) 2016-04-22 2016-11-21 Method for enriching target nucleic acid sequence from nucleic acid sample

Family Applications After (1)

Application Number Title Priority Date Filing Date
AU2016403554A Pending AU2016403554A1 (en) 2016-04-22 2016-11-21 Method for enriching target nucleic acid sequence from nucleic acid sample

Country Status (3)

Country Link
CN (1) CN105925671B (zh)
AU (2) AU2016102398A4 (zh)
WO (1) WO2017181670A1 (zh)

Families Citing this family (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN105925671B (zh) * 2016-04-22 2019-07-23 艾吉泰康(嘉兴)生物科技有限公司 一种从核酸样品富集目标序列核酸的方法
CN106676169B (zh) * 2016-11-15 2021-01-12 上海派森诺医学检验所有限公司 一种用于乳腺癌易感基因brca1和brca2突变检测的杂交捕获试剂盒及其方法
CN108546739A (zh) * 2018-04-20 2018-09-18 曹顺 一种用于ngs测序的核酸目标序列富集的方法
CN111723261B (zh) * 2019-03-22 2021-08-13 昆明逆火科技股份有限公司 基于搜索引擎的dna比对算法
CN110343756B (zh) * 2019-06-25 2023-02-24 广西识远医学检验实验室有限公司 一组用于检测地中海贫血的探针及相关试剂盒和应用
EP4087943A1 (en) * 2020-03-26 2022-11-16 Integrated DNA Technologies, Inc. Hybridization capture methods and compositions

Family Cites Families (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2003093509A1 (en) * 2002-05-01 2003-11-13 Seegene, Inc. Methods and compositions for improving specificity of pcr amplication
US8192937B2 (en) * 2004-04-07 2012-06-05 Exiqon A/S Methods for quantification of microRNAs and small interfering RNAs
CN103602658A (zh) * 2013-10-15 2014-02-26 东南大学 一种新型靶向核酸分子的捕获与富集技术
CN105925671B (zh) * 2016-04-22 2019-07-23 艾吉泰康(嘉兴)生物科技有限公司 一种从核酸样品富集目标序列核酸的方法

Also Published As

Publication number Publication date
CN105925671B (zh) 2019-07-23
CN105925671A (zh) 2016-09-07
AU2016403554A1 (en) 2018-12-13
WO2017181670A1 (zh) 2017-10-26

Similar Documents

Publication Publication Date Title
AU2016102398A4 (en) Method for enriching target nucleic acid sequence from nucleic acid sample
EP3635136B1 (en) Single cell whole genome libraries for methylation sequencing
CA2810931C (en) Direct capture, amplification and sequencing of target dna using immobilized primers
US8986958B2 (en) Methods for generating target specific probes for solution based capture
JP6925424B2 (ja) 短いｄｎａ断片を連結することによる一分子シーケンスのスループットを増加する方法
US20150126377A1 (en) Selection of nucleic acids by solution hybridization to oligonucleotide baits
EP3377625A1 (en) Method for controlled dna fragmentation
AU2016102399A4 (en) Primer set for amplifying multiple target DNA sequences in sample and use thereof
JP7033602B2 (ja) ロングレンジ配列決定のためのバーコードを付けられたｄｎａ
US11401543B2 (en) Methods and compositions for improving removal of ribosomal RNA from biological samples
JP2006523465A5 (zh)
US20230056763A1 (en) Methods of targeted sequencing
JP2022516821A (ja) 複合体が表面結合されたトランスポソーム複合体
CN114729349A (zh) 条码化核酸用于检测和测序的方法
WO2020132316A2 (en) Target enrichment
CN106191256B (zh) 一种针对目标区域进行dna甲基化测序的方法
US20150024959A1 (en) METHOD FOR AMPLIFYING cDNA DERIVED FROM TRACE AMOUNT OF SAMPLE
CN111801428B (zh) 一种获得单细胞mRNA序列的方法
CN108103052B (zh) 提高基因组覆盖度的单细胞全基因组扩增及文库构建方法
EP4127220B1 (en) Methods and compositions for preparing nucleic acid libraries
US9315807B1 (en) Genome selection and conversion method
CN110546275A (zh) 用于去除不需要的核酸的方法和试剂盒
US20220136042A1 (en) Improved nucleic acid target enrichment and related methods
CN117089597A (zh) 一种单细胞文库构建测序方法及其应用

Legal Events

Date Code Title Description
FGI Letters patent sealed or granted (innovation patent)