AU2015376023A1 - Effective tilling promoter and effective tilling promoting method - Google Patents

Effective tilling promoter and effective tilling promoting method Download PDF

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AU2015376023A1
AU2015376023A1 AU2015376023A AU2015376023A AU2015376023A1 AU 2015376023 A1 AU2015376023 A1 AU 2015376023A1 AU 2015376023 A AU2015376023 A AU 2015376023A AU 2015376023 A AU2015376023 A AU 2015376023A AU 2015376023 A1 AU2015376023 A1 AU 2015376023A1
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yeast
productive
superheated steam
tillering
rice
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AU2015376023B2 (en
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Takashi Hamasaki
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Asahi Group Holdings Ltd
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Asahi Group Holdings Ltd
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    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01NPRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N63/00Biocides, pest repellants or attractants, or plant growth regulators containing microorganisms, viruses, microbial fungi, animals or substances produced by, or obtained from, microorganisms, viruses, microbial fungi or animals, e.g. enzymes or fermentates
    • A01N63/30Microbial fungi; Substances produced thereby or obtained therefrom
    • A01N63/32Yeast
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01GHORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
    • A01G7/00Botany in general
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01GHORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
    • A01G7/00Botany in general
    • A01G7/06Treatment of growing trees or plants, e.g. for preventing decay of wood, for tingeing flowers or wood, for prolonging the life of plants
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01NPRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N25/00Biocides, pest repellants or attractants, or plant growth regulators, characterised by their forms, or by their non-active ingredients or by their methods of application, e.g. seed treatment or sequential application; Substances for reducing the noxious effect of the active ingredients to organisms other than pests
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01NPRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N59/00Biocides, pest repellants or attractants, or plant growth regulators containing elements or inorganic compounds
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01NPRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N59/00Biocides, pest repellants or attractants, or plant growth regulators containing elements or inorganic compounds
    • A01N59/26Phosphorus; Compounds thereof

Abstract

[Problem] To provide novel technology by which effective tilling can be promoted. [Solution] Provided is an effective tilling promoter which comprises the product of a super-heated steam treatment of microbial materials, such product obtained by subjecting a mixture comprising yeast, yeast extract or yeast cell walls, phosphoric acid or a phosphoric acid compound, and potassium or a potassium compound to a super-heated steam treatment.

Description

DESCRIPTION
PRODUCTIVE TILLERING-PROMOTING AGENT AND METHOD OF PROMOTING
PRODUCTIVE TILLERING
Technical Field iiiiiiit...................... :I!!!!!!!!lll|. p<§rii.re|aii|||i|l a productive tlillillllprilillllillillit 1 Vilailillillii to treat [fice etc. ll§||be: :|||i:l§;:|pbmol|;:;^ ΐ|||1e b y ί n c r easi ng c r op y 1|11.. illilifround J|||||||||||||||||||||| [0002] A flillli c e s e e d I ί n g s t lllilip 1 a n iilp, V|i|liili vel|i owtillli s i;i :p:fiillll|||iid enters the tiileii;l|f' sta<|$| iilillil] neiilliberas grow illllilllliots of the see d|in11, lien repHductive growth proceeds, which includes, in the following order, the young ear formation stage, the booting stage, the heading and flowering stage, and the ripening stage, and ears of rice ripen and can be harvested.
The development of stems in the tillering stage is classified into productive tillJlll;l arid non-productive tiHer:ing. Among tie:! ililllli.......d|iildllilllliiiiiproducti|l||lii||fers aare:::::::ig:]t ilillllllliilllllllllllb fO:ii||ail Ifllllseeds, lllle non-productive iriiiiilllllllllllllllat |||lhdl ||llfiii|i§s aftefllheir development or form ears ν|1|||ϊΙο seeds.
As the number of!|idd||l|i^iii tilillis increases, the nu||)|llot ilil|ll|iil|!l:ant incri|i|es ||f i|li|forl|||fche pri|uctl|e tillelf lil: one:::biii||l|||!||iis t^||i|ay|p large inf Iflhce on|i|cr#a®e and dlllealJ irilblcip l|:Iel:|:|| [0003]
In. order to promote productive tillering to increase crop yield, attempts have been made to use fertilizers and. growth regulators such as plant hormones, but the effects of the use of these agents are ihdtlliifficilllt ί :0004 ] 111' A reducing fertilizer produced using a brewer's yeast etc. is known (for example, Patent Literature 1}. This reducing fertilizer is configured, for example, in a form containing a superheated steam-treated microbial material, and the superheated steam-treated microbial material can be obtained by, for example, subjecting a mixture containing phosphoric acid, potassium, and a yeast, an extract of the yeast, or a ceil wall of the yeast to superheated steam treatment (hydrothermal reaction treatment). Patent Literature 1 describes that the use of the reducing fertilizer of thilillts of fruit hiprgernent d|j f:|j||ts .
Citation List.
Pl||nt|||||:||tut|ll [!!§5j!!!!!!!!!!!!!!!!!| :ij|htelilliitei|j:|ture W02013 /:0::9 4 Ξ 3 :::Suiri|al|? of Ihiintilin l|pcih|i|al Piiliemiii 111 oil]”"
An lijeillllliliiie presehllll§§&amp;nti|h '|| to' prfi|||:Ie| | |lovel technique||fcapiii§||iii|i|3romotiri;|l:;|||iduct|;ye |liller||l|j-:,
Solution |lb Blllillilll! jllllli.......111! ||||||||||||^:|i:<^!lprn.tox" has|lli:nductei||exi§:|l|lbe J|lldle§l||bn a |lil:li|:lil|:cabllI|lll|lllitomoting p:roductib||ltiii||lilngl|||||iS: :ia Itbsttiil isent investor has lliund that||||jfe |hfmb|p:;btl! productive tliiiiilibiblbe increased by trailing rice etc. with the superheated steam-treated microbial material disclosed in Patent Literature I. The above-described effect of promoting productive tillering is not disclosed in Patent Literature 1. r a nadi
[ U UVjQ.J
The present invention is summarized as follows.
[1] A productive tillering-promoting agent containing a superheated steam-treated microbial material obtained by subjecting a mixture containing a yeast, an extract of a yeast or a cell wall of a yeast, phosphoric acid or a phosphoric acid compound, and potassium or a potassium compound to superheated steam treatment, [2] A method of promoting productive tillering, including treating a gramineous plant with a superheated steam-treated microbial material obtained by subjecting a mixture containing a yeast, an extract of a yeast or a cell wall of a yeast, phosphoric acid or a phosphoric acid compound, and potassium or a potassium compound to superheated steam treatment.
[3] The method of promoting productive tillering according to [2] , wherein the gramineous plant is treated by foliar application B of the superheated steam-treated microbial material, [4] The method of promoting productive tillering according to [2] or [3] , wherein the gramineous plant is rice, wheat, barley, rye or oat.
[5] The method of promoting productive tillering according 10 to any one of [2] to [4], wherein the gramineous plant is treated with the superheated steam-treated microbial material in a tillering stage .
[6] The method of promoting productive tillering according to [5] , wherein the gramineous plant is treated with the superheated 15 steam-treated microbial material in a productive tillering stage.
[7] The method of promoting productive tillering according to any one of [2] to [6], wherein the gramineous plant is paddy rice, and the method comprises treating the paddy rice cultivated and planted at 10,000 to 23, 000 plants per 10a with the superheated 20 steam-treated microbial material in an amount of 10 ml to 1,000 ml per 10 a.
Advantageous Effects of Invention [0009] 25
According to the present invention, a novel technique capable of promoting productive tillering can be provided.
Brief Description of Drawings iiiiiiiiiii FIG. 1 is a graph in Test Example 2 showing a crop yield in a section treated with a productive tillering-promoting agent in a|||ilimpl||||nd||iii c|i|p yield Ilian untreated......diction, iiiiiiii FIG|||||||:l|i| photograph :|i|:Tesl;|Example 3 showing an exampde of plant|!!illii:|iatei! in a s.ibhllbn. .bleated with lii|i producti ve tillering-promoting agent in the Example and. an example of plants cultiylllllliillii: urflt eated||lihi|on||| liiiilihhie qjt||me nt |!!!!!!!||| !|l!ll|!!l!l!ll!l
One embodiment of the present invention will be described in detail. A productive tillering-promoting agent in this embodiment is configured to contain a superheated steam-treatedmicrohial material. The superheated steam-treated microbial material can be obtained by subjecting a mixture containing a yeast, an extract of a yeast or a cell wall of a yeast, phosphoric acid or a phosphoric acid compound, and potassium or a potassium compound to superheated steam treatment (hydrothermal reaction treatment).
[0012]
The superheated steam-treated microbial material is disclosed in Patent Literature 1 above etc. and can be produced, for example. using known materials according to a method disclosed in Patent lllllll th|;l|lliient degi;lll|i|:io|:|lllhe superheated stea|||lli|:tment |hy|il|i|ierntll|||idtion ||lii||ien|i||l^ a method in|l:uding|||||lhtir:g |upl|i||tedlllili: by hea|li|land||||ssuri2ation i|| chan|l||i||ical i|ro||i||:es dilllfobject by the i:||§:§ence of the ||perhe|||i|||ili::, iihe ili|l:ratull is preferably ||:||§ or higher a|| 220β|||ί|||ΐ|||ί||ί: ;:|hd radlilif eferably 150° or high|iil||'id 210°C or loilr . The pressure 11 preferably 0.9 MPa or high#il||id 1.9 MPa o||||ower, and more l|lferaB||l|. 2 MP:||1|;. higher 1|1||| 8 MPa or low:|§| Particularly, tli hydroiiiilmailiiiiillipn tre||i|ilt performed alii pressure of............ i:M|i|or hig:iiii^ii!!i|Sii^!i!ii!iiiii!ij^i^ and a:ll:l|:pa lllhigher and 2:201(3: (oillliiwer itilition treatment......... :::pe|li::||ted atllllllilllllllllllllllllld 1.9|||||||||r|||li|:er and at 50°C or higher and 21Q°C or lower is more preferred. The hydrothermal reaction treatment performed at 1.2 MPa or higher and 1.8 MPa or lower and at 150° or higher and 210°C or lower is still more preferred.
No particular limitation is imposed on the yeast, the extract of the yeast, and the cell wall of the yeast. They may be derived from at least one selected from the group consisting of brewer's yeast slurries, pressed brewer's yeasts, dry brewer's yeasts, brewer's yeast suspensions, dry yeast cell walls, yeast cell wall suspensions and brewer's yeast-containing inorganic materials.
Phosphoric acid or a phosphoric acid compound may be used alone, or a mixture of two or more types thereof may be used. The phosphoric acid compound used may be any phosphoric acid compound known as citric acid-soluble fertilizers may be used, and examples of such fertilizers may include: calcium superphosphate obtained by treating rock phosphate with sulfuric acid to solubilize phosphoric acid; triple superphosphate; and fused phosphate fertilizers in the form of a mixture; and calcined phosphate fertilizers in. the form of a mixture. ^lllllllli141111mbiiiilassiumcoi|oand!|1||||sedi||iiamiiififife;i potassium compound compoundlnown z l|:::|||erti l|i|eh, 'lid spieifie lude potassium c hloride, pit a is i ilii u Jiiii'e| iliilfitoxideiiitni: i potassium hit rate . lilltwblbrMdiilltvliillllhereof may......be used, exancles t ''liill|:lii:iculi;r:||imitation is imposed on the mi rifle.......dil the yeast, the extract of the yeast or the cell wall of the yeast, phosphoric acid or the phosphoric acid compound, and potassium or the potassium compound, and the person skilled in the art can be appropriately set the mixing ratio. For example, more than 0 parts by weight and 135 parts by weight or less of phosphoric acid or the phosphoric acid compound and more than 0 parts by weight and 100 parts by weight or less of potassium or the potassium compound can be used relative to 100 parts by weight the yeast, the extract of the yeast, or the cell wall of the yeast.
[0013]
Mo particular iim.itat.ion is imposed on the usage of the produeti villi Her ing-prbtHf If :|ii: ageiillllil thi ilpmb<|dif#η |. !| F§»r l|i example, a gramineous printlllch may :f|§ tlieiitiid: a|| |;ρη||: once with the productive t.iliering-promoting agent in this embodiment before the tillering stage or during the tillering stage (when the number of true leaves is 6 to 10). The tillering stage includes a productive tillering stage (when the number of true leaves S is 6 to 8) and a non-productive tillering stage (when the number of true leaves is 9 to 10) . It is preferable to perform the treatment with the productive tiliering-promoting agent in this embodiment in the productive tillering stage, because the number of productive tillers can be further increased to thereby increase the crop yield-10 No particular limitation is imposed on the method of the treatment. From the viewpoint of the efficiency of farm work, it is preferable to treat gramineous plants by foliar application. .............For„..pa.ddyIIice.,:....it.^|l™bre tliilllliil .........:::::::p:rpductivl::::fillerihiil|^ijii^iin^!igenl!i|!|(iiiid into a paddy s 1 u : ce...... IP"..... ..............paillilllr liMtati|f|: is i||||l|l|||ilfhe amount for flip illlliiteatmehffs......For example, whii||foliarl:i|:|liilli:ldn is used, tl||||| prdiiltiye......h|§ifl|ri|||l|i§|i|f agent i|||||i||||solution) in||l|| :i!!!!!!!^|^iod;i:ad in an ai||ll|||||lhin the range of; ......ΙΟ milder 10 a at a Jilutiohiili^ 1 to ΙΟί^ΟΙίΟν;:
Ill.............................................. solutililis admihi|li|ed in an amount:: ......::::s:|:it(||:n the rllgelbf illiiliiip 400|||||||t a dilut||§| factor ofssilssto:: !|||||ΐ;:;1Ι|ρ dillllllllllllf ':^ii|j|ii!iipp!irixrii^!ίϊίί|ίϊίίίϊ^|ίρ. ||:]|i|psed on the gramineous plant lllllllleited withlllheliilliuctivel|l|||li|i:|"promotin|||gent in this ...........|imibdiffiiht:>........lpd':it||:p;ll|p.n s3l||||| i|||be art ^a}|:|b approp|||le||| set the gramineous plant. Examples of the gramineous plant may include rice and wheat variety such as wheat, barley, rye, and oat. The rice may be any of paddy rice and upland rice. When paddy rice is used, the number of plants per 10 a is generally 10, 000 to 23,000. 5: [0014]
With the productive tillering-promoting agent in this embodiment, the number of productive tillers of the treated gramineous plant such as rice can be increased. Therefore, effects such as an increase in the number of ears per plant, improvement 10 of ripening (an increase in the number of fertile grains and a reduction in sterile rate) , and an increase in thousand-kernel weight Qillibe :ϊ||Ι1ί1|:|1:, sdithat the;.......crop yield can be increased. ..........When....a....grihlheiiillbiiiilliiih as flee is ibiabidiiith the
Sjlperhe.at ed atppillt rihied ini c rob i a 1|:ΐ||| e ri a 1 a c|g tblblllilll. !|t5· footingllfrdbflets) is promoted, andlllhillillbhjit of --¾ llllferliilizepliie increasesl||:|In additii|hii|illl|iits such llllas illedidilbn'in lodging can be obtained. Therefor|:|||hllii|p yield lllloflllhel|treated...grami:He:Guss plant such hi rice can lii||||h|i:lased. jilt [ B^:hrn^:i.€5^::|!!!!!!!!!!!!iii
Ilf Jl§lit !||l 11 Tie pppfippt inlii|i|in iiiillliillesiiibed more splilil|ili|ly by :!!l#a.yl;:pf low'dli:|o#t: liiiilipiiiientllinvention isl:i:iiill|||mitedl 1111' thifeib J!!"' l|||:0l|! j| ? '2 SJlll [ P J|lu| t IIP" o f "pll dplf i f hi iilillliiliil p t into ting agen tiiiiliiiiiliimp 1 ill A productive tillering-promoting agent containing a superheated steam-treated microbial material in an Example was produced as follo*|$§||||
First , a magh|||§if stirrin«||f||||e ion pot ii$§(S:| charged with 57 0 yeast c|il||iiali|||and |||::|:|||||i|;|:of a liquid fertilizer adjusted to contain 8% by weight of phosphoric acid and 7% by weight, of potassium to obtain a solution mixture. The solution mixture was heated under stirring to fill the reaction pot with steam. Then a lid was put on the reaction pot, and the reaction pot was heated at a temperature of 170°C and a pressure of 0.95 MPa to obtain 3,800 kg of a productive tiliering-promoting agent as a superheated steam-treated microbial material. !i|jllf!!!______________________________________________ |Tei!!: ifipi$ili: 1111111 III Tilree i|d|tl||ctions including (l)l|l|l|l|||spctioi:' in which a 1.3ll!dlutior| Ifilhe productive tiller:iii|i|i|||||i|ng agent in the lx!!!!# was s||a||d to paddy rice {t^|||||||i||||iljfice OM4 900) , j||| i| |ii:| Iscptiohl fii which a 0.3% sol|i|ih::::':'df Vipac 88 was sprayed to paddy rice (type: indica rice OM490G), and (3} a test section in which no promoting agent was sprayed to paddy rice (type: indica rice ©M490Q) were set. In each test section, foliar application in an amount of 40 L per 10 a was performed over the entire aerial parts of plants 28 days and 50 days after rice planting. The Vipac 88 (a-Naphthyi Acetic Acid (a-N.A.A), b-Naphthoxy Acetic Acid (β-Ν.Α.Α)) is a traditional plant growth regulator and is one type of auxin.
The day that is 28 days after the rice planting corresponds to the productive tillering stage, and the day that is 50 days after the rice planting corresponds to the booting stage.
The paddy rice treated as described above was cultivated. Ninety days after the rice planting, the crop yield in each of the test sections was estimated from a "one-tsubo" area. The results are shown in TABLb 1, [0018] 10 [TABLE 1] TOTAL CROP YIELD (t/ha) NUMBER OF EARS (EARS/ro2) THOUSAND -KERNEL WEIGHT (g> NUMBER OF RIPENED GRAINS (GRAINS ! /20 EARS) NUMBER OF GRAINS PER EAR 0F™° (GRAINS /20 EARS) I '*·’ DEGREE OF LODGING PRODUCTIVE TILLERING-PROMOTINQ AGENT 7.73 a 480 24.90 1746 2729 ] 63.38 2 VioaeSS 6.80 b 430 24.58 1349 ......... 2, .. .. UNTREATED 5.40 c 3:98 , 23J4 ......1039 2334 ]..., 48,51 .........1,,,,1 *The total crop yield was determined by repeating a crop yield survey three times.
Different letters, a, b, and c, indicate significant differences p < 0.05 *The degree of lodging was evaluated in the following five grades. I: hard trees, completely healthy 2: relatively healthy trees, most trees still standing 3: most trees leaning 4: weak trees, mostly fallen tree 5: very weak trees, all trees have fallen [0019]
As a result of the foliar application of the productive tillering promoting agent, the total crop yield increased (by a 15 factor of 1.43) , the number of ears increased (by a factor of 1.21) , the thousand-kernel weight increased (by a factor of 1.04), the number of ripened grains increased (by a factor of 1.68) , the number of grains per ear increased (by a factor of 1.22) , and the percentage 11 of iiflcreasedjijilbygiig |iE dbt^Opisof 1.38 ) Hi ::i|n aI:d|il|0J|idi iiot 3Pr0:iii:p±Ooi|ii!:diii^^^:,...fiiS't main||||i|i||| i|^ifei.x:^ilipbta.i.r^fecl!i!iiis.iiqiiiii^ib-iiiiiiiii the degree ||f i:|d||f ih|i|iii|i||i|dv||?^; (Jihi|||ii:^ii:|| :dii:i:i:i:ldl|illig Iras s3:):ii-i: |||| [od|ofll|||| :||les||: Exaif|ll||||j::h: tii| st siddit Ills incl:i|iing||( i| ^|ibsl|||:|i||ii|iin ihl§pich a 0.1% ::§oliJ|ioli(|f '||giiii|3:ifC?idillive t^i||i^er!;|g^!p3|i!ij^ot in the Example ;:iii|i:ii::.s|||ayl| !§ i>:gfi|p(§:|ce ||:ype:|| ji|pd||ca x:ice'iiil|M^^:MiM^iilid^iP^i (2} ...a,, tdif :;^iii#:c:lfi!:pii!iii^ri! ^iiililiiiliiiirto ϋ||:ϊτom^H ij^ig i|dnt was :|i|ii|i|'i8ti|i^
Hill, Itypi:* '::i|iil|)g|i|.li||i|ii^ |||ΕΝϋ||§} iiiiiwefiiiiiset. ....... foliar application of the productive ti'ilering-promoting agent in an amount of 200 L per 10 a (about 20,000 plants) was performed over the entire aerial parts of plants in the productive tillering stage(the number of true leaves: 6 to 10). Immediately before harvesting, the average number of ears per plant was examined by a standing crop survey. After the harvesting, the products were sent, to a rice center to examine the total crop yield, the ratio of abortive rice kernels (the amount of abortive rice kernels per 10 a) , and the ratio of first grade rice (the amount of first grade rice per 10 a) . The abortive rice kernels are kernels passing through a 1.8 to 2 mm sieve. The first grade rice is rice not passing through the above sieve and is classified, as first grade rice, which is the highest grade rice, in a grade examination performed on the basis of. t^isgdaifity of lowlancfliin-glptinillpiforowniitice.....and lowland g;t(ai 1η|§|$ i|i||)wn rice def ined by Itie iliiiitby|c|f lllgri cult are and . ||
The results are shown in FIG. 1.
[0021]
As can be seen from FIG. 1, as a result of the foliar application of the productive tillering-promoting agent, the.....total crop yield increased (oy :or of 1 .*!:!!.....liandlthe nu||f)ei|clf ears increased i;. |by;ia factdiii:iiii|||||||||:: ^illnlliDer o|s|bo:ii|ii|!i:ijeikfernels decreased,....... )ah:|llllllldunt':'sil' f ir|p::'vf:||de:|llde '::§ilg:r eased;,;;· so;; that the||lil|:|| was xmprgv ij |ί|0|:2 ] .sin c ldiih:§li[iil;a. test seiilf i diliilwhi;dligilQ::i;:l;ll solution of the productive tillering-promoting agent was sprayed fc© paddy rice (type: black rice SAYOMURASAKI) and (2) a test section in which no promoting agent was sprayed to paddy rice (type: black, rice SAYOMUPASAKI} were set. For the test section {!}, the foliar application of the productive tillering-promoting agent, in an amount of 500 L per 10 a (about 12,000 plants) was performed over the entire aerial parts of plants 30 days and 100 days after rice planting. The day that is 30 days after the rice planting corresponds to the productive tillering stage to the non-productive tillering stag- . and the day that is 100 days after the rice planting correspon · ·. to the heading stage. 25 [0023]
After harvesting f the roots were Ϊ if ted and examined. As shown FIG. 2, the amount of rooting (the amount of rootlets) and the her of tillers were large in the section (1) (treated section) . in ] nurr The; the pro state wa|;;;;;§|a±f ||.ined. In .......sepiiBn 111) g a;llllll|e;Sult oJlithe fo|;|;|i|)appl|i:|ti;§||| of the dg.ei!i%:tJilliing-pr.omot: tififii|||11||::11||;| crd|||iellilhcreaseds

Claims (6)

1. A productive tillering-promoting agent comprising a, superheated steam-treated microbial material obtained by subjecting a mixture containing a yeast, an extract of a yeast or a cell wall of a yeast, phosphoric acid or a phosphoric acid compound, and potassium or a potassium compound to superheated steam treatment.
2, A method of promoting productive tillering, comprising treating a gramineous plant with a superheated steam-treated microbial material obtained by subjecting a mixture containing a yeast, an extract of a yeast or a cell wail of a yeast, phosphoric acid or a phosphoric acid compound, and potassium or a potassium compound to superheated steam treatment.
3 . The method of promoting productive tillering according to claim 2, wherein the gramineous plant is treated by foliar application of the superheated steam-treated microbial material.
4 . The method of promoting productive tillering according to claim 2 or 3, wherein the gramineous plant is rice, wheat, barley, rye or oat.
5, The method of promoting productive tillering according to any one of claims 2 to 4, wherein the gramineous plant is treated with the superheated steam-treated microbial material in a tillering stage.
6. The method of promoting productive tillering according to claim 5, wherein the gramineous plant is treated with the superheated steam-treated microbial material in a productive tillering stage.
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