AU2015292311B2 - Methods of determining tissues and/or cell types giving rise to cell-free DNA, and methods of identifying a disease or disorder using same - Google Patents

Methods of determining tissues and/or cell types giving rise to cell-free DNA, and methods of identifying a disease or disorder using same Download PDF

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AU2015292311B2
AU2015292311B2 AU2015292311A AU2015292311A AU2015292311B2 AU 2015292311 B2 AU2015292311 B2 AU 2015292311B2 AU 2015292311 A AU2015292311 A AU 2015292311A AU 2015292311 A AU2015292311 A AU 2015292311A AU 2015292311 B2 AU2015292311 B2 AU 2015292311B2
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cfdna
cell
nucleosome
sample
genomic
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AU2015292311A1 (en
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Martin Kircher
Jay Shendure
Matthew Snyder
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University of Washington
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University of Washington
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    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
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AU2015292311A 2014-07-25 2015-07-27 Methods of determining tissues and/or cell types giving rise to cell-free DNA, and methods of identifying a disease or disorder using same Active AU2015292311B2 (en)

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US201462029178P 2014-07-25 2014-07-25
US62/029,178 2014-07-25
US201462087619P 2014-12-04 2014-12-04
US62/087,619 2014-12-04
PCT/US2015/042310 WO2016015058A2 (en) 2014-07-25 2015-07-27 Methods of determining tissues and/or cell types giving rise to cell-free dna, and methods of identifying a disease or disorder using same

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EP (2) EP3172341A4 (cg-RX-API-DMAC7.html)
JP (3) JP2017522908A (cg-RX-API-DMAC7.html)
KR (2) KR102696857B1 (cg-RX-API-DMAC7.html)
CN (2) CN107002122B (cg-RX-API-DMAC7.html)
AU (2) AU2015292311B2 (cg-RX-API-DMAC7.html)
CA (1) CA2956208C (cg-RX-API-DMAC7.html)
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Families Citing this family (119)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US9260753B2 (en) 2011-03-24 2016-02-16 President And Fellows Of Harvard College Single cell nucleic acid detection and analysis
US9892230B2 (en) 2012-03-08 2018-02-13 The Chinese University Of Hong Kong Size-based analysis of fetal or tumor DNA fraction in plasma
US11261494B2 (en) 2012-06-21 2022-03-01 The Chinese University Of Hong Kong Method of measuring a fractional concentration of tumor DNA
US11913065B2 (en) 2012-09-04 2024-02-27 Guardent Health, Inc. Systems and methods to detect rare mutations and copy number variation
US10876152B2 (en) 2012-09-04 2020-12-29 Guardant Health, Inc. Systems and methods to detect rare mutations and copy number variation
KR102393608B1 (ko) 2012-09-04 2022-05-03 가던트 헬쓰, 인크. 희귀 돌연변이 및 카피수 변이를 검출하기 위한 시스템 및 방법
US20160040229A1 (en) 2013-08-16 2016-02-11 Guardant Health, Inc. Systems and methods to detect rare mutations and copy number variation
SG11201604923XA (en) 2013-12-28 2016-07-28 Guardant Health Inc Methods and systems for detecting genetic variants
TWI813141B (zh) 2014-07-18 2023-08-21 香港中文大學 Ｄｎａ混合物中之組織甲基化模式分析
CN107002122B (zh) 2014-07-25 2023-09-19 华盛顿大学 确定导致无细胞dna的产生的组织和/或细胞类型的方法以及使用其鉴定疾病或紊乱的方法
US10364467B2 (en) 2015-01-13 2019-07-30 The Chinese University Of Hong Kong Using size and number aberrations in plasma DNA for detecting cancer
WO2016123698A1 (en) * 2015-02-06 2016-08-11 Uti Limited Partnership Diagnostic assay for post-transplant assessment of potential rejection of donor organs
CA2976303A1 (en) 2015-02-10 2016-08-18 The Chinese University Of Hong Kong Detecting mutations for cancer screening and fetal analysis
EP3325664B1 (en) 2015-07-23 2021-12-29 The Chinese University Of Hong Kong Analysis of fragmentation patterns of cell-free dna
EP4180532A1 (en) * 2015-11-09 2023-05-17 Roche Molecular Systems, Inc. Methods for determining the origin of dna molecules
CN108603228B (zh) 2015-12-17 2023-09-01 夸登特健康公司 通过分析无细胞dna确定肿瘤基因拷贝数的方法
US11514289B1 (en) 2016-03-09 2022-11-29 Freenome Holdings, Inc. Generating machine learning models using genetic data
EP4322168A3 (en) * 2016-07-06 2024-05-15 Guardant Health, Inc. Methods for fragmentome profiling of cell-free nucleic acids
JP7048105B2 (ja) 2016-07-15 2022-04-05 ザ リージェンツ オブ ザ ユニバーシティ オブ カリフォルニア 核酸ライブラリを生成する方法
WO2018027176A1 (en) 2016-08-05 2018-02-08 The Broad Institute, Inc. Methods for genome characterization
JP6560465B1 (ja) 2016-09-30 2019-08-21 ガーダント ヘルス， インコーポレイテッド 無細胞核酸の多重解像度分析のための方法
US9850523B1 (en) 2016-09-30 2017-12-26 Guardant Health, Inc. Methods for multi-resolution analysis of cell-free nucleic acids
US20200048711A1 (en) * 2016-10-12 2020-02-13 Bellwether Bio, Inc Determining cell type origin of circulating cell-free dna with molecular counting
MY205940A (en) 2016-10-19 2024-11-21 Univ Hong Kong Chinese Gestational age assessment by methylation and size profiling of maternal plasma dna
SG11201903509QA (en) 2016-10-24 2019-05-30 Univ Hong Kong Chinese Methods and systems for tumor detection
IL315032A (en) 2016-11-30 2024-10-01 Univ Hong Kong Chinese Analysis of cell-free dna in urine and other samples
WO2018112100A2 (en) 2016-12-13 2018-06-21 Bellwether Bio, Inc. Determining a physiological condition in an individual by analyzing cell-free dna fragment endpoints in a biological sample
EP3559270A2 (en) 2016-12-22 2019-10-30 Guardant Health, Inc. Methods and systems for analyzing nucleic acid molecules
CN110691792A (zh) 2017-01-10 2020-01-14 朱诺治疗学股份有限公司 细胞疗法的表观遗传学分析及相关方法
MY197535A (en) 2017-01-25 2023-06-21 Univ Hong Kong Chinese Diagnostic applications using nucleic acid fragments
WO2018210877A1 (en) 2017-05-15 2018-11-22 Katholieke Universiteit Leuven Method for analysing cell-free nucleic acids
EP3635134A4 (en) * 2017-06-09 2021-03-03 Bellwether Bio, Inc. DIAGNOSTIC CANCER OR OTHER PHYSIOLOGICAL CONDITIONS USING CIRCULATING NUCLEIC ACID FRAGMENT SENTINEL END POINTS
WO2018227202A1 (en) 2017-06-09 2018-12-13 Bellwether Bio, Inc. Determination of cancer type in a subject by probabilistic modeling of circulating nucleic acid fragment endpoints
US10636512B2 (en) 2017-07-14 2020-04-28 Cofactor Genomics, Inc. Immuno-oncology applications using next generation sequencing
ES2959360T3 (es) 2017-07-26 2024-02-23 Univ Hong Kong Chinese Mejora del cribado del cáncer mediante ácidos nucleicos víricos acelulares
AU2018348249A1 (en) * 2017-10-12 2020-04-16 Nantomics, Llc Cancer score for assessment and response prediction from biological fluids
CN107545153B (zh) * 2017-10-25 2021-06-11 桂林电子科技大学 一种基于卷积神经网络的核小体分类预测方法
WO2019024341A1 (zh) * 2017-11-27 2019-02-07 深圳华大生命科学研究院 一种体液游离dna的文库构建方法及其应用
CN108061794B (zh) * 2017-12-25 2020-03-27 苏州大学 一种无染色、无探针、无损检测细胞或类细胞结构微生物的类型及其周期的方法
JP7296969B2 (ja) 2018-01-12 2023-06-23 クラレット バイオサイエンス， エルエルシー 核酸を解析するための方法および組成物
WO2019175876A2 (en) * 2018-03-13 2019-09-19 Yissum Research Development Company Of The Hebrew University Of Jerusalem Ltd. Diagnostic use of cell free dna chromatin immunoprecipitation
CN112236520B (zh) 2018-04-02 2025-01-24 格里尔公司 甲基化标记和标靶甲基化探针板
WO2019209884A1 (en) * 2018-04-23 2019-10-31 Grail, Inc. Methods and systems for screening for conditions
AU2019269679B2 (en) * 2018-05-18 2025-10-30 The Johns Hopkins University Cell-free DNA for assessing and/or treating cancer
WO2019236478A1 (en) * 2018-06-04 2019-12-12 Guardant Health, Inc. Methods and systems for determining the cellular origin of cell-free nucleic acids
US11629345B2 (en) 2018-06-06 2023-04-18 The Regents Of The University Of California Methods of producing nucleic acid libraries and compositions and kits for practicing same
CN108796056A (zh) * 2018-06-28 2018-11-13 元码基因科技（北京）股份有限公司 基于游离dna的目标基因捕获技术进行组织溯源的方法
WO2020006369A1 (en) * 2018-06-29 2020-01-02 Guardant Health, Inc. Methods and systems for analysis of ctcf binding regions in cell-free dna
CN108913682A (zh) * 2018-07-05 2018-11-30 上海奥测医疗科技有限公司 一种制备cfDNA参考品的方法
CA3107359A1 (en) * 2018-07-23 2020-01-30 The Chinese University Of Hong Kong Cell-free dna damage analysis and its clinical applications
CN109448783B (zh) * 2018-08-07 2022-05-13 清华大学 一种染色质拓扑结构域边界的分析方法
CA3111887A1 (en) 2018-09-27 2020-04-02 Grail, Inc. Methylation markers and targeted methylation probe panel
SG11202100960RA (en) * 2018-10-08 2021-02-25 Freenome Holdings Inc Transcription factor profiling
CN111172263A (zh) * 2018-11-12 2020-05-19 北京医院 一种应用于无创产前检测的参考物质及其制备方法
CN113227468A (zh) * 2018-11-21 2021-08-06 卡里乌斯公司 感染性疾病的检测和预测
US20200199685A1 (en) 2018-12-17 2020-06-25 Guardant Health, Inc. Determination of a physiological condition with nucleic acid fragment endpoints
DK3899018T3 (da) * 2018-12-19 2024-01-08 Univ Hong Kong Chinese Cellefri DNA-endeegenskaber
US11657897B2 (en) * 2018-12-31 2023-05-23 Nvidia Corporation Denoising ATAC-seq data with deep learning
MX2020014095A (es) * 2019-01-24 2021-03-09 Illumina Inc Metodos y sistemas para monitorear la salud y enfermedad de organos.
CN113661249B (zh) 2019-01-31 2025-07-01 夸登特健康公司 用于分离无细胞dna的组合物和方法
CN109841265B (zh) * 2019-02-22 2021-09-21 清华大学 使用片段化模式确定血浆游离核酸分子组织来源的方法和系统及应用
WO2020198942A1 (zh) * 2019-03-29 2020-10-08 中国科学技术大学 基于峰聚类的单细胞染色质可及性测序数据分析方法和系统
WO2020243722A1 (en) 2019-05-31 2020-12-03 Guardant Health, Inc. Methods and systems for improving patient monitoring after surgery
CN110272985B (zh) * 2019-06-26 2021-08-17 广州市雄基生物信息技术有限公司 基于外周血血浆游离dna高通量测序技术的肿瘤筛查试剂盒及其系统与方法
CN114616343A (zh) 2019-09-30 2022-06-10 夸登特健康公司 用于在甲基化分区测定中分析无细胞dna的组合物和方法
CN110739027B (zh) * 2019-10-23 2023-04-18 深圳吉因加医学检验实验室 一种基于染色质区域覆盖深度的癌症组织定位方法及系统
CN114746560A (zh) 2019-11-26 2022-07-12 夸登特健康公司 改进甲基化多核苷酸结合的方法、组合物和系统
JP2023506560A (ja) * 2019-12-18 2023-02-16 ザ チャイニーズ ユニバーシティ オブ ホンコン 無細胞ｄｎａ断片化およびヌクレアーゼ
US20220389513A1 (en) * 2019-12-19 2022-12-08 Agency For Science, Technology And Research A Method of Estimating a Circulating Tumor DNA Burden and Related Kits and Methods
EP4077735A4 (en) * 2019-12-20 2024-01-24 AccuraGen Holdings Limited METHOD AND SYSTEMS FOR DETECTING DISEASES
US20230042332A1 (en) 2019-12-24 2023-02-09 Vib Vzw Disease Detection in Liquid Biopsies
AU2021205853A1 (en) * 2020-01-08 2023-11-23 Grail, Inc. Biterminal dna fragment types in cell-free samples and uses thereof
US20230053409A1 (en) * 2020-01-09 2023-02-23 Seoul National University R&Db Foundation Atac-seq data normalization and method for utilizing same
CN111254194B (zh) * 2020-01-13 2021-09-07 东南大学 基于cfDNA的测序及数据分析的癌症相关生物标记及其在cfDNA样品分类中的应用
WO2021163630A1 (en) * 2020-02-13 2021-08-19 10X Genomics, Inc. Systems and methods for joint interactive visualization of gene expression and dna chromatin accessibility
US11211147B2 (en) 2020-02-18 2021-12-28 Tempus Labs, Inc. Estimation of circulating tumor fraction using off-target reads of targeted-panel sequencing
US11211144B2 (en) 2020-02-18 2021-12-28 Tempus Labs, Inc. Methods and systems for refining copy number variation in a liquid biopsy assay
US11475981B2 (en) 2020-02-18 2022-10-18 Tempus Labs, Inc. Methods and systems for dynamic variant thresholding in a liquid biopsy assay
CN113362897A (zh) * 2020-03-06 2021-09-07 福建和瑞基因科技有限公司 基于核小体分布特征的肿瘤标志物筛选方法及应用
WO2021222828A1 (en) 2020-04-30 2021-11-04 Guardant Health, Inc. Methods for sequence determination using partitioned nucleic acids
AU2021276524A1 (en) * 2020-05-22 2023-01-05 Aqtual, Inc. Methods for characterizing cell-free nucleic acid fragments
CN111724860B (zh) * 2020-06-18 2021-03-16 深圳吉因加医学检验实验室 一种基于测序数据识别染色质开放区域的方法及装置
EP4407042A3 (en) 2020-07-10 2024-09-18 Guardant Health, Inc. Methods of detecting genomic rearrangements using cell free nucleic acids
WO2023282916A1 (en) 2021-07-09 2023-01-12 Guardant Health, Inc. Methods of detecting genomic rearrangements using cell free nucleic acids
CN111881418B (zh) * 2020-07-27 2023-05-16 中国农业科学院农业信息研究所 一种基于二分法的大豆气象产量预测方法及系统
EP4585697A3 (en) 2020-07-30 2025-08-06 Guardant Health, Inc. Methods for isolating cell-free dna
CN112085067B (zh) * 2020-08-17 2022-07-12 浙江大学 一种高通量筛选dna损伤反应抑制剂的方法
EP4205126A1 (en) 2020-08-25 2023-07-05 Guardant Health, Inc. Methods and systems for predicting an origin of a variant
US20230348997A1 (en) * 2020-09-17 2023-11-02 The Regents Of The University Of Colorado, A Body Corporate Signatures in cell-free dna to detect disease, track treatment response, and inform treatment decisions
JP2023544721A (ja) 2020-09-30 2023-10-25 ガーダント ヘルス， インコーポレイテッド 分配およびメチル化依存性ヌクレアーゼを使用してｄｎａを解析するための組成物および方法
US12234518B2 (en) 2020-10-23 2025-02-25 Guardant Health, Inc. Compositions and methods for analyzing DNA using partitioning and base conversion
WO2022115810A1 (en) 2020-11-30 2022-06-02 Guardant Health, Inc. Compositions and methods for enriching methylated polynucleotides
WO2022140629A1 (en) 2020-12-23 2022-06-30 Guardant Health, Inc. Methods and systems for analyzing methylated polynucleotides
TW202242145A (zh) * 2020-12-29 2022-11-01 比利時商比利時意志有限公司 核小體耗盡循環無細胞染色質片段之轉錄因子結合位點分析
WO2022173698A1 (en) * 2021-02-09 2022-08-18 Illumina, Inc. Methods and systems for monitoring organ health and disease
WO2022174109A1 (en) 2021-02-12 2022-08-18 Guardant Health, Inc. Methods and compositions for detecting nucleic acid variants
JP2024511425A (ja) 2021-03-25 2024-03-13 ガーダント ヘルス， インコーポレイテッド 免疫細胞ｄｎａを定量するための方法および組成物
CA3214391A1 (en) * 2021-04-08 2022-10-13 Gavin HA Cell-free dna sequence data analysis method to examine nucleosome protection and chromatin accessibility
CA3214321A1 (en) * 2021-04-08 2022-10-13 Nicholas C. Dracopoli Method of detecting cancer using genome-wide cfdna fragmentation profiles
JP2024523401A (ja) 2021-06-21 2024-06-28 ガーダント ヘルス， インコーポレイテッド コピー数情報に基づく組織起源分析のための方法および組成物
WO2023056065A1 (en) 2021-09-30 2023-04-06 Guardant Health, Inc. Compositions and methods for synthesis and use of probes targeting nucleic acid rearrangements
WO2023197004A1 (en) 2022-04-07 2023-10-12 Guardant Health, Inc. Detecting the presence of a tumor based on methylation status of cell-free nucleic acid molecules
GB202205710D0 (en) 2022-04-19 2022-06-01 Univ Of Essex Enterprises Limited Cell-free DNA-based methods
JP2025522763A (ja) 2022-06-30 2025-07-17 ガーダント ヘルス， インコーポレイテッド 異常にメチル化されたｄｎａの富化
WO2024056720A1 (en) 2022-09-13 2024-03-21 Medizinische Universität Graz Determining of health status and treatment monitoring with cell-free dna
EP4594522A2 (en) 2022-09-27 2025-08-06 Guardant Health, Inc. Methods for quantifying immune cell dna
JP2025542261A (ja) 2022-12-22 2025-12-25 ガーダント ヘルス， インコーポレイテッド 統合された対象および全ゲノム体細胞およびｄｎａメチル化シーケンシングワークフロー
WO2024137880A2 (en) 2022-12-22 2024-06-27 Guardant Health, Inc. Methods involving methylation preserving amplification with error correction
WO2024233502A1 (en) 2023-05-05 2024-11-14 Guardant Health, Inc. Cell-free dna blood-based test for cancer screening
WO2025029475A1 (en) 2023-07-28 2025-02-06 Guardant Health, Inc. Methods to enrich nucleotide variants by negative selection
WO2025038399A1 (en) 2023-08-11 2025-02-20 Guardant Health, Inc. Methylated enrichment methods for single-molecule genetic and epigenetic sequencing
CN117230165A (zh) * 2023-09-01 2023-12-15 深圳湾实验室 一种无创产前检测胎儿染色体拷贝数异常的优化方法
WO2025064706A1 (en) 2023-09-19 2025-03-27 Guardant Health, Inc. Detecting the presence of a tumor based on methylation status of cell-free nucleic acid molecules
WO2025076452A1 (en) 2023-10-06 2025-04-10 Guardant Health, Inc. Detecting tumor-related information based on methylation status of cell-free nucleic acid molecules
WO2025137389A2 (en) 2023-12-22 2025-06-26 Guardant Health, Inc. Methods for targeted single-molecule genetic and epigenetic sequencing
WO2025207817A1 (en) 2024-03-26 2025-10-02 Guardant Health, Inc. Method of determining the likelihood of a disease by combining biomarkers and imaging
WO2025208044A1 (en) 2024-03-28 2025-10-02 Guardant Health, Inc. Methods for cancer detection using molecular patterns
WO2025235889A1 (en) 2024-05-10 2025-11-13 Guardant Health, Inc. Methods involving multiplexed pooled pcr
WO2025250544A1 (en) 2024-05-31 2025-12-04 Guardant Health, Inc. Methods for analyzing chromatin architecture in tissue to boost detection of cancer associated signals in cell-free dna

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2013177086A1 (en) * 2012-05-21 2013-11-28 Sequenom, Inc. Methods and processes for non-invasive assessment of genetic variations

Family Cites Families (55)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB0016742D0 (en) * 2000-07-10 2000-08-30 Simeg Limited Diagnostic method
JP2002272497A (ja) 2001-03-15 2002-09-24 Venture Link Co Ltd 癌の診断方法、およびその診断用ベクター
US6927028B2 (en) 2001-08-31 2005-08-09 Chinese University Of Hong Kong Non-invasive methods for detecting non-host DNA in a host using epigenetic differences between the host and non-host DNA
EP1599608A4 (en) 2003-03-05 2007-07-18 Genetic Technologies Ltd Identification of fetal DNA and fetal cell marker in maternal plasma or serum
ATE435301T1 (de) 2003-10-16 2009-07-15 Sequenom Inc Nicht invasiver nachweis fötaler genetischer merkmale
US20070122823A1 (en) 2005-09-01 2007-05-31 Bianchi Diana W Amniotic fluid cell-free fetal DNA fragment size pattern for prenatal diagnosis
ES2739484T3 (es) 2006-02-02 2020-01-31 Univ Leland Stanford Junior Prueba genética fetal no invasiva mediante análisis digital
CA2647793C (en) 2006-02-28 2016-07-05 University Of Louisville Research Foundation Detecting fetal chromosomal abnormalities using tandem single nucleotide polymorphisms
TWI335354B (en) 2006-09-27 2011-01-01 Univ Hong Kong Chinese Methods for the detection of the degree of the methylation of a target dna and kits
US7842482B2 (en) 2007-02-26 2010-11-30 The Chinese University Of Hong Kong Methods and kits for diagnosis, prognosis or monitoring of Epstein-Barr virus (EBV)-associated cancer
EP3892736A1 (en) 2007-07-23 2021-10-13 The Chinese University of Hong Kong Determining a nucleic acid sequence imbalance
US12180549B2 (en) 2007-07-23 2024-12-31 The Chinese University Of Hong Kong Diagnosing fetal chromosomal aneuploidy using genomic sequencing
US20090053719A1 (en) 2007-08-03 2009-02-26 The Chinese University Of Hong Kong Analysis of nucleic acids by digital pcr
US8748100B2 (en) 2007-08-30 2014-06-10 The Chinese University Of Hong Kong Methods and kits for selectively amplifying, detecting or quantifying target DNA with specific end sequences
WO2009051842A2 (en) 2007-10-18 2009-04-23 The Johns Hopkins University Detection of cancer by measuring genomic copy number and strand length in cell-free dna
US20100041048A1 (en) 2008-07-31 2010-02-18 The Johns Hopkins University Circulating Mutant DNA to Assess Tumor Dynamics
US8476013B2 (en) * 2008-09-16 2013-07-02 Sequenom, Inc. Processes and compositions for methylation-based acid enrichment of fetal nucleic acid from a maternal sample useful for non-invasive prenatal diagnoses
EP2952589B1 (en) 2008-09-20 2018-02-14 The Board of Trustees of The Leland Stanford Junior University Noninvasive diagnosis of fetal aneuploidy by sequencing
US8835110B2 (en) 2008-11-04 2014-09-16 The Johns Hopkins University DNA integrity assay (DIA) for cancer diagnostics, using confocal fluorescence spectroscopy
WO2010112316A1 (en) 2009-03-31 2010-10-07 Oridis Biomed Forschungs- Und Entwicklungs Gmbh Method for diagnosis of cancer and monitoring of cancer treatments
WO2011053790A2 (en) 2009-10-30 2011-05-05 Fluidigm Corporation Assay of closely linked targets in fetal diagnosis and coincidence detection assay for genetic analysis
CN102770558B (zh) 2009-11-05 2016-04-06 香港中文大学 由母本生物样品进行胎儿基因组的分析
CA2780016C (en) 2009-11-06 2017-09-19 The Chinese University Of Hong Kong Size-based genomic analysis
WO2011090556A1 (en) 2010-01-19 2011-07-28 Verinata Health, Inc. Methods for determining fraction of fetal nucleic acid in maternal samples
US9260745B2 (en) 2010-01-19 2016-02-16 Verinata Health, Inc. Detecting and classifying copy number variation
EP2536854B1 (en) * 2010-02-18 2017-07-19 The Johns Hopkins University Personalized tumor biomarkers
WO2012027483A2 (en) * 2010-08-24 2012-03-01 Bio Dx, Inc. Defining diagnostic and therapeutic targets of conserved free floating fetal dna in maternal circulating blood
EP2426217A1 (en) 2010-09-03 2012-03-07 Centre National de la Recherche Scientifique (CNRS) Analytical methods for cell free nucleic acids and applications
KR102040307B1 (ko) 2010-11-30 2019-11-27 더 차이니즈 유니버시티 오브 홍콩 암과 연관된 유전적 또는 분자적 이상들의 검출
WO2013043922A1 (en) 2011-09-22 2013-03-28 ImmuMetrix, LLC Compositions and methods for analyzing heterogeneous samples
US10196681B2 (en) 2011-10-06 2019-02-05 Sequenom, Inc. Methods and processes for non-invasive assessment of genetic variations
WO2013060762A1 (en) 2011-10-25 2013-05-02 Roche Diagnostics Gmbh Method for diagnosing a disease based on plasma-dna distribution
US9757458B2 (en) 2011-12-05 2017-09-12 Immunomedics, Inc. Crosslinking of CD22 by epratuzumab triggers BCR signaling and caspase-dependent apoptosis in hematopoietic cancer cells
US20130252835A1 (en) 2012-01-27 2013-09-26 Lian Chye Winston Koh Methods for profiling and quantitating cell-free rna
US9605313B2 (en) * 2012-03-02 2017-03-28 Sequenom, Inc. Methods and processes for non-invasive assessment of genetic variations
US9892230B2 (en) 2012-03-08 2018-02-13 The Chinese University Of Hong Kong Size-based analysis of fetal or tumor DNA fraction in plasma
CA2866324C (en) 2012-03-13 2019-01-15 The Chinese University Of Hong Kong Methods for analyzing massively parallel sequencing data for noninvasive prenatal diagnosis
US20150105267A1 (en) * 2012-05-24 2015-04-16 University Of Washington Through Its Center For Commercialization Whole genome sequencing of a human fetus
US11261494B2 (en) * 2012-06-21 2022-03-01 The Chinese University Of Hong Kong Method of measuring a fractional concentration of tumor DNA
KR102393608B1 (ko) 2012-09-04 2022-05-03 가던트 헬쓰, 인크. 희귀 돌연변이 및 카피수 변이를 검출하기 위한 시스템 및 방법
US20160004814A1 (en) * 2012-09-05 2016-01-07 University Of Washington Through Its Center For Commercialization Methods and compositions related to regulation of nucleic acids
US9732390B2 (en) 2012-09-20 2017-08-15 The Chinese University Of Hong Kong Non-invasive determination of methylome of fetus or tumor from plasma
ES2665273T5 (es) 2012-09-20 2023-10-02 Univ Hong Kong Chinese Determinación no invasiva de metiloma del feto o tumor de plasma
AU2014268377B2 (en) * 2013-05-24 2020-10-08 Sequenom, Inc. Methods and processes for non-invasive assessment of genetic variations
ES2704682T5 (es) * 2013-05-29 2023-11-10 Chronix Biomedical Detección y cuantificación de ADN extracelular del donante en la circulación de receptores de trasplantes de órganos
US10262755B2 (en) 2014-04-21 2019-04-16 Natera, Inc. Detecting cancer mutations and aneuploidy in chromosomal segments
CA2945962C (en) * 2014-04-21 2023-08-29 Natera, Inc. Detecting mutations and ploidy in chromosomal segments
IL249095B2 (en) * 2014-05-30 2023-10-01 Verinata Health Inc Detection of subchromosomal aneuploidy in the fetus and variations in the number of copies
TWI813141B (zh) 2014-07-18 2023-08-21 香港中文大學 Ｄｎａ混合物中之組織甲基化模式分析
CN107002122B (zh) 2014-07-25 2023-09-19 华盛顿大学 确定导致无细胞dna的产生的组织和/或细胞类型的方法以及使用其鉴定疾病或紊乱的方法
US11242559B2 (en) 2015-01-13 2022-02-08 The Chinese University Of Hong Kong Method of nuclear DNA and mitochondrial DNA analysis
US10364467B2 (en) 2015-01-13 2019-07-30 The Chinese University Of Hong Kong Using size and number aberrations in plasma DNA for detecting cancer
US10319463B2 (en) 2015-01-23 2019-06-11 The Chinese University Of Hong Kong Combined size- and count-based analysis of maternal plasma for detection of fetal subchromosomal aberrations
CA2976303A1 (en) 2015-02-10 2016-08-18 The Chinese University Of Hong Kong Detecting mutations for cancer screening and fetal analysis
EP3325664B1 (en) 2015-07-23 2021-12-29 The Chinese University Of Hong Kong Analysis of fragmentation patterns of cell-free dna

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2013177086A1 (en) * 2012-05-21 2013-11-28 Sequenom, Inc. Methods and processes for non-invasive assessment of genetic variations

Non-Patent Citations (3)

* Cited by examiner, † Cited by third party
Title
CLAUDIA H. D. METZ ET AL, "Ultradeep sequencing detects GNAQ and GNA11 mutations in cell-free DNA from plasma of patients with uveal melanoma", CANCER MEDICINE, GB, (2013-02-14), vol. 2, no. 2, pages 208 - 215 *
R. J. LEARY ET AL, "Development of Personalized Tumor Biomarkers Using Massively Parallel Sequencing", SCIENCE TRANSLATIONAL MEDICINE, US, (2010-02-24), vol. 2, no. 20, 4, pages 20ra14 - 20ra14 *
T. FORSHEW ET AL, "Noninvasive Identification and Monitoring of Cancer Mutations by Targeted Deep Sequencing of Plasma DNA", SCIENCE TRANSLATIONAL MEDICINE, US, (2012-05-30), vol. 4, no. 136, pages 136ra68 - 136ra68 *

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