AU2014273091B2 - A LAGLIDADG homing endonuclease cleaving the T cell receptor alpha gene and uses thereof - Google Patents

A LAGLIDADG homing endonuclease cleaving the T cell receptor alpha gene and uses thereof Download PDF

Info

Publication number
AU2014273091B2
AU2014273091B2 AU2014273091A AU2014273091A AU2014273091B2 AU 2014273091 B2 AU2014273091 B2 AU 2014273091B2 AU 2014273091 A AU2014273091 A AU 2014273091A AU 2014273091 A AU2014273091 A AU 2014273091A AU 2014273091 B2 AU2014273091 B2 AU 2014273091B2
Authority
AU
Australia
Prior art keywords
cell
cells
dna
sequence
seq
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Active
Application number
AU2014273091A
Other languages
English (en)
Other versions
AU2014273091A1 (en
Inventor
Alexander ASTRAKHAN
Jordan JARJOUR
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Cellectis SA
Precision Genome Engineering Inc
Original Assignee
Cellectis SA
Precision Genome Engineering Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Cellectis SA, Precision Genome Engineering Inc filed Critical Cellectis SA
Publication of AU2014273091A1 publication Critical patent/AU2014273091A1/en
Assigned to PRECISION GENOME ENGINEERING, INC., CELLECTIS reassignment PRECISION GENOME ENGINEERING, INC. Amend patent request/document other than specification (104) Assignors: CELLECTIS, PREGENEN INC.
Application granted granted Critical
Publication of AU2014273091B2 publication Critical patent/AU2014273091B2/en
Active legal-status Critical Current
Anticipated expiration legal-status Critical

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
    • C12N9/22—Ribonucleases [RNase]; Deoxyribonucleases [DNase]
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K38/00—Medicinal preparations containing peptides
    • A61K38/16—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • A61K38/43—Enzymes; Proenzymes; Derivatives thereof
    • A61K38/46—Hydrolases (3)
    • A61K38/465—Hydrolases (3) acting on ester bonds (3.1), e.g. lipases, ribonucleases
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P35/00—Antineoplastic agents
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K48/00—Medicinal preparations containing genetic material which is inserted into cells of the living body to treat genetic diseases; Gene therapy

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Engineering & Computer Science (AREA)
  • Medicinal Chemistry (AREA)
  • Organic Chemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Molecular Biology (AREA)
  • Zoology (AREA)
  • Genetics & Genomics (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Public Health (AREA)
  • Veterinary Medicine (AREA)
  • Animal Behavior & Ethology (AREA)
  • General Engineering & Computer Science (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Epidemiology (AREA)
  • Immunology (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
  • Peptides Or Proteins (AREA)
AU2014273091A 2013-05-31 2014-05-28 A LAGLIDADG homing endonuclease cleaving the T cell receptor alpha gene and uses thereof Active AU2014273091B2 (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
DKPA201370303 2013-05-31
DKPA201370303 2013-05-31
PCT/EP2014/061189 WO2014191527A1 (en) 2013-05-31 2014-05-28 A laglidadg homing endonuclease cleaving the t cell receptor alpha gene and uses thereof

Publications (2)

Publication Number Publication Date
AU2014273091A1 AU2014273091A1 (en) 2015-12-03
AU2014273091B2 true AU2014273091B2 (en) 2019-12-12

Family

ID=48628226

Family Applications (1)

Application Number Title Priority Date Filing Date
AU2014273091A Active AU2014273091B2 (en) 2013-05-31 2014-05-28 A LAGLIDADG homing endonuclease cleaving the T cell receptor alpha gene and uses thereof

Country Status (8)

Country Link
US (1) US10000746B2 (https=)
EP (1) EP3004338B1 (https=)
JP (1) JP6450371B2 (https=)
AU (1) AU2014273091B2 (https=)
CA (1) CA2913872C (https=)
DK (1) DK3004338T3 (https=)
ES (1) ES2716867T3 (https=)
WO (1) WO2014191527A1 (https=)

Families Citing this family (82)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP2694089B1 (en) * 2011-04-05 2024-06-05 Cellectis New tale-protein scaffolds and uses thereof
JP6261500B2 (ja) 2011-07-22 2018-01-17 プレジデント アンド フェローズ オブ ハーバード カレッジ ヌクレアーゼ切断特異性の評価および改善
US10704021B2 (en) 2012-03-15 2020-07-07 Flodesign Sonics, Inc. Acoustic perfusion devices
US20150044192A1 (en) 2013-08-09 2015-02-12 President And Fellows Of Harvard College Methods for identifying a target site of a cas9 nuclease
US9359599B2 (en) 2013-08-22 2016-06-07 President And Fellows Of Harvard College Engineered transcription activator-like effector (TALE) domains and uses thereof
US9526784B2 (en) 2013-09-06 2016-12-27 President And Fellows Of Harvard College Delivery system for functional nucleases
US9340799B2 (en) 2013-09-06 2016-05-17 President And Fellows Of Harvard College MRNA-sensing switchable gRNAs
US9388430B2 (en) 2013-09-06 2016-07-12 President And Fellows Of Harvard College Cas9-recombinase fusion proteins and uses thereof
AU2014346559B2 (en) 2013-11-07 2020-07-09 Editas Medicine,Inc. CRISPR-related methods and compositions with governing gRNAs
US20150165054A1 (en) 2013-12-12 2015-06-18 President And Fellows Of Harvard College Methods for correcting caspase-9 point mutations
WO2015105955A1 (en) 2014-01-08 2015-07-16 Flodesign Sonics, Inc. Acoustophoresis device with dual acoustophoretic chamber
WO2016022363A2 (en) 2014-07-30 2016-02-11 President And Fellows Of Harvard College Cas9 proteins including ligand-dependent inteins
US11708572B2 (en) 2015-04-29 2023-07-25 Flodesign Sonics, Inc. Acoustic cell separation techniques and processes
CN117070468A (zh) 2015-10-05 2023-11-17 精密生物科学公司 包含经修饰的人T细胞受体α恒定区基因的经遗传修饰的细胞
EP3359660B1 (en) 2015-10-05 2019-12-04 Precision Biosciences, Inc. Engineered meganucleases with recognition sequences found in the human t cell receptor alpha constant region gene
JP7109784B2 (ja) 2015-10-23 2022-08-01 プレジデント アンド フェローズ オブ ハーバード カレッジ 遺伝子編集のための進化したＣａｓ９蛋白質
EA201891338A1 (ru) 2015-12-04 2018-12-28 Новартис Аг Композиции и способы для иммуноонкологии
MX2018010924A (es) * 2016-03-11 2019-02-13 Bluebird Bio Inc Células efectoras inmunitarias con edición genómica.
US10188749B2 (en) 2016-04-14 2019-01-29 Fred Hutchinson Cancer Research Center Compositions and methods to program therapeutic cells using targeted nucleic acid nanocarriers
AU2017250295B2 (en) * 2016-04-14 2022-08-25 Fred Hutchinson Cancer Center Compositions and methods to program therapeutic cells using targeted nucleic acid nanocarriers
US11214789B2 (en) 2016-05-03 2022-01-04 Flodesign Sonics, Inc. Concentration and washing of particles with acoustics
JP2019525759A (ja) * 2016-07-25 2019-09-12 ブルーバード バイオ， インコーポレイテッド Ｂｃｌ１１ａホーミングエンドヌクレアーゼバリアント、組成物、および使用方法
KR102547316B1 (ko) 2016-08-03 2023-06-23 프레지던트 앤드 펠로우즈 오브 하바드 칼리지 아데노신 핵염기 편집제 및 그의 용도
US11661590B2 (en) 2016-08-09 2023-05-30 President And Fellows Of Harvard College Programmable CAS9-recombinase fusion proteins and uses thereof
WO2018035141A1 (en) 2016-08-16 2018-02-22 Bluebird Bio, Inc. Il-10 receptor alpha homing endonuclease variants, compositions, and methods of use
WO2018039333A1 (en) * 2016-08-23 2018-03-01 Bluebird Bio, Inc. Tim3 homing endonuclease variants, compositions, and methods of use
US11542509B2 (en) 2016-08-24 2023-01-03 President And Fellows Of Harvard College Incorporation of unnatural amino acids into proteins using base editing
KR102622910B1 (ko) 2016-09-08 2024-01-10 2세븐티 바이오, 인코포레이티드 Pd-1 호밍 엔도뉴클레아제 변이체, 조성물 및 사용 방법
WO2018071565A1 (en) * 2016-10-11 2018-04-19 Bluebird Bio, Inc. TCRa HOMING ENDONUCLEASE VARIANTS
EP3526320A1 (en) 2016-10-14 2019-08-21 President and Fellows of Harvard College Aav delivery of nucleobase editors
US11530395B2 (en) * 2016-10-17 2022-12-20 2Seventy Bio, Inc. TGFBetaR2 endonuclease variants, compositions, and methods of use
WO2018073393A2 (en) 2016-10-19 2018-04-26 Cellectis Tal-effector nuclease (talen) -modified allogenic cells suitable for therapy
CN110352068A (zh) 2016-12-02 2019-10-18 南加利福尼亚大学 合成的免疫受体及其使用方法
WO2018119359A1 (en) 2016-12-23 2018-06-28 President And Fellows Of Harvard College Editing of ccr5 receptor gene to protect against hiv infection
JP7348063B2 (ja) 2017-01-05 2023-09-20 フレッド ハッチンソン キャンサー センター ワクチン効力を改善するためのシステム及び方法
JP2020510038A (ja) 2017-03-09 2020-04-02 プレジデント アンド フェローズ オブ ハーバード カレッジ がんワクチン
EP3592853A1 (en) 2017-03-09 2020-01-15 President and Fellows of Harvard College Suppression of pain by gene editing
JP2020510439A (ja) 2017-03-10 2020-04-09 プレジデント アンド フェローズ オブ ハーバード カレッジ シトシンからグアニンへの塩基編集因子
WO2018175636A2 (en) 2017-03-22 2018-09-27 Novartis Ag Compositions and methods for immunooncology
CA3057192A1 (en) 2017-03-23 2018-09-27 President And Fellows Of Harvard College Nucleobase editors comprising nucleic acid programmable dna binding proteins
US11767512B2 (en) 2017-04-13 2023-09-26 Cellectis Sequence specific reagents targeting CCR5 in primary hematopoietic cells
WO2018209320A1 (en) 2017-05-12 2018-11-15 President And Fellows Of Harvard College Aptazyme-embedded guide rnas for use with crispr-cas9 in genome editing and transcriptional activation
IL313037A (en) 2017-05-25 2024-07-01 2Seventy Bio Inc Cblb endonuclease variants, compositions, and methods of use
CA3068465A1 (en) 2017-06-30 2019-01-03 Precision Biosciences, Inc. Genetically-modified t cells comprising a modified intron in the t cell receptor alpha gene
WO2019023680A1 (en) 2017-07-28 2019-01-31 President And Fellows Of Harvard College METHODS AND COMPOSITIONS FOR EVOLUTION OF BASIC EDITORS USING PHAGE-ASSISTED CONTINUOUS EVOLUTION (PACE)
EP3676376B1 (en) 2017-08-30 2025-01-15 President and Fellows of Harvard College High efficiency base editors comprising gam
WO2019070974A1 (en) * 2017-10-04 2019-04-11 Bluebird Bio, Inc. PCSK9 ENDONUCLEASE VARIANTS, COMPOSITIONS AND METHODS OF USE
WO2019073965A1 (ja) 2017-10-10 2019-04-18 国立大学法人広島大学 エフェクターＴ細胞（Ｔｅｆｆ）抗原受容体を用いた抗原特異的制御性Ｔ細胞（Ｔｒｅｇ）の作出技術
TWI814746B (zh) * 2017-10-10 2023-09-11 國立大學法人廣島大學 使用鉑talen之t細胞受體之完全置換技術
KR20250107288A (ko) 2017-10-16 2025-07-11 더 브로드 인스티튜트, 인코퍼레이티드 아데노신 염기 편집제의 용도
WO2019118921A1 (en) 2017-12-14 2019-06-20 Flodesign Sonics, Inc. Acoustic transducer drive and controller
WO2019118949A1 (en) 2017-12-15 2019-06-20 The Broad Institute, Inc. Systems and methods for predicting repair outcomes in genetic engineering
US20210002621A1 (en) * 2017-12-20 2021-01-07 Bluebird Bio, Inc. Ctla4 homing endonuclease variants, compositions, and methods of use
CN111629715A (zh) 2018-01-18 2020-09-04 弗莱德哈钦森癌症研究中心 通过调节细胞活化状态改变体内免疫细胞的炎症状态
CA3095795A1 (en) 2018-04-12 2019-10-17 Precision Biosciences, Inc. Optimized engineered nucleases having specificity for the human t cell receptor alpha constant region gene
EP3797160A1 (en) 2018-05-23 2021-03-31 The Broad Institute Inc. Base editors and uses thereof
JP2021527409A (ja) 2018-06-14 2021-10-14 ブルーバード バイオ， インコーポレイテッド Ｃｄ７９ａキメラ抗原受容体
US12522807B2 (en) 2018-07-09 2026-01-13 The Broad Institute, Inc. RNA programmable epigenetic RNA modifiers and uses thereof
WO2020072059A1 (en) * 2018-10-04 2020-04-09 Bluebird Bio, Inc. Cblb endonuclease variants, compositions, and methods of use
US12331320B2 (en) 2018-10-10 2025-06-17 The Research Foundation For The State University Of New York Genome edited cancer cell vaccines
WO2020092453A1 (en) 2018-10-29 2020-05-07 The Broad Institute, Inc. Nucleobase editors comprising geocas9 and uses thereof
US12577547B2 (en) 2018-12-10 2026-03-17 Regeneron Pharmaceuticals, Inc. PDCD-1 homing endonuclease variants
WO2020123371A2 (en) 2018-12-10 2020-06-18 Bluebird Bio, Inc. Homing endonuclease variants
US12351837B2 (en) 2019-01-23 2025-07-08 The Broad Institute, Inc. Supernegatively charged proteins and uses thereof
JP7657726B2 (ja) 2019-03-19 2025-04-07 ザ ブロード インスティテュート，インコーポレーテッド 編集ヌクレオチド配列を編集するための方法および組成物
JP7286796B2 (ja) 2019-04-03 2023-06-05 プレシジョン バイオサイエンシズ，インク． マイクロｒｎａ適合ｓｈｒｎａ（ｓｈｒｎａｍｉｒ）を含む遺伝子改変免疫細胞
US12473543B2 (en) 2019-04-17 2025-11-18 The Broad Institute, Inc. Adenine base editors with reduced off-target effects
EP4004216A1 (en) 2019-07-25 2022-06-01 Precision BioSciences, Inc. Compositions and methods for sequential stacking of nucleic acid sequences into a genomic locus
AU2020333851A1 (en) 2019-08-20 2022-03-31 Precision Biosciences, Inc. Lymphodepletion dosing regimens for cellular immunotherapies
US12435330B2 (en) 2019-10-10 2025-10-07 The Broad Institute, Inc. Methods and compositions for prime editing RNA
WO2021087305A1 (en) 2019-10-30 2021-05-06 Precision Biosciences, Inc. Cd20 chimeric antigen receptors and methods of use for immunotherapy
DE112021002672T5 (de) 2020-05-08 2023-04-13 President And Fellows Of Harvard College Vefahren und zusammensetzungen zum gleichzeitigen editieren beider stränge einer doppelsträngigen nukleotid-zielsequenz
WO2022035793A1 (en) 2020-08-10 2022-02-17 Precision Biosciences, Inc. Antibodies and fragments specific for b-cell maturation antigen and uses thereof
US20240336904A1 (en) * 2020-12-21 2024-10-10 2Seventy Bio, Inc. Compositions and methods for site-directed mutagenesis
WO2022165111A1 (en) 2021-01-28 2022-08-04 Precision Biosciences, Inc. Modulation of tgf beta signaling in genetically-modified eukaryotic cells
EP4426832A1 (en) 2021-11-03 2024-09-11 The J. David Gladstone Institutes, A Testamentary Trust Established under The Will of J. David Gladstone Precise genome editing using retrons
WO2023081767A1 (en) 2021-11-05 2023-05-11 Precision Biosciences, Inc. Methods for immunotherapy
WO2023094435A1 (en) 2021-11-23 2023-06-01 Cellectis Sa New tale protein scaffolds with improved on-target/off-target activity ratios
WO2023141602A2 (en) 2022-01-21 2023-07-27 Renagade Therapeutics Management Inc. Engineered retrons and methods of use
WO2024044723A1 (en) 2022-08-25 2024-02-29 Renagade Therapeutics Management Inc. Engineered retrons and methods of use
WO2024148167A1 (en) 2023-01-05 2024-07-11 Precision Biosciences, Inc. Optimized engineered meganucleases having specificity for the human t cell receptor alpha constant region gene
WO2026046724A1 (en) 2024-08-30 2026-03-05 Cellectis Sa Tale protein scaffolds involving fusions of monopartite and bipartite nls

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2011156430A2 (en) * 2010-06-07 2011-12-15 Fred Hutchinson Cancer Research Center Generation and expression of engineered i-onui endonuclease and its homologues and uses thereof

Family Cites Families (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US8956828B2 (en) * 2009-11-10 2015-02-17 Sangamo Biosciences, Inc. Targeted disruption of T cell receptor genes using engineered zinc finger protein nucleases
EP2694089B1 (en) * 2011-04-05 2024-06-05 Cellectis New tale-protein scaffolds and uses thereof
US9540623B2 (en) * 2011-07-08 2017-01-10 Cellectis Method for increasing the efficiency of double-strand-break induced mutagenesis
EP2776560A2 (en) * 2011-11-07 2014-09-17 The University of Western Ontario Endonuclease for genome editing

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2011156430A2 (en) * 2010-06-07 2011-12-15 Fred Hutchinson Cancer Research Center Generation and expression of engineered i-onui endonuclease and its homologues and uses thereof

Non-Patent Citations (6)

* Cited by examiner, † Cited by third party
Title
BAXTER SARAH ET AL, "Engineering domain fusion chimeras from I-OnuI family LAGLIDADG homing endonucleases.", NUCLEIC ACIDS RESEARCH SEP 2012, (2012-09), vol. 40, no. 16, ISSN 1362-4962, pages 7985 - 8000 *
HAFEZ MOHAMED ET AL, "Homing endonucleases: DNA scissors on a mission", GENOME, (2012-08), vol. 55, no. 8, pages 553 - 569 *
PROVASI ELENA ET AL, "TCR Gene Editing Results in Effective Immunotherapy of Leukemia without the Development of GvHD", BLOOD, AMERICAN SOCIETY OF HEMATOLOGY, US, (2011-12-13), vol. 118, no. 21, ISSN 0006-4971, page 307 *
RYO TAKEUCHI ET AL, "Tapping natural reservoirs of homing endonucleases for targeted gene modification", PROC. NAT. ACAD. SCI. 2011, vol. 108, no. 32, pages 13077 - 13082 *
S. BOISSEL ET AL, "megaTALs: a rare-cleaving nuclease architecture for therapeutic genome engineering", NUCLEIC ACIDS RESEARCH, (2013-11-26), vol. 42, no. 4, doi:10.1093/nar/gkt1224, ISSN 0305-1048, pages 2591 - 2601 *
TORIKAI H ET AL, "A foundation for universal T-cell based immunotherapy: T cells engineered to express a CD19-specific chimeric-antigen-receptor and eliminate expression of endogenous TCR.", BLOOD. 2012, vol. 119, no. 24, pages 5697 - 5705 *

Also Published As

Publication number Publication date
DK3004338T3 (en) 2019-04-08
AU2014273091A1 (en) 2015-12-03
HK1223394A1 (en) 2017-07-28
CA2913872A1 (en) 2014-12-04
WO2014191527A1 (en) 2014-12-04
EP3004338B1 (en) 2019-01-02
ES2716867T3 (es) 2019-06-17
JP2016520320A (ja) 2016-07-14
US10000746B2 (en) 2018-06-19
US20160130569A1 (en) 2016-05-12
JP6450371B2 (ja) 2019-01-09
CA2913872C (en) 2022-01-18
EP3004338A1 (en) 2016-04-13

Similar Documents

Publication Publication Date Title
AU2014273091B2 (en) A LAGLIDADG homing endonuclease cleaving the T cell receptor alpha gene and uses thereof
EP3004149B1 (en) A laglidadg homing endonuclease cleaving the c-c chemokine receptor type-5 (ccr5) gene and uses thereof
CN119490976B (zh) 改造的cascade组分和cascade复合体
CN102264915B (zh) 失活谷氨酰胺合成酶基因表达的方法和组合物
WO2020041249A1 (en) Engineered target specific base editors
JP2019520844A (ja) ゲノムｄｎａを改変するための方法および組成物
JP7641233B2 (ja) 改善された遺伝子編集のための組成物及び方法
US20130189759A1 (en) Meganucleases variants cleaving a dna target sequence in the nanog gene and uses thereof
JP2024532784A (ja) 新規なｏｍｎｉ－１１５、１２４、１２７、１４４～１４９、１５９、２１８、２３７、２４８、２５１～２５３及び２５９ｃｒｉｓｐｒヌクレアーゼ
HK1223394B (en) A laglidadg homing endonuclease cleaving the t cell receptor alpha gene and uses thereof
Jang et al. Genome editing systems in novel therapies
HK1223373B (en) A laglidadg homing endonuclease cleaving the c-c chemokine receptor type-5 (ccr5) gene and uses thereof
US20160138047A1 (en) Improved polynucleotide sequences encoding tale repeats
WO2012007848A2 (en) Meganuclease variants cleaving a dna target sequence in the was gene and uses thereof
HK40126396A (en) Compositions and methods for improved gene editing
Class et al. Patent application title: A LAGLIDADG HOMING ENDONUCLEASE CLEAVING THE T CELL RECEPTOR ALPHA GENE AND USES THEREOF Inventors: Jordan Jarjour (Seattle, WA, US) Alexander Astrakhan (Seattle, WA, US)
Checketts Nuclease-Mediated Targeted Gene Insertion at the Adenosine Deaminase Locus in Primary Cells

Legal Events

Date Code Title Description
FGA Letters patent sealed or granted (standard patent)