AU2013346504A1 - Method for targeted modification of algae genomes - Google Patents

Method for targeted modification of algae genomes Download PDF

Info

Publication number
AU2013346504A1
AU2013346504A1 AU2013346504A AU2013346504A AU2013346504A1 AU 2013346504 A1 AU2013346504 A1 AU 2013346504A1 AU 2013346504 A AU2013346504 A AU 2013346504A AU 2013346504 A AU2013346504 A AU 2013346504A AU 2013346504 A1 AU2013346504 A1 AU 2013346504A1
Authority
AU
Australia
Prior art keywords
tale
gene
seq
nuclease
sequence
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Abandoned
Application number
AU2013346504A
Other languages
English (en)
Inventor
Fayza Daboussi
Philippe Duchateau
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Cellectis SA
TotalEnergies Marketing Services SA
Original Assignee
Cellectis SA
Total Marketing Services SA
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Cellectis SA, Total Marketing Services SA filed Critical Cellectis SA
Publication of AU2013346504A1 publication Critical patent/AU2013346504A1/en
Assigned to CELLECTIS, TOTAL MARKETING SERVICES reassignment CELLECTIS Amend patent request/document other than specification (104) Assignors: TOTAL MARKETING SERVICES
Abandoned legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8201—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation
    • C12N15/8213—Targeted insertion of genes into the plant genome by homologous recombination
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/195—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/12—Unicellular algae; Culture media therefor
    • C12N1/125—Unicellular algae isolates
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/52—Genes encoding for enzymes or proenzymes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/12—Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
    • C12N9/1241—Nucleotidyltransferases (2.7.7)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
    • C12N9/22—Ribonucleases [RNase]; Deoxyribonucleases [DNase]
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/80—Fusion polypeptide containing a DNA binding domain, e.g. Lacl or Tet-repressor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12R—INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
    • C12R2001/00—Microorganisms ; Processes using microorganisms
    • C12R2001/89—Algae ; Processes using algae
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y207/00—Transferases transferring phosphorus-containing groups (2.7)
    • C12Y207/07—Nucleotidyltransferases (2.7.7)
    • C12Y207/07009—UTP-glucose-1-phosphate uridylyltransferase (2.7.7.9), i.e. UDP-glucose-pyrophosphorylase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y301/00—Hydrolases acting on ester bonds (3.1)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y301/00—Hydrolases acting on ester bonds (3.1)
    • C12Y301/11—Exodeoxyribonucleases producing 5'-phosphomonoesters (3.1.11)
    • C12Y301/11002—Exodeoxyribonuclease III (3.1.11.2)

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Medicinal Chemistry (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Plant Pathology (AREA)
  • Cell Biology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Virology (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Botany (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Enzymes And Modification Thereof (AREA)
AU2013346504A 2012-11-16 2013-11-18 Method for targeted modification of algae genomes Abandoned AU2013346504A1 (en)

Applications Claiming Priority (5)

Application Number Priority Date Filing Date Title
US201261727444P 2012-11-16 2012-11-16
US61/727,444 2012-11-16
US201361839165P 2013-06-25 2013-06-25
US61/839,165 2013-06-25
PCT/IB2013/002974 WO2014076571A2 (en) 2012-11-16 2013-11-18 Method for targeted modification of algae genomes

Publications (1)

Publication Number Publication Date
AU2013346504A1 true AU2013346504A1 (en) 2015-06-18

Family

ID=50231458

Family Applications (1)

Application Number Title Priority Date Filing Date
AU2013346504A Abandoned AU2013346504A1 (en) 2012-11-16 2013-11-18 Method for targeted modification of algae genomes

Country Status (7)

Country Link
US (1) US20160272980A1 (https=)
EP (1) EP2920310A2 (https=)
CN (1) CN105121649A (https=)
AU (1) AU2013346504A1 (https=)
BR (1) BR112015011190A2 (https=)
IN (1) IN2015DN04148A (https=)
WO (1) WO2014076571A2 (https=)

Families Citing this family (11)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CA2915779A1 (en) * 2013-06-25 2014-12-31 Cellectis Modified diatoms for biofuel production
WO2016168756A1 (en) 2015-04-15 2016-10-20 Synthetic Genomics, Inc. Algal chloroplastic srp54 mutants
CN105567681B (zh) * 2015-12-31 2018-08-31 广州赛哲生物科技股份有限公司 一种基于高通量基因测序无创活检病毒的方法及标签接头
WO2018219619A1 (en) * 2017-05-09 2018-12-06 Cyano Biotech Gmbh Method for modifying microcystins and nodularins
JP2021505158A (ja) 2017-12-08 2021-02-18 シンセティック ジェノミクス インコーポレーテッド Ｔｐｒドメイン含有タンパク質の遺伝子改変を介した藻類脂質生産性の改善方法
JP7295864B2 (ja) 2017-12-29 2023-06-21 シンセティック ジェノミクス インコーポレーテッド 成長を改善するための光合成生物の遺伝子調節方法
US11352618B2 (en) 2018-03-23 2022-06-07 Sean Raspet Modified organisms for improved flavor and aroma
WO2019200318A1 (en) * 2018-04-13 2019-10-17 Sean Raspet Modified organisms for improved flavor and aroma
CN112813091A (zh) * 2021-02-03 2021-05-18 山东农业大学 Nat1基因作为筛选标记在卵菌遗传转化中的应用
WO2023198774A1 (en) 2022-04-14 2023-10-19 Institute Of Agricultural Resources And Regional Planning Of The Chinese Academy Of Agricultural Sciences Modified microalgae for enhanced phosphate uptade involving overexpression of psr1 and optionally underexpression of ptc1
EP4638699A1 (en) 2022-12-19 2025-10-29 Hutanbio Ltd. Lipid producing marine microalga

Family Cites Families (16)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4683195A (en) 1986-01-30 1987-07-28 Cetus Corporation Process for amplifying, detecting, and/or-cloning nucleic acid sequences
FR2646438B1 (fr) 1989-03-20 2007-11-02 Pasteur Institut Procede de remplacement specifique d'une copie d'un gene present dans le genome receveur par l'integration d'un gene different de celui ou se fait l'integration
US5516670A (en) 1991-09-30 1996-05-14 Kuehnle; Adelheid R. Magnetophoretic particle delivery method and apparatus for the treatment of cells
WO1997039106A1 (en) * 1996-04-12 1997-10-23 Martek Biosciences Corporation Methods and tools for transformation of eukaryotic algae
US6706394B2 (en) 2000-05-02 2004-03-16 Manfred R. Kuehnle Method and apparatus for manufacture of magnetizable microparticles
US20060078552A1 (en) 2002-03-15 2006-04-13 Sylvain Arnould Hybrid and single chain meganucleases and use thereof
WO2009095742A1 (en) 2008-01-31 2009-08-06 Cellectis New i-crei derived single-chain meganuclease and uses thereof
EP2327771A1 (en) 2005-03-15 2011-06-01 Cellectis I-crel meganuclease variants with modified specificity, method of preparation and uses thereof
WO2008093152A1 (en) 2007-02-01 2008-08-07 Cellectis Obligate heterodimer meganucleases and uses thereof
EP2504439B1 (en) 2009-11-27 2016-03-02 BASF Plant Science Company GmbH Optimized endonucleases and uses thereof
WO2011064751A1 (en) * 2009-11-27 2011-06-03 Basf Plant Science Company Gmbh Chimeric endonucleases and uses thereof
JP6208580B2 (ja) 2010-05-17 2017-10-04 サンガモ セラピューティクス， インコーポレイテッド 新規のｄｎａ結合タンパク質及びその使用
US8608877B2 (en) * 2010-07-27 2013-12-17 General Electric Company Nickel alloy and articles
WO2012017329A2 (en) 2010-08-02 2012-02-09 Cellectis S.A. Method for targeted genomic events in algae
WO2012118717A2 (en) * 2011-02-28 2012-09-07 Seattle Children's Research Institute Coupling endonucleases with end-processing enzymes drive high efficiency gene disruption
EP2694089B1 (en) 2011-04-05 2024-06-05 Cellectis New tale-protein scaffolds and uses thereof

Also Published As

Publication number Publication date
WO2014076571A8 (en) 2014-07-10
IN2015DN04148A (https=) 2015-10-16
EP2920310A2 (en) 2015-09-23
WO2014076571A2 (en) 2014-05-22
US20160272980A1 (en) 2016-09-22
WO2014076571A9 (en) 2015-04-02
BR112015011190A2 (pt) 2017-10-03
WO2014076571A3 (en) 2014-11-06
CN105121649A (zh) 2015-12-02

Similar Documents

Publication Publication Date Title
US20160272980A1 (en) Method for targeted modification of algae genomes
EP3080275B1 (en) Method of selection of transformed diatoms using nuclease
US10087453B2 (en) Modified diatoms for biofuel production
CA2999050C (en) Protected dna templates for gene modification and increased homologous recombination in cells and methods of use
KR20180002852A (ko) 가이드 RNA/Cas 엔도뉴클레아제 시스템
AU2011287293B2 (en) Method for targeted genomic events in algae
US10563232B2 (en) Microorganisms having increased lipid productivity
US20170121742A1 (en) Algal mutants with increased lipid productivity
Dehghani et al. Efficient and stable transformation of Dunaliella pseudosalina by 3 strains of Agrobacterium tumefaciens
JP2021505158A (ja) Ｔｐｒドメイン含有タンパク質の遺伝子改変を介した藻類脂質生産性の改善方法
WO2012158778A1 (en) Doubly secure transgenic algae or cyanobacteria strains to prevent their establishment and spread in natural ecosystems

Legal Events

Date Code Title Description
MK5 Application lapsed section 142(2)(e) - patent request and compl. specification not accepted