BR112015011190A2 - Método de modificação dirigida e célula de alga geneticamente modificada - Google Patents

Método de modificação dirigida e célula de alga geneticamente modificada

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Publication number
BR112015011190A2
BR112015011190A2 BR112015011190A BR112015011190A BR112015011190A2 BR 112015011190 A2 BR112015011190 A2 BR 112015011190A2 BR 112015011190 A BR112015011190 A BR 112015011190A BR 112015011190 A BR112015011190 A BR 112015011190A BR 112015011190 A2 BR112015011190 A2 BR 112015011190A2
Authority
BR
Brazil
Prior art keywords
algae cell
genetically modified
genetic material
directed modification
modification method
Prior art date
Application number
BR112015011190A
Other languages
English (en)
Inventor
Duchateau Philippe
Daboussi Fayza
Original Assignee
Total Marketing Services
Cellectis
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Total Marketing Services, Cellectis filed Critical Total Marketing Services
Publication of BR112015011190A2 publication Critical patent/BR112015011190A2/pt

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8201—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation
    • C12N15/8213—Targeted insertion of genes into the plant genome by homologous recombination
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/195—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/12—Unicellular algae; Culture media therefor
    • C12N1/125—Unicellular algae isolates
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/52—Genes encoding for enzymes or proenzymes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/12—Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
    • C12N9/1241—Nucleotidyltransferases (2.7.7)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
    • C12N9/22—Ribonucleases RNAses, DNAses
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/80—Fusion polypeptide containing a DNA binding domain, e.g. Lacl or Tet-repressor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12R—INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
    • C12R2001/00—Microorganisms ; Processes using microorganisms
    • C12R2001/89—Algae ; Processes using algae
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y207/00—Transferases transferring phosphorus-containing groups (2.7)
    • C12Y207/07—Nucleotidyltransferases (2.7.7)
    • C12Y207/07009—UTP-glucose-1-phosphate uridylyltransferase (2.7.7.9), i.e. UDP-glucose-pyrophosphorylase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y301/00—Hydrolases acting on ester bonds (3.1)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y301/00—Hydrolases acting on ester bonds (3.1)
    • C12Y301/11—Exodeoxyribonucleases producing 5'-phosphomonoesters (3.1.11)
    • C12Y301/11002—Exodeoxyribonuclease III (3.1.11.2)

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • Medicinal Chemistry (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Plant Pathology (AREA)
  • Cell Biology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Botany (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Virology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Enzymes And Modification Thereof (AREA)

Abstract

MÉTODO DE MODIFICAÇÃO DIRIGIDA DO MATERIAL GENÉTICO DE CÉLULAS DE ALGAS E CÉLULA DE ALGA GENETICAMENTE MODIFICADA. A presente invenção refere-se a um método de modificação de material genético em células de algas, que inclui o uso de endonuclease de corte raro para dirigir sequências genômicas específicas. Em particular, a presente invenção refere-se a um método de modificação de material genético em células de algas, em que endonuclease de corte raro, especialmente endonuclease de alojamento ou nuclease TALE, é expressa ao longo de várias gerações para modificar eficientemente as mencionadas sequências de genoma alvo.
BR112015011190A 2012-11-16 2013-11-18 Método de modificação dirigida e célula de alga geneticamente modificada BR112015011190A2 (pt)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US201261727444P 2012-11-16 2012-11-16
US201361839165P 2013-06-25 2013-06-25
PCT/IB2013/002974 WO2014076571A2 (en) 2012-11-16 2013-11-18 Method for targeted modification of algae genomes

Publications (1)

Publication Number Publication Date
BR112015011190A2 true BR112015011190A2 (pt) 2017-10-03

Family

ID=50231458

Family Applications (1)

Application Number Title Priority Date Filing Date
BR112015011190A BR112015011190A2 (pt) 2012-11-16 2013-11-18 Método de modificação dirigida e célula de alga geneticamente modificada

Country Status (7)

Country Link
US (1) US20160272980A1 (pt)
EP (1) EP2920310A2 (pt)
CN (1) CN105121649A (pt)
AU (1) AU2013346504A1 (pt)
BR (1) BR112015011190A2 (pt)
IN (1) IN2015DN04148A (pt)
WO (1) WO2014076571A2 (pt)

Families Citing this family (11)

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AU2014301147B2 (en) * 2013-06-25 2020-07-30 Cellectis Modified diatoms for biofuel production
CA2982848A1 (en) 2015-04-15 2016-10-20 Synthetic Genomics, Inc. Algal chloroplastic srp54 mutants
CN105567681B (zh) * 2015-12-31 2018-08-31 广州赛哲生物科技股份有限公司 一种基于高通量基因测序无创活检病毒的方法及标签接头
AU2018278678B2 (en) * 2017-05-09 2022-02-03 Cyano Biotech Gmbh Method for modifying microcystins and nodularins
BR112020011350A2 (pt) 2017-12-08 2020-11-17 Synthetic Genomics, Inc. aperfeiçoamento da produtividade lipídica de algas através da modificação genética de uma proteína que contém domínio de tpr
EP3732189A4 (en) 2017-12-29 2022-01-19 Synthetic Genomics, Inc. GENETIC MODULATION OF PHOTOSYNTHETIC ORGANISMS FOR ENHANCED GROWTH
US11352618B2 (en) 2018-03-23 2022-06-07 Sean Raspet Modified organisms for improved flavor and aroma
WO2019200318A1 (en) * 2018-04-13 2019-10-17 Sean Raspet Modified organisms for improved flavor and aroma
CN112813091A (zh) * 2021-02-03 2021-05-18 山东农业大学 Nat1基因作为筛选标记在卵菌遗传转化中的应用
WO2023198774A1 (en) 2022-04-14 2023-10-19 Institute Of Agricultural Resources And Regional Planning Of The Chinese Academy Of Agricultural Sciences Modified microalgae for enhanced phosphate uptade involving overexpression of psr1 and optionally underexpression of ptc1
WO2024132882A1 (en) 2022-12-19 2024-06-27 Hutanbio Ltd. Lipid producing marine microalga

Family Cites Families (16)

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US4683195A (en) 1986-01-30 1987-07-28 Cetus Corporation Process for amplifying, detecting, and/or-cloning nucleic acid sequences
FR2646438B1 (fr) 1989-03-20 2007-11-02 Pasteur Institut Procede de remplacement specifique d'une copie d'un gene present dans le genome receveur par l'integration d'un gene different de celui ou se fait l'integration
US5516670A (en) 1991-09-30 1996-05-14 Kuehnle; Adelheid R. Magnetophoretic particle delivery method and apparatus for the treatment of cells
US6027900A (en) * 1996-04-12 2000-02-22 Carnegie Institution Of Washington Methods and tools for transformation of eukaryotic algae
US6706394B2 (en) 2000-05-02 2004-03-16 Manfred R. Kuehnle Method and apparatus for manufacture of magnetizable microparticles
WO2009095742A1 (en) 2008-01-31 2009-08-06 Cellectis New i-crei derived single-chain meganuclease and uses thereof
ATE372379T1 (de) 2002-03-15 2007-09-15 Cellectis Hybride and einzelkettige meganukleasen und deren anwendungen
US7897372B2 (en) 2005-03-15 2011-03-01 Cellectis I-CreI meganuclease variants with modified specificity, method of preparation and uses thereof
WO2008093152A1 (en) 2007-02-01 2008-08-07 Cellectis Obligate heterodimer meganucleases and uses thereof
AU2010325564A1 (en) * 2009-11-27 2012-07-12 Basf Plant Science Company Gmbh Chimeric endonucleases and uses thereof
DE112010004582T5 (de) 2009-11-27 2012-11-29 Basf Plant Science Company Gmbh Optimierte Endonukleasen und Anwendungen davon
CN103025344B (zh) 2010-05-17 2016-06-29 桑格摩生物科学股份有限公司 新型dna-结合蛋白及其用途
US8608877B2 (en) * 2010-07-27 2013-12-17 General Electric Company Nickel alloy and articles
SG187680A1 (en) * 2010-08-02 2013-03-28 Cellectis Method for targeted genomic events in algae
ES2906059T3 (es) * 2011-02-28 2022-04-13 Seattle Childrens Res Institute Endonucleasas de acoplamiento con enzimas de procesamiento de extremos que impulsan la interrupción génica de alta eficiencia
DK2694091T3 (da) 2011-04-05 2019-06-03 Cellectis Fremgangsmåde til fremstilling af kompakte tale-nukleaser og anvendelse heraf

Also Published As

Publication number Publication date
WO2014076571A8 (en) 2014-07-10
CN105121649A (zh) 2015-12-02
AU2013346504A1 (en) 2015-06-18
IN2015DN04148A (pt) 2015-10-16
WO2014076571A3 (en) 2014-11-06
WO2014076571A9 (en) 2015-04-02
WO2014076571A2 (en) 2014-05-22
EP2920310A2 (en) 2015-09-23
US20160272980A1 (en) 2016-09-22

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B06F Objections, documents and/or translations needed after an examination request according [chapter 6.6 patent gazette]
B06F Objections, documents and/or translations needed after an examination request according [chapter 6.6 patent gazette]
B06I Publication of requirement cancelled [chapter 6.9 patent gazette]

Free format text: ANULADA A PUBLICACAO CODIGO 6.6.1 NA RPI NO 2462 DE 13/03/2018 POR TER SIDO INDEVIDA.

B06U Preliminary requirement: requests with searches performed by other patent offices: procedure suspended [chapter 6.21 patent gazette]
B11B Dismissal acc. art. 36, par 1 of ipl - no reply within 90 days to fullfil the necessary requirements
B350 Update of information on the portal [chapter 15.35 patent gazette]