AU2013217130B2 - Production of beta-phellandrene using genetically engineered cyanobacteria - Google Patents

Production of beta-phellandrene using genetically engineered cyanobacteria Download PDF

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Publication number
AU2013217130B2
AU2013217130B2 AU2013217130A AU2013217130A AU2013217130B2 AU 2013217130 B2 AU2013217130 B2 AU 2013217130B2 AU 2013217130 A AU2013217130 A AU 2013217130A AU 2013217130 A AU2013217130 A AU 2013217130A AU 2013217130 B2 AU2013217130 B2 AU 2013217130B2
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Prior art keywords
phellandrene
chens
cyanobacteria
phls
pct
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AU2013217130A
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AU2013217130A1 (en
Inventor
Fiona K. BENTLEY
Anastasios Melis
Hsu-Ching Chen Wintz
Andreas Zurbriggen
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University of California San Diego UCSD
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University of California Berkeley
University of California San Diego UCSD
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P5/00—Preparation of hydrocarbons or halogenated hydrocarbons
    • C12P5/002—Preparation of hydrocarbons or halogenated hydrocarbons cyclic
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/20—Bacteria; Culture media therefor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/52—Genes encoding for enzymes or proenzymes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/88—Lyases (4.)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P5/00—Preparation of hydrocarbons or halogenated hydrocarbons
    • C12P5/007—Preparation of hydrocarbons or halogenated hydrocarbons containing one or more isoprene units, i.e. terpenes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y402/00—Carbon-oxygen lyases (4.2)
    • C12Y402/03—Carbon-oxygen lyases (4.2) acting on phosphates (4.2.3)
    • C12Y402/03052—(4S)-Beta-phellandrene synthase (geranyl-diphosphate-cyclizing) (4.2.3.52)

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  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Health & Medical Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Genetics & Genomics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Virology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
AU2013217130A 2012-02-06 2013-02-06 Production of beta-phellandrene using genetically engineered cyanobacteria Active AU2013217130B2 (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US201261595610P 2012-02-06 2012-02-06
US61/595,610 2012-02-06
PCT/US2013/024908 WO2013119644A1 (en) 2012-02-06 2013-02-06 Production of beta-phellandrene using genetically engineered cyanobacteria

Publications (2)

Publication Number Publication Date
AU2013217130A1 AU2013217130A1 (en) 2014-08-21
AU2013217130B2 true AU2013217130B2 (en) 2018-10-11

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Family Applications (1)

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AU2013217130A Active AU2013217130B2 (en) 2012-02-06 2013-02-06 Production of beta-phellandrene using genetically engineered cyanobacteria

Country Status (4)

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US (3) US9951354B2 (GUID-C5D7CC26-194C-43D0-91A1-9AE8C70A9BFF.html)
AU (1) AU2013217130B2 (GUID-C5D7CC26-194C-43D0-91A1-9AE8C70A9BFF.html)
IN (1) IN2014DN07103A (GUID-C5D7CC26-194C-43D0-91A1-9AE8C70A9BFF.html)
WO (1) WO2013119644A1 (GUID-C5D7CC26-194C-43D0-91A1-9AE8C70A9BFF.html)

Families Citing this family (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US9951354B2 (en) 2012-02-06 2018-04-24 The Regents Of The University Of California Production of β-phellandrene using genetically engineered cyanobacteria
JP2014076042A (ja) * 2012-09-21 2014-05-01 Sekisui Chem Co Ltd 組換え細胞、並びに、β−フェランドレンの生産方法
WO2016008883A1 (en) * 2014-07-14 2016-01-21 Photanol B.V. Biosynthesis of monoterpenes in cyanobacteria
AU2016281666B2 (en) 2015-06-26 2022-08-25 The Regents Of The University Of California Fusion constructs as protein over-expression vectors
EP3464584A4 (en) * 2016-05-27 2020-07-29 The Regents of the University of California PRODUCTION OF MIXTURES OF MONOTERPENES BY UNICELLULAR PHOTOSYNTHETIC MICRO-ORGANISMS

Family Cites Families (12)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6818424B2 (en) * 2000-09-01 2004-11-16 E. I. Du Pont De Nemours And Company Production of cyclic terpenoids
US20020164706A1 (en) * 2001-01-30 2002-11-07 Huang Lisa L. High level promoters from cyanobacteria
US20090053797A1 (en) * 2005-08-19 2009-02-26 Yoichiro Shiba Genetically modified host cells and use of same for producing isoprenoid compounds
CN101617039A (zh) * 2006-10-20 2009-12-30 代表亚利桑那大学的亚利桑那校董会 经修饰的蓝细菌
US8609385B2 (en) * 2007-05-01 2013-12-17 Zuvachem, Inc. Methods for the direct conversion of carbon dioxide into a hydrocarbon using a metabolically engineered photosynthetic microorganism
EP2765198A3 (en) * 2007-09-11 2014-12-03 Sapphire Energy, Inc. Isoprenoid production by genetically modified chloroplasts
CA2728285A1 (en) * 2008-03-03 2009-09-11 Joule Unlimited, Inc. Engineered co2 fixing microorganisms producing carbon-based products of interest
WO2010127290A2 (en) * 2009-05-01 2010-11-04 The Regents Of The University Of California Improved isoprene hydrocarbon production using genetically engineered cyanobacteria
US8993303B2 (en) * 2011-02-24 2015-03-31 South Dakota State University Genetically engineered cyanobacteria
AU2012245238B2 (en) 2011-04-21 2015-11-26 The Regents Of The University Of California Diffusion-based method for obtaining volatile hydrocarbons produced by photosynthetic microorganisms in two-phase bioreactors
US9951354B2 (en) * 2012-02-06 2018-04-24 The Regents Of The University Of California Production of β-phellandrene using genetically engineered cyanobacteria
JP2014076042A (ja) * 2012-09-21 2014-05-01 Sekisui Chem Co Ltd 組換え細胞、並びに、β−フェランドレンの生産方法

Non-Patent Citations (2)

* Cited by examiner, † Cited by third party
Title
Demissie ZA et al, "Cloning and functional characterization of ß-phellandrene synthase from Lavandula angustifolia", Planta, 2011, 233(4):685-696 *
Reiling KK et al, "Mono and Diterpene Production in Escherichia coli", Biotechnology and Bioengineering, 2004, 87(2):200-212. *

Also Published As

Publication number Publication date
WO2013119644A1 (en) 2013-08-15
IN2014DN07103A (GUID-C5D7CC26-194C-43D0-91A1-9AE8C70A9BFF.html) 2015-04-24
US20200208178A1 (en) 2020-07-02
US20140370562A1 (en) 2014-12-18
US9951354B2 (en) 2018-04-24
US10563228B2 (en) 2020-02-18
US20180171363A1 (en) 2018-06-21
AU2013217130A1 (en) 2014-08-21

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