AU2012335087B2 - Polypeptide substrate for the detection of von Willebrand factor cleaving protease ADAMTS13 - Google Patents

Polypeptide substrate for the detection of von Willebrand factor cleaving protease ADAMTS13 Download PDF

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Publication number
AU2012335087B2
AU2012335087B2 AU2012335087A AU2012335087A AU2012335087B2 AU 2012335087 B2 AU2012335087 B2 AU 2012335087B2 AU 2012335087 A AU2012335087 A AU 2012335087A AU 2012335087 A AU2012335087 A AU 2012335087A AU 2012335087 B2 AU2012335087 B2 AU 2012335087B2
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Prior art keywords
adamts13
seq
polypeptide substrate
amino acid
substrate
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AU2012335087A
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AU2012335087A1 (en
Inventor
Suzette C. CHANCE
Gian Paolo Visentin
Elizabeth WUITSCHICK
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Immucor GTI Diagnostics Inc
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Immucor GTI Diagnostics Inc
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/34—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving hydrolase
    • C12Q1/37—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving hydrolase involving peptidase or proteinase
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/745—Blood coagulation or fibrinolysis factors
    • C07K14/755—Factors VIII, e.g. factor VIII C (AHF), factor VIII Ag (VWF)
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/573—Immunoassay; Biospecific binding assay; Materials therefor for enzymes or isoenzymes
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/60—Fusion polypeptide containing spectroscopic/fluorescent detection, e.g. green fluorescent protein [GFP]
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2333/00—Assays involving biological materials from specific organisms or of a specific nature
    • G01N2333/435—Assays involving biological materials from specific organisms or of a specific nature from animals; from humans
    • G01N2333/745—Assays involving non-enzymic blood coagulation factors
    • G01N2333/755—Factors VIII, e.g. factor VIII C [AHF], factor VIII Ag [VWF]
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2333/00—Assays involving biological materials from specific organisms or of a specific nature
    • G01N2333/90—Enzymes; Proenzymes
    • G01N2333/914—Hydrolases (3)
    • G01N2333/948—Hydrolases (3) acting on peptide bonds (3.4)
    • G01N2333/95—Proteinases, i.e. endopeptidases (3.4.21-3.4.99)
    • G01N2333/964—Proteinases, i.e. endopeptidases (3.4.21-3.4.99) derived from animal tissue
    • G01N2333/96425—Proteinases, i.e. endopeptidases (3.4.21-3.4.99) derived from animal tissue from mammals
    • G01N2333/96427—Proteinases, i.e. endopeptidases (3.4.21-3.4.99) derived from animal tissue from mammals in general
    • G01N2333/9643—Proteinases, i.e. endopeptidases (3.4.21-3.4.99) derived from animal tissue from mammals in general with EC number
    • G01N2333/96486—Metalloendopeptidases (3.4.24)

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  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Zoology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Immunology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Hematology (AREA)
  • Genetics & Genomics (AREA)
  • Biophysics (AREA)
  • Wood Science & Technology (AREA)
  • Medicinal Chemistry (AREA)
  • Biotechnology (AREA)
  • Physics & Mathematics (AREA)
  • Microbiology (AREA)
  • Analytical Chemistry (AREA)
  • Biomedical Technology (AREA)
  • Urology & Nephrology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Toxicology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Food Science & Technology (AREA)
  • Cell Biology (AREA)
  • Pathology (AREA)
  • General Physics & Mathematics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Peptides Or Proteins (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Enzymes And Modification Thereof (AREA)
AU2012335087A 2011-11-11 2012-11-09 Polypeptide substrate for the detection of von Willebrand factor cleaving protease ADAMTS13 Active AU2012335087B2 (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US201161558927P 2011-11-11 2011-11-11
US61/558,927 2011-11-11
PCT/US2012/064526 WO2013071168A1 (en) 2011-11-11 2012-11-09 Polypeptide substrate for the detection of von willebrand factor cleaving protease adamts13

Publications (2)

Publication Number Publication Date
AU2012335087A1 AU2012335087A1 (en) 2014-06-19
AU2012335087B2 true AU2012335087B2 (en) 2017-06-29

Family

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Family Applications (1)

Application Number Title Priority Date Filing Date
AU2012335087A Active AU2012335087B2 (en) 2011-11-11 2012-11-09 Polypeptide substrate for the detection of von Willebrand factor cleaving protease ADAMTS13

Country Status (6)

Country Link
US (3) US9513290B2 (enExample)
EP (1) EP2776461B1 (enExample)
JP (1) JP6419576B2 (enExample)
AU (1) AU2012335087B2 (enExample)
CA (1) CA2855498A1 (enExample)
WO (1) WO2013071168A1 (enExample)

Families Citing this family (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP7002727B2 (ja) * 2016-01-08 2022-02-15 Ｋｍバイオロジクス株式会社 Ａｄａｍｔｓ１３を主成分とする診断薬および医薬品
KR102386584B1 (ko) 2016-10-11 2022-04-14 래버러토리 코포레이션 오브 아메리카 홀딩스 Adamts13 효소 활성을 결정하기 위한 방법 및 시스템
CN106596936B (zh) * 2016-12-19 2019-05-31 吴江华药生物技术有限公司 体外定量测定vWF-CP酶活性的方法、检测试剂盒及制备方法
CN111153962B (zh) * 2020-01-15 2021-09-28 苏州智影特生物医药技术有限公司 一种血栓性血小板减少性紫癜病诊断探针及其制备方法和应用
EP4279607A1 (de) 2022-05-20 2023-11-22 Siemens Healthcare Diagnostics Products GmbH Enzym-verstärkter adamts-13 aktivitätstest
WO2024153750A1 (en) * 2023-01-18 2024-07-25 Enzyre B.V. Luminescent detection of adamts13

Family Cites Families (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5556771A (en) 1995-02-10 1996-09-17 Gen-Probe Incorporated Stabilized compositions of reverse transcriptase and RNA polymerase for nucleic acid amplification
US8663912B2 (en) * 2011-07-18 2014-03-04 Washington University Fluorogenic substrate for ADAMTS13

Non-Patent Citations (4)

* Cited by examiner, † Cited by third party
Title
DAYANANDA K. M. ET AL: "Escherichia coli-derived von Willebrand factor-A2 domainfluorescence/Frster resonance energy transfer proteins that quantify ADAMTS13activity", ANALYTICAL BIOCHEMISTRY, vol. 410, no. 2, 6 December 2010, pages 206-213 *
GAO W. et al., "Extensive contacts between ADAMTS13 exosites and von Willebrand factor domain A2 contribute to substrate specificity", Blood. 2008 Sep 1; 112(5): 1713-1719 *
KOKAME K. ET AL: "FRETS-VWF73, a first fluorogenic substrate for ADAMTS13 assay" BRITISH JOURNAL OF HAEMATOLOGY, vol. 129, no. l, 14 March 2005, pages 93-100 *
RAIFE T. J. et al., "Leukocyte proteases cleave vonWillebrand factor at or near the ADAMTS 13 cleavage site", Blood, 2009, 114(8): 1666-1674 *

Also Published As

Publication number Publication date
CA2855498A1 (en) 2013-05-16
JP2014533115A (ja) 2014-12-11
US20190185905A1 (en) 2019-06-20
EP2776461B1 (en) 2018-02-28
US20170145471A1 (en) 2017-05-25
US20150147764A1 (en) 2015-05-28
EP2776461A1 (en) 2014-09-17
US10538802B2 (en) 2020-01-21
JP6419576B2 (ja) 2018-11-07
WO2013071168A1 (en) 2013-05-16
US10202633B2 (en) 2019-02-12
US9513290B2 (en) 2016-12-06
AU2012335087A1 (en) 2014-06-19

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Owner name: IMMUCOR GTI DIAGNOSTICS, INC.

Free format text: FORMER APPLICANT(S): GEN-PROBE GTI DIAGNOSTICS, INC. C/O GEN-PROBE INCORPORATED

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