AU2012315852B2 - Sequences and their use for detection and characterization of STEC bacteria - Google Patents

Sequences and their use for detection and characterization of STEC bacteria Download PDF

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Publication number
AU2012315852B2
AU2012315852B2 AU2012315852A AU2012315852A AU2012315852B2 AU 2012315852 B2 AU2012315852 B2 AU 2012315852B2 AU 2012315852 A AU2012315852 A AU 2012315852A AU 2012315852 A AU2012315852 A AU 2012315852A AU 2012315852 B2 AU2012315852 B2 AU 2012315852B2
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AU
Australia
Prior art keywords
primer
probe
sequence
dna
quencher
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Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
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AU2012315852A
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English (en)
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AU2012315852A1 (en
Inventor
Daniel R. Demarco
Mark A. Jensen
Stephen Varkey
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Qualicon Diagnostics LLC
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Qualicon Diagnostics LLC
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Application filed by Qualicon Diagnostics LLC filed Critical Qualicon Diagnostics LLC
Publication of AU2012315852A1 publication Critical patent/AU2012315852A1/en
Assigned to QUALICON DIAGNOSTICS, LLC reassignment QUALICON DIAGNOSTICS, LLC Request for Assignment Assignors: E.I. DUPONT DE NEMOURS AND COMPANY
Application granted granted Critical
Publication of AU2012315852B2 publication Critical patent/AU2012315852B2/en
Ceased legal-status Critical Current
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • C12Q1/6888—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms
    • C12Q1/689—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms for bacteria

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  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Analytical Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Immunology (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
AU2012315852A 2011-09-28 2012-09-28 Sequences and their use for detection and characterization of STEC bacteria Ceased AU2012315852B2 (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US201161540119P 2011-09-28 2011-09-28
US61/540,119 2011-09-28
PCT/US2012/057795 WO2013049489A1 (en) 2011-09-28 2012-09-28 Sequences and their use for detection and characterization of stec bacteria

Publications (2)

Publication Number Publication Date
AU2012315852A1 AU2012315852A1 (en) 2014-03-06
AU2012315852B2 true AU2012315852B2 (en) 2018-03-01

Family

ID=47016845

Family Applications (1)

Application Number Title Priority Date Filing Date
AU2012315852A Ceased AU2012315852B2 (en) 2011-09-28 2012-09-28 Sequences and their use for detection and characterization of STEC bacteria

Country Status (7)

Country Link
US (2) US8802371B2 (https=)
EP (1) EP2761023B1 (https=)
JP (1) JP2014530011A (https=)
CN (1) CN103890192A (https=)
AU (1) AU2012315852B2 (https=)
BR (1) BR112014007394A2 (https=)
WO (1) WO2013049489A1 (https=)

Families Citing this family (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20140005061A1 (en) * 2012-06-29 2014-01-02 Life Technologies Corporation Compositions and methods for detection of multiple microorganisms
US10190177B2 (en) * 2014-08-01 2019-01-29 The Curators Of The University Of Missouri Multiplex assay for detection of bacterial species in biological samples
CN104593484A (zh) * 2014-12-04 2015-05-06 湖北永邦医疗科技有限公司 一种用于检测志贺毒素的引物、探针和试剂盒
KR101649851B1 (ko) * 2014-12-30 2016-08-30 주식회사 인트론바이오테크놀로지 신규한 시가독소생산 F18형 대장균 박테리오파지 Ｅｓｃ-ＣＯＰ-1 및 이의 시가독소생산 F18형 대장균 증식 억제 용도
US20200087709A1 (en) * 2018-03-13 2020-03-19 Microinvestigate, Llc Direct-to-consumer genomic diagnostic device
WO2024161179A1 (en) * 2023-01-31 2024-08-08 Mobidiag Oy Compositions and methods for detecting stx nucleic acids
CN116903710B (zh) * 2023-09-12 2023-11-14 山东省农业科学院畜牧兽医研究所 噬菌体靶向蛋白分子及其在鉴定o103抗原血清型的产志贺毒素大肠杆菌中的应用
CN117024531B (zh) * 2023-10-10 2023-12-12 山东省农业科学院畜牧兽医研究所 噬菌体靶向蛋白分子及其在鉴定o111抗原血清型的产志贺毒素大肠杆菌中的应用

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1998048046A2 (en) * 1997-04-22 1998-10-29 Bavarian Nordic Research Institute A/S Taqmantm-pcr for the detection of pathogenic e. coli strains
WO2005005659A1 (en) * 2003-07-14 2005-01-20 Statens Serum Institut Diagnostics of diarrheagenic escherichia coli (dec) and shigella spp.
EP2292799A1 (en) * 2009-08-11 2011-03-09 Agence Française de Securité Sanitaire des Aliments An assay for determining a molecular risk assessment of stec isolates

Family Cites Families (15)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4683195A (en) 1986-01-30 1987-07-28 Cetus Corporation Process for amplifying, detecting, and/or-cloning nucleic acid sequences
US4683202A (en) 1985-03-28 1987-07-28 Cetus Corporation Process for amplifying nucleic acid sequences
US4762857A (en) 1986-05-30 1988-08-09 E. I. Du Pont De Nemours And Company Trehalose as stabilizer and tableting excipient
US4678812A (en) 1986-05-30 1987-07-07 E. I. Du Pont De Nemours And Company Trehalose as stabilizer and tableting excipient
CA1323293C (en) 1987-12-11 1993-10-19 Keith C. Backman Assay using template-dependent nucleic acid probe reorganization
US5210015A (en) 1990-08-06 1993-05-11 Hoffman-La Roche Inc. Homogeneous assay system using the nuclease activity of a nucleic acid polymerase
US5994056A (en) 1991-05-02 1999-11-30 Roche Molecular Systems, Inc. Homogeneous methods for nucleic acid amplification and detection
US5538848A (en) 1994-11-16 1996-07-23 Applied Biosystems Division, Perkin-Elmer Corp. Method for detecting nucleic acid amplification using self-quenching fluorescence probe
ATE186750T1 (de) 1995-09-20 1999-12-15 Du Pont Eine kontrolle für die pcr
US6312930B1 (en) 1996-09-16 2001-11-06 E. I. Du Pont De Nemours And Company Method for detecting bacteria using PCR
GB9812768D0 (en) 1998-06-13 1998-08-12 Zeneca Ltd Methods
EP1185697A2 (en) 1999-04-30 2002-03-13 Qualicon, Inc. Assay based on the analysis of dna melting curves
JP4501443B2 (ja) * 2004-02-03 2010-07-14 東ソー株式会社 腸管出血性大腸菌の志賀毒素群遺伝子の検出試薬
CN100489527C (zh) * 2006-02-28 2009-05-20 中华人民共和国上海出入境检验检疫局 产志贺毒素大肠杆菌核酸纯化血清鉴定试剂盒
MX2012000969A (es) * 2009-07-22 2012-02-28 Du Pont Secuencias y su uso para deteccion y caracterizacion de o157:h7 de e.coli.

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1998048046A2 (en) * 1997-04-22 1998-10-29 Bavarian Nordic Research Institute A/S Taqmantm-pcr for the detection of pathogenic e. coli strains
WO2005005659A1 (en) * 2003-07-14 2005-01-20 Statens Serum Institut Diagnostics of diarrheagenic escherichia coli (dec) and shigella spp.
EP2292799A1 (en) * 2009-08-11 2011-03-09 Agence Française de Securité Sanitaire des Aliments An assay for determining a molecular risk assessment of stec isolates

Also Published As

Publication number Publication date
US9528162B2 (en) 2016-12-27
AU2012315852A1 (en) 2014-03-06
WO2013049489A1 (en) 2013-04-04
US8802371B2 (en) 2014-08-12
BR112014007394A2 (pt) 2018-08-28
CN103890192A (zh) 2014-06-25
JP2014530011A (ja) 2014-11-17
US20130078634A1 (en) 2013-03-28
US20140323710A1 (en) 2014-10-30
EP2761023B1 (en) 2018-03-07
EP2761023A1 (en) 2014-08-06

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PC1 Assignment before grant (sect. 113)

Owner name: QUALICON DIAGNOSTICS, LLC

Free format text: FORMER APPLICANT(S): E.I. DUPONT DE NEMOURS AND COMPANY

FGA Letters patent sealed or granted (standard patent)
MK14 Patent ceased section 143(a) (annual fees not paid) or expired