AU2012222144B2 - Genetically modified animals and methods for making the same - Google Patents

Genetically modified animals and methods for making the same Download PDF

Info

Publication number
AU2012222144B2
AU2012222144B2 AU2012222144A AU2012222144A AU2012222144B2 AU 2012222144 B2 AU2012222144 B2 AU 2012222144B2 AU 2012222144 A AU2012222144 A AU 2012222144A AU 2012222144 A AU2012222144 A AU 2012222144A AU 2012222144 B2 AU2012222144 B2 AU 2012222144B2
Authority
AU
Australia
Prior art keywords
cell
talen
embryo
bovine
allele
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Active
Application number
AU2012222144A
Other languages
English (en)
Other versions
AU2012222144A1 (en
Inventor
Daniel F. Carlson
Scott C. Fahrenkrug
Simon Geoffrey Lillico
Christopher James Palgrave
Christopher Bruce Alexander Whitelaw
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
University of Edinburgh
Recombinetics Inc
Original Assignee
University of Edinburgh
Recombinetics Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by University of Edinburgh, Recombinetics Inc filed Critical University of Edinburgh
Publication of AU2012222144A1 publication Critical patent/AU2012222144A1/en
Priority to AU2017202379A priority Critical patent/AU2017202379B2/en
Application granted granted Critical
Publication of AU2012222144B2 publication Critical patent/AU2012222144B2/en
Priority to AU2019264610A priority patent/AU2019264610A1/en
Active legal-status Critical Current
Anticipated expiration legal-status Critical

Links

Classifications

    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K67/00—Rearing or breeding animals, not otherwise provided for; New or modified breeds of animals
    • A01K67/027—New or modified breeds of vertebrates
    • A01K67/0275—Genetically modified vertebrates, e.g. transgenic
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K67/00—Rearing or breeding animals, not otherwise provided for; New or modified breeds of animals
    • A01K67/027—New or modified breeds of vertebrates
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K67/00—Rearing or breeding animals, not otherwise provided for; New or modified breeds of animals
    • A01K67/027—New or modified breeds of vertebrates
    • A01K67/0275—Genetically modified vertebrates, e.g. transgenic
    • A01K67/0276—Knock-out vertebrates
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61D—VETERINARY INSTRUMENTS, IMPLEMENTS, TOOLS, OR METHODS
    • A61D19/00—Instruments or methods for reproduction or fertilisation
    • A61D19/04—Instruments or methods for reproduction or fertilisation for embryo transplantation
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
    • C12N15/8509—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells for producing genetically modified animals, e.g. transgenic
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
    • C12N15/90—Stable introduction of foreign DNA into chromosome
    • C12N15/902—Stable introduction of foreign DNA into chromosome using homologous recombination
    • C12N15/907—Stable introduction of foreign DNA into chromosome using homologous recombination in mammalian cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/10—Cells modified by introduction of foreign genetic material
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K2217/00—Genetically modified animals
    • A01K2217/07—Animals genetically altered by homologous recombination
    • A01K2217/072—Animals genetically altered by homologous recombination maintaining or altering function, i.e. knock in
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K2217/00—Genetically modified animals
    • A01K2217/07—Animals genetically altered by homologous recombination
    • A01K2217/075—Animals genetically altered by homologous recombination inducing loss of function, i.e. knock out
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K2227/00—Animals characterised by species
    • A01K2227/10—Mammal
    • A01K2227/101—Bovine
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K2227/00—Animals characterised by species
    • A01K2227/10—Mammal
    • A01K2227/108—Swine

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Biotechnology (AREA)
  • Wood Science & Technology (AREA)
  • Chemical & Material Sciences (AREA)
  • Biomedical Technology (AREA)
  • Organic Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Veterinary Medicine (AREA)
  • Environmental Sciences (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • Animal Behavior & Ethology (AREA)
  • Physics & Mathematics (AREA)
  • Plant Pathology (AREA)
  • Biophysics (AREA)
  • Biodiversity & Conservation Biology (AREA)
  • Animal Husbandry (AREA)
  • Cell Biology (AREA)
  • Mycology (AREA)
  • Transplantation (AREA)
  • Reproductive Health (AREA)
  • Public Health (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
AU2012222144A 2011-02-25 2012-02-24 Genetically modified animals and methods for making the same Active AU2012222144B2 (en)

Priority Applications (2)

Application Number Priority Date Filing Date Title
AU2017202379A AU2017202379B2 (en) 2011-02-25 2017-04-11 Gene edited livestock animals and methods of making the same
AU2019264610A AU2019264610A1 (en) 2011-02-25 2019-11-14 Genetically modified animals and methods for making the same

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US201161446651P 2011-02-25 2011-02-25
US61/446,651 2011-02-25
PCT/US2012/026500 WO2012116274A2 (en) 2011-02-25 2012-02-24 Genetically modified animals and methods for making the same

Related Child Applications (1)

Application Number Title Priority Date Filing Date
AU2017202379A Division AU2017202379B2 (en) 2011-02-25 2017-04-11 Gene edited livestock animals and methods of making the same

Publications (2)

Publication Number Publication Date
AU2012222144A1 AU2012222144A1 (en) 2013-10-10
AU2012222144B2 true AU2012222144B2 (en) 2017-04-27

Family

ID=46719944

Family Applications (3)

Application Number Title Priority Date Filing Date
AU2012222144A Active AU2012222144B2 (en) 2011-02-25 2012-02-24 Genetically modified animals and methods for making the same
AU2017202379A Active AU2017202379B2 (en) 2011-02-25 2017-04-11 Gene edited livestock animals and methods of making the same
AU2019264610A Abandoned AU2019264610A1 (en) 2011-02-25 2019-11-14 Genetically modified animals and methods for making the same

Family Applications After (2)

Application Number Title Priority Date Filing Date
AU2017202379A Active AU2017202379B2 (en) 2011-02-25 2017-04-11 Gene edited livestock animals and methods of making the same
AU2019264610A Abandoned AU2019264610A1 (en) 2011-02-25 2019-11-14 Genetically modified animals and methods for making the same

Country Status (13)

Country Link
US (3) US20120222143A1 (cg-RX-API-DMAC10.html)
EP (3) EP3461898A1 (cg-RX-API-DMAC10.html)
JP (4) JP2014509195A (cg-RX-API-DMAC10.html)
KR (1) KR20140046408A (cg-RX-API-DMAC10.html)
CN (4) CN106417184A (cg-RX-API-DMAC10.html)
AR (1) AR085507A1 (cg-RX-API-DMAC10.html)
AU (3) AU2012222144B2 (cg-RX-API-DMAC10.html)
BR (1) BR112013021785A8 (cg-RX-API-DMAC10.html)
CA (1) CA2828239C (cg-RX-API-DMAC10.html)
CL (1) CL2013002438A1 (cg-RX-API-DMAC10.html)
EA (1) EA201391234A1 (cg-RX-API-DMAC10.html)
MX (2) MX363013B (cg-RX-API-DMAC10.html)
WO (1) WO2012116274A2 (cg-RX-API-DMAC10.html)

Families Citing this family (75)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
HUE041426T2 (hu) 2009-11-02 2019-05-28 Univ Washington Terápiás nukleáz-készítmények és eljárások
US20110197290A1 (en) * 2010-02-11 2011-08-11 Fahrenkrug Scott C Methods and materials for producing transgenic artiodactyls
US9528124B2 (en) * 2013-08-27 2016-12-27 Recombinetics, Inc. Efficient non-meiotic allele introgression
US10920242B2 (en) 2011-02-25 2021-02-16 Recombinetics, Inc. Non-meiotic allele introgression
US20140201857A1 (en) * 2013-01-14 2014-07-17 Recombinetics, Inc. Hornless livestock
US20140123330A1 (en) * 2012-10-30 2014-05-01 Recombinetics, Inc. Control of sexual maturation in animals
CA2834626A1 (en) 2011-04-29 2012-11-01 University Of Washington Therapeutic nuclease compositions and methods
US8497124B2 (en) * 2011-12-05 2013-07-30 Factor Bioscience Inc. Methods and products for reprogramming cells to a less differentiated state
EP2802872A4 (en) * 2012-01-13 2015-09-09 Univ Leland Stanford Junior MECHANICAL BIOMARKERS FOR THE VITABILITY OF OOCYTES AND EMBRYOS
AU2013204327B2 (en) * 2012-04-20 2016-09-01 Aviagen Cell transfection method
SG10201806573TA (en) 2012-05-25 2018-09-27 Cellectis Methods for engineering allogeneic and immunosuppressive resistant t cell for immunotherapy
AR091482A1 (es) * 2012-06-21 2015-02-04 Recombinetics Inc Celulas modificadas geneticamente y metodos par su obtencion
WO2013191769A1 (en) * 2012-06-22 2013-12-27 Mayo Foundation For Medical Education And Research Genome editing
US10058078B2 (en) 2012-07-31 2018-08-28 Recombinetics, Inc. Production of FMDV-resistant livestock by allele substitution
GB201216564D0 (en) 2012-09-17 2012-10-31 Univ Edinburgh Genetically edited animal
CA2890110C (en) * 2012-11-01 2023-05-02 Factor Bioscience Inc. Methods and products for expressing proteins in cells
CN102964431B (zh) * 2012-12-03 2014-05-07 中国农业科学院北京畜牧兽医研究所 一对特异识别肌肉生长抑制素基因的多肽及其编码基因和应用
US9708589B2 (en) 2012-12-18 2017-07-18 Monsanto Technology Llc Compositions and methods for custom site-specific DNA recombinases
US10793867B2 (en) 2013-03-15 2020-10-06 Monsanto Technology, Llc Methods for targeted transgene-integration using custom site-specific DNA recombinases
US11039586B2 (en) 2013-03-15 2021-06-22 Monsanto Technology Llc Creation and transmission of megaloci
US10604771B2 (en) * 2013-05-10 2020-03-31 Sangamo Therapeutics, Inc. Delivery methods and compositions for nuclease-mediated genome engineering
US11077144B2 (en) 2013-05-13 2021-08-03 Cellectis CD19 specific chimeric antigen receptor and uses thereof
US20140359795A1 (en) * 2013-05-31 2014-12-04 Recombinetics, Inc. Genetic techniques for making animals with sortable sperm
US20140359796A1 (en) * 2013-05-31 2014-12-04 Recombinetics, Inc. Genetically sterile animals
EP3008181B1 (en) * 2013-06-11 2019-11-06 The Regents of The University of California Methods and compositions for target dna modification
US10124336B2 (en) 2013-08-16 2018-11-13 Massachusetts Institute Of Technology Selective delivery of material to cells
WO2015035034A1 (en) * 2013-09-04 2015-03-12 Mice With Horns, Llc Materials and methods for correcting recessive mutations in animals
DE102013111099B4 (de) 2013-10-08 2023-11-30 Eberhard Karls Universität Tübingen Medizinische Fakultät Permanente Genkorrektur mittels nukleotidmodifizierter messenger RNA
ES2962492T3 (es) 2013-10-25 2024-03-19 Livestock Improvement Corporation Ltd Marcadores genéticos y usos de los mismos
SI3063275T1 (sl) 2013-10-31 2020-02-28 Resolve Therapeutics, Llc Terapevtske fuzije nukleaza-albumin in postopki
KR101566498B1 (ko) * 2013-11-13 2015-11-06 건국대학교 산학협력단 인터루킨 2 수용체 감마 유전자 적중벡터, 그 벡터가 도입된 면역세포 결핍 형질전환 미니 복제돼지 생산과 그 제조방법 및 활용
ES2721936T3 (es) * 2013-11-13 2019-08-06 Childrens Medical Center Regulación de la expresión genética mediada por las nucleasas
KR101554166B1 (ko) * 2013-11-13 2015-09-22 건국대학교 산학협력단 재조합 활성 유전자 2 유전자 적중벡터, 그 벡터가 도입된 면역세포 결핍 형질전환 미니 복제돼지 생산과 그 제조방법 및 활용
CN103952424B (zh) * 2014-04-23 2017-01-11 尹熙俊 生产mstn双侧基因敲除的双肌性状体细胞克隆猪的方法
BR122023023211A2 (pt) * 2014-04-28 2024-01-23 Recombinetics, Inc. Método de fazer edições de genes multiplex em uma célula de vertebrado ou de embrião primário não-humano
CN103952405B (zh) * 2014-05-13 2016-02-10 扬州大学 一种山羊mstn基因定点修饰系统及其应用
AU2015320738B2 (en) 2014-09-23 2021-12-02 Acceligen, Inc. Materials and methods for producing animals with short hair
CN122564053A (zh) 2014-10-31 2026-08-14 麻省理工学院 递送生物分子至免疫细胞
CA2967027A1 (en) * 2014-11-12 2016-05-19 Recombinetics, Inc. Heterozygous modifications of tumor suppressor genes
CN104450673B (zh) * 2014-11-14 2017-07-21 中国农业大学 一种y染色体修饰方法及其应用
CN104388560B (zh) * 2014-11-14 2017-04-12 中国农业大学 一种y染色体标记方法及其应用
US20180105834A1 (en) * 2014-11-27 2018-04-19 Institute Of Animal Sciences, Chinese Academy Of Agrigultural Sciences A method of site-directed insertion to h11 locus in pigs by using site-directed cutting system
KR101724805B1 (ko) * 2014-12-16 2017-04-11 서울대학교산학협력단 유전자 적중 물질을 이용한 조류 유전자 편집방법 및 유전자 편집 조류
AU2016206870B2 (en) 2015-01-12 2022-02-17 Massachusetts Institute Of Technology Gene editing through microfluidic delivery
US10874092B2 (en) * 2015-06-30 2020-12-29 Regents Of The University Of Minnesota Humanized skeletal muscle
JP6925984B2 (ja) 2015-07-09 2021-08-25 マサチューセッツ インスティテュート オブ テクノロジー 無核細胞への物質の送達
US11339408B2 (en) 2015-08-20 2022-05-24 Applied Stemcell, Inc. Nuclease with enhanced efficiency of genome editing
US11613759B2 (en) 2015-09-04 2023-03-28 Sqz Biotechnologies Company Intracellular delivery of biomolecules to cells comprising a cell wall
EP3673732A3 (en) 2015-10-27 2020-07-29 Recombinetics, Inc. Engineering of humanized car t-cells and platelets by genetic complementation
EP4279084B1 (en) 2015-10-28 2025-06-11 Vertex Pharmaceuticals Inc. Materials and methods for treatment of duchenne muscular dystrophy
CN105543257A (zh) * 2016-01-18 2016-05-04 安徽农业大学 一种pAPN基因定点修饰猪
CA3027428A1 (en) * 2016-06-14 2017-12-21 Regents Of The University Of Minnesota Genetically modified cells, tissues, and organs for treating disease
CN109790551A (zh) * 2016-06-16 2019-05-21 奥斯陆大学医院Hf 改进的基因编辑
MA45554A (fr) 2016-07-01 2019-05-08 Resolve Therapeutics Llc Fusions de binucléase optimisées.
US20180084767A1 (en) 2016-09-21 2018-03-29 Recombinetics, Inc. Animal models for cardiomyopathy
GB201617559D0 (en) 2016-10-17 2016-11-30 University Court Of The University Of Edinburgh The Swine comprising modified cd163 and associated methods
EP3535394B1 (en) 2016-11-03 2023-04-12 Temple University - Of The Commonwealth System of Higher Education Dna plasmids for the fast generation of homologous recombination vectors for cell line development
US10457683B2 (en) 2017-04-12 2019-10-29 Magenta Therapeutics Inc. Aryl hydrocarbon receptor antagonists and uses thereof
CN108728477B (zh) * 2017-04-24 2022-02-22 华东理工大学 一种高效的转座突变系统及构建方法
IL310452A (en) * 2017-08-09 2024-03-01 Ricetec Inc Preparations and methods for genome modification
US20190045761A1 (en) 2017-08-11 2019-02-14 Recombinetics, Inc. Inducible disease models methods of making them and use in tissue complementation
CN111315767B (zh) 2017-08-22 2025-03-18 萨纳生物有限责任公司 可溶性干扰素受体及其用途
US20190314407A1 (en) 2017-10-31 2019-10-17 Magenta Therapeutics Inc. Compositions and methods for the expansion of hematopoietic stem and progenitor cells
IL275077B2 (en) 2017-12-06 2025-02-01 Magenta Therapeutics Inc Dosing regimens for mobilization of hematopoietic stem and progenitor cells.
CN108949830B (zh) * 2018-08-03 2021-11-26 福州大学 一种在鱼类中实现基因组编辑、精确定点基因敲入的方法
CN111793654B (zh) * 2019-04-08 2022-05-24 中国农业大学 一种提高CRISPR/Cas9介导的双等位基因突变效率的方法及其应用
IL287088B2 (en) * 2019-04-08 2023-09-01 Dna Twopointo Inc Transposition of nucleic acid constructs into eukaryotic genomes with amylase transposases
WO2021087406A1 (en) 2019-11-01 2021-05-06 Magenta Therapeutics, Inc. Dosing regimens for the mobilization of hematopoietic stem and progentor cells
AU2021263754A1 (en) 2020-04-27 2022-12-01 Ensoma, Inc. Methods and compositions for transducing hematopoietic stem and progenitor cells in vivo
WO2022197776A1 (en) 2021-03-16 2022-09-22 Magenta Therapeutics, Inc. Dosing regimens for hematopoietic stem cell mobilization for stem cell transplants in multiple myeloma patients
GB202118058D0 (en) 2021-12-14 2022-01-26 Univ Warwick Methods to increase yields in crops
US20250129385A1 (en) * 2022-02-01 2025-04-24 Abs Global, Inc. Gene-editing methods for embryonic stem cells
GB2621813A (en) 2022-06-30 2024-02-28 Univ Newcastle Preventing disease recurrence in Mitochondrial replacement therapy
WO2024036106A1 (en) * 2022-08-08 2024-02-15 The Board Of Trustees Of The Leland Stanford Junior University Embryo microglia complementation for in vivo microglia manipulation and production of a non-human animal model for validation of gene function and therapeutic screening
GB202314578D0 (en) 2023-09-22 2023-11-08 Univ Manchester Methods of producing homoplasmic modified plants or parts thereof

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2010143917A2 (en) * 2009-06-11 2010-12-16 Toolgen Incorporation Targeted genomic rearrangements using site-specific nucleases
WO2011019385A1 (en) * 2009-08-11 2011-02-17 Sangamo Biosciences, Inc. Organisms homozygous for targeted modification
WO2011072246A2 (en) * 2009-12-10 2011-06-16 Regents Of The University Of Minnesota Tal effector-mediated dna modification

Family Cites Families (44)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4873191A (en) 1981-06-12 1989-10-10 Ohio University Genetic transformation of zygotes
US4888274A (en) 1985-09-18 1989-12-19 Yale University RecA nucleoprotein filament and methods
US5731178A (en) 1990-03-21 1998-03-24 Behringwerke Aktiengesellschaft Attachment-elements for stimulation of eukaryotic expression systems
US5510473A (en) 1990-11-09 1996-04-23 The United States Of American As Represented By The Secretary Of Health And Human Services Cloning of the recA gene from thermus aquaticus YT-1
US5763240A (en) 1992-04-24 1998-06-09 Sri International In vivo homologous sequence targeting in eukaryotic cells
US5674992A (en) 1992-08-28 1997-10-07 Cornell Research Foundation, Inc. CDNA encoding a recA homolog in eukaryotes
US5610053A (en) 1993-04-07 1997-03-11 The United States Of America As Represented By The Department Of Health And Human Services DNA sequence which acts as a chromatin insulator element to protect expressed genes from cis-acting regulatory sequences in mammalian cells
US5731411A (en) 1993-07-12 1998-03-24 The United States Of America As Represented By The Department Of Health And Human Services Promotion of homologous DNA pairing by RecA-derived peptides
EP0821070A1 (en) 1996-07-22 1998-01-28 Carelli, Claude Marcel Henri Pit-1 gene polymorphism and trait selection in animals
US6037525A (en) 1996-08-01 2000-03-14 North Carolina State University Method for reducing expression variability of transgenes in plant cells
US5948653A (en) 1997-03-21 1999-09-07 Pati; Sushma Sequence alterations using homologous recombination
WO1999002667A1 (en) * 1997-07-14 1999-01-21 University Of Liege Mutations in the myostation gene cause double-muscling in mammals
US6541684B1 (en) 1998-02-13 2003-04-01 Trustees Of Columbia University In The City Of New York Nucleotide sequences encoding maize RAD51
US6388169B1 (en) 1998-06-08 2002-05-14 Pioneer Hi-Bred International, Inc. Maize orthologues of bacterial RecA proteins
US6774213B1 (en) 1998-07-24 2004-08-10 Alberto I. Roca Mutants of MAW motifs of RecA protein homologs, methods of making them, and their uses
WO2000011155A1 (en) 1998-08-19 2000-03-02 The Board Of Trustees Of The Leland Stanford Junior University Methods and compositions for genomic modification
AU6218899A (en) 1998-10-12 2000-05-01 Geron Bio-Med Limited Porcine oocytes with improved developmental competence
AU1128400A (en) 1998-10-22 2000-05-08 Medical College Of Georgia Institute, Inc. Long terminal repeat, enhancer, and insulator sequences for use in recombinant vectors
US6720478B1 (en) 1999-05-05 2004-04-13 Pioneer Hi-Bred International, Inc. RAD51-like polynucleotide and uses thereof
CA2372635A1 (en) * 1999-05-06 2000-11-16 Stem Cell Sciences Pty Ltd. A process of cell reprogramming through production of a heterokaryon
WO2001030965A2 (en) 1999-10-28 2001-05-03 The Board Of Trustees Of The Leland Stanford Junior University Methods of in vivo gene transfer using a sleeping beauty transposon system
US6686515B1 (en) 1999-11-23 2004-02-03 Maxygen, Inc. Homologous recombination in plants
WO2001061049A1 (en) 2000-02-18 2001-08-23 The Board Of Trustees Of The Leland Stanford Junior University Altered recombinases for genome modification
ES2394877T3 (es) 2000-08-14 2013-02-06 The Government Of The United States Of America As Represented By The Secretary Of The Department Of Health And Human Services Recombinación homóloga mejorada mediada por proteínas de recombinación de lambda
US7199281B2 (en) 2001-09-07 2007-04-03 The Regents Of The University Of California Method of generating a transgenic livestock animal
EP1504092B2 (en) * 2002-03-21 2014-06-25 Sangamo BioSciences, Inc. Methods and compositions for using zinc finger endonucleases to enhance homologous recombination
US7176007B2 (en) 2002-12-12 2007-02-13 Wisconsin Alumni Research Foundation RecA mutants
CA2512110A1 (en) 2002-12-31 2004-07-22 Mmi Genomics, Inc. Compositions, methods, and systems for inferring bovine breed
WO2004065581A2 (en) 2003-01-15 2004-08-05 Discovery Genomics, Inc. Transposon-insulator element delivery systems
US7985739B2 (en) 2003-06-04 2011-07-26 The Board Of Trustees Of The Leland Stanford Junior University Enhanced sleeping beauty transposon system and methods for using the same
BRPI0415468A (pt) 2003-10-24 2007-03-27 Mmi Genomics Inc métodos e sistemas para inferir caracterìsticas para controlar criação não de gado
US7420099B2 (en) * 2004-04-22 2008-09-02 Kirin Holdings Kabushiki Kaisha Transgenic animals and uses thereof
CA2585098C (en) * 2004-10-22 2018-12-18 Revivicor, Inc. Porcine genomic kappa and lambda light chain sequences
WO2008060510A2 (en) * 2006-11-13 2008-05-22 Sangamo Biosciences, Inc. Zinc finger nuclease for targeting the human glucocorticoid receptor locus
WO2010008562A2 (en) 2008-07-16 2010-01-21 Recombinetics Methods and materials for producing transgenic animals
WO2010008564A2 (en) 2008-07-16 2010-01-21 Recombinetics Plaice dna transposon system
JP5908725B2 (ja) * 2008-08-22 2016-04-26 サンガモ バイオサイエンシーズ， インコーポレイテッド 標的一本鎖開裂および標的組込みのための方法、並びに組成物
US20110023158A1 (en) * 2008-12-04 2011-01-27 Sigma-Aldrich Co. Bovine genome editing with zinc finger nucleases
US20110023140A1 (en) 2008-12-04 2011-01-27 Sigma-Aldrich Co. Rabbit genome editing with zinc finger nucleases
EP2206723A1 (en) * 2009-01-12 2010-07-14 Bonas, Ulla Modular DNA-binding domains
KR20120097483A (ko) * 2009-07-24 2012-09-04 시그마-알드리치 컴퍼니., 엘엘씨 게놈 편집을 위한 방법
NZ598457A (en) 2009-08-03 2014-06-27 Recombinetics Inc Methods and compositions for targeted gene modification
US20110197290A1 (en) 2010-02-11 2011-08-11 Fahrenkrug Scott C Methods and materials for producing transgenic artiodactyls
AU2011256838B2 (en) * 2010-05-17 2014-10-09 Sangamo Therapeutics, Inc. Novel DNA-binding proteins and uses thereof

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2010143917A2 (en) * 2009-06-11 2010-12-16 Toolgen Incorporation Targeted genomic rearrangements using site-specific nucleases
WO2011019385A1 (en) * 2009-08-11 2011-02-17 Sangamo Biosciences, Inc. Organisms homozygous for targeted modification
WO2011072246A2 (en) * 2009-12-10 2011-06-16 Regents Of The University Of Minnesota Tal effector-mediated dna modification

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
CHRISTIAN M et al, Genetics, 2010, 186: 757-761 *

Also Published As

Publication number Publication date
JP2017184744A (ja) 2017-10-12
CN106417184A (zh) 2017-02-22
CA2828239A1 (en) 2012-08-30
AU2012222144A1 (en) 2013-10-10
AU2019264610A1 (en) 2019-12-12
CL2013002438A1 (es) 2014-08-29
CN107090470B (zh) 2021-09-07
JP2017184743A (ja) 2017-10-12
AR085507A1 (es) 2013-10-09
EP2678434A2 (en) 2014-01-01
NZ717891A (en) 2018-02-23
CN103930550B (zh) 2017-02-15
EA201391234A1 (ru) 2014-05-30
US20120222143A1 (en) 2012-08-30
CN107058383A (zh) 2017-08-18
EP3450563A1 (en) 2019-03-06
US20140351965A1 (en) 2014-11-27
BR112013021785A2 (pt) 2016-11-29
AU2017202379A1 (en) 2017-04-27
AU2017202379B2 (en) 2019-08-15
EP3461898A1 (en) 2019-04-03
JP2014509195A (ja) 2014-04-17
NZ712483A (en) 2017-11-24
CN103930550A (zh) 2014-07-16
WO2012116274A3 (en) 2012-12-27
JP2020010692A (ja) 2020-01-23
MX363013B (es) 2019-03-04
CA2828239C (en) 2020-10-06
NZ615712A (en) 2016-04-29
EP2678434B1 (en) 2018-11-07
BR112013021785A8 (pt) 2018-07-03
WO2012116274A2 (en) 2012-08-30
US20130117870A1 (en) 2013-05-09
CN107090470A (zh) 2017-08-25
KR20140046408A (ko) 2014-04-18
EP2678434A4 (en) 2014-11-26
MX2019002482A (es) 2019-07-04
MX2013009725A (es) 2014-01-31

Similar Documents

Publication Publication Date Title
AU2017202379B2 (en) Gene edited livestock animals and methods of making the same
AU2013277214B2 (en) Genetically edited animals and methods for making the same
US20190335725A1 (en) Genetically sterile animals
US20170099813A1 (en) Method of generating sterile terminal sires in livestock and animals produced thereby
NZ712483B2 (en) Genetically modified animals and methods for making the same
NZ717891B2 (en) Genetically modified animals and methods for making the same
NZ615712B2 (en) Genetically modified animals and methods for making the same
HK1243127A1 (en) Genetically modified animals and methods for making the same
HK1233854A1 (en) Genetically modified animals and methods for making the same
HK1233854A (en) Genetically modified animals and methods for making the same
NZ715540B2 (en) Genetically sterile animals

Legal Events

Date Code Title Description
FGA Letters patent sealed or granted (standard patent)