AU2008334901A1 - Methods and nucleic acids for analyses of lung carcinoma - Google Patents
Methods and nucleic acids for analyses of lung carcinoma Download PDFInfo
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- AU2008334901A1 AU2008334901A1 AU2008334901A AU2008334901A AU2008334901A1 AU 2008334901 A1 AU2008334901 A1 AU 2008334901A1 AU 2008334901 A AU2008334901 A AU 2008334901A AU 2008334901 A AU2008334901 A AU 2008334901A AU 2008334901 A1 AU2008334901 A1 AU 2008334901A1
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- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
- C12Q1/6883—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for diseases caused by alterations of genetic material
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- C12Q2600/00—Oligonucleotides characterized by their use
- C12Q2600/154—Methylation markers
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| US5912264A (en) | 1997-03-03 | 1999-06-15 | Bristol-Myers Squibb Company | 6-halo-or nitrate-substituted paclitaxels |
| AU7829398A (en) | 1997-06-09 | 1998-12-30 | University Of Southern California | A cancer diagnostic method based upon dna methylation differences |
| DE19754482A1 (de) | 1997-11-27 | 1999-07-01 | Epigenomics Gmbh | Verfahren zur Herstellung komplexer DNA-Methylierungs-Fingerabdrücke |
| US7700324B1 (en) | 1998-11-03 | 2010-04-20 | The Johns Hopkins University School Of Medicine | Methylated CpG island amplification (MCA) |
| US5958773A (en) | 1998-12-17 | 1999-09-28 | Isis Pharmaceuticals Inc. | Antisense modulation of AKT-1 expression |
| US6331393B1 (en) | 1999-05-14 | 2001-12-18 | University Of Southern California | Process for high-throughput DNA methylation analysis |
| JP2005512499A (ja) * | 2000-09-01 | 2005-05-12 | エピゲノミクス アーゲー | 進行中の疾病または特定の疾病の発病素質の診断 |
| US20050235375A1 (en) * | 2001-06-22 | 2005-10-20 | Wenqiong Chen | Transcription factors of cereals |
| US7473767B2 (en) | 2001-07-03 | 2009-01-06 | The Institute For Systems Biology | Methods for detection and quantification of analytes in complex mixtures |
| DE10338308B4 (de) | 2003-08-15 | 2006-10-19 | Epigenomics Ag | Verfahren zum Nachweis von Cytosin-Methylierungen in DNA |
| EP2281902A1 (en) * | 2004-07-18 | 2011-02-09 | Epigenomics AG | Epigenetic methods and nucleic acids for the detection of breast cell proliferative disorders |
| BRPI0518734A2 (pt) * | 2004-11-30 | 2008-12-02 | Veridex Llc | prognàstico de cÂncer de pulmço |
| EP2284279A1 (en) * | 2004-12-02 | 2011-02-16 | Epigenomics AG | Methods and nucleic acids for the analysis of gene expression associated with the prognosis of prostate cell proliferative disorders |
| ES2533767T3 (es) * | 2005-04-15 | 2015-04-15 | Epigenomics Ag | Métodos para el análisis de trastornos proliferativos celulares |
| WO2006128140A2 (en) * | 2005-05-27 | 2006-11-30 | Dana-Farber Cancer Institute, Inc. | Gene methylation and expression |
| EP1991274A4 (en) * | 2006-01-20 | 2009-06-10 | Women S And Children S Health | METHOD OF TREATMENT, PROPHYLAXIS AND DIAGNOSIS OF BONE PATHOLOGIES |
| WO2007085497A2 (en) * | 2006-01-30 | 2007-08-02 | Epigenomics Ag | Markers for the prediction of outcome of anthracycline treatment |
| ATE508207T1 (de) * | 2006-02-28 | 2011-05-15 | Charite Universitaetsmedizin | Nachweis und qualitätskontrolle von regulator-t- zellen mittels dna-methylierungsanalyse des foxp3-gens |
| WO2008010975A2 (en) * | 2006-07-14 | 2008-01-24 | The Johns Hopkins University | Early detection and prognosis of colon cancers |
| ES2384071T3 (es) * | 2007-01-19 | 2012-06-29 | Epigenomics Ag | Métodos y ácidos nucleicos para análisis de trastornos proliferativos celulares |
| US20090203011A1 (en) * | 2007-01-19 | 2009-08-13 | Epigenomics Ag | Methods and nucleic acids for analyses of cell proliferative disorders |
| CA2676227A1 (en) * | 2007-02-12 | 2008-08-21 | The Johns Hopkins University | Early detection and prognosis of colon cancers |
| JP2008212009A (ja) * | 2007-02-28 | 2008-09-18 | Sysmex Corp | Ｄｎａメチル化検出用試料の調製方法 |
| WO2011091332A2 (en) * | 2010-01-22 | 2011-07-28 | Chromatin, Inc. | Novel centromeres and methods of using the same |
-
2008
- 2008-12-11 JP JP2010537317A patent/JP5694776B2/ja not_active Expired - Fee Related
- 2008-12-11 AU AU2008334901A patent/AU2008334901A1/en not_active Abandoned
- 2008-12-11 CA CA2708163A patent/CA2708163A1/en not_active Abandoned
- 2008-12-11 EP EP08860719A patent/EP2229456A2/en not_active Withdrawn
- 2008-12-11 US US12/747,584 patent/US20110003292A1/en not_active Abandoned
- 2008-12-11 WO PCT/EP2008/010549 patent/WO2009074328A2/en not_active Ceased
- 2008-12-11 EP EP10008955A patent/EP2302069A1/en not_active Withdrawn
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2015
- 2015-12-22 US US14/978,642 patent/US20160194722A1/en not_active Abandoned
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2017
- 2017-08-09 US US15/672,858 patent/US20180023144A1/en not_active Abandoned
Also Published As
| Publication number | Publication date |
|---|---|
| JP5694776B2 (ja) | 2015-04-01 |
| EP2302069A1 (en) | 2011-03-30 |
| US20180023144A1 (en) | 2018-01-25 |
| US20110003292A1 (en) | 2011-01-06 |
| WO2009074328A3 (en) | 2009-08-13 |
| CA2708163A1 (en) | 2009-06-18 |
| WO2009074328A2 (en) | 2009-06-18 |
| JP2011505812A (ja) | 2011-03-03 |
| EP2229456A2 (en) | 2010-09-22 |
| WO2009074328A8 (en) | 2009-12-03 |
| US20160194722A1 (en) | 2016-07-07 |
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