AU2008312352A1 - Electrochemical assay for the detection of enzymes - Google Patents

Electrochemical assay for the detection of enzymes Download PDF

Info

Publication number
AU2008312352A1
AU2008312352A1 AU2008312352A AU2008312352A AU2008312352A1 AU 2008312352 A1 AU2008312352 A1 AU 2008312352A1 AU 2008312352 A AU2008312352 A AU 2008312352A AU 2008312352 A AU2008312352 A AU 2008312352A AU 2008312352 A1 AU2008312352 A1 AU 2008312352A1
Authority
AU
Australia
Prior art keywords
electrode
kinase
eam
ligands
substrate
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Abandoned
Application number
AU2008312352A
Other languages
English (en)
Inventor
Michael J. Ahrens
Paul A. Bertin
Dimitra Georganopoulou
Harry B. Gray
Thomas J. Meade
Markus Franz Wunder
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
OHMX Corp
Original Assignee
OHMX Corp
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by OHMX Corp filed Critical OHMX Corp
Publication of AU2008312352A1 publication Critical patent/AU2008312352A1/en
Abandoned legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/001—Enzyme electrodes
    • C12Q1/002—Electrode membranes
    • C12Q1/003—Functionalisation
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
    • G01N27/26—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
    • G01N27/28—Electrolytic cell components
    • G01N27/30—Electrodes, e.g. test electrodes; Half-cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/34—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving hydrolase
    • C12Q1/37—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving hydrolase involving peptidase or proteinase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/48—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving transferase
    • C12Q1/485—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving transferase involving kinase
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10T—TECHNICAL SUBJECTS COVERED BY FORMER US CLASSIFICATION
    • Y10T29/00—Metal working
    • Y10T29/49—Method of mechanical manufacture
    • Y10T29/49002—Electrical device making
    • Y10T29/49117—Conductor or circuit manufacturing

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Zoology (AREA)
  • Engineering & Computer Science (AREA)
  • Immunology (AREA)
  • Physics & Mathematics (AREA)
  • General Health & Medical Sciences (AREA)
  • Molecular Biology (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biophysics (AREA)
  • Microbiology (AREA)
  • Genetics & Genomics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Electrochemistry (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
AU2008312352A 2007-10-17 2008-10-17 Electrochemical assay for the detection of enzymes Abandoned AU2008312352A1 (en)

Applications Claiming Priority (7)

Application Number Priority Date Filing Date Title
US98073307P 2007-10-17 2007-10-17
US60/980,733 2007-10-17
US8709408P 2008-08-07 2008-08-07
US8710208P 2008-08-07 2008-08-07
US61/087,094 2008-08-07
US61/087,102 2008-08-07
PCT/US2008/080363 WO2009052422A1 (en) 2007-10-17 2008-10-17 Electrochemical assay for the detection of enzymes

Publications (1)

Publication Number Publication Date
AU2008312352A1 true AU2008312352A1 (en) 2009-04-23

Family

ID=40567805

Family Applications (2)

Application Number Title Priority Date Filing Date
AU2008312352A Abandoned AU2008312352A1 (en) 2007-10-17 2008-10-17 Electrochemical assay for the detection of enzymes
AU2008311820A Abandoned AU2008311820A1 (en) 2007-10-17 2008-10-17 Novel chemistry used in biosensors

Family Applications After (1)

Application Number Title Priority Date Filing Date
AU2008311820A Abandoned AU2008311820A1 (en) 2007-10-17 2008-10-17 Novel chemistry used in biosensors

Country Status (6)

Country Link
US (5) US20100003710A1 (enExample)
EP (2) EP2212342A4 (enExample)
JP (2) JP2011502245A (enExample)
AU (2) AU2008312352A1 (enExample)
CA (2) CA2702969A1 (enExample)
WO (2) WO2009052422A1 (enExample)

Families Citing this family (33)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US8951400B2 (en) 2007-10-17 2015-02-10 Ohmx Corporation Chemistry used in biosensors
EP2278636A1 (en) * 2009-07-21 2011-01-26 Sony Corporation Uses of dithiocarbamate compounds
JP5352742B2 (ja) * 2009-08-07 2013-11-27 オームクス コーポレーション 酵素による酸化還元変化化学的脱離（ｅ−ｔｒａｃｅ）免疫学的検定法
WO2011062738A1 (en) * 2009-11-23 2011-05-26 Mikhail Briman Controlled electrochemical activation of carbon-based electrodes
US9603940B2 (en) 2010-04-06 2017-03-28 Ihi Corporation Metal salen complex derivative and process for production thereof
JP2013528379A (ja) 2010-05-21 2013-07-11 オームクス コーポレーション Ｐｓａ酵素活性アッセイによる癌の検出
WO2012100078A1 (en) * 2011-01-19 2012-07-26 Ohmx Corporation Enzyme triggered redox altering chemical elimination (e-trace) immmunoassay
US9638663B2 (en) 2011-07-25 2017-05-02 Proxim Diagnostics Corporation Cartridge for diagnostic testing
US10248293B2 (en) * 2011-09-30 2019-04-02 Nokia Technologies Oy Method, apparatus, computer program and user interface
FR2980989A1 (fr) * 2011-10-06 2013-04-12 Commissariat Energie Atomique Procede de fonctionnalisation d'un substrat solide autre qu'un substrat en or par des composes chimiques specifiques
JP6203183B2 (ja) 2011-10-17 2017-09-27 オームクス コーポレイション 酵素誘発レドックス変化化学的脱離（ｅ−ｔｒａｃｅ）イムノアッセイを使用する、ヘモグロビンａ１ｃのパーセンテージの単回直接検出
WO2013067349A1 (en) * 2011-11-04 2013-05-10 Ohmx Corporation Novel chemistry used in biosensors
WO2013080182A1 (en) * 2011-12-02 2013-06-06 Koninklijke Philips Electronics N.V. Method and device for detecting ph value of a solution
EP2802669A1 (en) 2012-01-09 2014-11-19 Ohmx Corporation Enzyme cascade methods for e-trace assay signal amplification
DE102012201843A1 (de) * 2012-02-08 2013-08-08 Siemens Aktiengesellschaft Anordnung und Verfahren zur elektrischen Detektion von flüssigen Proben mit Lateral Flow Assays
EP2877592B1 (en) 2012-07-27 2016-09-21 Ohmx Corporation Electronic measurements of monolayers following homogeneous reactions of their components
WO2014018899A1 (en) * 2012-07-27 2014-01-30 Ohmx Corporation Electric measurement of monolayers following pro-cleave detection of presence and activity of enzymes and other target analytes
GB2514825B (en) * 2013-06-06 2017-11-08 Bangor Univ Sensors
WO2015042493A2 (en) 2013-09-20 2015-03-26 Ohmx Corporation Psa enzymatic activity: a new biomarker for assessing prostate cancer aggressiveness
US9880121B2 (en) 2014-04-29 2018-01-30 Ohmx Corporation Bioelectronic binding assay using peak profiling
US20160041118A1 (en) * 2014-08-11 2016-02-11 Ohmx Corporation Enzyme triggered redox altering chemical elimination (e-trace) assay with multiplexing capabilities
WO2016061590A1 (en) 2014-10-17 2016-04-21 Wardell Mark R Method for detecting proteases and active infection in biological fluids and tissues
US9373561B1 (en) 2014-12-18 2016-06-21 International Business Machines Corporation Integrated circuit barrierless microfluidic channel
US20160178562A1 (en) * 2014-12-19 2016-06-23 Ohmx Corporation Competitive enzymatic assay
AU2016215455A1 (en) * 2015-02-02 2017-08-17 Ontera Inc. Labile linkers for biomarker detection
US20170030907A1 (en) * 2015-06-26 2017-02-02 Uti Limited Partnership Tlr-4 based electrochemical biosensor
CN105259349B (zh) * 2015-11-03 2017-11-14 青岛农业大学 一种免固定生物传感电极的制备及其在免标记均相光致电化学农残检测与癌症诊断中的应用
US11793893B2 (en) 2016-10-03 2023-10-24 The Penn State Research Foundation Compounds comprising conductive oligomers, materials formed therefrom, and methods of making and using same
BR112020006513A2 (pt) * 2017-11-08 2020-09-29 Mölnlycke Health Care Ab biossensor e sistema de curativo para lesão associado
CN108760845B (zh) * 2018-05-21 2020-06-09 浙江工业大学 一种改性pb修饰电极及其制备方法
US10908092B2 (en) 2018-10-11 2021-02-02 Baker Hughes, A Ge Company, Llc Process for making cyano functionalized gold nanoparticles
CN111385495B (zh) * 2018-12-29 2022-02-11 天津大学青岛海洋技术研究院 一种用于补偿tof传感器中电荷转移不完全的方法
KR102750082B1 (ko) * 2022-05-12 2025-01-03 서울대학교산학협력단 다중 복합 재료 및 상기 다중 복합 재료를 포함하는 금속 센서 및 상기 다중 복합 재료의 제조 방법

Family Cites Families (40)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0018002B1 (de) 1979-04-24 1983-02-09 Marcel Jozefonvicz Neues Bestimmungsverfahren für Proteasen und Antiproteasen
FR2673183B1 (fr) * 1991-02-21 1996-09-27 Asulab Sa Complexes mono, bis ou tris (2,2'-bipyridine substituee) d'un metal choisi parmi le fer, le ruthenium, l'osmium ou le vanadium et leurs procedes de preparation .
DE4430023A1 (de) * 1994-08-24 1996-02-29 Boehringer Mannheim Gmbh Elektrochemischer Sensor
WO1997021431A1 (en) 1995-12-12 1997-06-19 California Institute Of Technology Cobalt schiff base compounds
US5804400A (en) * 1996-02-05 1998-09-08 Igen International, Inc. Electrochemiluminescent assay
US6348319B1 (en) 1996-02-08 2002-02-19 Australian Membrane And Biotechnology Research Institute Enzyme detection biosensors
US6750016B2 (en) 1996-07-29 2004-06-15 Nanosphere, Inc. Nanoparticles having oligonucleotides attached thereto and uses therefor
US7045285B1 (en) * 1996-11-05 2006-05-16 Clinical Micro Sensors, Inc. Electronic transfer moieties attached to peptide nucleic acids
AU744503B2 (en) * 1997-06-12 2002-02-28 Clinical Micro Sensors, Inc. Electronic methods for the detection of analytes
US6013459A (en) 1997-06-12 2000-01-11 Clinical Micro Sensors, Inc. Detection of analytes using reorganization energy
US6134461A (en) 1998-03-04 2000-10-17 E. Heller & Company Electrochemical analyte
RU2161653C2 (ru) 1998-08-24 2001-01-10 ФАРМАКОВСКИЙ Дмитрий Александрович Способ количественного электрохимического анализа биомолекул
AU1241000A (en) 1998-10-27 2000-05-15 Clinical Micro Sensors, Inc. Detection of target analytes using particles and electrodes
US6432723B1 (en) * 1999-01-22 2002-08-13 Clinical Micro Sensors, Inc. Biosensors utilizing ligand induced conformation changes
CA2363827A1 (en) 1999-02-23 2000-08-31 Pentapharm Ag Oligopeptide derivatives for the electrochemical measurement of protease activity
US7234414B2 (en) 1999-11-18 2007-06-26 Sensortec Limited Mastitis detection
GB0002201D0 (en) 2000-01-31 2000-03-22 Imperial College Process
US6753143B2 (en) * 2000-05-01 2004-06-22 Clinical Micro Sensors, Inc. Target analyte detection using asymmetrical self-assembled monolayers
DE10024314A1 (de) 2000-05-17 2001-11-22 Basf Ag Verfahren, umfassend die indirekte elektrochemische Regeneration von NAD(P)H
US6676816B2 (en) * 2001-05-11 2004-01-13 Therasense, Inc. Transition metal complexes with (pyridyl)imidazole ligands and sensors using said complexes
US6932894B2 (en) * 2001-05-15 2005-08-23 Therasense, Inc. Biosensor membranes composed of polymers containing heterocyclic nitrogens
US8404096B2 (en) 2001-08-24 2013-03-26 Sensortec Limited Methods for producing highly sensitive potentiometric sensors
JP2005535120A (ja) * 2002-08-06 2005-11-17 アベシア・リミテッド 有機電子デバイス
US7214528B1 (en) 2002-12-31 2007-05-08 Oregon Health & Sciences University Device for direct electrical detection of molecules and molecule-molecule interactions
WO2004079848A2 (en) * 2003-03-03 2004-09-16 Yissum Research Development Company Of The Hebrew University Of Jerusalem Novel electrode with switchable and tunable power output and fuel cell using such electrode
JP4614630B2 (ja) * 2003-03-31 2011-01-19 有限会社筑波物質情報研究所 機能性高分子化合物およびそれを用いたバイオセンサ
WO2005024051A1 (en) 2003-06-23 2005-03-17 Vanderbilt University Cell-based assay for identifying peptidase inhibitors
AU2003903504A0 (en) 2003-07-08 2003-07-24 Johnson, Daniel Improvements in sensor chips
GB0316075D0 (en) 2003-07-09 2003-08-13 Molecular Sensing Plc Protease detection assay
WO2006093505A2 (en) 2004-05-10 2006-09-08 Northwestern University Compositions and methods for analyte detection
US7511142B2 (en) 2004-07-28 2009-03-31 Agency For Science, Technology And Research Mediator-modified redox biomolecules for use in electrochemical determination of analyte
ES2334671T3 (es) 2004-08-13 2010-03-15 Egomedical Technologies Ag Sistema de ensayo de analitos para determinar la concentracion de un analito en un fluido fisiologico o acuoso.
GB2420180A (en) 2004-11-11 2006-05-17 Sensor Tech Ltd Method of electrochemical analysis of an analyte
DE102005019302A1 (de) * 2005-04-26 2006-11-16 Toxogen Gmbh Carrier zum Targeting von Nervenzellen
US7728094B2 (en) * 2005-05-03 2010-06-01 Imec Selfassembled grafted polymeric layer for use in biosensor technology
JP2007000082A (ja) * 2005-06-23 2007-01-11 Sysmex Corp キナーゼ活性測定方法及び試薬
JP4858143B2 (ja) * 2006-02-22 2012-01-18 セイコーエプソン株式会社 膜形成方法、膜付基板、センサおよび液状組成物
WO2008045799A2 (en) 2006-10-06 2008-04-17 Nanomix, Inc. Electrochemical nanosensors for biomolecule detection
WO2010142037A1 (en) 2009-06-08 2010-12-16 The University Of Western Ontario An electrochemical method and apparatus of identifying the presence of a target
WO2011041586A1 (en) 2009-09-30 2011-04-07 Virogenomics, Inc. Electromagnetic detection of analytes

Also Published As

Publication number Publication date
US8802390B2 (en) 2014-08-12
JP2011502245A (ja) 2011-01-20
EP2210268A4 (en) 2012-02-15
WO2009052436A1 (en) 2009-04-23
EP2210268A1 (en) 2010-07-28
US20090253149A1 (en) 2009-10-08
CA2702969A1 (en) 2009-04-23
EP2212342A4 (en) 2010-11-17
JP2011501161A (ja) 2011-01-06
US20100003710A1 (en) 2010-01-07
US20120156709A1 (en) 2012-06-21
US8734631B2 (en) 2014-05-27
WO2009052422A1 (en) 2009-04-23
US20150198552A9 (en) 2015-07-16
AU2008311820A1 (en) 2009-04-23
US20140342383A1 (en) 2014-11-20
CA2702977A1 (en) 2009-04-23
US20140311922A1 (en) 2014-10-23
EP2212342A1 (en) 2010-08-04

Similar Documents

Publication Publication Date Title
US8802390B2 (en) Electrochemical assay for the detection of enzymes
JP2011502245A5 (enExample)
US9416390B2 (en) Electric measurement of monolayers following pro-cleave detection of presence and activity of enzymes and other target analytes
US9340567B2 (en) Chemistry used in biosensors
Hernick et al. Zinc hydrolases: the mechanisms of zinc-dependent deacetylases
Dzyadevych et al. Conductometric biosensors
JP2011501161A5 (enExample)
US8951400B2 (en) Chemistry used in biosensors
Evtugyn Biosensors: essentials
Saiapina et al. Development and optimization of a novel conductometric bi-enzyme biosensor for L-arginine determination
Cao et al. A simple and general approach to assay protease activity with electrochemical technique
CA2880101A1 (en) Electronic measurements of monolayers following homogeneous reactions of their components
Dou et al. A screen-printed, amperometric biosensor for the determination of organophosphorus pesticides in water samples
CA2860739A1 (en) Enzyme cascade methods for e-trace assay signal amplification
Hiratake Enzyme inhibitors as chemical tools to study enzyme catalysis: rational design, synthesis, and applications
Reid et al. Role of Ser216 in the mechanism of action of membrane-bound lytic transglycosylase B: further evidence for substrate-assisted catalysis
Wu et al. A novel electrochemical biosensor for highly selective detection of protease biomarker from Bacillus licheniformis with D-amino acid containing peptide
Jung et al. High-throughput investigation of transglutaminase 2 kinase regulation using a novel cysteine-modified peptide array
BILITEWSKI 3. BIOCHEMICAL PRINCIPLES
Meneghello A modular approach for a controlled immobilization of enzymes
Hüttl et al. Enzymatic kinetic determinations

Legal Events

Date Code Title Description
MK4 Application lapsed section 142(2)(d) - no continuation fee paid for the application