AU2008286254B2 - Method for synthesizing a cDNA in a sample in an enzymatic reaction - Google Patents

Method for synthesizing a cDNA in a sample in an enzymatic reaction Download PDF

Info

Publication number
AU2008286254B2
AU2008286254B2 AU2008286254A AU2008286254A AU2008286254B2 AU 2008286254 B2 AU2008286254 B2 AU 2008286254B2 AU 2008286254 A AU2008286254 A AU 2008286254A AU 2008286254 A AU2008286254 A AU 2008286254A AU 2008286254 B2 AU2008286254 B2 AU 2008286254B2
Authority
AU
Australia
Prior art keywords
reaction
poly
enzyme
primer
pcr
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Active
Application number
AU2008286254A
Other languages
English (en)
Other versions
AU2008286254A1 (en
Inventor
Holger Engel
Christian Korfhage
Martin Kreutz
Dirk Loffert
Subrahmanyam Yerramilli
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Qiagen GmbH
Original Assignee
Qiagen GmbH
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from PCT/EP2007/058369 external-priority patent/WO2008020008A1/de
Application filed by Qiagen GmbH filed Critical Qiagen GmbH
Publication of AU2008286254A1 publication Critical patent/AU2008286254A1/en
Application granted granted Critical
Publication of AU2008286254B2 publication Critical patent/AU2008286254B2/en
Active legal-status Critical Current
Anticipated expiration legal-status Critical

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6806—Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1096—Processes for the isolation, preparation or purification of DNA or RNA cDNA Synthesis; Subtracted cDNA library construction, e.g. RT, RT-PCR
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biomedical Technology (AREA)
  • Immunology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Plant Pathology (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
AU2008286254A 2007-08-13 2008-02-13 Method for synthesizing a cDNA in a sample in an enzymatic reaction Active AU2008286254B2 (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
AUPCT/EP2007/058369 2007-08-13
PCT/EP2007/058369 WO2008020008A1 (de) 2006-08-14 2007-08-13 VERFAHREN ZUR SYNTHESE EINER cDNA IN EINER PROBE IN EINER ENZYMATISCHEN REAKTION
PCT/EP2008/051763 WO2009021757A1 (de) 2007-08-13 2008-02-13 Verfahren zur synthese einer cdna in einer probe in einer enzymatischen reaktion

Publications (2)

Publication Number Publication Date
AU2008286254A1 AU2008286254A1 (en) 2009-02-19
AU2008286254B2 true AU2008286254B2 (en) 2014-01-30

Family

ID=39322429

Family Applications (1)

Application Number Title Priority Date Filing Date
AU2008286254A Active AU2008286254B2 (en) 2007-08-13 2008-02-13 Method for synthesizing a cDNA in a sample in an enzymatic reaction

Country Status (3)

Country Link
JP (1) JP5409631B2 (enrdf_load_stackoverflow)
AU (1) AU2008286254B2 (enrdf_load_stackoverflow)
WO (1) WO2009021757A1 (enrdf_load_stackoverflow)

Families Citing this family (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US9617606B2 (en) 2012-01-25 2017-04-11 Japan Science And Technology Agency Oligonucleotide for HIV detection, HIV detection kit, and HIV detection method
WO2016092062A1 (en) * 2014-12-11 2016-06-16 Qiagen Gmbh Method for improving the efficiency of cdna generation
WO2021168436A1 (en) * 2020-02-21 2021-08-26 Bioo Scientific Corporation Compositions and methods for generating massively parallel nucleic acid sequencing libraries
CN113151398A (zh) * 2021-05-07 2021-07-23 广州复能基因有限公司 外泌体中核酸分子的检测方法

Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20030113875A1 (en) * 1998-02-27 2003-06-19 Gemen Bob Van Method for the non-specific amplification of nucleic acid
WO2004044239A1 (en) * 2002-10-30 2004-05-27 Pamgene B.V. Improved methods for generating multiple rna copies
CN1763223A (zh) * 2004-10-19 2006-04-26 中国人民解放军军事医学科学院放射医学研究所 miRNA检测方法及试剂盒
WO2008092016A2 (en) * 2007-01-26 2008-07-31 Stratagene California Methods, compositions, and kits for detection of micro rna

Family Cites Families (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
ATE498022T1 (de) * 2003-12-23 2011-02-15 Genomic Health Inc Universelle vervielfältigung von fragmentierter rns
DE102006038113A1 (de) * 2006-08-14 2008-02-21 Qiagen Gmbh Verfahren zur Synthese einer cDNA in einer Probe in einer enzymatischen Reaktion und gleichzeitigen Bereitstellung eines ersten Enzyms mit Polyadenylierungsaktivität und eines zweiten Enzyms mit reverser Transkriptaseaktivität

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20030113875A1 (en) * 1998-02-27 2003-06-19 Gemen Bob Van Method for the non-specific amplification of nucleic acid
WO2004044239A1 (en) * 2002-10-30 2004-05-27 Pamgene B.V. Improved methods for generating multiple rna copies
CN1763223A (zh) * 2004-10-19 2006-04-26 中国人民解放军军事医学科学院放射医学研究所 miRNA检测方法及试剂盒
WO2008092016A2 (en) * 2007-01-26 2008-07-31 Stratagene California Methods, compositions, and kits for detection of micro rna

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
KO, J-H., and LEE, Y., Journal of Microbiological Methods, 2006, vol. 64, pp297-304 *

Also Published As

Publication number Publication date
WO2009021757A1 (de) 2009-02-19
AU2008286254A1 (en) 2009-02-19
JP2010535525A (ja) 2010-11-25
JP5409631B2 (ja) 2014-02-05

Similar Documents

Publication Publication Date Title
US20110124050A1 (en) Method for synthesizing a cdna in a sample in an enzymatic reaction
JP6768706B2 (ja) 液相におけるライゲーションアッセイ
EP3350732B1 (en) Method for preparing a next generation sequencing (ngs) library from a ribonucleic acid (rna) sample and kit for practicing the same
EP3058104B1 (en) Methods for adding adapters to nucleic acids and compositions for practicing the same
EP2660336B1 (en) Isothermal nucleic acid amplification
US8314220B2 (en) Methods compositions, and kits for detection of microRNA
EP1812599B1 (en) Methods and compositions for analysing ribonucleic acids
US7273730B2 (en) Nested PCR employing degradable primers
WO2008097957A2 (en) Detection of mature small rna molecules
WO2011085160A1 (en) Materials and methods for isothermal nucleic acid amplification
AU2008286254B2 (en) Method for synthesizing a cDNA in a sample in an enzymatic reaction
JP2015033390A (ja) Ｒｎａの正規化した定量化方法
EP3496727A1 (en) Constructs and methods for signal amplification
EP2191008B1 (de) Verfahren zur synthese einer cdna in einer probe in einer enzymatischen reaktion
CN105063190A (zh) 微小rna的固相芯片恒温检测方法
WO2023230335A1 (en) Method and kit for 3'-end modification of nucleic acids

Legal Events

Date Code Title Description
FGA Letters patent sealed or granted (standard patent)