AU2002346660A1 - Multiplex real-time quantitative pcr - Google Patents

Multiplex real-time quantitative pcr Download PDF

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Publication number
AU2002346660A1
AU2002346660A1 AU2002346660A AU2002346660A AU2002346660A1 AU 2002346660 A1 AU2002346660 A1 AU 2002346660A1 AU 2002346660 A AU2002346660 A AU 2002346660A AU 2002346660 A AU2002346660 A AU 2002346660A AU 2002346660 A1 AU2002346660 A1 AU 2002346660A1
Authority
AU
Australia
Prior art keywords
pair
pcr
seq
primer
target nucleic
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Abandoned
Application number
AU2002346660A
Other languages
English (en)
Inventor
Paul Coleman
Stavros Therianos
Min Zhu
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
University of Rochester
Original Assignee
University of Rochester
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by University of Rochester filed Critical University of Rochester
Publication of AU2002346660A1 publication Critical patent/AU2002346660A1/en
Priority to AU2009245826A priority Critical patent/AU2009245826A1/en
Abandoned legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/686—Polymerase chain reaction [PCR]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • C12Q1/6883—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for diseases caused by alterations of genetic material
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2600/00—Oligonucleotides characterized by their use
    • C12Q2600/158—Expression markers

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Analytical Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Physics & Mathematics (AREA)
  • Immunology (AREA)
  • Biotechnology (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Biophysics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Pathology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
AU2002346660A 2001-11-30 2002-12-02 Multiplex real-time quantitative pcr Abandoned AU2002346660A1 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
AU2009245826A AU2009245826A1 (en) 2001-11-30 2009-12-07 Mulitplex real-time quantitative PCR

Applications Claiming Priority (5)

Application Number Priority Date Filing Date Title
US33609501P 2001-11-30 2001-11-30
US60/336,095 2001-11-30
US39747502P 2002-07-19 2002-07-19
US60/397,475 2002-07-19
PCT/US2002/038806 WO2003048377A2 (en) 2001-11-30 2002-12-02 Multiplex real-time quantitative pcr

Related Child Applications (1)

Application Number Title Priority Date Filing Date
AU2009245826A Division AU2009245826A1 (en) 2001-11-30 2009-12-07 Mulitplex real-time quantitative PCR

Publications (1)

Publication Number Publication Date
AU2002346660A1 true AU2002346660A1 (en) 2003-06-17

Family

ID=26990035

Family Applications (2)

Application Number Title Priority Date Filing Date
AU2002346660A Abandoned AU2002346660A1 (en) 2001-11-30 2002-12-02 Multiplex real-time quantitative pcr
AU2009245826A Abandoned AU2009245826A1 (en) 2001-11-30 2009-12-07 Mulitplex real-time quantitative PCR

Family Applications After (1)

Application Number Title Priority Date Filing Date
AU2009245826A Abandoned AU2009245826A1 (en) 2001-11-30 2009-12-07 Mulitplex real-time quantitative PCR

Country Status (6)

Country Link
US (2) US20050089862A1 (https=)
EP (1) EP1461350A4 (https=)
JP (1) JP2005511055A (https=)
AU (2) AU2002346660A1 (https=)
CA (1) CA2468713A1 (https=)
WO (1) WO2003048377A2 (https=)

Families Citing this family (23)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
AU2003211947A1 (en) 2002-02-20 2003-09-09 Sysmex Corporation PRIMERS FOR NUCLEIC ACID AMPLIFICATION IN DETECTING HOUSEKEEPING GENE mRNA AND TEST METHOD USING THESE PRIMERS
CA2522062C (en) * 2003-04-11 2011-03-29 Dna Landmarks Inc. Methods for relative quantification of specific nucleic acid sequences
GB2401175A (en) * 2003-05-02 2004-11-03 Hong Kong Dna Chips Ltd Detection of SARS virus by PCR
US8062849B2 (en) * 2003-10-28 2011-11-22 The Johns Hopkins University Quantitative multiplex methylation-specific PCR
US20060057611A1 (en) * 2004-06-30 2006-03-16 Applera Corporation Log-linear amplification
JP4898210B2 (ja) * 2005-08-09 2012-03-14 株式会社ビー・エム・エル サポウイルスの検出方法
EP1777523A1 (en) 2005-10-19 2007-04-25 INSERM (Institut National de la Santé et de la Recherche Médicale) An in vitro method for the prognosis of progression of a cancer and of the outcome in a patient and means for performing said method
DE102006027675B4 (de) * 2006-06-14 2011-05-12 Siemens Ag Verfahren zur Bestimmung der Konzentration von Nukleinsäuren
US20080050724A1 (en) * 2006-08-24 2008-02-28 Microfluidic Systems, Inc. Method of detecting one or more limited copy targets
EP1961825A1 (en) 2007-02-26 2008-08-27 INSERM (Institut National de la Santé et de la Recherche Medicale) Method for predicting the occurrence of metastasis in breast cancer patients
CN101971033A (zh) 2007-06-04 2011-02-09 戴诺普雷克斯公司 用于结直肠癌的生物标志物组合
US20090253121A1 (en) * 2008-04-04 2009-10-08 Micah Halpern Method for amt-rflp dna fingerprinting
US8346485B2 (en) 2008-11-25 2013-01-01 Quest Diagnostics Investments Incorporated Methods and apparatuses for estimating initial target nucleic acid concentration in a sample by modeling background signal and cycle-dependent amplification efficiency of a polymerase chain reaction
FI20095544A0 (fi) * 2009-05-15 2009-05-15 Juha Kirveskari Menetelmä ja kitti antibioottiresistenttien bakteerien tunnistusta varten
AU2010276236B2 (en) 2009-07-21 2014-03-20 Gen-Probe Incorporated Methods and compositions for quantitative detection of nucleic acid sequences over an extended dynamic range
EP2721173A1 (en) 2011-06-14 2014-04-23 INSERM (Institut National de la Santé et de la Recherche Médicale) Methods for determining the expression level of a gene of interest including correction of rt-qpcr data for genomic dna-derived signals
EP2728015A1 (en) 2012-10-31 2014-05-07 INSERM (Institut National de la Santé et de la Recherche Médicale) Method for the Sezary's Syndrome diagnosis
CN106459993A (zh) 2014-03-31 2017-02-22 德彪药业国际股份公司 Fgfr融合体
WO2015153973A1 (en) * 2014-04-04 2015-10-08 Life Technologies Corporation Methods and systems for pcr quantitation
US10435747B2 (en) * 2014-08-19 2019-10-08 Arizona Board Of Regents On Behalf Of Arizona State University Radiation biodosimetry systems
EP3020827A1 (en) * 2014-11-13 2016-05-18 Carpegen GmbH Method for determining a property of a starting sample
JP7101397B2 (ja) * 2016-12-02 2022-07-15 学校法人近畿大学 遺伝子量の測定方法
JP6871598B2 (ja) * 2017-01-18 2021-05-12 国立大学法人広島大学 遺伝子の変異検出キット及び遺伝子の変異検出方法

Family Cites Families (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6180349B1 (en) * 1999-05-18 2001-01-30 The Regents Of The University Of California Quantitative PCR method to enumerate DNA copy number
US6605451B1 (en) * 2000-06-06 2003-08-12 Xtrana, Inc. Methods and devices for multiplexing amplification reactions
WO2002057496A2 (en) * 2001-01-18 2002-07-25 Socratech L.L.C. Gene expression profiling of endothelium in alzheimer's disease

Also Published As

Publication number Publication date
EP1461350A2 (en) 2004-09-29
WO2003048377A3 (en) 2004-05-06
CA2468713A1 (en) 2003-06-12
US20090081655A1 (en) 2009-03-26
JP2005511055A (ja) 2005-04-28
AU2009245826A1 (en) 2010-01-14
EP1461350A4 (en) 2006-07-26
WO2003048377A2 (en) 2003-06-12
US20050089862A1 (en) 2005-04-28

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DA3 Amendments made section 104

Free format text: THE NATURE OF THE AMENDMENT IS: ADD CO-INVENTOR THERIANOS, STAVROS

MK4 Application lapsed section 142(2)(d) - no continuation fee paid for the application