AU2002346660A1 - Multiplex real-time quantitative pcr - Google Patents

Multiplex real-time quantitative pcr Download PDF

Info

Publication number
AU2002346660A1
AU2002346660A1 AU2002346660A AU2002346660A AU2002346660A1 AU 2002346660 A1 AU2002346660 A1 AU 2002346660A1 AU 2002346660 A AU2002346660 A AU 2002346660A AU 2002346660 A AU2002346660 A AU 2002346660A AU 2002346660 A1 AU2002346660 A1 AU 2002346660A1
Authority
AU
Australia
Prior art keywords
pair
pcr
seq
primer
target nucleic
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Abandoned
Application number
AU2002346660A
Other languages
English (en)
Inventor
Paul Coleman
Stavros Therianos
Min Zhu
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
University of Rochester
Original Assignee
University of Rochester
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by University of Rochester filed Critical University of Rochester
Publication of AU2002346660A1 publication Critical patent/AU2002346660A1/en
Priority to AU2009245826A priority Critical patent/AU2009245826A1/en
Abandoned legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/686—Polymerase chain reaction [PCR]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • C12Q1/6883—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for diseases caused by alterations of genetic material
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2600/00—Oligonucleotides characterized by their use
    • C12Q2600/158—Expression markers

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Health & Medical Sciences (AREA)
  • Zoology (AREA)
  • Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Genetics & Genomics (AREA)
  • Analytical Chemistry (AREA)
  • Biophysics (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Immunology (AREA)
  • Biotechnology (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Pathology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
AU2002346660A 2001-11-30 2002-12-02 Multiplex real-time quantitative pcr Abandoned AU2002346660A1 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
AU2009245826A AU2009245826A1 (en) 2001-11-30 2009-12-07 Mulitplex real-time quantitative PCR

Applications Claiming Priority (5)

Application Number Priority Date Filing Date Title
US33609501P 2001-11-30 2001-11-30
US60/336,095 2001-11-30
US39747502P 2002-07-19 2002-07-19
US60/397,475 2002-07-19
PCT/US2002/038806 WO2003048377A2 (en) 2001-11-30 2002-12-02 Multiplex real-time quantitative pcr

Related Child Applications (1)

Application Number Title Priority Date Filing Date
AU2009245826A Division AU2009245826A1 (en) 2001-11-30 2009-12-07 Mulitplex real-time quantitative PCR

Publications (1)

Publication Number Publication Date
AU2002346660A1 true AU2002346660A1 (en) 2003-06-17

Family

ID=26990035

Family Applications (2)

Application Number Title Priority Date Filing Date
AU2002346660A Abandoned AU2002346660A1 (en) 2001-11-30 2002-12-02 Multiplex real-time quantitative pcr
AU2009245826A Abandoned AU2009245826A1 (en) 2001-11-30 2009-12-07 Mulitplex real-time quantitative PCR

Family Applications After (1)

Application Number Title Priority Date Filing Date
AU2009245826A Abandoned AU2009245826A1 (en) 2001-11-30 2009-12-07 Mulitplex real-time quantitative PCR

Country Status (6)

Country Link
US (2) US20050089862A1 (https=)
EP (1) EP1461350A4 (https=)
JP (1) JP2005511055A (https=)
AU (2) AU2002346660A1 (https=)
CA (1) CA2468713A1 (https=)
WO (1) WO2003048377A2 (https=)

Families Citing this family (23)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
AU2003211947A1 (en) 2002-02-20 2003-09-09 Sysmex Corporation PRIMERS FOR NUCLEIC ACID AMPLIFICATION IN DETECTING HOUSEKEEPING GENE mRNA AND TEST METHOD USING THESE PRIMERS
WO2004090160A1 (en) * 2003-04-11 2004-10-21 Dna Landmarks Inc. Methods for relative quantification of specific nucleic acid sequences
GB2401175A (en) * 2003-05-02 2004-11-03 Hong Kong Dna Chips Ltd Detection of SARS virus by PCR
WO2005042713A2 (en) 2003-10-28 2005-05-12 The Johns Hopkins University Quantitative multiplex methylation-specific pcr
US20060057611A1 (en) * 2004-06-30 2006-03-16 Applera Corporation Log-linear amplification
JP4898210B2 (ja) * 2005-08-09 2012-03-14 株式会社ビー・エム・エル サポウイルスの検出方法
EP1777523A1 (en) 2005-10-19 2007-04-25 INSERM (Institut National de la Santé et de la Recherche Médicale) An in vitro method for the prognosis of progression of a cancer and of the outcome in a patient and means for performing said method
DE102006027675B4 (de) 2006-06-14 2011-05-12 Siemens Ag Verfahren zur Bestimmung der Konzentration von Nukleinsäuren
US20080050724A1 (en) * 2006-08-24 2008-02-28 Microfluidic Systems, Inc. Method of detecting one or more limited copy targets
EP1961825A1 (en) 2007-02-26 2008-08-27 INSERM (Institut National de la Santé et de la Recherche Medicale) Method for predicting the occurrence of metastasis in breast cancer patients
AU2008300338A1 (en) 2007-06-04 2009-03-26 Diagnoplex Biomarker combinations for colorectal cancer
US20090253121A1 (en) * 2008-04-04 2009-10-08 Micah Halpern Method for amt-rflp dna fingerprinting
US8346485B2 (en) 2008-11-25 2013-01-01 Quest Diagnostics Investments Incorporated Methods and apparatuses for estimating initial target nucleic acid concentration in a sample by modeling background signal and cycle-dependent amplification efficiency of a polymerase chain reaction
FI20095544A0 (fi) * 2009-05-15 2009-05-15 Juha Kirveskari Menetelmä ja kitti antibioottiresistenttien bakteerien tunnistusta varten
CA2768391C (en) 2009-07-21 2016-09-06 Gen-Probe Incorporated Methods and compositions for quantitative detection of nucleic acid sequences over an extended dynamic range
WO2012171997A1 (en) 2011-06-14 2012-12-20 INSERM (Institut National de la Santé et de la Recherche Médicale) Methods for determining the expression level of a gene of interest including correction of rt-qpcr data for genomic dna-derived signals
EP2728015A1 (en) 2012-10-31 2014-05-07 INSERM (Institut National de la Santé et de la Recherche Médicale) Method for the Sezary's Syndrome diagnosis
SG11201607772WA (en) 2014-03-31 2016-10-28 Debiopharm Int Sa Fgfr fusions
WO2015153973A1 (en) * 2014-04-04 2015-10-08 Life Technologies Corporation Methods and systems for pcr quantitation
US10435747B2 (en) * 2014-08-19 2019-10-08 Arizona Board Of Regents On Behalf Of Arizona State University Radiation biodosimetry systems
EP3020827A1 (en) * 2014-11-13 2016-05-18 Carpegen GmbH Method for determining a property of a starting sample
JP7101397B2 (ja) * 2016-12-02 2022-07-15 学校法人近畿大学 遺伝子量の測定方法
JP6871598B2 (ja) * 2017-01-18 2021-05-12 国立大学法人広島大学 遺伝子の変異検出キット及び遺伝子の変異検出方法

Family Cites Families (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6180349B1 (en) * 1999-05-18 2001-01-30 The Regents Of The University Of California Quantitative PCR method to enumerate DNA copy number
US6605451B1 (en) * 2000-06-06 2003-08-12 Xtrana, Inc. Methods and devices for multiplexing amplification reactions
WO2002057496A2 (en) * 2001-01-18 2002-07-25 Socratech L.L.C. Gene expression profiling of endothelium in alzheimer's disease

Also Published As

Publication number Publication date
WO2003048377A3 (en) 2004-05-06
JP2005511055A (ja) 2005-04-28
EP1461350A4 (en) 2006-07-26
US20050089862A1 (en) 2005-04-28
CA2468713A1 (en) 2003-06-12
AU2009245826A1 (en) 2010-01-14
WO2003048377A2 (en) 2003-06-12
US20090081655A1 (en) 2009-03-26
EP1461350A2 (en) 2004-09-29

Similar Documents

Publication Publication Date Title
US20090081655A1 (en) Multiplex Real-time Quantitative PCR
EP3133167B1 (en) Methods relating to fusions of alk for diagnosing cancer
EP3455375B1 (en) Detection of met exon 14 deletions
CA2439074A1 (en) Methods and compositions for amplification of rna sequences
EP2663654A1 (en) Methods, compositions, and kits for detecting rare cells
WO2013119950A2 (en) Methods and compositions relating to fusions of ros1 for diagnosing and treating cancer
CN110418850A (zh) 鉴定和使用小rna预测因子的方法
Wang et al. High-throughput primer and probe design
Leat et al. Properties of novel and widely studied Y-STR loci in three South African populations
JP4891985B2 (ja) 肺炎球菌ｄｎａ検出用リアルタイムｐｃｒアッセイの開発および肺炎球菌疾患の診断
KR102438915B1 (ko) 표적 염기 배열을 검출하는 방법 프로브를 설계 및 제조하는 방법 및 키트
US20150232940A1 (en) Methods and compositions relating to diagnosing and treating receptor tyrosine kinase related cancers
EP3592860B1 (en) Rhd gene allele associated with a weak d phenotype and its uses
CN113444808B (zh) Loc114108859及其新用途
US20110045468A1 (en) Polynucleotides for the identification and quantification of group a streptococcus nucleic acids
KR101798874B1 (ko) 변이 검출용 프로브, 변이 검출 방법, 약효 판정 방법 및 변이 검출용 키트
WO2015168057A2 (en) Methods and compositions relating to fusions of alk for diagnosing and treating cancer
EP1581647A2 (en) Rapid direct sequence analysis of multi-exon genes
WO2013052663A1 (en) Methods and compositions for detecting a target dna in a mixed nucleic acid sample
Nam et al. Accurate quantification of human miRNA isoforms using the PROMER technology
Chowdhury et al. Case Studies—Development of Oligonucleotides
Jung et al. Novel multiplex and quantitative genetic analysis method by CE-based SSCP

Legal Events

Date Code Title Description
DA3 Amendments made section 104

Free format text: THE NATURE OF THE AMENDMENT IS: ADD CO-INVENTOR THERIANOS, STAVROS

MK4 Application lapsed section 142(2)(d) - no continuation fee paid for the application