AU2002225605A1 - Method for removing a universal linker from an oligonucleotide - Google Patents

Method for removing a universal linker from an oligonucleotide

Info

Publication number
AU2002225605A1
AU2002225605A1 AU2002225605A AU2560502A AU2002225605A1 AU 2002225605 A1 AU2002225605 A1 AU 2002225605A1 AU 2002225605 A AU2002225605 A AU 2002225605A AU 2560502 A AU2560502 A AU 2560502A AU 2002225605 A1 AU2002225605 A1 AU 2002225605A1
Authority
AU
Australia
Prior art keywords
linker
oligonucleotide
cleavage
solid support
universal
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Abandoned
Application number
AU2002225605A
Other languages
English (en)
Inventor
Gulilat Gebeyehu
Richard M Pires
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Life Technologies Corp
Original Assignee
Invitrogen Corp
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Invitrogen Corp filed Critical Invitrogen Corp
Publication of AU2002225605A1 publication Critical patent/AU2002225605A1/en
Abandoned legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
    • C07H21/00—Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids

Landscapes

  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Biochemistry (AREA)
  • Molecular Biology (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Saccharide Compounds (AREA)
AU2002225605A 2000-11-09 2001-11-08 Method for removing a universal linker from an oligonucleotide Abandoned AU2002225605A1 (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US24671300P 2000-11-09 2000-11-09
US60/, 2000-11-09
PCT/US2001/043013 WO2002038728A2 (en) 2000-11-09 2001-11-08 Method for removing a universal linker from an oligonucleotide

Publications (1)

Publication Number Publication Date
AU2002225605A1 true AU2002225605A1 (en) 2002-05-21

Family

ID=22931883

Family Applications (1)

Application Number Title Priority Date Filing Date
AU2002225605A Abandoned AU2002225605A1 (en) 2000-11-09 2001-11-08 Method for removing a universal linker from an oligonucleotide

Country Status (7)

Country Link
US (1) US20020143166A1 (ja)
EP (1) EP1337542A2 (ja)
JP (1) JP2004513629A (ja)
AU (1) AU2002225605A1 (ja)
CA (1) CA2428155A1 (ja)
NZ (1) NZ525683A (ja)
WO (1) WO2002038728A2 (ja)

Families Citing this family (12)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CA2375060A1 (en) 1999-05-24 2000-11-30 Invitrogen Corporation Method for deblocking of labeled oligonucleotides
US6803200B2 (en) 2000-12-12 2004-10-12 Invitrogen Corporation Compositions and methods for the release of nucleic acid molecules from solid matrices
WO2005097817A2 (en) 2004-04-05 2005-10-20 Alnylam Pharmaceuticals, Inc. Process and reagents for oligonucleotide synthesis and purification
JP4584986B2 (ja) * 2004-04-27 2010-11-24 アルニラム ファーマスーティカルズ インコーポレイテッド ２−アリールプロピル部分を含む１本鎖及び２本鎖オリゴヌクレオチド
WO2006088490A2 (en) * 2004-06-30 2006-08-24 Alnylam Pharmaceuticals, Inc. Oligonucleotides comprising a non-phosphate backbone linkage
WO2006093526A2 (en) 2004-07-21 2006-09-08 Alnylam Pharmaceuticals, Inc. Oligonucleotides comprising a modified or non-natural nucleobase
AU2005330637B2 (en) 2004-08-04 2012-09-20 Alnylam Pharmaceuticals, Inc. Oligonucleotides comprising a ligand tethered to a modified or non-natural nucleobase
US20110117660A1 (en) * 2009-11-16 2011-05-19 Brooks Douglas G Physical characterization of oligonucleotide conjugates
WO2014153188A2 (en) 2013-03-14 2014-09-25 Life Technologies Corporation High efficiency, small volume nucleic acid synthesis
CN107058059B (zh) 2011-09-26 2020-08-07 基因技术股份公司 高效的小体积核酸合成
EP3557262B1 (en) 2014-12-09 2022-08-10 Life Technologies Corporation High efficiency, small volume nucleic acid synthesis
CN110446718A (zh) * 2017-03-29 2019-11-12 罗氏创新中心哥本哈根有限公司 Unylinker快速裂解

Family Cites Families (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4458066A (en) * 1980-02-29 1984-07-03 University Patents, Inc. Process for preparing polynucleotides
US4415732A (en) * 1981-03-27 1983-11-15 University Patents, Inc. Phosphoramidite compounds and processes
US4757141A (en) * 1985-08-26 1988-07-12 Applied Biosystems, Incorporated Amino-derivatized phosphite and phosphate linking agents, phosphoramidite precursors, and useful conjugates thereof
US5514789A (en) * 1994-04-21 1996-05-07 Barrskogen, Inc. Recovery of oligonucleotides by gas phase cleavage

Also Published As

Publication number Publication date
US20020143166A1 (en) 2002-10-03
NZ525683A (en) 2004-10-29
CA2428155A1 (en) 2002-05-16
JP2004513629A (ja) 2004-05-13
WO2002038728A2 (en) 2002-05-16
EP1337542A2 (en) 2003-08-27
WO2002038728A3 (en) 2002-09-06

Similar Documents

Publication Publication Date Title
US5646260A (en) Oligonucleotides having modified internucleoside linkages
CA2017369C (en) Modified phosphoramidite process for the production or modified nucleic acids
KR101032008B1 (ko) 폴리뉴클레오티드 표지 시약
US5252723A (en) Method and reagent for sulfurization of organophosphorous compounds
WO1998008858A1 (en) Improved reagents and process for synthesis of oligonucleotides containing phosphorodithioate internucleoside linkages
US20020143166A1 (en) Method for removing a universal linker from an oligonucleotide
WO1999062922A1 (en) Activators for oligonucleotide synthesis
CA2375060A1 (en) Method for deblocking of labeled oligonucleotides
EP1149109B1 (en) Method for deprotecting oligonucleotides
EP0843684A2 (en) Universal solid supports and methods for their use
US6965041B1 (en) N-acylphosphoramidites and their use in oligonucleotide synthesis
US6605708B1 (en) Building blocks with carbamate internucleoside linkages and oligonucleotides derived therefrom
US5606049A (en) Method of preparing 2'-O-methyl cytidine monomers useful in oligomer synthesis
US4786724A (en) Nucleoside trichlorodimethylethyl phosphite esters
WO2000055170A1 (en) Methods for removing dimethoxytrityl groups from oligonucleotides
AU642673B2 (en) Method and reagent for sulfurization of organophosphorous compounds
JP4579350B2 (ja) 共有結合的に架橋されたオリゴヌクレオチド、その製造法、およびその製造法に用いられるシントン
CA3141195A1 (en) Process for the preparation of oligonucleotides using modified oxidation protocol.
WO2012047639A2 (en) Purification of synthetic oligonucleotides
WO2000049032A1 (en) Oligonucleotides having alkylphosphonate linkages and methods for their preparation
WO2021243443A1 (en) Method for the preparation of oligonucleotides
WO1996023808A1 (en) Improved methods for h-phosphonate synthesis of oligonucleotides

Legal Events

Date Code Title Description
MK4 Application lapsed section 142(2)(d) - no continuation fee paid for the application