AU2001274635A1 - Method for generating recombinant dna library using unidirectional single-stranded dna fragments - Google Patents

Method for generating recombinant dna library using unidirectional single-stranded dna fragments

Info

Publication number
AU2001274635A1
AU2001274635A1 AU2001274635A AU7463501A AU2001274635A1 AU 2001274635 A1 AU2001274635 A1 AU 2001274635A1 AU 2001274635 A AU2001274635 A AU 2001274635A AU 7463501 A AU7463501 A AU 7463501A AU 2001274635 A1 AU2001274635 A1 AU 2001274635A1
Authority
AU
Australia
Prior art keywords
generating recombinant
unidirectional single
recombinant dna
stranded dna
dna fragments
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Abandoned
Application number
AU2001274635A
Other languages
English (en)
Inventor
Yeong Joong Jeon
Kyung Hwa Jung
Ko Woon Lee
Si Hyoung Lee
Eun Jung Ryu
Yong Chul Shin
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Amicogen Inc
Original Assignee
Amicogen Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Amicogen Inc filed Critical Amicogen Inc
Publication of AU2001274635A1 publication Critical patent/AU2001274635A1/en
Abandoned legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/102—Mutagenizing nucleic acids
    • C12N15/1027—Mutagenizing nucleic acids by DNA shuffling, e.g. RSR, STEP, RPR
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA

Landscapes

  • Genetics & Genomics (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • General Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biomedical Technology (AREA)
  • Organic Chemistry (AREA)
  • Biotechnology (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Microbiology (AREA)
  • Plant Pathology (AREA)
  • Physics & Mathematics (AREA)
  • Molecular Biology (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Biophysics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
AU2001274635A 2000-11-10 2001-06-16 Method for generating recombinant dna library using unidirectional single-stranded dna fragments Abandoned AU2001274635A1 (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
KR20000066889 2000-11-10
KR0066889 2000-11-10
PCT/KR2001/001031 WO2002038757A1 (en) 2000-11-10 2001-06-16 Method for generating recombinant dna library using unidirectional single-stranded dna fragments

Publications (1)

Publication Number Publication Date
AU2001274635A1 true AU2001274635A1 (en) 2002-05-21

Family

ID=19698438

Family Applications (1)

Application Number Title Priority Date Filing Date
AU2001274635A Abandoned AU2001274635A1 (en) 2000-11-10 2001-06-16 Method for generating recombinant dna library using unidirectional single-stranded dna fragments

Country Status (5)

Country Link
US (1) US6955879B2 (ko)
JP (1) JP3777158B2 (ko)
KR (1) KR100446417B1 (ko)
AU (1) AU2001274635A1 (ko)
WO (1) WO2002038757A1 (ko)

Families Citing this family (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2003097834A2 (en) * 2002-05-17 2003-11-27 Alligator Bioscience Ab A method for in vitro molecular evolution of protein function
WO2004009814A1 (en) * 2002-07-23 2004-01-29 Nuevolution A/S Gene shuffling by template switching
AU2009322346B2 (en) 2008-12-03 2015-07-02 The Scripps Research Institute Stem cell cultures
US9410173B2 (en) 2012-10-24 2016-08-09 Clontech Laboratories, Inc. Template switch-based methods for producing a product nucleic acid
CN105658815B (zh) 2013-10-17 2020-12-29 塔卡拉生物美国有限公司 用于添加适配体至核酸的方法及用于实施所述方法的组合物
US9719136B2 (en) 2013-12-17 2017-08-01 Takara Bio Usa, Inc. Methods for adding adapters to nucleic acids and compositions for practicing the same
JP2023542064A (ja) * 2020-06-23 2023-10-05 ハイムバイオテック インコーポレイテッド 逐次ポリメラーゼ連鎖反応用組成物及びそれを用いた遺伝子増幅方法
CN112175954B (zh) * 2020-10-19 2022-09-09 复旦大学附属肿瘤医院 Iv类脱氧核酶突变体及其制备方法与应用

Family Cites Families (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4994368A (en) * 1987-07-23 1991-02-19 Syntex (U.S.A.) Inc. Amplification method for polynucleotide assays
US5605793A (en) * 1994-02-17 1997-02-25 Affymax Technologies N.V. Methods for in vitro recombination
US5965408A (en) * 1996-07-09 1999-10-12 Diversa Corporation Method of DNA reassembly by interrupting synthesis
US5962271A (en) * 1996-01-03 1999-10-05 Cloutech Laboratories, Inc. Methods and compositions for generating full-length cDNA having arbitrary nucleotide sequence at the 3'-end
US6153410A (en) * 1997-03-25 2000-11-28 California Institute Of Technology Recombination of polynucleotide sequences using random or defined primers
KR100430534B1 (ko) * 2001-05-23 2004-05-10 주식회사 마이크로아이디 단일가닥 디엔에이 분해효소를 이용하여 키메라 디엔에이라이브러리를 만드는 방법

Also Published As

Publication number Publication date
US6955879B2 (en) 2005-10-18
US20030152943A1 (en) 2003-08-14
JP2004513633A (ja) 2004-05-13
KR20020036665A (ko) 2002-05-16
JP3777158B2 (ja) 2006-05-24
KR100446417B1 (ko) 2004-09-04
WO2002038757A1 (en) 2002-05-16

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