AU2001251120A1 - Sigma binding region of rna polymerase and uses thereof - Google Patents

Sigma binding region of rna polymerase and uses thereof

Info

Publication number
AU2001251120A1
AU2001251120A1 AU2001251120A AU5112001A AU2001251120A1 AU 2001251120 A1 AU2001251120 A1 AU 2001251120A1 AU 2001251120 A AU2001251120 A AU 2001251120A AU 5112001 A AU5112001 A AU 5112001A AU 2001251120 A1 AU2001251120 A1 AU 2001251120A1
Authority
AU
Australia
Prior art keywords
subunit
rna polymerase
binding
protein
residues
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Abandoned
Application number
AU2001251120A
Other languages
English (en)
Inventor
Larry C. Anthony
Terrance M. Arthur
Veit Bergendahl
Richard R. Burgess
Bradley C. Pietz
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Wisconsin Alumni Research Foundation
Original Assignee
BRADLEY C PIETZ
LARRY C ANTHONY
RICHARD R BURGESS
TERRANCE M ARTHUR
Wisconsin Alumni Research Foundation
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by BRADLEY C PIETZ, LARRY C ANTHONY, RICHARD R BURGESS, TERRANCE M ARTHUR, Wisconsin Alumni Research Foundation filed Critical BRADLEY C PIETZ
Publication of AU2001251120A1 publication Critical patent/AU2001251120A1/en
Abandoned legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/48—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving transferase
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/573—Immunoassay; Biospecific binding assay; Materials therefor for enzymes or isoenzymes
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2333/00—Assays involving biological materials from specific organisms or of a specific nature
    • G01N2333/90—Enzymes; Proenzymes
    • G01N2333/91—Transferases (2.)
    • G01N2333/912—Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
    • G01N2333/91205—Phosphotransferases in general
    • G01N2333/91245—Nucleotidyltransferases (2.7.7)
    • G01N2333/9125—Nucleotidyltransferases (2.7.7) with a definite EC number (2.7.7.-)
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2500/00—Screening for compounds of potential therapeutic value
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2500/00—Screening for compounds of potential therapeutic value
    • G01N2500/20—Screening for compounds of potential therapeutic value cell-free systems

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Immunology (AREA)
  • Organic Chemistry (AREA)
  • Molecular Biology (AREA)
  • Urology & Nephrology (AREA)
  • Biotechnology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Microbiology (AREA)
  • Biomedical Technology (AREA)
  • Analytical Chemistry (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Biochemistry (AREA)
  • Physics & Mathematics (AREA)
  • Hematology (AREA)
  • General Health & Medical Sciences (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Physics & Mathematics (AREA)
  • Cell Biology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biophysics (AREA)
  • Food Science & Technology (AREA)
  • Medicinal Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • Pathology (AREA)
  • Peptides Or Proteins (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
AU2001251120A 2000-03-30 2001-03-30 Sigma binding region of rna polymerase and uses thereof Abandoned AU2001251120A1 (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US19311600P 2000-03-30 2000-03-30
US60193116 2000-03-30
PCT/US2001/010170 WO2001075143A2 (en) 2000-03-30 2001-03-30 Sigma binding region of rna polymerase and uses thereof

Publications (1)

Publication Number Publication Date
AU2001251120A1 true AU2001251120A1 (en) 2001-10-15

Family

ID=22712336

Family Applications (1)

Application Number Title Priority Date Filing Date
AU2001251120A Abandoned AU2001251120A1 (en) 2000-03-30 2001-03-30 Sigma binding region of rna polymerase and uses thereof

Country Status (6)

Country Link
US (2) US6613531B2 (de)
EP (1) EP1278886A2 (de)
JP (1) JP2004500122A (de)
AU (1) AU2001251120A1 (de)
CA (1) CA2404498A1 (de)
WO (1) WO2001075143A2 (de)

Families Citing this family (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
AU2002307503A1 (en) * 2001-04-25 2002-11-05 Irina E. Artsimovitch Methods to identify agents that bind the flap-tip helix of rna polymerase
AU2003299378A1 (en) * 2002-10-11 2004-05-04 Board Of Regents, The University Of Texas System Method and compounds for inhibiting hec1 activity for the treatment of proliferative diseases
US20040157314A1 (en) * 2003-02-07 2004-08-12 Phagetech Inc. Inhibitors of Staphylococcus aureus primary sigma factor and uses thereof
CN1886661B (zh) * 2003-10-01 2010-06-02 沃拉克有限公司 改进的均匀时间分辨能量转移测定
FR2890174B1 (fr) * 2005-08-30 2009-04-24 Cis Bio Internat Sa Procede pour la mise en evidence d'un processus biologique par mesure d'un fret

Family Cites Families (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB9312947D0 (en) 1993-06-23 1993-08-04 Chasteau Ronald C M Saw and router tool carrier and table
IE960132A1 (en) * 1995-03-13 1996-09-18 Astra Ab New DNA molecules
CA2180077A1 (en) 1995-07-12 1997-01-13 Astra Ab RNA POLYMERASE INHIBITORS
JP2000508525A (ja) * 1996-03-27 2000-07-11 ザ・ジョンズ・ホプキンス・ユニバーシティー マイコバクテリウム・チュバキュローシスの定常期ストレス応答σ因子およびその制御
US5824546A (en) * 1996-03-27 1998-10-20 The Johns Hopkins University Regulation of a sigma factor from Mycobacterium tuberculosis
CA2278314A1 (en) * 1997-01-16 1998-07-23 Scriptgen Pharmaceuticals, Inc. Nucleic acid encoding m. tuberculosis algu protein
DE69938103T2 (de) 1998-06-09 2009-01-29 Astrazeneca Ab Verfahren zum identifizieren von liganden der rna-polymerase sigma 70 untereinheit

Also Published As

Publication number Publication date
WO2001075143A3 (en) 2002-08-22
US20020127688A1 (en) 2002-09-12
US6613531B2 (en) 2003-09-02
CA2404498A1 (en) 2001-10-11
JP2004500122A (ja) 2004-01-08
EP1278886A2 (de) 2003-01-29
US20030104423A1 (en) 2003-06-05
WO2001075143A2 (en) 2001-10-11
US6905866B2 (en) 2005-06-14

Similar Documents

Publication Publication Date Title
US7060506B2 (en) Compositions and methods for monitoring the modification of modification dependent binding partner polypeptides
US6972198B2 (en) Methods and compositions using protein binding partners
WO2008112008A9 (en) Sensor proteins and assay methods
EP2900830B1 (de) Umschaltbare reporterenzyme zum homogenen nachweis von antikörpern
US20120107836A1 (en) Development of fluorescently p-loop labelled kinases for screening of inhibitors
JP2011528908A (ja) ユビキチンリガーゼのモジュレーターの特定法
Lee et al. Structure and dynamics of the epidermal growth factor receptor C‐terminal phosphorylation domain
US6905866B2 (en) Sigma binding region of RNA polymerase and uses thereof
US8323919B2 (en) Assay methods for identifying glycogen synthase kinase 3 modulators
US10794915B2 (en) Genetically encoded sensors for imaging proteins and their complexes
AU2011247141A1 (en) Ubiquitination assay
WO2011135301A1 (en) Ubiquitination assay
Bergendahl et al. On-column tris (2-carboxyethyl) phosphine reduction and IC5-maleimide labeling during purification of a RpoC fragment on a nickel–nitrilotriacetic acid column
JP5665262B2 (ja) Ｂｃｒ−ａｂｌチロシンキナーゼ活性測定用試薬
WO2011131934A1 (en) Ubiquitination assay
Glaser et al. LRET-based HTS of a small-compound library for inhibitors of bacterial RNA polymerase
JP2004053416A (ja) Ｃ末端標識タンパク質を用いるタンパク質−分子間相互作用の解析方法
WO2000050902A2 (en) High throughput assay based on the use of a polypeptide binding pair
Bergendahl et al. Studying sigma–core interactions in Escherichia coli RNA polymerase by electrophoretic shift assays and luminescence resonance energy transfer
Yamabhai BAP-fusion: A versatile molecular probe for biotechnology research
EP1412758A2 (de) Protein-arrays, verfahren zu ihrer herstellung sowie verfahren zum nachweis intermolekularer wechselwirkungen
EP1155326A1 (de) Proteinassay
GB2375538A (en) Polypeptide constructs for FRET analysis
Hu Protein-protein and protein-DNA interactions of the b/HLH/z transcription factors Myc/Max/Mad

Legal Events

Date Code Title Description
MK5 Application lapsed section 142(2)(e) - patent request and compl. specification not accepted