AU2001236726A1 - Protein separation and display - Google Patents

Protein separation and display

Info

Publication number
AU2001236726A1
AU2001236726A1 AU2001236726A AU3672601A AU2001236726A1 AU 2001236726 A1 AU2001236726 A1 AU 2001236726A1 AU 2001236726 A AU2001236726 A AU 2001236726A AU 3672601 A AU3672601 A AU 3672601A AU 2001236726 A1 AU2001236726 A1 AU 2001236726A1
Authority
AU
Australia
Prior art keywords
display
protein separation
protein
separation
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Abandoned
Application number
AU2001236726A
Other languages
English (en)
Inventor
Timothy J Barder
Bathsheba E. Chong
Thomas Horvath
Maureen T. Kachman
David M. Lubman
Robert North
Stephen J. Parus
Daniel B. Wall
Fang Yan
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
University of Michigan
Original Assignee
University of Michigan
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by University of Michigan filed Critical University of Michigan
Publication of AU2001236726A1 publication Critical patent/AU2001236726A1/en
Abandoned legal-status Critical Current

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
    • G01N33/6803—General methods of protein analysis not limited to specific proteins or families of proteins
    • G01N33/6842—Proteomic analysis of subsets of protein mixtures with reduced complexity, e.g. membrane proteins, phosphoproteins, organelle proteins
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/14—Extraction; Separation; Purification
    • C07K1/36—Extraction; Separation; Purification by a combination of two or more processes of different types
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
    • G01N30/02—Column chromatography
    • G01N30/26—Conditioning of the fluid carrier; Flow patterns
    • G01N30/38—Flow patterns
    • G01N30/46—Flow patterns using more than one column
    • G01N30/461—Flow patterns using more than one column with serial coupling of separation columns
    • G01N30/463—Flow patterns using more than one column with serial coupling of separation columns for multidimensional chromatography
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
    • G01N33/6803—General methods of protein analysis not limited to specific proteins or families of proteins
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
    • G01N30/02—Column chromatography
    • G01N2030/022—Column chromatography characterised by the kind of separation mechanism
    • G01N2030/027—Liquid chromatography
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
    • G01N30/02—Column chromatography
    • G01N30/50—Conditioning of the sorbent material or stationary liquid
    • G01N30/52—Physical parameters
    • G01N2030/524—Physical parameters structural properties
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
    • G01N30/02—Column chromatography
    • G01N30/88—Integrated analysis systems specially adapted therefor, not covered by a single one of the groups G01N30/04 - G01N30/86
    • G01N2030/8804—Integrated analysis systems specially adapted therefor, not covered by a single one of the groups G01N30/04 - G01N30/86 automated systems
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
    • G01N30/02—Column chromatography
    • G01N30/88—Integrated analysis systems specially adapted therefor, not covered by a single one of the groups G01N30/04 - G01N30/86
    • G01N2030/8809—Integrated analysis systems specially adapted therefor, not covered by a single one of the groups G01N30/04 - G01N30/86 analysis specially adapted for the sample
    • G01N2030/8813—Integrated analysis systems specially adapted therefor, not covered by a single one of the groups G01N30/04 - G01N30/86 analysis specially adapted for the sample biological materials
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
    • G01N30/02—Column chromatography
    • G01N30/88—Integrated analysis systems specially adapted therefor, not covered by a single one of the groups G01N30/04 - G01N30/86
    • G01N2030/8809—Integrated analysis systems specially adapted therefor, not covered by a single one of the groups G01N30/04 - G01N30/86 analysis specially adapted for the sample
    • G01N2030/8813—Integrated analysis systems specially adapted therefor, not covered by a single one of the groups G01N30/04 - G01N30/86 analysis specially adapted for the sample biological materials
    • G01N2030/8831—Integrated analysis systems specially adapted therefor, not covered by a single one of the groups G01N30/04 - G01N30/86 analysis specially adapted for the sample biological materials involving peptides or proteins
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
    • G01N27/26—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
    • G01N27/416—Systems
    • G01N27/447—Systems using electrophoresis
    • G01N27/44756—Apparatus specially adapted therefor
    • G01N27/44795—Isoelectric focusing
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
    • G01N30/02—Column chromatography
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
    • G01N30/02—Column chromatography
    • G01N30/26—Conditioning of the fluid carrier; Flow patterns
    • G01N30/38—Flow patterns
    • G01N30/46—Flow patterns using more than one column
    • G01N30/461—Flow patterns using more than one column with serial coupling of separation columns
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
    • G01N30/02—Column chromatography
    • G01N30/62—Detectors specially adapted therefor
    • G01N30/72—Mass spectrometers
    • G01N30/7233—Mass spectrometers interfaced to liquid or supercritical fluid chromatograph
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
    • G01N30/02—Column chromatography
    • G01N30/80—Fraction collectors
    • G01N30/82—Automatic means therefor
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
    • G01N30/02—Column chromatography
    • G01N30/86—Signal analysis
    • G01N30/8651—Recording, data aquisition, archiving and storage
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10T—TECHNICAL SUBJECTS COVERED BY FORMER US CLASSIFICATION
    • Y10T436/00—Chemistry: analytical and immunological testing
    • Y10T436/24—Nuclear magnetic resonance, electron spin resonance or other spin effects or mass spectrometry
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10T—TECHNICAL SUBJECTS COVERED BY FORMER US CLASSIFICATION
    • Y10T436/00—Chemistry: analytical and immunological testing
    • Y10T436/25—Chemistry: analytical and immunological testing including sample preparation
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10T—TECHNICAL SUBJECTS COVERED BY FORMER US CLASSIFICATION
    • Y10T436/00—Chemistry: analytical and immunological testing
    • Y10T436/25—Chemistry: analytical and immunological testing including sample preparation
    • Y10T436/25375—Liberation or purification of sample or separation of material from a sample [e.g., filtering, centrifuging, etc.]
    • Y10T436/255—Liberation or purification of sample or separation of material from a sample [e.g., filtering, centrifuging, etc.] including use of a solid sorbent, semipermeable membrane, or liquid extraction

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Physics & Mathematics (AREA)
  • Immunology (AREA)
  • Urology & Nephrology (AREA)
  • Biochemistry (AREA)
  • Analytical Chemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Hematology (AREA)
  • Biomedical Technology (AREA)
  • Biophysics (AREA)
  • Medicinal Chemistry (AREA)
  • Pathology (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • General Physics & Mathematics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Cell Biology (AREA)
  • Microbiology (AREA)
  • Food Science & Technology (AREA)
  • Biotechnology (AREA)
  • Organic Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Other Investigation Or Analysis Of Materials By Electrical Means (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
AU2001236726A 2000-02-08 2001-02-07 Protein separation and display Abandoned AU2001236726A1 (en)

Applications Claiming Priority (9)

Application Number Priority Date Filing Date Title
US18091100P 2000-02-08 2000-02-08
US60/180,911 2000-02-08
US23932600P 2000-10-10 2000-10-10
US23932500P 2000-10-10 2000-10-10
US60/239,325 2000-10-10
US60/239,326 2000-10-10
US25944801P 2001-01-03 2001-01-03
US60/259,448 2001-01-03
PCT/US2001/003917 WO2001058925A2 (fr) 2000-02-08 2001-02-07 Separation et affichage de proteines

Publications (1)

Publication Number Publication Date
AU2001236726A1 true AU2001236726A1 (en) 2001-08-20

Family

ID=27497425

Family Applications (1)

Application Number Title Priority Date Filing Date
AU2001236726A Abandoned AU2001236726A1 (en) 2000-02-08 2001-02-07 Protein separation and display

Country Status (7)

Country Link
US (1) US20020098595A1 (fr)
EP (1) EP1358202A2 (fr)
JP (1) JP2004500567A (fr)
AU (1) AU2001236726A1 (fr)
CA (1) CA2400484A1 (fr)
MX (1) MXPA02007664A (fr)
WO (1) WO2001058925A2 (fr)

Families Citing this family (31)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2003534533A (ja) 2000-02-08 2003-11-18 ザ リージェンツ オブ ザ ユニバーシティ オブ ミシガン タンパク質のディファレンシャルディスプレイのマッピング
US20030064527A1 (en) * 2001-02-07 2003-04-03 The Regents Of The University Of Michigan Proteomic differential display
US20050230315A1 (en) * 2003-01-13 2005-10-20 Regents Of The University Of Michigan Protein microarray system
US6931325B2 (en) 2001-02-07 2005-08-16 Regents Of The University Of Michigan Three dimensional protein mapping
EP1456667B2 (fr) * 2001-12-08 2010-01-20 Micromass UK Limited Procede de spectrometrie de masse
EP1391723B1 (fr) * 2002-08-16 2006-06-21 Agilent Technologies Inc. a Delaware Corporation Méthode et appareil pour analyse d'un mélange des échantillons substances
GB2409038B (en) * 2002-10-09 2006-04-05 Waters Investments Ltd Methods and apparatus for identifying compounds in a sample
WO2004035169A2 (fr) * 2002-10-15 2004-04-29 The Regents Of The University Of Michigan Systeme de separation multidimensionelle des proteines
US7648678B2 (en) 2002-12-20 2010-01-19 Dako Denmark A/S Method and system for pretreatment of tissue slides
WO2004063719A2 (fr) * 2003-01-13 2004-07-29 The Regents Of The University Of Michigan Systeme de jeu ordonne de microechantillons de proteines
US6969452B2 (en) * 2003-02-28 2005-11-29 Combisep, Inc. Two-dimensional protein separations using chromatofocusing and multiplexed capillary gel electrophoresis
US20040180449A1 (en) * 2003-03-11 2004-09-16 Gilson, Inc. Multidimensional chromatography with ion exchange solid phase extraction
GB2404194A (en) * 2003-07-21 2005-01-26 Amersham Biosciences Ab Automated chromatography/mass spectrometry analysis
GB2404193A (en) * 2003-07-21 2005-01-26 Amersham Biosciences Ab Automated chromatography/mass spectrometry analysis
CN1809753B (zh) * 2003-07-29 2010-10-20 索尔瓦药物有限公司 通过二维凝胶电泳分析包含生理可接受消化酶混合物的方法
FR2859113B1 (fr) * 2003-08-29 2006-02-17 Centre Nat Rech Scient Procede de separation bidimensionnelle par electrophorese capillaire realise dans un seul capillaire
KR100516389B1 (ko) * 2003-09-08 2005-09-23 한국과학기술원 작은 열 충격 단백질을 함유하는 단백질 분해 방지용조성물 및 이를 이용한 이차원 전기영동법
WO2006007853A2 (fr) 2004-07-20 2006-01-26 Symphogen A/S Procedure de caracterisation structurelle d'une proteine polyclonale recombinee ou d'une lignee de cellules polyclonales
BRPI0513706A (pt) 2004-07-20 2008-05-13 Symphogen As anticorpo policlonal recombinante anti-rhesus d e métodos de produção
US20080195062A1 (en) * 2005-06-08 2008-08-14 Vanderbilt University Sampling of blood analytes
US20090136960A1 (en) * 2006-03-24 2009-05-28 The Regents Of The University Of Michigan Methods and compositions for the identification of cancer markers
US8580935B2 (en) * 2007-02-26 2013-11-12 Alltech Associates, Inc. Ultra-fast chromatography
US20090069189A1 (en) * 2007-09-11 2009-03-12 Cancer Prevention And Cure, Ltd. Method of identifying proteins in human serum indicative of pathologies of human lung tissues
EP2234686A4 (fr) * 2007-11-30 2011-02-09 Waters Technologies Corp Procedes permettant de determiner la presence ou l'absence de mineralocorticoides dans des echantillons
US7834313B2 (en) * 2008-08-08 2010-11-16 Quest Diagnostics Investments Incorporated Mass spectrometry assay for plasma-renin
JP5569761B1 (ja) * 2013-03-29 2014-08-13 シャープ株式会社 分析方法
JP6127790B2 (ja) * 2013-07-12 2017-05-17 株式会社島津製作所 液体クロマトグラフ用制御装置および制御方法
CN105363426B (zh) * 2015-12-08 2018-10-16 复旦大学 一种介孔二氧化硅复合材料结合质谱鉴定肽段的方法
CN105536748B (zh) * 2015-12-09 2018-10-16 复旦大学 一种纳米复合材料结合质谱鉴定磷酸化肽段的方法
AU2018212974B2 (en) * 2017-01-30 2023-08-24 Regeneron Pharmaceuticals, Inc. Compositions and methods for reducing bioburden in chromatography
WO2021252990A1 (fr) * 2020-06-13 2021-12-16 Complete Omics Inc. Procédé et système d'analyse de néoantigènes

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US5451662A (en) * 1987-10-23 1995-09-19 Schering Corporation Method of purifying protein
EP0617048A1 (fr) * 1993-03-26 1994-09-28 Waters Investments Limited Méthode de focalisation isoélectrique en tube capillaire des protéines et peptides avec collection des fractions pour analyse après séparation
US5498545A (en) * 1994-07-21 1996-03-12 Vestal; Marvin L. Mass spectrometer system and method for matrix-assisted laser desorption measurements
EP0835446B1 (fr) * 1995-06-26 2003-04-09 Perseptive Biosystems, Inc. Selection et analyse moleculaires multidimensionnelles automatiques et rapides a flux continu
US5670054A (en) * 1996-04-04 1997-09-23 Warner Lambert Company Method and system for identification, purification, and quantitation of reaction components
EP0984978A4 (fr) * 1997-03-14 2002-01-09 Transgenomic Inc Affichage de separations polyonucleotidiques sous la forme d'un arrangement de bandes
US6534764B1 (en) * 1999-06-11 2003-03-18 Perseptive Biosystems Tandem time-of-flight mass spectrometer with damping in collision cell and method for use
US6545268B1 (en) * 2000-04-10 2003-04-08 Perseptive Biosystems Preparation of ion pulse for time-of-flight and for tandem time-of-flight mass analysis

Also Published As

Publication number Publication date
WO2001058925A2 (fr) 2001-08-16
JP2004500567A (ja) 2004-01-08
US20020098595A1 (en) 2002-07-25
EP1358202A2 (fr) 2003-11-05
MXPA02007664A (es) 2004-08-23
WO2001058925A3 (fr) 2003-09-04
CA2400484A1 (fr) 2001-08-16

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