ATE97443T1 - In vitro mutagenese. - Google Patents
In vitro mutagenese.Info
- Publication number
- ATE97443T1 ATE97443T1 AT90900787T AT90900787T ATE97443T1 AT E97443 T1 ATE97443 T1 AT E97443T1 AT 90900787 T AT90900787 T AT 90900787T AT 90900787 T AT90900787 T AT 90900787T AT E97443 T1 ATE97443 T1 AT E97443T1
- Authority
- AT
- Austria
- Prior art keywords
- nucleic acid
- primer
- insoluble support
- dna strand
- attached
- Prior art date
Links
- 238000000338 in vitro Methods 0.000 title abstract 2
- 238000002703 mutagenesis Methods 0.000 title abstract 2
- 231100000350 mutagenesis Toxicity 0.000 title abstract 2
- 108020004707 nucleic acids Proteins 0.000 abstract 6
- 102000039446 nucleic acids Human genes 0.000 abstract 6
- 150000007523 nucleic acids Chemical class 0.000 abstract 6
- 108020004414 DNA Proteins 0.000 abstract 5
- 102000053602 DNA Human genes 0.000 abstract 4
- 230000004075 alteration Effects 0.000 abstract 3
- 102100034343 Integrase Human genes 0.000 abstract 1
- 102000003960 Ligases Human genes 0.000 abstract 1
- 108090000364 Ligases Proteins 0.000 abstract 1
- 108020004711 Nucleic Acid Probes Proteins 0.000 abstract 1
- 108091034117 Oligonucleotide Proteins 0.000 abstract 1
- 108010092799 RNA-directed DNA polymerase Proteins 0.000 abstract 1
- 108020004682 Single-Stranded DNA Proteins 0.000 abstract 1
- 230000015572 biosynthetic process Effects 0.000 abstract 1
- 238000009396 hybridization Methods 0.000 abstract 1
- 239000007788 liquid Substances 0.000 abstract 1
- 239000006249 magnetic particle Substances 0.000 abstract 1
- 238000000034 method Methods 0.000 abstract 1
- 239000002853 nucleic acid probe Substances 0.000 abstract 1
- 239000002773 nucleotide Substances 0.000 abstract 1
- 125000003729 nucleotide group Chemical group 0.000 abstract 1
- 239000000523 sample Substances 0.000 abstract 1
- 238000003786 synthesis reaction Methods 0.000 abstract 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
- C12N15/102—Mutagenizing nucleic acids
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6813—Hybridisation assays
- C12Q1/6834—Enzymatic or biochemical coupling of nucleic acids to a solid phase
Landscapes
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Organic Chemistry (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Genetics & Genomics (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Biotechnology (AREA)
- General Engineering & Computer Science (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Analytical Chemistry (AREA)
- Biophysics (AREA)
- Molecular Biology (AREA)
- Biochemistry (AREA)
- Microbiology (AREA)
- Physics & Mathematics (AREA)
- General Health & Medical Sciences (AREA)
- Immunology (AREA)
- Biomedical Technology (AREA)
- Crystallography & Structural Chemistry (AREA)
- Plant Pathology (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Polysaccharides And Polysaccharide Derivatives (AREA)
- Solid-Sorbent Or Filter-Aiding Compositions (AREA)
- Saccharide Compounds (AREA)
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| GB888827167A GB8827167D0 (en) | 1988-11-21 | 1988-11-21 | In vitro mutagenesis |
| PCT/EP1989/001417 WO1990006043A2 (en) | 1988-11-21 | 1989-11-21 | In vitro mutagenesis |
| EP90900787A EP0448609B1 (de) | 1988-11-21 | 1989-11-21 | In vitro mutagenese |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| ATE97443T1 true ATE97443T1 (de) | 1993-12-15 |
Family
ID=10647193
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| AT90900787T ATE97443T1 (de) | 1988-11-21 | 1989-11-21 | In vitro mutagenese. |
Country Status (8)
| Country | Link |
|---|---|
| EP (1) | EP0448609B1 (de) |
| JP (1) | JPH04501957A (de) |
| AT (1) | ATE97443T1 (de) |
| AU (1) | AU628442B2 (de) |
| CA (1) | CA2003502A1 (de) |
| DE (1) | DE68910836T2 (de) |
| GB (1) | GB8827167D0 (de) |
| WO (1) | WO1990006043A2 (de) |
Families Citing this family (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| GB8926269D0 (en) * | 1989-11-21 | 1990-01-10 | Dynal As | Plasmid |
| US5712125A (en) * | 1990-07-24 | 1998-01-27 | Cemv Bioteknik Ab | Competitive PCR for quantitation of DNA |
| JPH08500722A (ja) * | 1992-01-29 | 1996-01-30 | 日立化成工業株式会社 | ポリヌクレオチド固定化担体 |
| US5591841A (en) * | 1993-01-14 | 1997-01-07 | Ji; Huamin | Rapid purification of circular DNA by triplex-mediated affinity capture |
| AU1811197A (en) * | 1996-02-25 | 1997-09-10 | Precision System Science Co., Ltd. | Method for treating biopolymers, microorganisms or materials by using more than one type of magnetic particles |
| EP0937497B9 (de) | 1997-12-25 | 2003-10-29 | Tosoh Corporation | Magnetischer Träger, seine Herstellung, und Verfahren zum Extrahieren von Nukleinsäuren |
| JP4735926B2 (ja) * | 2004-05-27 | 2011-07-27 | アイシン精機株式会社 | 核酸の高効率回収方法 |
Family Cites Families (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4863849A (en) * | 1985-07-18 | 1989-09-05 | New York Medical College | Automatable process for sequencing nucleotide |
| CA1338457C (en) * | 1986-08-22 | 1996-07-16 | Henry A. Erlich | Purified thermostable enzyme |
| ZA877772B (en) * | 1986-10-23 | 1988-04-20 | Amoco Corporation | Target and background capture methods and apparatus for affinity assays |
| US4942130A (en) * | 1987-01-14 | 1990-07-17 | President & Fellows Of Harvard College | T7 DNA polymerase |
-
1988
- 1988-11-21 GB GB888827167A patent/GB8827167D0/en active Pending
-
1989
- 1989-11-21 WO PCT/EP1989/001417 patent/WO1990006043A2/en not_active Ceased
- 1989-11-21 AT AT90900787T patent/ATE97443T1/de not_active IP Right Cessation
- 1989-11-21 EP EP90900787A patent/EP0448609B1/de not_active Expired - Lifetime
- 1989-11-21 AU AU46664/89A patent/AU628442B2/en not_active Ceased
- 1989-11-21 CA CA002003502A patent/CA2003502A1/en not_active Abandoned
- 1989-11-21 JP JP2501003A patent/JPH04501957A/ja active Pending
- 1989-11-21 DE DE90900787T patent/DE68910836T2/de not_active Expired - Fee Related
Also Published As
| Publication number | Publication date |
|---|---|
| EP0448609A1 (de) | 1991-10-02 |
| CA2003502A1 (en) | 1990-05-21 |
| DE68910836T2 (de) | 1994-04-28 |
| JPH04501957A (ja) | 1992-04-09 |
| AU4666489A (en) | 1990-06-26 |
| WO1990006043A3 (en) | 1990-11-01 |
| WO1990006043A2 (en) | 1990-06-14 |
| AU628442B2 (en) | 1992-09-17 |
| DE68910836D1 (de) | 1993-12-23 |
| EP0448609B1 (de) | 1993-11-18 |
| GB8827167D0 (en) | 1988-12-29 |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| UEP | Publication of translation of european patent specification | ||
| REN | Ceased due to non-payment of the annual fee |