ATE76103T1 - Integration eines gens in ein chromosom und dessen kontrollierter ausschnitt. - Google Patents

Integration eines gens in ein chromosom und dessen kontrollierter ausschnitt.

Info

Publication number
ATE76103T1
ATE76103T1 AT86301000T AT86301000T ATE76103T1 AT E76103 T1 ATE76103 T1 AT E76103T1 AT 86301000 T AT86301000 T AT 86301000T AT 86301000 T AT86301000 T AT 86301000T AT E76103 T1 ATE76103 T1 AT E76103T1
Authority
AT
Austria
Prior art keywords
gene
chromosome
vector
integration
excision
Prior art date
Application number
AT86301000T
Other languages
English (en)
Inventor
Ramaswamy Balakrishnan
Keith C Backman
Original Assignee
Bioteknika International
Heinz Co H J
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Bioteknika International, Heinz Co H J filed Critical Bioteknika International
Application granted granted Critical
Publication of ATE76103T1 publication Critical patent/ATE76103T1/de

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/67—General methods for enhancing the expression
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/52—Genes encoding for enzymes or proenzymes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
    • C12N15/90—Stable introduction of foreign DNA into chromosome
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P13/00—Preparation of nitrogen-containing organic compounds
    • C12P13/04—Alpha- or beta- amino acids
    • C12P13/22—Tryptophan; Tyrosine; Phenylalanine; 3,4-Dihydroxyphenylalanine
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10S435/00—Chemistry: molecular biology and microbiology
    • Y10S435/8215—Microorganisms
    • Y10S435/822—Microorganisms using bacteria or actinomycetales
    • Y10S435/848—Escherichia
    • Y10S435/849—Escherichia coli

Landscapes

  • Genetics & Genomics (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Plant Pathology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Mycology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Saccharide Compounds (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
AT86301000T 1985-02-13 1986-02-13 Integration eines gens in ein chromosom und dessen kontrollierter ausschnitt. ATE76103T1 (de)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US06/701,091 US4743546A (en) 1985-02-13 1985-02-13 Controlled gene excision
EP86301000A EP0191643B1 (de) 1985-02-13 1986-02-13 Integration eines Gens in ein Chromosom und dessen kontrollierter Ausschnitt

Publications (1)

Publication Number Publication Date
ATE76103T1 true ATE76103T1 (de) 1992-05-15

Family

ID=24816040

Family Applications (1)

Application Number Title Priority Date Filing Date
AT86301000T ATE76103T1 (de) 1985-02-13 1986-02-13 Integration eines gens in ein chromosom und dessen kontrollierter ausschnitt.

Country Status (6)

Country Link
US (1) US4743546A (de)
EP (1) EP0191643B1 (de)
JP (1) JPS61247389A (de)
AT (1) ATE76103T1 (de)
CA (1) CA1339799C (de)
DE (1) DE3685233D1 (de)

Families Citing this family (40)

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Publication number Priority date Publication date Assignee Title
US5169768A (en) * 1983-10-07 1992-12-08 Biotechnica International, Inc. Method of biosynthesis of phenylalanine
EP0263716A3 (de) * 1986-10-09 1988-12-28 Kyowa Hakko Kogyo Kabushiki Kaisha Verfahren zur Herstellung von 5'-guanilsäure und Expressionsvektoren zur Verwendung dazu
US5504005A (en) * 1987-03-02 1996-04-02 Albert Einstein College Of Medicine Of Yeshiva University Recombinant mycobacterial vaccine
FR2631634B1 (fr) * 1988-05-18 1991-06-14 Centre Nat Rech Scient Vecteurs de clonage et d'expression dans une souche d'actinomycete, procede de transformation de cette souche, souche d'actynomycete obtenue et preparation de proteines
HU200796B (en) * 1988-07-14 1990-08-28 Richter Gedeon Vegyeszet Process for producing expression vectors ensuring insertion of nucleotide sequences into e. coli chromosome
US5190871A (en) * 1989-06-12 1993-03-02 Eli Lilly And Company Use of the site-specific integrating function of phage φC31
US5856148A (en) * 1991-12-12 1999-01-05 Wacker Chemie Gmbh Materials and methods for biosynthesis of serine and serine-related products
TW313589B (de) * 1991-12-12 1997-08-21 Wacker Chemie Gmbh
US5834233A (en) * 1992-04-06 1998-11-10 Gx Biosystems A/S Method of limiting the survival of genetically engineered microorganisms in their enivronment
US6964861B1 (en) 1998-11-13 2005-11-15 Invitrogen Corporation Enhanced in vitro recombinational cloning of using ribosomal proteins
US6143557A (en) * 1995-06-07 2000-11-07 Life Technologies, Inc. Recombination cloning using engineered recombination sites
US6720140B1 (en) * 1995-06-07 2004-04-13 Invitrogen Corporation Recombinational cloning using engineered recombination sites
PT937098E (pt) 1995-06-07 2002-12-31 Invitrogen Corp Clonagem recombinatoria in vitro utilizando locais de recombinacao modificados
GB2304718B (en) * 1995-09-05 2000-01-19 Degussa The production of tryptophan by the bacterium escherichia coli
US5851808A (en) 1997-02-28 1998-12-22 Baylor College Of Medicine Rapid subcloning using site-specific recombination
US20030124555A1 (en) * 2001-05-21 2003-07-03 Invitrogen Corporation Compositions and methods for use in isolation of nucleic acid molecules
US7351578B2 (en) * 1999-12-10 2008-04-01 Invitrogen Corp. Use of multiple recombination sites with unique specificity in recombinational cloning
NZ520579A (en) * 1997-10-24 2004-08-27 Invitrogen Corp Recombinational cloning using nucleic acids having recombination sites and methods for synthesizing double stranded nucleic acids
EP2100963B1 (de) 1998-07-06 2011-12-14 DCV Inc. doing business as Bio-Technical Resourses Verfahren zur vitaminherstellung
EP2305825B1 (de) 1998-07-06 2015-01-14 DCV Inc. doing business as Bio-Technical Resourses Verfahren zur Vitaminherstellung
ATE443074T1 (de) 1999-03-02 2009-10-15 Life Technologies Corp Zubereitungen und methoden zur verwendung in rekombinatorischem klonen von nukleinsäuren
DK1250453T3 (da) 1999-12-10 2008-08-11 Invitrogen Corp Anvendelse af multiple rekombinations-sites med unik specificitet i rekombinationskloning
RU2314346C2 (ru) * 2000-04-06 2008-01-10 Киова Хакко Когио Со., Лтд Плазмиды для хромосомной рекомбинации escherichia coli
US7244560B2 (en) * 2000-05-21 2007-07-17 Invitrogen Corporation Methods and compositions for synthesis of nucleic acid molecules using multiple recognition sites
US7198924B2 (en) 2000-12-11 2007-04-03 Invitrogen Corporation Methods and compositions for synthesis of nucleic acid molecules using multiple recognition sites
TWI426127B (zh) 2001-04-16 2014-02-11 Dsm Ip Assets Bv 多不飽和脂肪酸（ｐｕｆａ）聚乙醯合成酶系統及其用途（三）
EP1390394A4 (de) * 2001-04-19 2004-05-26 Invitrogen Corp Zusammensetzungen und verfahren zur rekombinationsklonierung von nukleinsäuremolekülen
DE60333285D1 (de) 2002-05-14 2010-08-19 Martek Biosciences Corp Carotinsynthase-gen und dessen verwendung
US20040204953A1 (en) * 2002-07-17 2004-10-14 Lincoln Muir Subscription based systems, methods and components for providing genomic and proteomic products and services
US20050095615A1 (en) * 2003-06-26 2005-05-05 Welch Peter J. Methods and compositions for detecting promoter activity and expressing fusion proteins
JP2007512838A (ja) 2003-12-01 2007-05-24 インヴィトロジェン コーポレーション 組換え部位を含む核酸分子およびその使用方法
WO2006014837A1 (en) 2004-07-27 2006-02-09 The Regents Of The University Of California Genetically modified host cells and use of same for producing isoprenoid compounds
KR20140018851A (ko) 2010-11-24 2014-02-13 노파르티스 인터내셔널 파마슈티칼 리미티드 N-아세틸글루코사미닐트랜스퍼라제 활성을 갖는 융합 효소
US20140057323A1 (en) 2011-02-07 2014-02-27 The Board Of Trustees Of The University Of Illinois Enhanced cellodextrin metabolism
WO2013155481A1 (en) 2012-04-13 2013-10-17 The Board Of Trustees Of The University Of Illinois Improved cellodextrin transport and mixed sugar fermentation
DK2852610T3 (en) 2012-05-23 2018-09-03 Glykos Finland Oy PRODUCTION OF FUCOSYLED GLYCOPROTEIN
ES2739280T3 (es) 2013-07-04 2020-01-30 Glykos Finland Oy Células fúngicas filamentosas deficientes en O-manosiltransferasa y métodos de uso de las mismas
US20160153019A1 (en) 2013-07-10 2016-06-02 Novartis Ag Production of Glycoproteins Having Increased N-Glycosylation Site Occupancy
ES2930078T3 (es) 2016-05-12 2022-12-07 Dsm Ip Assets Bv Método para incrementar la producción de ácidos grasos poliinsaturados omega-3 en microalgas
CN116200322B (zh) * 2023-02-21 2026-01-02 江南大学 一种生产l-酪氨酸的重组大肠杆菌及应用

Family Cites Families (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4436815A (en) * 1981-11-27 1984-03-13 Eli Lilly And Company Method for stabilizing and selecting recombinant DNA containing host cells
BG49718A3 (bg) * 1983-07-15 1992-01-15 Bio- Technology General Corp Метод за получаване на полипептид със супероксиддисмутазна активност

Also Published As

Publication number Publication date
DE3685233D1 (de) 1992-06-17
EP0191643A2 (de) 1986-08-20
CA1339799C (en) 1998-04-07
JPS61247389A (ja) 1986-11-04
EP0191643A3 (en) 1988-11-02
EP0191643B1 (de) 1992-05-13
US4743546A (en) 1988-05-10

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