ATE550433T1 - Dna-kloniervektorplasmide sowie verfahren zu deren verwendung - Google Patents
Dna-kloniervektorplasmide sowie verfahren zu deren verwendungInfo
- Publication number
- ATE550433T1 ATE550433T1 AT09013246T AT09013246T ATE550433T1 AT E550433 T1 ATE550433 T1 AT E550433T1 AT 09013246 T AT09013246 T AT 09013246T AT 09013246 T AT09013246 T AT 09013246T AT E550433 T1 ATE550433 T1 AT E550433T1
- Authority
- AT
- Austria
- Prior art keywords
- cloning vector
- vector plasmids
- cloning
- gene expression
- dna cloning
- Prior art date
Links
- 239000013612 plasmid Substances 0.000 title abstract 4
- 239000013599 cloning vector Substances 0.000 title abstract 3
- 238000000034 method Methods 0.000 title abstract 3
- 108700019146 Transgenes Proteins 0.000 abstract 3
- 238000010367 cloning Methods 0.000 abstract 2
- 230000014509 gene expression Effects 0.000 abstract 2
- 239000013598 vector Substances 0.000 abstract 2
- 241000894006 Bacteria Species 0.000 abstract 1
- 241000206602 Eukaryota Species 0.000 abstract 1
- 241000699670 Mus sp. Species 0.000 abstract 1
- 240000004808 Saccharomyces cerevisiae Species 0.000 abstract 1
- 239000012634 fragment Substances 0.000 abstract 1
- 238000012986 modification Methods 0.000 abstract 1
- 230000004048 modification Effects 0.000 abstract 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
Landscapes
- Genetics & Genomics (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Wood Science & Technology (AREA)
- Organic Chemistry (AREA)
- Biotechnology (AREA)
- General Engineering & Computer Science (AREA)
- Biomedical Technology (AREA)
- Zoology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Molecular Biology (AREA)
- Microbiology (AREA)
- Plant Pathology (AREA)
- Physics & Mathematics (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- Biophysics (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Saccharide Compounds (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US10/682,764 US7785871B2 (en) | 2002-10-09 | 2003-10-09 | DNA cloning vector plasmids and methods for their use |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| ATE550433T1 true ATE550433T1 (de) | 2012-04-15 |
Family
ID=34520551
Family Applications (2)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| AT09013246T ATE550433T1 (de) | 2003-10-09 | 2004-05-04 | Dna-kloniervektorplasmide sowie verfahren zu deren verwendung |
| AT04751078T ATE468399T1 (de) | 2003-10-09 | 2004-05-04 | Dna-kloniervektorplasmide sowie verfahren zu deren verwendung |
Family Applications After (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| AT04751078T ATE468399T1 (de) | 2003-10-09 | 2004-05-04 | Dna-kloniervektorplasmide sowie verfahren zu deren verwendung |
Country Status (15)
| Country | Link |
|---|---|
| US (2) | US7785871B2 (de) |
| EP (3) | EP2270180B1 (de) |
| JP (1) | JP4611307B2 (de) |
| CN (1) | CN1890373B (de) |
| AT (2) | ATE550433T1 (de) |
| AU (1) | AU2004284374B2 (de) |
| CA (1) | CA2542053C (de) |
| DE (1) | DE602004027293D1 (de) |
| DK (2) | DK2141238T3 (de) |
| ES (2) | ES2384296T3 (de) |
| NZ (1) | NZ546809A (de) |
| PL (1) | PL1692290T3 (de) |
| PT (1) | PT1692290E (de) |
| SI (1) | SI1692290T1 (de) |
| WO (1) | WO2005040336A2 (de) |
Families Citing this family (31)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US7785871B2 (en) * | 2002-10-09 | 2010-08-31 | Intrexon Corporation | DNA cloning vector plasmids and methods for their use |
| US20080050808A1 (en) * | 2002-10-09 | 2008-02-28 | Reed Thomas D | DNA modular cloning vector plasmids and methods for their use |
| EP2484772B1 (de) * | 2004-05-18 | 2016-08-17 | Intrexon Corporation | Verfahren zur dynamischen Vektorassemblierung zur DNA-Klonierung von Vektorplasmiden |
| US8202727B2 (en) * | 2005-05-17 | 2012-06-19 | Ozgene Pty Ltd. | Sequential cloning system |
| US8153598B2 (en) * | 2005-10-19 | 2012-04-10 | Intrexon Corporation | PKD ligands and polynucleotides encoding PKD ligands |
| US20070190536A1 (en) * | 2006-02-16 | 2007-08-16 | Academia Sinica | Method for using biomaterials as reagent for nano-patterning |
| US7943732B2 (en) | 2006-06-05 | 2011-05-17 | Intrexon Corporation | AKT ligands and polynucleotides encoding AKT ligands |
| KR20090035011A (ko) | 2006-07-26 | 2009-04-08 | 인트렉손 코포레이션 | 질병을 치료하는 방법 및 조성물 |
| US8993263B2 (en) * | 2006-08-07 | 2015-03-31 | Intrexon Corporation | PKA ligands and polynucleotides encoding PKA ligands |
| US8999666B2 (en) * | 2006-08-09 | 2015-04-07 | Intrexon Corporation | PKC ligands and polynucleotides encoding PKC ligands |
| US7897394B2 (en) * | 2006-09-21 | 2011-03-01 | Intrexon Corporation | Endoplasmic reticulum localization signals |
| US8729225B2 (en) | 2006-11-13 | 2014-05-20 | Intrexon Corporation | Glycogen synthase kinase heteropolyligand polypeptide |
| US8283445B2 (en) | 2006-11-13 | 2012-10-09 | Intrexon Corporation | Extracellular-signal-regulated-kinase (ERK) heteropolyligand polypeptide |
| US7705122B2 (en) | 2006-12-04 | 2010-04-27 | Intrexon Corporation | mTOR ligands and polynucleotides encoding mTOR ligands |
| US20090181458A1 (en) * | 2006-12-04 | 2009-07-16 | Thomas David Reed | Tubulo-vesicular structure localization signals |
| CN101679956A (zh) | 2007-03-27 | 2010-03-24 | 英特拉克森公司 | Mek配体和编码mek配体的多核苷酸 |
| EP2559707A3 (de) | 2007-05-02 | 2013-05-22 | Intrexon Corporation | PP1-Liganden |
| JP2010536373A (ja) | 2007-08-23 | 2010-12-02 | イントレキソン コーポレーション | 疾患を診断するための方法および組成物 |
| WO2009042971A2 (en) | 2007-09-26 | 2009-04-02 | Intrexon Corporation | Synthetic 5'utrs, expression vectors, and methods for increasing transgene expression |
| US20090136465A1 (en) | 2007-09-28 | 2009-05-28 | Intrexon Corporation | Therapeutic Gene-Switch Constructs and Bioreactors for the Expression of Biotherapeutic Molecules, and Uses Thereof |
| BRPI0818287A2 (pt) * | 2007-10-08 | 2015-11-03 | Intrexon Corp | células dendríticas manipuladas e usos para o tratamento do câncer. |
| EP2242501A4 (de) * | 2008-01-09 | 2011-10-19 | Intrexon Corp | Therapeutische hemmer der pai-1-funktion, verfahren zu ihrer verwendung |
| CA2754161A1 (en) | 2009-03-06 | 2010-09-10 | Europaisches Laboratorium Fur Molekularbiologie (Embl) | Nucleic acids for cloning and expressing multiprotein complexes |
| CN107043408B (zh) | 2009-06-09 | 2021-02-09 | 儿童医院医疗中心 | 抗原-诺罗病毒p-结构域单体和二聚体,抗原-诺罗病毒p-粒子分子,及其制备和应用方法 |
| KR20130010121A (ko) | 2010-03-23 | 2013-01-25 | 인트렉손 코포레이션 | 치료적 단백질을 조건부로 발현하는 벡터,상기 벡터를 포함하는 숙주 세포 및 이의 용도 |
| WO2012122025A2 (en) | 2011-03-04 | 2012-09-13 | Intrexon Corporation | Vectors conditionally expressing protein |
| GB2496849B (en) * | 2011-11-19 | 2016-06-08 | Oxford Genetics Ltd | Improved DNA plasmid system |
| US10829772B2 (en) | 2012-06-22 | 2020-11-10 | Monsanto Technology Llc | Unique modular vector design |
| US9321803B2 (en) | 2013-07-12 | 2016-04-26 | Children's Hospital Medical Center | Compositions and methods for inhibiting norovirus infection |
| CA3042706A1 (en) | 2016-11-09 | 2018-05-17 | Intrexon Corporation | Frataxin expression constructs |
| JP7641695B2 (ja) | 2017-03-28 | 2025-03-07 | チルドレンズ ホスピタル メディカル センター | ノロウイルスs粒子ベースのワクチンならびにその作製および使用方法 |
Family Cites Families (26)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4661454A (en) * | 1983-02-28 | 1987-04-28 | Collaborative Research, Inc. | GAL1 yeast promoter linked to non galactokinase gene |
| US4820642A (en) * | 1983-04-04 | 1989-04-11 | The Regents Of The University Of California | Amplified expression vector |
| DE3740046A1 (de) * | 1987-10-23 | 1989-05-03 | Kotterer Grafotec | Verfahren und vorrichtung zum falzen einer papierbahn |
| JPH0797997B2 (ja) * | 1988-04-28 | 1995-10-25 | 帝人株式会社 | 新規生理活性ポリペプチド |
| US5061628A (en) * | 1989-07-18 | 1991-10-29 | Rutgers University | Restriction endonuclease FseI |
| US5192676A (en) * | 1991-02-05 | 1993-03-09 | New England Biolabs, Inc. | Type ii restriction endonuclease, asci, obtainable from arthrobacter species and a process for producing the same |
| US5223408A (en) * | 1991-07-11 | 1993-06-29 | Genentech, Inc. | Method for making variant secreted proteins with altered properties |
| US6042828A (en) * | 1992-09-07 | 2000-03-28 | Kyowa Hakko Kogyo Co., Ltd. | Humanized antibodies to ganglioside GM2 |
| US5787104A (en) * | 1995-01-19 | 1998-07-28 | Matsushita Electric Industrial Co., Ltd. | Semiconductor light emitting element and method for fabricating the same |
| US5919667A (en) * | 1996-06-20 | 1999-07-06 | The Salk Institute For Biological Studies | Modular assembly retroviral vectors and uses thereof |
| US6248569B1 (en) | 1997-11-10 | 2001-06-19 | Brookhaven Science Associates | Method for introducing unidirectional nested deletions |
| US6096523A (en) * | 1998-11-04 | 2000-08-01 | University Of Georgia Research Foundation | Transformation vector system |
| US6562624B2 (en) * | 1999-03-17 | 2003-05-13 | Paradigm Genetics, Inc. | Methods and materials for the rapid and high volume production of a gene knock-out library in an organism |
| JP2002540772A (ja) * | 1999-03-24 | 2002-12-03 | ボード オブ リージェンツ, ザ ユニバーシティ オブ テキサス システム | 線状および環状発現エレメント |
| US6245545B1 (en) * | 1999-04-27 | 2001-06-12 | New England Biolabs, Inc. | Method for cloning and producing the SwaI restriction endonuclease |
| US6184000B1 (en) | 1999-07-23 | 2001-02-06 | The United States Of America As Represented By The Secretary Of Agriculture | System for the sequential, directional cloning of multiple DNA sequences |
| ES2316461T3 (es) * | 2000-06-28 | 2009-04-16 | Sungene Gmbh | Vectores binarios para la transformacion mejorada de sistemas vegetales. |
| FR2825103B1 (fr) * | 2001-05-28 | 2003-09-19 | Genoway | Vecteurs de clonage pour recombinaison homologue et procede les utilisant |
| US6514737B1 (en) * | 2001-08-20 | 2003-02-04 | New England Biolabs, Inc. | Method for cloning and expression of AsiSI restriction endonuclease and AsiSI methylase in E. coli |
| CN1263860C (zh) | 2002-09-30 | 2006-07-12 | 华南农业大学 | 多基因载体的构建方法与应用 |
| US20080050808A1 (en) * | 2002-10-09 | 2008-02-28 | Reed Thomas D | DNA modular cloning vector plasmids and methods for their use |
| US7785871B2 (en) * | 2002-10-09 | 2010-08-31 | Intrexon Corporation | DNA cloning vector plasmids and methods for their use |
| US8293503B2 (en) * | 2003-10-03 | 2012-10-23 | Promega Corporation | Vectors for directional cloning |
| ATE514783T1 (de) * | 2003-11-12 | 2011-07-15 | Schering Corp | Plasmidsystem zur expression mehrerer gene |
| EP2484772B1 (de) * | 2004-05-18 | 2016-08-17 | Intrexon Corporation | Verfahren zur dynamischen Vektorassemblierung zur DNA-Klonierung von Vektorplasmiden |
| US8153598B2 (en) * | 2005-10-19 | 2012-04-10 | Intrexon Corporation | PKD ligands and polynucleotides encoding PKD ligands |
-
2003
- 2003-10-09 US US10/682,764 patent/US7785871B2/en not_active Expired - Fee Related
-
2004
- 2004-05-04 PT PT04751078T patent/PT1692290E/pt unknown
- 2004-05-04 JP JP2006532528A patent/JP4611307B2/ja not_active Expired - Fee Related
- 2004-05-04 DE DE602004027293T patent/DE602004027293D1/de not_active Expired - Lifetime
- 2004-05-04 SI SI200431477T patent/SI1692290T1/sl unknown
- 2004-05-04 AT AT09013246T patent/ATE550433T1/de active
- 2004-05-04 ES ES09013246T patent/ES2384296T3/es not_active Expired - Lifetime
- 2004-05-04 DK DK09013246.5T patent/DK2141238T3/da active
- 2004-05-04 EP EP10181449.9A patent/EP2270180B1/de not_active Expired - Lifetime
- 2004-05-04 CN CN2004800367922A patent/CN1890373B/zh not_active Expired - Fee Related
- 2004-05-04 CA CA2542053A patent/CA2542053C/en not_active Expired - Fee Related
- 2004-05-04 DK DK04751078.9T patent/DK1692290T3/da active
- 2004-05-04 WO PCT/US2004/013517 patent/WO2005040336A2/en not_active Ceased
- 2004-05-04 EP EP09013246A patent/EP2141238B1/de not_active Expired - Lifetime
- 2004-05-04 PL PL04751078T patent/PL1692290T3/pl unknown
- 2004-05-04 AT AT04751078T patent/ATE468399T1/de active
- 2004-05-04 NZ NZ546809A patent/NZ546809A/en not_active IP Right Cessation
- 2004-05-04 AU AU2004284374A patent/AU2004284374B2/en not_active Ceased
- 2004-05-04 EP EP04751078A patent/EP1692290B1/de not_active Expired - Lifetime
- 2004-05-04 ES ES04751078T patent/ES2346219T3/es not_active Expired - Lifetime
-
2007
- 2007-08-17 US US11/840,297 patent/US20080241915A1/en not_active Abandoned
Also Published As
| Publication number | Publication date |
|---|---|
| EP2141238B1 (de) | 2012-03-21 |
| CA2542053C (en) | 2013-10-29 |
| DK1692290T3 (da) | 2010-09-06 |
| EP2270180A2 (de) | 2011-01-05 |
| ES2346219T3 (es) | 2010-10-13 |
| PT1692290E (pt) | 2010-08-17 |
| US20040185556A1 (en) | 2004-09-23 |
| CA2542053A1 (en) | 2005-05-06 |
| EP2270180A3 (de) | 2011-04-20 |
| WO2005040336A2 (en) | 2005-05-06 |
| ATE468399T1 (de) | 2010-06-15 |
| DE602004027293D1 (de) | 2010-07-01 |
| EP1692290B1 (de) | 2010-05-19 |
| EP2270180B1 (de) | 2013-08-07 |
| SI1692290T1 (sl) | 2010-10-29 |
| US7785871B2 (en) | 2010-08-31 |
| CN1890373A (zh) | 2007-01-03 |
| NZ546809A (en) | 2008-08-29 |
| EP1692290A4 (de) | 2007-02-21 |
| CN1890373B (zh) | 2011-12-07 |
| US20080241915A1 (en) | 2008-10-02 |
| WO2005040336A3 (en) | 2005-09-29 |
| PL1692290T3 (pl) | 2010-10-29 |
| EP1692290A2 (de) | 2006-08-23 |
| DK2141238T3 (da) | 2012-07-09 |
| JP4611307B2 (ja) | 2011-01-12 |
| WO2005040336B1 (en) | 2005-12-01 |
| ES2384296T3 (es) | 2012-07-03 |
| AU2004284374B2 (en) | 2010-11-11 |
| JP2007508009A (ja) | 2007-04-05 |
| EP2141238A1 (de) | 2010-01-06 |
| AU2004284374A1 (en) | 2005-05-06 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| ATE468399T1 (de) | Dna-kloniervektorplasmide sowie verfahren zu deren verwendung | |
| Smith et al. | Generating a synthetic genome by whole genome assembly: φX174 bacteriophage from synthetic oligonucleotides | |
| WO2007038276A3 (en) | Dna modular cloning vector plasmids and methods for their use | |
| WO2005081714A3 (en) | Use of interfering rna in the production of transgenic animals | |
| DE602008005920D1 (en) | Gene | |
| ATE537254T1 (de) | Mutanter transposonvektor und verwendung davon | |
| ATE469231T1 (de) | Von rna-virus abgeleitetes pflanzenexpressionssystem | |
| DE60335574D1 (de) | Verfahren zur biologischen herstellung von 1,3-propandiol mit hoher ausbeute | |
| ATE412054T1 (de) | Delta-endotoxin gene und verfahren zu ihrer verwendung | |
| NO20013371L (no) | Ekspresjon og eksport av anti-fedmeproteiner som Fc- fusjonsproteiner | |
| ATE238425T1 (de) | Selektionmarker-genfreie rekombinante stämme: verfahren zur ihrer herstellung und die verwendung dieser stämme | |
| ATE506443T1 (de) | Verwendung einer nukleinsäure zur herstellung einer transgenen pflanze mit verbesserter toleranz gegenüber trockenheit | |
| ATE469984T1 (de) | Rekombinationsstellen enthaltende nukleinsäuremoleküle und verfahren zur verwendung davon | |
| Mengiste et al. | MutaT7GDE: A single chimera for the targeted, balanced, efficient, and processive installation of all possible transition mutations in vivo | |
| EP1859046A4 (de) | Expressionsvektor für tierische zelle mit wenigstens einer kopie von mar-dna-sequenzen am 3'-ende des transkriptionsterminationsbereichs eines gens sowie verfahren zur expression eines fremdgens unter verwendung des vektors | |
| WO2004013333A3 (de) | Revertierung der negativ-selektiven wirkung von negativen markerproteinen als selektionsverfahren | |
| WO2002098455A3 (en) | Production of recombinant human arylsulfatase a | |
| Wei et al. | Rapid gene splicing and multi-sited mutagenesis by one-step overlap extension polymerase chain reaction | |
| ATE393574T1 (de) | Zur glucoseregulierten produktion von menschlichem insulin in somatischen zelllinien geeignete nukleinsäurekonstrukte | |
| WO2006000319A3 (de) | Verfahren zur erhöhung der pathogenresistenz in transgenen pflanzen durch expression einer peroxidase | |
| EP1728869A3 (de) | Gen-Expressionskonstrukt von Hap1 modifiziert für Planzenzellen | |
| WO2003040291A3 (de) | Gene die für kohlenstoffmetabolismus- und energieproduktion-proteine codieren | |
| EP1788075A4 (de) | Grosstechnisch geeigneter mikroorganismus | |
| WO2003040289A3 (de) | Gene die für dna-replikations- und pathogenese proteine codieren | |
| Gupta et al. | Codon optimization |