ATE536408T1 - MODIFIED POLYNUCLEOTIDES FOR USE IN RNA INTERFERENCE - Google Patents

MODIFIED POLYNUCLEOTIDES FOR USE IN RNA INTERFERENCE

Info

Publication number
ATE536408T1
ATE536408T1 AT04749718T AT04749718T ATE536408T1 AT E536408 T1 ATE536408 T1 AT E536408T1 AT 04749718 T AT04749718 T AT 04749718T AT 04749718 T AT04749718 T AT 04749718T AT E536408 T1 ATE536408 T1 AT E536408T1
Authority
AT
Austria
Prior art keywords
rna interference
terminal
modifications
modified polynucleotides
nucleotides
Prior art date
Application number
AT04749718T
Other languages
German (de)
Inventor
Devin Leake
Angela Reynolds
Anastasia Khvorova
William Marshall
Yuriy Fedorov
Kimberly Nichols
Original Assignee
Dharmacon Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from US10/406,908 external-priority patent/US20040198640A1/en
Application filed by Dharmacon Inc filed Critical Dharmacon Inc
Priority claimed from PCT/US2004/010343 external-priority patent/WO2004090105A2/en
Application granted granted Critical
Publication of ATE536408T1 publication Critical patent/ATE536408T1/en

Links

Abstract

<P>PROBLEM TO BE SOLVED: To provide a modification for an siRNA capable of increasing or decreasing specificity and/or stability. <P>SOLUTION: There are provided a method and a composition for performing RNA interference comprising a wide variety of stabilized siRNAs suitable for use in serum-containing media and for in vivo applications (such as therapeutic applications). Uniquely modified siRNAs have been developed that reduce off-target effects incurred in gene-silencing. The modifications include phosphorylation of the first 5' terminal antisense nucleotide; 2' carbon modifications of the first and second or first, second and third 5' terminal antisense nucleotides; and optionally 2' carbon modifications of the first and second or first, second and third 5' terminal sense nucleotides. Control and exaequo molecules are also provided. <P>COPYRIGHT: (C)2010,JPO&INPIT
AT04749718T 2003-04-02 2004-04-01 MODIFIED POLYNUCLEOTIDES FOR USE IN RNA INTERFERENCE ATE536408T1 (en)

Applications Claiming Priority (7)

Application Number Priority Date Filing Date Title
US10/406,908 US20040198640A1 (en) 2003-04-02 2003-04-02 Stabilized polynucleotides for use in RNA interference
US10/613,077 US20040266707A1 (en) 2003-04-02 2003-07-01 Stabilized polynucleotides for use in RNA interference
US54264604P 2004-02-06 2004-02-06
US54266804P 2004-02-06 2004-02-06
US54364004P 2004-02-10 2004-02-10
US54366104P 2004-02-10 2004-02-10
PCT/US2004/010343 WO2004090105A2 (en) 2003-04-02 2004-04-01 Modified polynucleotides for use in rna interference

Publications (1)

Publication Number Publication Date
ATE536408T1 true ATE536408T1 (en) 2011-12-15

Family

ID=42703870

Family Applications (1)

Application Number Title Priority Date Filing Date
AT04749718T ATE536408T1 (en) 2003-04-02 2004-04-01 MODIFIED POLYNUCLEOTIDES FOR USE IN RNA INTERFERENCE

Country Status (2)

Country Link
JP (1) JP5468978B2 (en)
AT (1) ATE536408T1 (en)

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
AR083445A1 (en) 2010-10-14 2013-02-27 Univ Mie siRNA AGAINST FIBROSIS

Family Cites Families (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DK2813582T3 (en) * 2000-12-01 2017-07-31 Max-Planck-Gesellschaft Zur Förderung Der Wss E V Small RNA molecules that mediate RNA interference
AU2002329667A1 (en) * 2001-07-30 2003-02-17 Uta Griesenbach Specific inhibition of gene expression by small double stranded rnas
SI3222724T1 (en) * 2002-08-05 2019-03-29 Silence Therapeutics Gmbh Further novel forms of interfering rna molecules

Also Published As

Publication number Publication date
JP2010172344A (en) 2010-08-12
JP5468978B2 (en) 2014-04-09

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