ATE536408T1 - MODIFIED POLYNUCLEOTIDES FOR USE IN RNA INTERFERENCE - Google Patents
MODIFIED POLYNUCLEOTIDES FOR USE IN RNA INTERFERENCEInfo
- Publication number
- ATE536408T1 ATE536408T1 AT04749718T AT04749718T ATE536408T1 AT E536408 T1 ATE536408 T1 AT E536408T1 AT 04749718 T AT04749718 T AT 04749718T AT 04749718 T AT04749718 T AT 04749718T AT E536408 T1 ATE536408 T1 AT E536408T1
- Authority
- AT
- Austria
- Prior art keywords
- rna interference
- terminal
- modifications
- modified polynucleotides
- nucleotides
- Prior art date
Links
Abstract
<P>PROBLEM TO BE SOLVED: To provide a modification for an siRNA capable of increasing or decreasing specificity and/or stability. <P>SOLUTION: There are provided a method and a composition for performing RNA interference comprising a wide variety of stabilized siRNAs suitable for use in serum-containing media and for in vivo applications (such as therapeutic applications). Uniquely modified siRNAs have been developed that reduce off-target effects incurred in gene-silencing. The modifications include phosphorylation of the first 5' terminal antisense nucleotide; 2' carbon modifications of the first and second or first, second and third 5' terminal antisense nucleotides; and optionally 2' carbon modifications of the first and second or first, second and third 5' terminal sense nucleotides. Control and exaequo molecules are also provided. <P>COPYRIGHT: (C)2010,JPO&INPIT
Applications Claiming Priority (7)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US10/406,908 US20040198640A1 (en) | 2003-04-02 | 2003-04-02 | Stabilized polynucleotides for use in RNA interference |
US10/613,077 US20040266707A1 (en) | 2003-04-02 | 2003-07-01 | Stabilized polynucleotides for use in RNA interference |
US54264604P | 2004-02-06 | 2004-02-06 | |
US54266804P | 2004-02-06 | 2004-02-06 | |
US54364004P | 2004-02-10 | 2004-02-10 | |
US54366104P | 2004-02-10 | 2004-02-10 | |
PCT/US2004/010343 WO2004090105A2 (en) | 2003-04-02 | 2004-04-01 | Modified polynucleotides for use in rna interference |
Publications (1)
Publication Number | Publication Date |
---|---|
ATE536408T1 true ATE536408T1 (en) | 2011-12-15 |
Family
ID=42703870
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
AT04749718T ATE536408T1 (en) | 2003-04-02 | 2004-04-01 | MODIFIED POLYNUCLEOTIDES FOR USE IN RNA INTERFERENCE |
Country Status (2)
Country | Link |
---|---|
JP (1) | JP5468978B2 (en) |
AT (1) | ATE536408T1 (en) |
Families Citing this family (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
AR083445A1 (en) | 2010-10-14 | 2013-02-27 | Univ Mie | siRNA AGAINST FIBROSIS |
Family Cites Families (3)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
DK2813582T3 (en) * | 2000-12-01 | 2017-07-31 | Max-Planck-Gesellschaft Zur Förderung Der Wss E V | Small RNA molecules that mediate RNA interference |
AU2002329667A1 (en) * | 2001-07-30 | 2003-02-17 | Uta Griesenbach | Specific inhibition of gene expression by small double stranded rnas |
SI3222724T1 (en) * | 2002-08-05 | 2019-03-29 | Silence Therapeutics Gmbh | Further novel forms of interfering rna molecules |
-
2004
- 2004-04-01 AT AT04749718T patent/ATE536408T1/en active
-
2010
- 2010-04-30 JP JP2010106021A patent/JP5468978B2/en not_active Expired - Lifetime
Also Published As
Publication number | Publication date |
---|---|
JP2010172344A (en) | 2010-08-12 |
JP5468978B2 (en) | 2014-04-09 |
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