ATE481635T1 - Verfahren zur analyse des intrazellulären metabolischen flusses unter verwendung eines mit isotop markierten substrates - Google Patents

Verfahren zur analyse des intrazellulären metabolischen flusses unter verwendung eines mit isotop markierten substrates

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Publication number
ATE481635T1
ATE481635T1 AT05002402T AT05002402T ATE481635T1 AT E481635 T1 ATE481635 T1 AT E481635T1 AT 05002402 T AT05002402 T AT 05002402T AT 05002402 T AT05002402 T AT 05002402T AT E481635 T1 ATE481635 T1 AT E481635T1
Authority
AT
Austria
Prior art keywords
metabolic flux
labeled substrate
isotope
medium
isotop
Prior art date
Application number
AT05002402T
Other languages
English (en)
Inventor
Shintaro Iwatani
Yoshihiro Usuda
Kazuhiko Matsui
Original Assignee
Ajinomoto Kk
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Publication date
Application filed by Ajinomoto Kk filed Critical Ajinomoto Kk
Application granted granted Critical
Publication of ATE481635T1 publication Critical patent/ATE481635T1/de

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    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/5005—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells
    • G01N33/5091—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing the pathological state of an organism
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • G—PHYSICS
    • G16—INFORMATION AND COMMUNICATION TECHNOLOGY [ICT] SPECIALLY ADAPTED FOR SPECIFIC APPLICATION FIELDS
    • G16B—BIOINFORMATICS, i.e. INFORMATION AND COMMUNICATION TECHNOLOGY [ICT] SPECIALLY ADAPTED FOR GENETIC OR PROTEIN-RELATED DATA PROCESSING IN COMPUTATIONAL MOLECULAR BIOLOGY
    • G16B5/00—ICT specially adapted for modelling or simulations in systems biology, e.g. gene-regulatory networks, protein interaction networks or metabolic networks
    • G—PHYSICS
    • G16—INFORMATION AND COMMUNICATION TECHNOLOGY [ICT] SPECIALLY ADAPTED FOR SPECIFIC APPLICATION FIELDS
    • G16B—BIOINFORMATICS, i.e. INFORMATION AND COMMUNICATION TECHNOLOGY [ICT] SPECIALLY ADAPTED FOR GENETIC OR PROTEIN-RELATED DATA PROCESSING IN COMPUTATIONAL MOLECULAR BIOLOGY
    • G16B5/00—ICT specially adapted for modelling or simulations in systems biology, e.g. gene-regulatory networks, protein interaction networks or metabolic networks
    • G16B5/30—Dynamic-time models

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Physics & Mathematics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Molecular Biology (AREA)
  • Immunology (AREA)
  • Biotechnology (AREA)
  • General Health & Medical Sciences (AREA)
  • Physiology (AREA)
  • Organic Chemistry (AREA)
  • Biophysics (AREA)
  • Zoology (AREA)
  • Hematology (AREA)
  • Spectroscopy & Molecular Physics (AREA)
  • Analytical Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Medical Informatics (AREA)
  • Microbiology (AREA)
  • Evolutionary Biology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Biochemistry (AREA)
  • Urology & Nephrology (AREA)
  • Theoretical Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Cell Biology (AREA)
  • Genetics & Genomics (AREA)
  • General Engineering & Computer Science (AREA)
  • Food Science & Technology (AREA)
  • Medicinal Chemistry (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
AT05002402T 2004-02-05 2005-02-04 Verfahren zur analyse des intrazellulären metabolischen flusses unter verwendung eines mit isotop markierten substrates ATE481635T1 (de)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
JP2004029376 2004-02-05

Publications (1)

Publication Number Publication Date
ATE481635T1 true ATE481635T1 (de) 2010-10-15

Family

ID=34675528

Family Applications (1)

Application Number Title Priority Date Filing Date
AT05002402T ATE481635T1 (de) 2004-02-05 2005-02-04 Verfahren zur analyse des intrazellulären metabolischen flusses unter verwendung eines mit isotop markierten substrates

Country Status (4)

Country Link
US (1) US8510054B2 (de)
EP (1) EP1562044B1 (de)
AT (1) ATE481635T1 (de)
DE (1) DE602005023552D1 (de)

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JP2005515452A (ja) * 2001-10-24 2005-05-26 ザ リージェンツ オブ ザ ユニバーシティ オブ カリフォルニア アイソトープで標識された水を用いるヒトおよび実験系におけるタンパク質合成速度の測定
CA2475924C (en) 2002-02-12 2016-03-29 The Regents Of The University Of California Non-invasive method for measuring rates of biosynthesis of biological molecules by label incorporation
EP1546364A4 (de) 2002-07-30 2006-09-06 Univ California Verfahren zur automatischen messung im grossmassstab der molekularen flussgeschwindigkeiten des proteoms oder des organoms mittels massenspektrometrie
WO2004021863A2 (en) * 2002-09-04 2004-03-18 The Regents Of The University Of California Methods for measuring rates of replication and death of nfectious microbial agents in an infected host organism
CA2498378C (en) * 2002-09-13 2013-12-17 The Regents Of The University Of California Methods for measuring rates of reverse cholesterol transport in vivo, as an index of anti-atherogenesis
US20070248540A1 (en) * 2002-09-16 2007-10-25 The Regents Of The University Of California Biochemical methods for measuring metabolic fitness of tissues or whole organisms
AU2003291731B2 (en) 2002-11-04 2009-09-10 The Regents Of The University Of California Deuterated glucose or fat tolerance tests for high-throughput measurement of the metabolism of sugars or fatty acids in the body
US7262020B2 (en) * 2003-07-03 2007-08-28 The Regents Of The University Of California Methods for comparing relative flux rates of two or more biological molecules in vivo through a single protocol
US20050202406A1 (en) * 2003-11-25 2005-09-15 The Regents Of The University Of California Method for high-throughput screening of compounds and combinations of compounds for discovery and quantification of actions, particularly unanticipated therapeutic or toxic actions, in biological systems
TW200538738A (en) 2004-02-20 2005-12-01 Univ California Molecular flux rates through critical pathways measured by stable isotope labeling in vivo, as biomarkers of drug action and disease activity
WO2005087943A1 (en) * 2004-03-11 2005-09-22 The Regents Of The University Of California Temporal or spatial characterization of biosynthetic events in living organisms by isotopic fingerprinting under conditions of imposed isotopic gradients
WO2005094327A2 (en) * 2004-03-29 2005-10-13 The Regents Of The University Of California Isolation of epithelial cells or their biochemical contents from excreta after in vivo isotopic labeling
JP4118918B2 (ja) * 2005-02-28 2008-07-16 シャープ株式会社 信号品質評価装置、情報記録再生装置、信号品質評価方法、記録条件決定方法、信号品質評価プログラム、信号品質評価プログラムを記録したコンピュータ読み取り可能な記録媒体
TW200711660A (en) * 2005-06-10 2007-04-01 Univ California Monitoring two dimensions of diabetes pathogenesis separately or concurrently (insulin sensitivity and beta-cell sufficiency): uses in diagnosis, prognosis, assessment of disease risk, and drug development
JP5448397B2 (ja) 2007-09-07 2014-03-19 キヤノン株式会社 基質プローブ、多重核磁気共鳴法による酵素活性の検出方法および酵素活性のイメージング方法
WO2009105591A2 (en) * 2008-02-19 2009-08-27 The Regents Of The University Of California Methods and systems for genome-scale kinetic modeling
DE102011005600A1 (de) * 2011-03-16 2012-09-20 Siemens Aktiengesellschaft Detektion von Organismen und Bestimmung der Resistenz mit Hilfe der Massenspektrometrie
JP2014526685A (ja) 2011-09-08 2014-10-06 ザ・リージェンツ・オブ・ザ・ユニバーシティ・オブ・カリフォルニア 代謝流量測定、画像化、および顕微鏡法
ES2668678T3 (es) 2011-12-07 2018-05-21 Glaxosmithkline Llc Procedimiento de determinación de la masa muscular esquelética corporal total
US9134319B2 (en) 2013-03-15 2015-09-15 The Regents Of The University Of California Method for replacing biomarkers of protein kinetics from tissue samples by biomarkers of protein kinetics from body fluids after isotopic labeling in vivo
US10607723B2 (en) 2016-07-05 2020-03-31 University Of Kentucky Research Foundation Method and system for identification of metabolites using mass spectra
EP3514797B1 (de) 2018-01-18 2024-04-17 Tata Consultancy Services Limited Verfahren und system zur analyse des metabolischen zustands einer zelle durch messung der konzentrationen von metaboliten
CN110196288A (zh) * 2018-02-26 2019-09-03 中国科学院上海生命科学研究院 一种动态代谢流量分析技术的建立与应用
US10937541B2 (en) 2019-05-28 2021-03-02 PAIGE.AI, Inc. Systems and methods for processing images to prepare slides for processed images for digital pathology
CN111575336A (zh) * 2020-05-14 2020-08-25 华东理工大学 一种用于代谢稳态同位素非稳态下获取胞内中心碳代谢途径代谢通量的方法
CN114577916B (zh) * 2020-12-01 2022-12-09 中国科学院大连化学物理研究所 一种基于芯片纳升电喷雾质谱的稳定同位素标记代谢流的分析方法

Family Cites Families (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6764817B1 (en) * 1999-04-20 2004-07-20 Target Discovery, Inc. Methods for conducting metabolic analyses
EP2293056A3 (de) 1999-04-20 2011-06-29 Target Discovery, Inc. Verfahren zur Analyse eines Stoffwechselweges
EP1381860A4 (de) 2001-03-01 2008-10-15 Univ California Modelle und verfahren zur bestimmung systemischer eigenschaften regulierter reaktionsnetzwerke
EP1546364A4 (de) 2002-07-30 2006-09-06 Univ California Verfahren zur automatischen messung im grossmassstab der molekularen flussgeschwindigkeiten des proteoms oder des organoms mittels massenspektrometrie
WO2005001736A2 (en) 2003-06-30 2005-01-06 Ajinomoto Co., Inc. Intracellular metabolic flux analysis method using substrate labeled with isotope
KR101073370B1 (ko) 2003-07-29 2011-10-17 아지노모토 가부시키가이샤 물질 생산에 영향을 미치는 대사 흐름을 결정하는 방법

Also Published As

Publication number Publication date
US20050175982A1 (en) 2005-08-11
EP1562044A1 (de) 2005-08-10
EP1562044B1 (de) 2010-09-15
DE602005023552D1 (de) 2010-10-28
US8510054B2 (en) 2013-08-13

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