ATE463565T1 - Kontrolierte elektroporation - Google Patents

Kontrolierte elektroporation

Info

Publication number
ATE463565T1
ATE463565T1 AT00948879T AT00948879T ATE463565T1 AT E463565 T1 ATE463565 T1 AT E463565T1 AT 00948879 T AT00948879 T AT 00948879T AT 00948879 T AT00948879 T AT 00948879T AT E463565 T1 ATE463565 T1 AT E463565T1
Authority
AT
Austria
Prior art keywords
cell
electroporation
voltage
opening
solute
Prior art date
Application number
AT00948879T
Other languages
English (en)
Inventor
Boris Rubinsky
Yong Huang
Original Assignee
Univ California
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from US09/358,510 external-priority patent/US6300108B1/en
Application filed by Univ California filed Critical Univ California
Application granted granted Critical
Publication of ATE463565T1 publication Critical patent/ATE463565T1/de

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/483—Physical analysis of biological material
    • G01N33/487—Physical analysis of biological material of liquid biological material
    • G01N33/48707—Physical analysis of biological material of liquid biological material by electrical means
    • G01N33/48728—Investigating individual cells, e.g. by patch clamp, voltage clamp
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M35/00—Means for application of stress for stimulating the growth of microorganisms or the generation of fermentation or metabolic products; Means for electroporation or cell fusion
    • C12M35/02—Electrical or electromagnetic means, e.g. for electroporation or for cell fusion

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Chemical & Material Sciences (AREA)
  • Physics & Mathematics (AREA)
  • Organic Chemistry (AREA)
  • Biotechnology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Genetics & Genomics (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Molecular Biology (AREA)
  • Analytical Chemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Electromagnetism (AREA)
  • Biophysics (AREA)
  • Hematology (AREA)
  • Cell Biology (AREA)
  • Urology & Nephrology (AREA)
  • Food Science & Technology (AREA)
  • Medicinal Chemistry (AREA)
  • Sustainable Development (AREA)
  • General Physics & Mathematics (AREA)
  • Immunology (AREA)
  • Pathology (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Immobilizing And Processing Of Enzymes And Microorganisms (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Selective Calling Equipment (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Electrotherapy Devices (AREA)
AT00948879T 1999-07-21 2000-07-20 Kontrolierte elektroporation ATE463565T1 (de)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US09/358,510 US6300108B1 (en) 1999-07-21 1999-07-21 Controlled electroporation and mass transfer across cell membranes
US09/618,949 US6403348B1 (en) 1999-07-21 2000-07-19 Controlled electroporation and mass transfer across cell membranes
PCT/US2000/019975 WO2001007584A1 (en) 1999-07-21 2000-07-20 Controlled electroporation and mass transfer across cell membranes

Publications (1)

Publication Number Publication Date
ATE463565T1 true ATE463565T1 (de) 2010-04-15

Family

ID=27000090

Family Applications (1)

Application Number Title Priority Date Filing Date
AT00948879T ATE463565T1 (de) 1999-07-21 2000-07-20 Kontrolierte elektroporation

Country Status (8)

Country Link
US (1) US6403348B1 (de)
EP (1) EP1196549B1 (de)
JP (1) JP2003505073A (de)
AT (1) ATE463565T1 (de)
CA (1) CA2378113C (de)
DE (1) DE60044129D1 (de)
ES (1) ES2341753T3 (de)
WO (1) WO2001007584A1 (de)

Families Citing this family (93)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7053063B2 (en) * 1999-07-21 2006-05-30 The Regents Of The University Of California Controlled electroporation and mass transfer across cell membranes in tissue
US6927049B2 (en) * 1999-07-21 2005-08-09 The Regents Of The University Of California Cell viability detection using electrical measurements
US6300108B1 (en) * 1999-07-21 2001-10-09 The Regents Of The University Of California Controlled electroporation and mass transfer across cell membranes
IT1320186B1 (it) * 2000-04-21 2003-11-26 Igea Srl Dispositivo e metodo di elettro-porazione in cui l'ampiezzadell'impulso o degli impulsi viene stabilita in modo automatico in
IT1320187B1 (it) * 2000-04-21 2003-11-26 Igea Srl Dispositivo e metodo di elettro-porazione che fornisce un segnalemodulato sotto il controllo continuo della elettro-permeabilizzazione
US6697670B2 (en) 2001-08-17 2004-02-24 Minnesota Medical Physics, Llc Apparatus and method for reducing subcutaneous fat deposits by electroporation with improved comfort of patients
US6892099B2 (en) 2001-02-08 2005-05-10 Minnesota Medical Physics, Llc Apparatus and method for reducing subcutaneous fat deposits, virtual face lift and body sculpturing by electroporation
US6795728B2 (en) 2001-08-17 2004-09-21 Minnesota Medical Physics, Llc Apparatus and method for reducing subcutaneous fat deposits by electroporation
US8251986B2 (en) 2000-08-17 2012-08-28 Angiodynamics, Inc. Method of destroying tissue cells by eletroporation
US6460413B1 (en) * 2001-03-15 2002-10-08 Gerald J. Diebold Imaging based on the electroacoustic effect
US7130697B2 (en) * 2002-08-13 2006-10-31 Minnesota Medical Physics Llc Apparatus and method for the treatment of benign prostatic hyperplasia
US6994706B2 (en) * 2001-08-13 2006-02-07 Minnesota Medical Physics, Llc Apparatus and method for treatment of benign prostatic hyperplasia
USRE42016E1 (en) 2001-08-13 2010-12-28 Angiodynamics, Inc. Apparatus and method for the treatment of benign prostatic hyperplasia
DE10144486C1 (de) * 2001-09-10 2003-04-24 Karlsruhe Forschzent Verfahren zum kontinuierlichen nichtthermischen Aufschluß und Pasteurisieren industrieller Mengen organischen Prozessguts durch Elektroporation und Reaktor zum Durchführen des Verfahrens
DE10202094B4 (de) 2002-01-21 2006-09-28 Eppendorf Ag Verfahren und Vorrichtung zur Elektroporation biologischer Zellen
ITTO20020477A1 (it) * 2002-06-07 2003-12-09 Igea Srl Dispositivo per elettroporazione.
EP1565555A4 (de) * 2002-09-30 2008-07-09 Maxcyte Inc Vorrichtung und verfahren zur strämungselektroporation
JP2004344003A (ja) * 2003-04-22 2004-12-09 Institute Of Physical & Chemical Research 細胞刺激装置及び細胞刺激方法
US6969604B1 (en) 2003-06-20 2005-11-29 Yakovenko Sergey A Electroporation chamber
US20050118705A1 (en) * 2003-11-07 2005-06-02 Rabbitt Richard D. Electrical detectors for microanalysis
US20050170510A1 (en) * 2003-12-08 2005-08-04 Yong Huang Device and method for controlled electroporation and molecular delivery into cells and tissue
US8298222B2 (en) 2003-12-24 2012-10-30 The Regents Of The University Of California Electroporation to deliver chemotherapeutics and enhance tumor regression
CA2550846C (en) * 2003-12-24 2018-01-23 The Regents Of The University Of California Tissue ablation with irreversible electroporation
US8058056B2 (en) * 2004-03-12 2011-11-15 The Regents Of The University Of California Method and apparatus for integrated cell handling and measurements
JP4443278B2 (ja) * 2004-03-26 2010-03-31 テルモ株式会社 拡張体付カテーテル
JP4188289B2 (ja) * 2004-07-27 2008-11-26 株式会社東芝 体内細胞通電装置
US20080206828A1 (en) * 2005-03-17 2008-08-28 Kyoto University Device For Introducing Substance Into Cell, Cell Clamping Device and Flow Path Forming Method
US20060264752A1 (en) * 2005-04-27 2006-11-23 The Regents Of The University Of California Electroporation controlled with real time imaging
US20060293731A1 (en) * 2005-06-24 2006-12-28 Boris Rubinsky Methods and systems for treating tumors using electroporation
US8114070B2 (en) 2005-06-24 2012-02-14 Angiodynamics, Inc. Methods and systems for treating BPH using electroporation
US20060293725A1 (en) * 2005-06-24 2006-12-28 Boris Rubinsky Methods and systems for treating fatty tissue sites using electroporation
US20060293730A1 (en) 2005-06-24 2006-12-28 Boris Rubinsky Methods and systems for treating restenosis sites using electroporation
US8214221B2 (en) * 2005-06-30 2012-07-03 Lg Electronics Inc. Method and apparatus for decoding an audio signal and identifying information included in the audio signal
US20070156135A1 (en) * 2006-01-03 2007-07-05 Boris Rubinsky System and methods for treating atrial fibrillation using electroporation
US8293524B2 (en) * 2006-03-31 2012-10-23 Fluxion Biosciences Inc. Methods and apparatus for the manipulation of particle suspensions and testing thereof
US7687267B2 (en) * 2006-09-30 2010-03-30 Rational Biotechnology Inc. High-throughput cell transfection device and methods of using thereof
JP2010506657A (ja) 2006-10-16 2010-03-04 ザ リージェンツ オブ ザ ユニバーシティ オブ カリフォルニア 組織の不可逆的エレクトロポレーションに使用される、所定の導電率を有するゲル
US20080132884A1 (en) * 2006-12-01 2008-06-05 Boris Rubinsky Systems for treating tissue sites using electroporation
DE102007016927A1 (de) 2007-04-05 2008-10-16 Kern, Martin Verfahren zur reversiblen und irreversiblen elektrophysikalischen Zellporation und die zur Durchführung erforderlichen Porationsreaktoren
US20090137925A1 (en) * 2007-11-23 2009-05-28 Divya Cantor Impedance Spectroscopy Cervix Scanning Apparatus and Method
US20100004623A1 (en) * 2008-03-27 2010-01-07 Angiodynamics, Inc. Method for Treatment of Complications Associated with Arteriovenous Grafts and Fistulas Using Electroporation
WO2009121017A1 (en) 2008-03-27 2009-10-01 The Regents Of The University Of California Balloon catheter for reducing restenosis via irreversible electroporation
CA2722296A1 (en) * 2008-04-29 2009-11-05 Virginia Tech Intellectual Properties, Inc. Irreversible electroporation to create tissue scaffolds
US11272979B2 (en) 2008-04-29 2022-03-15 Virginia Tech Intellectual Properties, Inc. System and method for estimating tissue heating of a target ablation zone for electrical-energy based therapies
US10238447B2 (en) 2008-04-29 2019-03-26 Virginia Tech Intellectual Properties, Inc. System and method for ablating a tissue site by electroporation with real-time monitoring of treatment progress
US10272178B2 (en) 2008-04-29 2019-04-30 Virginia Tech Intellectual Properties Inc. Methods for blood-brain barrier disruption using electrical energy
US9198733B2 (en) 2008-04-29 2015-12-01 Virginia Tech Intellectual Properties, Inc. Treatment planning for electroporation-based therapies
US10117707B2 (en) 2008-04-29 2018-11-06 Virginia Tech Intellectual Properties, Inc. System and method for estimating tissue heating of a target ablation zone for electrical-energy based therapies
US9283051B2 (en) 2008-04-29 2016-03-15 Virginia Tech Intellectual Properties, Inc. System and method for estimating a treatment volume for administering electrical-energy based therapies
US10702326B2 (en) 2011-07-15 2020-07-07 Virginia Tech Intellectual Properties, Inc. Device and method for electroporation based treatment of stenosis of a tubular body part
US11254926B2 (en) 2008-04-29 2022-02-22 Virginia Tech Intellectual Properties, Inc. Devices and methods for high frequency electroporation
US10245098B2 (en) 2008-04-29 2019-04-02 Virginia Tech Intellectual Properties, Inc. Acute blood-brain barrier disruption using electrical energy based therapy
US9867652B2 (en) 2008-04-29 2018-01-16 Virginia Tech Intellectual Properties, Inc. Irreversible electroporation using tissue vasculature to treat aberrant cell masses or create tissue scaffolds
US8926606B2 (en) * 2009-04-09 2015-01-06 Virginia Tech Intellectual Properties, Inc. Integration of very short electric pulses for minimally to noninvasive electroporation
US8992517B2 (en) * 2008-04-29 2015-03-31 Virginia Tech Intellectual Properties Inc. Irreversible electroporation to treat aberrant cell masses
WO2009137800A2 (en) 2008-05-09 2009-11-12 Angiodynamics, Inc. Electroporation device and method
US9173704B2 (en) * 2008-06-20 2015-11-03 Angiodynamics, Inc. Device and method for the ablation of fibrin sheath formation on a venous catheter
WO2010008834A2 (en) * 2008-06-23 2010-01-21 Angiodynamics, Inc. Treatment devices and methods
US20100152725A1 (en) * 2008-12-12 2010-06-17 Angiodynamics, Inc. Method and system for tissue treatment utilizing irreversible electroporation and thermal track coagulation
WO2010085765A2 (en) * 2009-01-23 2010-07-29 Moshe Meir H Therapeutic energy delivery device with rotational mechanism
WO2010093692A2 (en) * 2009-02-10 2010-08-19 Hobbs Eamonn P Irreversible electroporation and tissue regeneration
US11382681B2 (en) 2009-04-09 2022-07-12 Virginia Tech Intellectual Properties, Inc. Device and methods for delivery of high frequency electrical pulses for non-thermal ablation
US11638603B2 (en) 2009-04-09 2023-05-02 Virginia Tech Intellectual Properties, Inc. Selective modulation of intracellular effects of cells using pulsed electric fields
USD630321S1 (en) 2009-05-08 2011-01-04 Angio Dynamics, Inc. Probe handle
US8903488B2 (en) 2009-05-28 2014-12-02 Angiodynamics, Inc. System and method for synchronizing energy delivery to the cardiac rhythm
US9895189B2 (en) 2009-06-19 2018-02-20 Angiodynamics, Inc. Methods of sterilization and treating infection using irreversible electroporation
EP2488251A4 (de) * 2009-10-16 2014-02-19 Virginia Tech Intell Prop Behandlungsplanung für auf elektroporation beruhende therapien
US20110118732A1 (en) 2009-11-19 2011-05-19 The Regents Of The University Of California Controlled irreversible electroporation
EP2627274B1 (de) 2010-10-13 2022-12-14 AngioDynamics, Inc. System zur elektrischen ableitung des gewebes eines patienten
WO2012088149A2 (en) 2010-12-20 2012-06-28 Virginia Tech Intellectual Properties, Inc. High-frequency electroporation for cancer therapy
US9266725B2 (en) 2011-04-27 2016-02-23 The Board Of Trustees Of The Leland Stanford Junior University Nanotube structures, methods of making nanotube structures, and methods of accessing intracellular space
US9078665B2 (en) 2011-09-28 2015-07-14 Angiodynamics, Inc. Multiple treatment zone ablation probe
US9414881B2 (en) 2012-02-08 2016-08-16 Angiodynamics, Inc. System and method for increasing a target zone for electrical ablation
EP4000685A1 (de) 2013-11-14 2022-05-25 RM2 Technology LLC Systeme und vorrichtungen zur gewebeablation unter verwendung von elektrolyse und permeabilisierung
US10166321B2 (en) 2014-01-09 2019-01-01 Angiodynamics, Inc. High-flow port and infusion needle systems
EP3143124A4 (de) 2014-05-12 2018-01-17 Virginia Tech Intellectual Properties, Inc. Selektive modulation intrazellulärer effekte von zellen mittels gepulster elektrischer felder
US10694972B2 (en) 2014-12-15 2020-06-30 Virginia Tech Intellectual Properties, Inc. Devices, systems, and methods for real-time monitoring of electrophysical effects during tissue treatment
US10167502B2 (en) 2015-04-03 2019-01-01 Fluxion Biosciences, Inc. Molecular characterization of single cells and cell populations for non-invasive diagnostics
CN112998846A (zh) 2015-05-01 2021-06-22 因特科学股份有限公司 用于使用脉冲形状设计的组织消融的方法、系统及设备
JP6989133B2 (ja) * 2015-09-04 2022-01-05 ラトガース ザ ステイト ユニバーシティー オブ ニュージャージー 高スループット、単一細胞への効率的な分子送達のためのフィードバック制御されたエレクトロポレーションマイクロデバイス
KR20190019129A (ko) * 2016-06-09 2019-02-26 더 보드 어브 트러스티스 어브 더 리랜드 스탠포드 주니어 유니버시티 개선된 세포 트랜스펙션 및 생존율을 위한 나노스트로 웰 인서트 디바이스
US11149266B2 (en) 2016-09-13 2021-10-19 The Board Of Trustees Of The Leland Stanford Junior University Methods of non-destructive nanostraw intracellular sampling for longitudinal cell monitoring
US10905492B2 (en) 2016-11-17 2021-02-02 Angiodynamics, Inc. Techniques for irreversible electroporation using a single-pole tine-style internal device communicating with an external surface electrode
WO2018144814A1 (en) 2017-02-03 2018-08-09 Infinitesimal Llc Electroporation system with micromanipulator and probe
US11607537B2 (en) 2017-12-05 2023-03-21 Virginia Tech Intellectual Properties, Inc. Method for treating neurological disorders, including tumors, with electroporation
US11925405B2 (en) 2018-03-13 2024-03-12 Virginia Tech Intellectual Properties, Inc. Treatment planning system for immunotherapy enhancement via non-thermal ablation
US11311329B2 (en) 2018-03-13 2022-04-26 Virginia Tech Intellectual Properties, Inc. Treatment planning for immunotherapy based treatments using non-thermal ablation techniques
US11300558B2 (en) * 2018-06-14 2022-04-12 Nokomis, Inc. Apparatus and system for spectroscopy and tomography of fragile biologic materials
JP2019093146A (ja) * 2018-12-13 2019-06-20 パルティ、ヨーラム 交番電界によって腫瘍を治療し、推定される細胞サイズに基づいて治療周波数を選択するための装置および方法
US11950835B2 (en) 2019-06-28 2024-04-09 Virginia Tech Intellectual Properties, Inc. Cycled pulsing to mitigate thermal damage for multi-electrode irreversible electroporation therapy
US20220184610A1 (en) * 2019-07-25 2022-06-16 Hewlett-Packard Development Company, L.P. Cell poration and transfection apparatuses
WO2021015778A1 (en) * 2019-07-25 2021-01-28 Hewlett-Packard Development Company, L.P. Cell poration and transfection apparatuses
US20230174908A1 (en) * 2020-04-30 2023-06-08 Hewlett-Packard Development Company, L.P. Reagent injections into cells

Family Cites Families (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4946793A (en) * 1986-05-09 1990-08-07 Electropore, Inc. Impedance matching for instrumentation which electrically alters vesicle membranes
US5098843A (en) 1987-06-04 1992-03-24 Calvin Noel M Apparatus for the high efficiency transformation of living cells
US5134070A (en) 1990-06-04 1992-07-28 Casnig Dael R Method and device for cell cultivation on electrodes
US5173158A (en) 1991-07-22 1992-12-22 Schmukler Robert E Apparatus and methods for electroporation and electrofusion
ATE258980T1 (de) * 1997-06-10 2004-02-15 Cyto Pulse Sciences Inc Verfahren und apparatur zur behandlung von material mit elektrischen feldern unterschiedlicher orientierung

Also Published As

Publication number Publication date
DE60044129D1 (de) 2010-05-20
WO2001007584A1 (en) 2001-02-01
JP2003505073A (ja) 2003-02-12
CA2378113C (en) 2010-11-23
EP1196549A4 (de) 2006-06-07
EP1196549A1 (de) 2002-04-17
US6403348B1 (en) 2002-06-11
ES2341753T3 (es) 2010-06-28
WO2001007584A8 (en) 2001-07-26
CA2378113A1 (en) 2001-02-01
EP1196549B1 (de) 2010-04-07

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