ATE463565T1 - Kontrolierte elektroporation - Google Patents

Kontrolierte elektroporation

Info

Publication number
ATE463565T1
ATE463565T1 AT00948879T AT00948879T ATE463565T1 AT E463565 T1 ATE463565 T1 AT E463565T1 AT 00948879 T AT00948879 T AT 00948879T AT 00948879 T AT00948879 T AT 00948879T AT E463565 T1 ATE463565 T1 AT E463565T1
Authority
AT
Austria
Prior art keywords
cell
electroporation
voltage
opening
solute
Prior art date
Application number
AT00948879T
Other languages
English (en)
Inventor
Boris Rubinsky
Yong Huang
Original Assignee
Univ California
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from US09/358,510 external-priority patent/US6300108B1/en
Application filed by Univ California filed Critical Univ California
Application granted granted Critical
Publication of ATE463565T1 publication Critical patent/ATE463565T1/de

Links

Classifications

    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/483Physical analysis of biological material
    • G01N33/487Physical analysis of biological material of liquid biological material
    • G01N33/48707Physical analysis of biological material of liquid biological material by electrical means
    • G01N33/48728Investigating individual cells, e.g. by patch clamp, voltage clamp
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12MAPPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M35/00Means for application of stress for stimulating the growth of microorganisms or the generation of fermentation or metabolic products; Means for electroporation or cell fusion
    • C12M35/02Electrical or electromagnetic means, e.g. for electroporation or for cell fusion

Landscapes

  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Biomedical Technology (AREA)
  • Physics & Mathematics (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Organic Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • Biotechnology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Electromagnetism (AREA)
  • Molecular Biology (AREA)
  • Immunology (AREA)
  • Pathology (AREA)
  • Analytical Chemistry (AREA)
  • Medicinal Chemistry (AREA)
  • Cell Biology (AREA)
  • Food Science & Technology (AREA)
  • Urology & Nephrology (AREA)
  • Microbiology (AREA)
  • Sustainable Development (AREA)
  • General Physics & Mathematics (AREA)
  • Hematology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biophysics (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Immobilizing And Processing Of Enzymes And Microorganisms (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Electrotherapy Devices (AREA)
  • Selective Calling Equipment (AREA)
AT00948879T 1999-07-21 2000-07-20 Kontrolierte elektroporation ATE463565T1 (de)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US09/358,510 US6300108B1 (en) 1999-07-21 1999-07-21 Controlled electroporation and mass transfer across cell membranes
US09/618,949 US6403348B1 (en) 1999-07-21 2000-07-19 Controlled electroporation and mass transfer across cell membranes
PCT/US2000/019975 WO2001007584A1 (en) 1999-07-21 2000-07-20 Controlled electroporation and mass transfer across cell membranes

Publications (1)

Publication Number Publication Date
ATE463565T1 true ATE463565T1 (de) 2010-04-15

Family

ID=27000090

Family Applications (1)

Application Number Title Priority Date Filing Date
AT00948879T ATE463565T1 (de) 1999-07-21 2000-07-20 Kontrolierte elektroporation

Country Status (8)

Country Link
US (1) US6403348B1 (de)
EP (1) EP1196549B1 (de)
JP (1) JP2003505073A (de)
AT (1) ATE463565T1 (de)
CA (1) CA2378113C (de)
DE (1) DE60044129D1 (de)
ES (1) ES2341753T3 (de)
WO (1) WO2001007584A1 (de)

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US6300108B1 (en) * 1999-07-21 2001-10-09 The Regents Of The University Of California Controlled electroporation and mass transfer across cell membranes
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Also Published As

Publication number Publication date
EP1196549A4 (de) 2006-06-07
WO2001007584A1 (en) 2001-02-01
ES2341753T3 (es) 2010-06-28
CA2378113C (en) 2010-11-23
JP2003505073A (ja) 2003-02-12
WO2001007584A8 (en) 2001-07-26
CA2378113A1 (en) 2001-02-01
DE60044129D1 (de) 2010-05-20
EP1196549B1 (de) 2010-04-07
EP1196549A1 (de) 2002-04-17
US6403348B1 (en) 2002-06-11

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