ATE461454T1 - Verfahren zur bestimmung von s-nitrosothiolen in biologischen flüssigkeiten - Google Patents

Verfahren zur bestimmung von s-nitrosothiolen in biologischen flüssigkeiten

Info

Publication number
ATE461454T1
ATE461454T1 AT07789530T AT07789530T ATE461454T1 AT E461454 T1 ATE461454 T1 AT E461454T1 AT 07789530 T AT07789530 T AT 07789530T AT 07789530 T AT07789530 T AT 07789530T AT E461454 T1 ATE461454 T1 AT E461454T1
Authority
AT
Austria
Prior art keywords
amount
enzyme
giycysno
gsno
nitrosoglutathione
Prior art date
Application number
AT07789530T
Other languages
English (en)
Inventor
Alfonso Pompella
Aldo Paolicchi
Emilia Bramanti
Renata Barsacchi
Maria Franzini
Cecilia Vecoli
Original Assignee
Univ Pisa
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Univ Pisa filed Critical Univ Pisa
Application granted granted Critical
Publication of ATE461454T1 publication Critical patent/ATE461454T1/de

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/34—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving hydrolase
    • C12Q1/37—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving hydrolase involving peptidase or proteinase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/48—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving transferase
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
    • G01N33/6803—General methods of protein analysis not limited to specific proteins or families of proteins
    • G01N33/6806—Determination of free amino acids
    • G01N33/6812—Assays for specific amino acids
    • G01N33/6815—Assays for specific amino acids containing sulfur, e.g. cysteine, cystine, methionine, homocysteine
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
    • G01N33/6803—General methods of protein analysis not limited to specific proteins or families of proteins
    • G01N33/6842—Proteomic analysis of subsets of protein mixtures with reduced complexity, e.g. membrane proteins, phosphoproteins, organelle proteins
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/94—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving narcotics or drugs or pharmaceuticals, neurotransmitters or associated receptors
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2333/00—Assays involving biological materials from specific organisms or of a specific nature
    • G01N2333/90—Enzymes; Proenzymes
    • G01N2333/91—Transferases (2.)
    • G01N2333/91045—Acyltransferases (2.3)
    • G01N2333/91074—Aminoacyltransferases (general) (2.3.2)
    • G01N2333/9108—Aminoacyltransferases (general) (2.3.2) with definite EC number (2.3.2.-)
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2333/00—Assays involving biological materials from specific organisms or of a specific nature
    • G01N2333/90—Enzymes; Proenzymes
    • G01N2333/914—Hydrolases (3)
    • G01N2333/948—Hydrolases (3) acting on peptide bonds (3.4)
    • G01N2333/95—Proteinases, i.e. endopeptidases (3.4.21-3.4.99)
    • G01N2333/964—Proteinases, i.e. endopeptidases (3.4.21-3.4.99) derived from animal tissue
    • G01N2333/96425—Proteinases, i.e. endopeptidases (3.4.21-3.4.99) derived from animal tissue from mammals
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2400/00—Assays, e.g. immunoassays or enzyme assays, involving carbohydrates

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Molecular Biology (AREA)
  • Immunology (AREA)
  • Physics & Mathematics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Urology & Nephrology (AREA)
  • Biomedical Technology (AREA)
  • Organic Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Hematology (AREA)
  • Biochemistry (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biophysics (AREA)
  • Analytical Chemistry (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Medicinal Chemistry (AREA)
  • Food Science & Technology (AREA)
  • Cell Biology (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • General Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Organic Low-Molecular-Weight Compounds And Preparation Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Investigating Or Analysing Materials By The Use Of Chemical Reactions (AREA)
AT07789530T 2006-07-21 2007-07-23 Verfahren zur bestimmung von s-nitrosothiolen in biologischen flüssigkeiten ATE461454T1 (de)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
IT000093A ITPI20060093A1 (it) 2006-07-21 2006-07-21 Procedimento per la determinazione di s-nitrosotioli in fluidi biologici
PCT/IB2007/002090 WO2008012642A2 (en) 2006-07-21 2007-07-23 Process for determining s-nitrosothiols in biological fluids

Publications (1)

Publication Number Publication Date
ATE461454T1 true ATE461454T1 (de) 2010-04-15

Family

ID=38981837

Family Applications (1)

Application Number Title Priority Date Filing Date
AT07789530T ATE461454T1 (de) 2006-07-21 2007-07-23 Verfahren zur bestimmung von s-nitrosothiolen in biologischen flüssigkeiten

Country Status (6)

Country Link
US (1) US8148099B2 (de)
EP (1) EP2054727B1 (de)
AT (1) ATE461454T1 (de)
DE (1) DE602007005381D1 (de)
IT (1) ITPI20060093A1 (de)
WO (1) WO2008012642A2 (de)

Families Citing this family (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2010141719A2 (en) * 2009-06-03 2010-12-09 University Of Florida Research Foundation Inc. Materials and methods for measuring nitric oxide levels in a biological fluid
CN110221006A (zh) * 2019-07-25 2019-09-10 深圳健科医学检验实验室 一种针对巯基多肽生物样本的前处理方法以及对该生物样本的检测方法
CN112945948A (zh) * 2021-02-03 2021-06-11 迪瑞医疗科技股份有限公司 含有氮氧自由基类化合物作为稳定剂在制备尿潜血试纸中的应用
US20230050352A1 (en) * 2021-08-13 2023-02-16 University Hospitals Cleveland Medical Center Non-invasive measurement of endogenous s-nitrosothiols
CN115236214A (zh) * 2021-11-12 2022-10-25 上海旭东海普药业有限公司 一种乙酰半胱氨酸溶液中EDTA-2Na的检测方法

Family Cites Families (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
AU2002214668A1 (en) * 2000-10-27 2002-05-21 Memorial Sloan-Kettering Cancer Center Method for assaying protein nitrosylation
ITTO20070455A1 (it) * 2007-06-25 2008-12-26 Univ Pisa Procedimento di determinazione e di separazione di isoforme di gamma-glutamiltransferasi (ggt) sierica in un campione di fluido biologico e isoforme enzimatiche cosi' ottenute

Also Published As

Publication number Publication date
US8148099B2 (en) 2012-04-03
WO2008012642A3 (en) 2008-08-28
WO2008012642A2 (en) 2008-01-31
EP2054727B1 (de) 2010-03-17
EP2054727A2 (de) 2009-05-06
ITPI20060093A1 (it) 2008-01-22
US20090275012A1 (en) 2009-11-05
DE602007005381D1 (de) 2010-04-29

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