ATE453710T1 - Methode zur diversitätsbildung - Google Patents

Methode zur diversitätsbildung

Info

Publication number
ATE453710T1
ATE453710T1 AT99947775T AT99947775T ATE453710T1 AT E453710 T1 ATE453710 T1 AT E453710T1 AT 99947775 T AT99947775 T AT 99947775T AT 99947775 T AT99947775 T AT 99947775T AT E453710 T1 ATE453710 T1 AT E453710T1
Authority
AT
Austria
Prior art keywords
diversity
gene
genes
rate
education
Prior art date
Application number
AT99947775T
Other languages
English (en)
Inventor
Julian Edward Sale
Michael S Neuberger
Sarah Jane Cumbers
Original Assignee
Medical Res Council
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from GBGB9822104.7A external-priority patent/GB9822104D0/en
Priority claimed from GBGB9901141.3A external-priority patent/GB9901141D0/en
Priority claimed from GBGB9913435.5A external-priority patent/GB9913435D0/en
Application filed by Medical Res Council filed Critical Medical Res Council
Application granted granted Critical
Publication of ATE453710T1 publication Critical patent/ATE453710T1/de

Links

Classifications

    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01K—ANIMAL HUSBANDRY; CARE OF BIRDS, FISHES, INSECTS; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K67/00—Rearing or breeding animals, not otherwise provided for; New breeds of animals
    • A01K67/027—New breeds of vertebrates
    • A01K67/0275—Genetically modified vertebrates, e.g. transgenic
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/01—Preparation of mutants without inserting foreign genetic material therein; Screening processes therefor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/102—Mutagenizing nucleic acids
    • C12N15/1024—In vivo mutagenesis using high mutation rate "mutator" host strains by inserting genetic material, e.g. encoding an error prone polymerase, disrupting a gene for mismatch repair
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C12N15/1075—Isolating an individual clone by screening libraries by coupling phenotype to genotype, not provided for in other groups of this subclass
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01K—ANIMAL HUSBANDRY; CARE OF BIRDS, FISHES, INSECTS; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K2217/00—Genetically modified animals
    • A01K2217/05—Animals comprising random inserted nucleic acids (transgenic)
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2317/00—Immunoglobulins specific features
    • C07K2317/50—Immunoglobulins specific features characterized by immunoglobulin fragments
    • C07K2317/56—Immunoglobulins specific features characterized by immunoglobulin fragments variable (Fv) region, i.e. VH and/or VL
AT99947775T 1998-10-09 1999-10-08 Methode zur diversitätsbildung ATE453710T1 (de)

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
GBGB9822104.7A GB9822104D0 (en) 1998-10-09 1998-10-09 Patent application
GBGB9901141.3A GB9901141D0 (en) 1999-01-19 1999-01-19 Method for generating diversity
GBGB9913435.5A GB9913435D0 (en) 1999-06-09 1999-06-09 Method for generating diversity
PCT/GB1999/003358 WO2000022111A1 (en) 1998-10-09 1999-10-08 Method for generating diversity

Publications (1)

Publication Number Publication Date
ATE453710T1 true ATE453710T1 (de) 2010-01-15

Family

ID=27269511

Family Applications (1)

Application Number Title Priority Date Filing Date
AT99947775T ATE453710T1 (de) 1998-10-09 1999-10-08 Methode zur diversitätsbildung

Country Status (12)

Country Link
US (1) US20030087236A1 (de)
EP (2) EP2204447B1 (de)
JP (1) JP4302894B2 (de)
AT (1) ATE453710T1 (de)
AU (1) AU776049B2 (de)
CA (2) CA2675802A1 (de)
CY (1) CY1109986T1 (de)
DE (1) DE69941876D1 (de)
DK (2) DK1117774T3 (de)
ES (2) ES2336982T3 (de)
PT (2) PT2204447T (de)
WO (1) WO2000022111A1 (de)

Families Citing this family (14)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7122339B2 (en) * 1998-10-09 2006-10-17 Medical Research Council Method for generating diversity
FR2833612B1 (fr) * 2001-12-18 2006-01-27 Inst Necker Procede pour l'induction maitrisee de mutations somatiques et son application en proteomique
FR2833611B1 (fr) * 2001-12-18 2004-04-02 Inst Necker Procede pour l'induction de mutations somatiques et son application en proteomique
EP1536004B1 (de) * 2002-07-30 2010-12-01 Riken Verfahren zur förderung der homologen rekombination somatischer zellen und verfahren zur konstruktion eines spezifischen antikörpers
ATE483822T1 (de) * 2002-10-11 2010-10-15 Univ Erasmus Primer für nukleinsäureamplifikation in pcr- basierten klonalitätsstudien
AU2003287780B2 (en) * 2002-12-18 2007-03-22 Anaptysbio, Inc. In vivo affinity maturation scheme
AU2002953381A0 (en) * 2002-12-18 2003-01-09 Diatech Pty Ltd In vivo affinity maturation scheme
EP1568765A1 (de) * 2004-02-23 2005-08-31 GSF-Forschungszentrum für Umwelt und Gesundheit GmbH Methode zur genetischen Diversifikation in Zellen, in denen Genkonversion auftritt
US7393923B2 (en) * 2004-11-08 2008-07-01 The Regents Of The University Of California Red-shifted fluorescent proteins mPlum and mRaspberry and polynucleotides encoding the same
US8603950B2 (en) 2007-02-20 2013-12-10 Anaptysbio, Inc. Methods of generating libraries and uses thereof
EP2152861B1 (de) 2007-05-31 2017-03-22 University of Washington Herbeiführbare mutagenese von target-genen
AR068767A1 (es) 2007-10-12 2009-12-02 Novartis Ag Anticuerpos contra esclerostina, composiciones y metodos de uso de estos anticuerpos para tratar un trastorno patologico mediado por esclerostina
EP2098536A1 (de) 2008-03-05 2009-09-09 4-Antibody AG Isolation und Identifikation antigen- oder ligandenspezifischer Bindungsproteine
GB201223053D0 (en) 2012-12-20 2013-02-06 Medical Res Council Receptor

Family Cites Families (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
FR2685004A1 (fr) * 1991-12-11 1993-06-18 Pasteur Institut Procede de generation d'une diversite structurale et fonctionnelle dans une sequence peptidique.
WO1993022457A1 (en) * 1992-04-24 1993-11-11 Massachusetts Institute Of Technology Screening for genetic variation
US6027877A (en) * 1993-11-04 2000-02-22 Gene Check, Inc. Use of immobilized mismatch binding protein for detection of mutations and polymorphisms, purification of amplified DNA samples and allele identification

Also Published As

Publication number Publication date
CA2346927A1 (en) 2000-04-20
EP2204447A3 (de) 2011-05-25
US20030087236A1 (en) 2003-05-08
DK1117774T3 (da) 2010-05-03
JP4302894B2 (ja) 2009-07-29
AU6114299A (en) 2000-05-01
CA2346927C (en) 2009-09-22
PT2204447T (pt) 2016-08-31
CY1109986T1 (el) 2014-09-10
EP2204447A2 (de) 2010-07-07
DE69941876D1 (de) 2010-02-11
EP1117774A1 (de) 2001-07-25
EP1117774B1 (de) 2009-12-30
ES2336982T3 (es) 2010-04-19
PT1117774E (pt) 2010-02-17
JP2002527060A (ja) 2002-08-27
ES2588910T3 (es) 2016-11-07
WO2000022111A1 (en) 2000-04-20
AU776049B2 (en) 2004-08-26
CA2675802A1 (en) 2000-04-20
DK2204447T3 (en) 2016-09-12
EP2204447B1 (de) 2016-06-01

Similar Documents

Publication Publication Date Title
CY1109986T1 (el) Μεθοδος δημιουργιας ποικιλοτητας
Okada et al. CRISPR/Cas9‐mediated knockout of Ms1 enables the rapid generation of male‐sterile hexaploid wheat lines for use in hybrid seed production
Kobayashi et al. Contribution of intragenic DNA methylation in mouse gametic DNA methylomes to establish oocyte-specific heritable marks
GB2349885A (en) Characterisation of gene function using double stranded RNA inhibition
STAM et al. Microsatellite loci in eelgrass Zostera marina reveal marked polymorphism within and among populations
DE69739192D1 (de) Indexierte zell-bibliothek mit genomischen veränderungen und verfahren zur deren herstellung und verwendung
ATE317440T1 (de) Neuartige methode zur integration von fremd-dna ins eukaryotische genom
WO2001070816A3 (en) Ecdysone receptor-based inducible gene expression system
WO2002100998A3 (en) Method for generating diversity
ATE296356T1 (de) Methode zur spezifischen integration von genen in säugetierzellen durch homologe rekombination, und vektoren zu deren durchführung
AU3365095A (en) High throughput screening method for sequences or genetic alterations in nucleic acids
WO2002066613A3 (en) Novel ecdysone receptor/invertebrate retinoid x receptor-based inducible gene expression system
Kim et al. Accessing epigenetic variation in the plant methylome
Brosh et al. Synthetic regulatory genomics uncovers enhancer context dependence at the Sox2 locus
Matsuzaki et al. Recapitulation of gametic DNA methylation and its post-fertilization maintenance with reassembled DNA elements at the mouse Igf2/H19 locus
ATE412760T1 (de) Verfahren zur einführung von inhibitorischer rna in pflanzen und deren anwendungen
WO2003018765A3 (en) A high throughput method for identification of sequence tags
AU5728296A (en) Method to diagnose hereditary hemochromatosis
Verbeelen et al. Optimization of RNA extraction for bacterial whole transcriptome studies of low-biomass samples
CN109628447B (zh) 特异靶向羊友好位点H11的sgRNA及其编码DNA和应用
Jørgensen et al. Design and direct assembly of synthesized uracil-containing non-clonal DNA fragments into vectors by USERTM cloning
WO2001042481A3 (en) Canine adenovirus vectors for the transfer of genes into target cells
Johannesen et al. Characterisation of microsatellite and SNP markers from Miseq and genotyping-by-sequencing data among parapatric Urophora cardui (Tephritidae) populations
WO2001046468A3 (en) Screening method for ros-induced apoptosis in a cell
WO2003040324A3 (en) Methods for mutating genes in cells and animals using insertional mutagenesis

Legal Events

Date Code Title Description
UEP Publication of translation of european patent specification

Ref document number: 1117774

Country of ref document: EP