ATE449845T1 - Verfahren zur modulation der entwicklung eines durch eine nukleinsäuresequenz kodierten polypeptids - Google Patents

Verfahren zur modulation der entwicklung eines durch eine nukleinsäuresequenz kodierten polypeptids

Info

Publication number
ATE449845T1
ATE449845T1 AT05850658T AT05850658T ATE449845T1 AT E449845 T1 ATE449845 T1 AT E449845T1 AT 05850658 T AT05850658 T AT 05850658T AT 05850658 T AT05850658 T AT 05850658T AT E449845 T1 ATE449845 T1 AT E449845T1
Authority
AT
Austria
Prior art keywords
modulating
development
nucleic acid
acid sequence
polypeptide encoded
Prior art date
Application number
AT05850658T
Other languages
English (en)
Inventor
Didier Mazel
Guillaume Cambray
Original Assignee
Pasteur Institut
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Pasteur Institut filed Critical Pasteur Institut
Application granted granted Critical
Publication of ATE449845T1 publication Critical patent/ATE449845T1/de

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/0012—Oxidoreductases (1.) acting on nitrogen containing compounds as donors (1.4, 1.5, 1.6, 1.7)
    • C12N9/0026—Oxidoreductases (1.) acting on nitrogen containing compounds as donors (1.4, 1.5, 1.6, 1.7) acting on CH-NH groups of donors (1.5)
    • C12N9/0028—Oxidoreductases (1.) acting on nitrogen containing compounds as donors (1.4, 1.5, 1.6, 1.7) acting on CH-NH groups of donors (1.5) with NAD or NADP as acceptor (1.5.1)
    • C12N9/003—Dihydrofolate reductase [DHFR] (1.5.1.3)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/01—Preparation of mutants without inserting foreign genetic material therein; Screening processes therefor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/102—Mutagenizing nucleic acids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C12N15/1058—Directional evolution of libraries, e.g. evolution of libraries is achieved by mutagenesis and screening or selection of mixed population of organisms
    • C—CHEMISTRY; METALLURGY
    • C40—COMBINATORIAL TECHNOLOGY
    • C40B—COMBINATORIAL CHEMISTRY; LIBRARIES, e.g. CHEMICAL LIBRARIES
    • C40B10/00—Directed molecular evolution of macromolecules, e.g. RNA, DNA or proteins
    • G—PHYSICS
    • G16—INFORMATION AND COMMUNICATION TECHNOLOGY [ICT] SPECIALLY ADAPTED FOR SPECIFIC APPLICATION FIELDS
    • G16B—BIOINFORMATICS, i.e. INFORMATION AND COMMUNICATION TECHNOLOGY [ICT] SPECIALLY ADAPTED FOR GENETIC OR PROTEIN-RELATED DATA PROCESSING IN COMPUTATIONAL MOLECULAR BIOLOGY
    • G16B30/00—ICT specially adapted for sequence analysis involving nucleotides or amino acids
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10T—TECHNICAL SUBJECTS COVERED BY FORMER US CLASSIFICATION
    • Y10T436/00—Chemistry: analytical and immunological testing
    • Y10T436/14—Heterocyclic carbon compound [i.e., O, S, N, Se, Te, as only ring hetero atom]
    • Y10T436/142222—Hetero-O [e.g., ascorbic acid, etc.]
    • Y10T436/143333—Saccharide [e.g., DNA, etc.]

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Organic Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • General Health & Medical Sciences (AREA)
  • Molecular Biology (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Medicinal Chemistry (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Evolutionary Biology (AREA)
  • Theoretical Computer Science (AREA)
  • Spectroscopy & Molecular Physics (AREA)
  • Analytical Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Medical Informatics (AREA)
  • Ecology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Peptides Or Proteins (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
AT05850658T 2004-09-17 2005-09-19 Verfahren zur modulation der entwicklung eines durch eine nukleinsäuresequenz kodierten polypeptids ATE449845T1 (de)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US61059704P 2004-09-17 2004-09-17
PCT/IB2005/003317 WO2006046132A2 (en) 2004-09-17 2005-09-19 Method for modulating the evolution of a polypeptide encoded by a nucleic acid sequence

Publications (1)

Publication Number Publication Date
ATE449845T1 true ATE449845T1 (de) 2009-12-15

Family

ID=36228153

Family Applications (1)

Application Number Title Priority Date Filing Date
AT05850658T ATE449845T1 (de) 2004-09-17 2005-09-19 Verfahren zur modulation der entwicklung eines durch eine nukleinsäuresequenz kodierten polypeptids

Country Status (5)

Country Link
US (2) US20060177839A1 (de)
EP (1) EP1799823B9 (de)
AT (1) ATE449845T1 (de)
DE (1) DE602005017917D1 (de)
WO (1) WO2006046132A2 (de)

Families Citing this family (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20060177839A1 (en) * 2004-09-17 2006-08-10 Didier Mazel Method for modulating the evolution of a polypeptide encoded by a nucleic acid sequence
US20180010136A1 (en) * 2014-05-30 2018-01-11 John Francis Hunt, III Methods for Altering Polypeptide Expression
CN110305880A (zh) * 2019-06-06 2019-10-08 安子琛 一种基于密码子同义突变的基因序列改造方法及其在疫苗制备中的应用

Family Cites Families (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20050038609A1 (en) * 1992-03-25 2005-02-17 Benner Steven Albert Evolution-based functional genomics
WO1997033988A1 (en) * 1996-03-12 1997-09-18 Sloan-Kettering Institute For Cancer Research Double mutants of dihydrofolate reductase and methods of using same
US5783431A (en) * 1996-04-24 1998-07-21 Chromaxome Corporation Methods for generating and screening novel metabolic pathways
US6489141B1 (en) * 1997-07-09 2002-12-03 The University Of Queensland Nucleic acid sequence and methods for selectively expressing a protein in a target cell or tissue
AUPQ776100A0 (en) * 2000-05-26 2000-06-15 Australian National University, The Synthetic molecules and uses therefor
US7879540B1 (en) * 2000-08-24 2011-02-01 Promega Corporation Synthetic nucleic acid molecule compositions and methods of preparation
EP2305699B1 (de) * 2001-06-05 2014-08-13 CureVac GmbH Stabilisierte mRNA mit erhöhtem G/C-Gehalt und optimierter Codon Usage für die Impfung gegen Schlafkrankheit, Leishmaniose und Toxoplasmose
US20050069899A1 (en) * 2003-09-26 2005-03-31 Animal Technology Institute Taiwan Method of synthesizing a target polynucleotide efficiently expressed in a host-vector expression system
US20060177839A1 (en) * 2004-09-17 2006-08-10 Didier Mazel Method for modulating the evolution of a polypeptide encoded by a nucleic acid sequence

Also Published As

Publication number Publication date
DE602005017917D1 (de) 2010-01-07
EP1799823B1 (de) 2009-11-25
US20060177839A1 (en) 2006-08-10
EP1799823A2 (de) 2007-06-27
EP1799823B9 (de) 2010-09-15
US20080044825A1 (en) 2008-02-21
WO2006046132A2 (en) 2006-05-04
WO2006046132A3 (en) 2006-08-17

Similar Documents

Publication Publication Date Title
Schloter et al. Microbial indicators for soil quality
Jansson et al. The soil microbiome—from metagenomics to metaphenomics
Chen et al. Stochastic processes shape microeukaryotic community assembly in a subtropical river across wet and dry seasons
Niemiller et al. Recent divergence with gene flow in Tennessee cave salamanders (Plethodontidae: Gyrinophilus) inferred from gene genealogies
Linz et al. Freshwater carbon and nutrient cycles revealed through reconstructed population genomes
Schalamun et al. Harnessing the MinION: An example of how to establish long‐read sequencing in a laboratory using challenging plant tissue from Eucalyptus pauciflora
ES2595433T3 (es) Aumento de la confianza en las identificaciones de alelos con el recuento molecular
Vik et al. Putative archaeal viruses from the mesopelagic ocean
ATE438740T1 (de) Verfahren und nukleinsäuren zur analyse von mit der prognose von störungen der proliferation von prostatazellen assoziierter genexpression
WO2007019899A8 (en) Method for predicting the response to a treatment with a her dimerization inhibitor
Lankau et al. Ectomycorrhizal fungal richness declines towards the host species’ range edge
RU2006124515A (ru) СПОСОБ ПРОГНОЗИРОВАНИЯ ЭФФЕКТИВНОСТИ ИНТЕРФЕРИРУЮЩЕЙ РНК (PHKi)
Manganelli et al. Major role of microbes in carbon fluxes during austral winter in the Southern Drake Passage
CN103088120A (zh) 基于SLAFseq技术的大规模样品基因分型方法
Goh et al. Nipah shell disorder, modes of infection, and virulence
Carlini et al. Molecular genetic variation and population structure in morphologically differentiated cave and surface populations of the freshwater amphipod Gammarus minus
ATE449845T1 (de) Verfahren zur modulation der entwicklung eines durch eine nukleinsäuresequenz kodierten polypeptids
Zhang et al. GC bias lead to increased small amino acids and random coils of proteins in cold-water fishes
DE60324207D1 (de) Verfahren zur identifizierung und/oder validierung von rezeptortyrosinkinase-inhibitoren
US20120004115A1 (en) System and method for purification and use of inorganic pyrophosphatase from aquifex aeolicus
WO2003106674A3 (en) MEANS AND METHODS FOR REGULATING GENE EXPRESSION
Sun et al. Fighting climate change: soil bacteria communities and topography play a role in plant colonization of desert areas
ATE445009T1 (de) Signalpeptid zur herstellung eines polypeptids
ATE441728T1 (de) Mit cmv-resistenz assoziierter molekularer marker und verwendung davon
DE602004032114D1 (de) Rna-interferenz für das gen znfn3a1 als verfahren zur hemmung des wachstums von krebszellen

Legal Events

Date Code Title Description
RER Ceased as to paragraph 5 lit. 3 law introducing patent treaties