ATE435926T1 - Detektion von amplikon-kontamination während einer pcr die zwei verschiedene annealing- temperaturen aufweist - Google Patents
Detektion von amplikon-kontamination während einer pcr die zwei verschiedene annealing- temperaturen aufweistInfo
- Publication number
- ATE435926T1 ATE435926T1 AT05744996T AT05744996T ATE435926T1 AT E435926 T1 ATE435926 T1 AT E435926T1 AT 05744996 T AT05744996 T AT 05744996T AT 05744996 T AT05744996 T AT 05744996T AT E435926 T1 ATE435926 T1 AT E435926T1
- Authority
- AT
- Austria
- Prior art keywords
- reactions
- amplicone
- pcr
- detection
- annealing temperatures
- Prior art date
Links
- 238000011109 contamination Methods 0.000 title abstract 2
- 238000000137 annealing Methods 0.000 title 1
- 238000001514 detection method Methods 0.000 title 1
- 238000006243 chemical reaction Methods 0.000 abstract 6
- 230000003321 amplification Effects 0.000 abstract 2
- 238000003199 nucleic acid amplification method Methods 0.000 abstract 2
- 230000000295 complement effect Effects 0.000 abstract 1
- 238000000034 method Methods 0.000 abstract 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6844—Nucleic acid amplification reactions
- C12Q1/6848—Nucleic acid amplification reactions characterised by the means for preventing contamination or increasing the specificity or sensitivity of an amplification reaction
Landscapes
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Organic Chemistry (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Molecular Biology (AREA)
- General Engineering & Computer Science (AREA)
- Microbiology (AREA)
- Biotechnology (AREA)
- Biophysics (AREA)
- Analytical Chemistry (AREA)
- Physics & Mathematics (AREA)
- Biochemistry (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Immunology (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Control Of Temperature (AREA)
- Investigating Or Analyzing Materials By The Use Of Electric Means (AREA)
- Crystals, And After-Treatments Of Crystals (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| EP04090202A EP1598429A1 (de) | 2004-05-19 | 2004-05-19 | Detektion von Amplikon-Kontamination während einer PCR, die zwei verschiedene Annealing-Temperaturen aufweist |
| PCT/GB2005/001934 WO2005113803A1 (en) | 2004-05-19 | 2005-05-19 | Detection of amplicon contamination during pcr exhibiting two different annealing temperatures |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| ATE435926T1 true ATE435926T1 (de) | 2009-07-15 |
Family
ID=34928804
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| AT05744996T ATE435926T1 (de) | 2004-05-19 | 2005-05-19 | Detektion von amplikon-kontamination während einer pcr die zwei verschiedene annealing- temperaturen aufweist |
Country Status (6)
| Country | Link |
|---|---|
| US (1) | US7914986B2 (de) |
| EP (2) | EP1598429A1 (de) |
| JP (1) | JP2007537746A (de) |
| AT (1) | ATE435926T1 (de) |
| DE (1) | DE602005015315D1 (de) |
| WO (1) | WO2005113803A1 (de) |
Families Citing this family (47)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| PL2017356T3 (pl) * | 2006-06-06 | 2012-03-30 | Gen Probe Inc | Znakowane oligonukleotydy i ich zastosowanie w sposobach amplifikacji kwasu nukleinowego |
| US7833716B2 (en) | 2006-06-06 | 2010-11-16 | Gen-Probe Incorporated | Tagged oligonucleotides and their use in nucleic acid amplification methods |
| US8198027B2 (en) | 2006-12-21 | 2012-06-12 | Gen-Probe Incorporated | Methods and compositions for nucleic acid amplification |
| US8628927B2 (en) | 2008-11-07 | 2014-01-14 | Sequenta, Inc. | Monitoring health and disease status using clonotype profiles |
| US8748103B2 (en) | 2008-11-07 | 2014-06-10 | Sequenta, Inc. | Monitoring health and disease status using clonotype profiles |
| US8691510B2 (en) | 2008-11-07 | 2014-04-08 | Sequenta, Inc. | Sequence analysis of complex amplicons |
| EP2719774B8 (de) | 2008-11-07 | 2020-04-22 | Adaptive Biotechnologies Corporation | Verfahren zur Überwachung von Zuständen durch Sequenzanalyse |
| US9528160B2 (en) | 2008-11-07 | 2016-12-27 | Adaptive Biotechnolgies Corp. | Rare clonotypes and uses thereof |
| US9365901B2 (en) | 2008-11-07 | 2016-06-14 | Adaptive Biotechnologies Corp. | Monitoring immunoglobulin heavy chain evolution in B-cell acute lymphoblastic leukemia |
| US9506119B2 (en) | 2008-11-07 | 2016-11-29 | Adaptive Biotechnologies Corp. | Method of sequence determination using sequence tags |
| HUE029424T2 (en) | 2009-01-15 | 2017-02-28 | Adaptive Biotechnologies Corp | Adaptive immunity profiling and a method for producing monoclonal antibodies |
| RU2539032C2 (ru) | 2009-06-25 | 2015-01-10 | Фред Хатчинсон Кансэр Рисёч Сентер | Способ измерения искусственного иммунитета |
| WO2012032510A1 (en) | 2010-09-07 | 2012-03-15 | Yeda Research And Development Co. Ltd. | Primers for amplifying dna and methods of selecting same |
| ES2867924T3 (es) | 2011-12-09 | 2021-10-21 | Adaptive Biotechnologies Corp | Diagnóstico de tumores malignos linfoides y detección de enfermedad residual mínima |
| US9499865B2 (en) | 2011-12-13 | 2016-11-22 | Adaptive Biotechnologies Corp. | Detection and measurement of tissue-infiltrating lymphocytes |
| EP2823060B1 (de) | 2012-03-05 | 2018-02-14 | Adaptive Biotechnologies Corporation | Festlegung von gepaarten immunrezeptorketten aus frequenzabgestimmten untereinheiten |
| AU2013246050B2 (en) * | 2012-04-13 | 2017-03-16 | Adaptive Biotechnologies Corp. | Detection and quantitation of sample contamination in immune repertoire analysis |
| DK2831276T3 (da) | 2012-05-08 | 2016-08-01 | Adaptive Biotechnologies Corp | Sammensætninger og fremgangsmåde til at måle og kalibrere amplifikations-bias i multipleks-PCR-reaktioner |
| US20160002731A1 (en) | 2012-10-01 | 2016-01-07 | Adaptive Biotechnologies Corporation | Immunocompetence assessment by adaptive immune receptor diversity and clonality characterization |
| US9708657B2 (en) | 2013-07-01 | 2017-07-18 | Adaptive Biotechnologies Corp. | Method for generating clonotype profiles using sequence tags |
| WO2015029595A1 (ja) * | 2013-08-27 | 2015-03-05 | 株式会社日立ハイテクノロジーズ | 核酸分析装置およびその装置診断方法 |
| AU2015227054A1 (en) | 2014-03-05 | 2016-09-22 | Adaptive Biotechnologies Corporation | Methods using randomer-containing synthetic molecules |
| US10195610B2 (en) | 2014-03-10 | 2019-02-05 | Click Diagnostics, Inc. | Cartridge-based thermocycler |
| US10066265B2 (en) | 2014-04-01 | 2018-09-04 | Adaptive Biotechnologies Corp. | Determining antigen-specific t-cells |
| EP3132059B1 (de) | 2014-04-17 | 2020-01-08 | Adaptive Biotechnologies Corporation | Quantifizierung von adaptiven immunzellgenomen in einer komplexen mischung von zellen |
| CA2966201A1 (en) | 2014-10-29 | 2016-05-06 | Adaptive Biotechnologies Corp. | Highly-multiplexed simultaneous detection of nucleic acids encoding paired adaptive immune receptor heterodimers from many samples |
| JP6447810B2 (ja) * | 2014-10-31 | 2019-01-09 | セイコーエプソン株式会社 | 核酸検出方法 |
| US10246701B2 (en) | 2014-11-14 | 2019-04-02 | Adaptive Biotechnologies Corp. | Multiplexed digital quantitation of rearranged lymphoid receptors in a complex mixture |
| AU2015353581A1 (en) | 2014-11-25 | 2017-06-15 | Adaptive Biotechnologies Corporation | Characterization of adaptive immune response to vaccination or infection using immune repertoire sequencing |
| JP6778194B2 (ja) | 2014-12-31 | 2020-10-28 | ビスビュー メディカル，インコーポレイテッド | 分子診断試験のためのデバイス及び方法 |
| ES2858306T3 (es) | 2015-02-24 | 2021-09-30 | Adaptive Biotechnologies Corp | Método para determinar el estado de HLA mediante secuenciación del repertorio inmunitario |
| EP3277294B1 (de) | 2015-04-01 | 2024-05-15 | Adaptive Biotechnologies Corp. | Verfahren zur identifizierung von menschlichen kompatiblen t-zell-rezeptoren spezifisch für ein antigen-ziel |
| US10987674B2 (en) | 2016-04-22 | 2021-04-27 | Visby Medical, Inc. | Printed circuit board heater for an amplification module |
| WO2017197040A1 (en) | 2016-05-11 | 2017-11-16 | Click Diagnostics, Inc. | Devices and methods for nucleic acid extraction |
| CN110325652A (zh) | 2016-06-29 | 2019-10-11 | 易捷仪器诊断股份有限公司 | 使用流动池检测分子的装置和方法 |
| USD800331S1 (en) | 2016-06-29 | 2017-10-17 | Click Diagnostics, Inc. | Molecular diagnostic device |
| USD800913S1 (en) | 2016-06-30 | 2017-10-24 | Click Diagnostics, Inc. | Detection window for molecular diagnostic device |
| USD800914S1 (en) | 2016-06-30 | 2017-10-24 | Click Diagnostics, Inc. | Status indicator for molecular diagnostic device |
| US10428325B1 (en) | 2016-09-21 | 2019-10-01 | Adaptive Biotechnologies Corporation | Identification of antigen-specific B cell receptors |
| US11162130B2 (en) | 2017-11-09 | 2021-11-02 | Visby Medical, Inc. | Portable molecular diagnostic device and methods for the detection of target viruses |
| US11254980B1 (en) | 2017-11-29 | 2022-02-22 | Adaptive Biotechnologies Corporation | Methods of profiling targeted polynucleotides while mitigating sequencing depth requirements |
| KR20210081335A (ko) | 2018-09-03 | 2021-07-01 | 비스비 메디컬, 인코포레이티드 | 항생제 감수성 시험을 위한 장치 및 방법 |
| KR20220005009A (ko) | 2019-04-28 | 2022-01-12 | 비스비 메디컬, 인코포레이티드 | 디지털 검출 능력 및 무선 연결성을 갖는 분자 진단 디바이스 |
| US11352675B2 (en) | 2020-01-03 | 2022-06-07 | Visby Medical, Inc. | Devices and methods for antibiotic susceptability testing |
| MX2022012272A (es) * | 2020-03-31 | 2023-02-23 | Personal Genome Diagnostics Inc | Composiciones y metodos para determinar la calidad del acido nucleico. |
| USD1055307S1 (en) | 2021-08-13 | 2024-12-24 | Visby Medical, Inc. | Molecular diagnostic device |
| CN118256606A (zh) * | 2024-04-24 | 2024-06-28 | 青岛立见生物科技有限公司 | 一种核酸污染排查试剂 |
Family Cites Families (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| AU7653691A (en) * | 1990-04-05 | 1991-10-30 | United States of America, as represented by the Secretary, U.S. Department of Commerce, The | Modified rna template-specific polymerase chain reaction |
| CA2307177C (en) * | 1997-10-23 | 2004-06-29 | Exact Laboratories, Inc. | Methods for detecting contamination in molecular diagnostics using pcr |
| EP1203096B1 (de) * | 1999-07-28 | 2008-12-31 | Merck Serono Biodevelopment | Durchfluss-Mikroreaktor in dem lokale Temperaturzyklen auf eine fliessende Probe einwirken |
| CA2408574A1 (en) * | 2000-05-24 | 2001-11-29 | Micronics, Inc. | Microfluidic concentration gradient loop |
| GB2384308B (en) * | 2002-01-15 | 2003-12-24 | Matforsk Norwegian Food Res In | Methods of nucleic acid amplification |
-
2004
- 2004-05-19 EP EP04090202A patent/EP1598429A1/de not_active Withdrawn
-
2005
- 2005-05-19 JP JP2007517416A patent/JP2007537746A/ja active Pending
- 2005-05-19 WO PCT/GB2005/001934 patent/WO2005113803A1/en not_active Ceased
- 2005-05-19 US US11/596,703 patent/US7914986B2/en not_active Expired - Fee Related
- 2005-05-19 DE DE602005015315T patent/DE602005015315D1/de not_active Expired - Lifetime
- 2005-05-19 AT AT05744996T patent/ATE435926T1/de not_active IP Right Cessation
- 2005-05-19 EP EP05744996A patent/EP1749106B1/de not_active Expired - Lifetime
Also Published As
| Publication number | Publication date |
|---|---|
| WO2005113803A1 (en) | 2005-12-01 |
| DE602005015315D1 (de) | 2009-08-20 |
| US20090311672A1 (en) | 2009-12-17 |
| US7914986B2 (en) | 2011-03-29 |
| EP1749106A1 (de) | 2007-02-07 |
| EP1598429A1 (de) | 2005-11-23 |
| JP2007537746A (ja) | 2007-12-27 |
| EP1749106B1 (de) | 2009-07-08 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| ATE435926T1 (de) | Detektion von amplikon-kontamination während einer pcr die zwei verschiedene annealing- temperaturen aufweist | |
| DE60226500D1 (en) | Late-pcr | |
| WO2005089508A3 (en) | Dna sequence detection by limited primer extension | |
| WO2016154337A3 (en) | Method for identification and enumeration of nucleic acid sequences | |
| ATE418622T1 (de) | Polymorphismusnachweisverfahren | |
| NZ748011A (en) | Multiplex nucleic acid assay methods capable of detecting closely related alleles, and reagents therefor | |
| DE69308162D1 (de) | Methode zur amplification von ziel-gerichteten segmenten von nukleinsäure durch die ineinandergeschachtelte polymerase-kettenreaktion | |
| WO2004027082A3 (en) | Methods and compositions for detecting targets | |
| WO2010115044A3 (en) | Selective tagging of short nucleic acid fragments and selective protection of target sequences from degradation | |
| WO2001096612A3 (en) | Method of identifying and quantifying specific fungi and bacteria | |
| EP2551356A4 (de) | Verfahren zur erkennung von zielbasissequenzen mithilfe konkurrierender primer | |
| RU2018135256A (ru) | Композиции, способы и наборы для синтеза и обнаружения нуклеиновых кислот | |
| AU2009355009A8 (en) | THD primer target detection | |
| WO2014018958A3 (en) | Methods and devices for electromagnetic amplification of nucleic acids | |
| WO2008001376A3 (en) | Detection of analytes in a medium | |
| WO2019107893A3 (ko) | 표적핵산 증폭방법 및 표적핵산 증폭용 조성물 | |
| WO2004059289A3 (en) | Target-dependent transcription using deletion mutants of n4 rna polymerase | |
| EP2090653A4 (de) | Verfahren zum nachweis von apfelmosaikvirus, primersatz für den nachweis und kit für den nachweis | |
| Jung et al. | Isothermal target and signaling probe amplification method, based on a combination of an isothermal chain amplification technique and a fluorescence resonance energy transfer cycling probe technology | |
| ATE396282T1 (de) | Verfahren zum kontaminationsschutz von dna- amplifikationsreaktionen zur methylierungsanalyse durch modifizierte vorbehandlung der nukleinsäuren | |
| WO2003048308A3 (en) | Thermus brockianus nucleic acid polymerases | |
| Sasaki et al. | Rapid identification of Curcuma longa and C. aromatica by LAMP | |
| ATE403663T1 (de) | Nukleinsäureamplifikation mit duplexzwischenprodukten | |
| WO2015153571A3 (en) | Detection of dna methylation using combined nuclease ligation reactions | |
| EP1178119A3 (de) | Auf PCR beruhender Multiplextest zur Bestimmung des Haplotyps |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| RER | Ceased as to paragraph 5 lit. 3 law introducing patent treaties |